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CD124 Antibody (14-1249-82) in ICC/IF
Immunofluorescence analysis of Interleukin-4 receptor subunit alpha was performed using 70% confluent log phase PANC-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD124 Monoclonal Antibody (X2/45-12), eBioscience™ (Product # 14-1249-82) at 1:100 dilution in 0.1% BSA, incubated at 4 degrees celsius overnight, and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsor... View More
Product Details
14-1249-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
X2/45-12
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_467455
Product Specific Information
Description: The monoclonal antibody X2/45-12 reacts with human CD124, which is also known as the IL-4R alpha chain. CD124 can associate with the common gamma chain to form the type I IL-4R or with IL-13Ralpha1 to form the type II IL-4R. While both receptor types can bind IL-4, only the type II receptor can associate with IL-13. The type I IL-4 receptor is found on T, B, NK, mast cells, basophils, and macrophages. The type II IL-4 receptor is found on many non-hematopoietic cells and macrophages. CD124 contains an ITIM that associates with and activates STAT6. IL-4 is responsible for the generation of high-affinity IgE antibodies and driving the polarization of naive Th0 cells into Th2 cells. In mucosal barriers, the cytokine is involved in the recruitment of innate and adaptive immune effector cells and mucous overproduction. Finally, IL-4R also exists as a soluble form that serves primarily as a carrier protein for IL-4 that increases its half-life.
The X2/45 antibody has been reported to have antagonist activity.
Applications Reported: This X2/45-12 antibody has been reported for use in flow cytometric analysis, and western blotting. (Please use Functional Grade purified X2/45-12, Product # 16-1249-82, in functional assays).
Applications Tested: This X2/45-12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL4 Receptor alpha (IL4RA) is the alpha chain of the interleukin 4 receptor which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine and mucus production at sites of allergic inflammmation. The IL4 response is involved in promoting Th2 differentiation. The secreted extracellular domain of IL4R alpha, called sIL4R alpha, can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. It has no signaling abilities. Allelic variations in the IL4RA gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD124; il-4 receptor; IL-4 receptor subunit alpha; IL4R nirs variant 1; interleukin-4 receptor alpha chain; Interleukin-4 receptor subunit alpha
Gene Aliases: 582J2.1; CD124; IL-4RA; IL4R; IL4RA
UniProt ID: (Human) P24394
Entrez Gene ID: (Human) 3566
production of molecular mediator involved in inflammatory response
positive regulation of immunoglobulin production
immune response
signal transduction
immunoglobulin mediated immune response
ovulation
interleukin-4-mediated signaling pathway
regulation of cell proliferation
defense response to protozoan
positive regulation of macrophage activation
positive regulation of mast cell degranulation
response to estrogen
negative regulation of T-helper 1 cell differentiation
positive regulation of T-helper 2 cell differentiation
positive regulation of chemokine secretion
positive regulation of myoblast fusion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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