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Invitrogen
CD124 Monoclonal Antibody (X2/45-12), Functional Grade, eBioscience™
FIGURE: 1 / 2
CD124 Antibody (16-1249-82) in Flow
Surface staining of normal human peripheral blood cells with Anti-Human CD124 Purified followed by Anti-Mouse IgG Biotin and Streptavidin PE (Product # 12-4317-87). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
Product Details
16-1249-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
X2/45-12
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_469055
Product Specific Information
Description: The X2/45-12 monoclonal antibody reacts with the human IL-4 receptor alpha chain. IL-4 receptor alpha chain (CD124) is crucial for the binding and signal transduction of IL-4. Both IL-4 and IL-13 use the IL-4 receptor alpha chain as their receptor component and activate many common signal pathways.
Applications Reported: X2/45-12 has been reported for use in flow cytometric analysis. X2/45-12 has also been reported in neutralization of human IL-4 function.
Applications Tested: The X2/45-12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
IL4 Receptor alpha (IL4RA) is the alpha chain of the interleukin 4 receptor which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine and mucus production at sites of allergic inflammmation. The IL4 response is involved in promoting Th2 differentiation. The secreted extracellular domain of IL4R alpha, called sIL4R alpha, can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. It has no signaling abilities. Allelic variations in the IL4RA gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD124; il-4 receptor; IL-4 receptor subunit alpha; IL4R nirs variant 1; interleukin-4 receptor alpha chain; Interleukin-4 receptor subunit alpha
Gene Aliases: 582J2.1; CD124; IL-4RA; IL4R; IL4RA
UniProt ID: (Human) P24394
Entrez Gene ID: (Human) 3566
production of molecular mediator involved in inflammatory response
positive regulation of immunoglobulin production
immune response
signal transduction
immunoglobulin mediated immune response
ovulation
interleukin-4-mediated signaling pathway
regulation of cell proliferation
defense response to protozoan
positive regulation of macrophage activation
positive regulation of mast cell degranulation
response to estrogen
negative regulation of T-helper 1 cell differentiation
positive regulation of T-helper 2 cell differentiation
positive regulation of chemokine secretion
positive regulation of myoblast fusion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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