Invitrogen

IFN gamma Monoclonal Antibody (R4-6A2), eBioscience™

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IFN gamma Antibody (14-7312-85) in ELISA

JCI insight 2018 - Figure 1 Acox1 Lampe1 mutation promotes proinflammatory immune responsiveness. (A and B) Twelve-week-old, chow diet-fed, Acox1 Lampe1 mice and WT littermate controls were challenged i.p. with biotinylated capture Ab (20 mug/mouse) 3 hours prior to i.v. saline (NS) or LPS challenge (25 mug/mouse; ultrapure E. coli 0111:B4). Twenty-four hours later, serum was collected, and IL-6, TNF-alpha, IFN-gamma, and IL-... View More

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Product Details

14-7312-85

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 4 publications 4 publications

ELISA (ELISA)

View 19 publications 19 publications

Neutralization (Neu)

Assay-Dependent

View 7 publications 7 publications

Functional Assay (Functional)

Assay-Dependent

View 8 publications 8 publications

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

R4-6A2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468470

Product Specific Information

Description: The R4-6A2 antibody reacts with mouse interferon-gamma (IFN-gamma ), a homodimeric 15-20 kDa cytokine secreted by Th1 cells. The R4-6A2 antibody is a neutralizing antibody. IFN-gamma is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.

Applications Reported: The R4-6A2 antibody has been reported for use in cytokine neutralization, and immunoblotting (WB). The purified R4-6A2 antibody can be used for blocking of fluorochrome conjugated R4-6A2 in intracellular flow cytometry experiments to serve as a specificity control. It has also been reported to neutralize IFN gamma binding and biological activity. (Please use Functional Grade purified R4-6A2, Product # 16-7312, in functional assays).

Applications Tested: The Functional Grade purified R4-6A2 antibody has been tested for neutralization of mouse IFN gamma bioactivity.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo.

Molecular medicine reports

Huo J,Liu T,Li F,Song X,Hou X

Published figure using IFN gamma monoclonal antibody (Product # 14-7312-85) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Novel HLA-B7-restricted human metapneumovirus epitopes enhance viral clearance in mice and are recognized by human CD8⁺ T cells.

Scientific reports

Miranda-Katz M,Erickson JJ,Lan J,Ecker A,Zhang Y,Joyce S,Williams JV

Published figure using IFN gamma monoclonal antibody (Product # 14-7312-85) in ELISA

Thu Oct 21 00:00:00 EDT 2021

Qa-1-Restricted CD8⁺ T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection.

Cell reports

Anderson CK,Reilly EC,Lee AY,Brossay L

14-7312-85 was used in Flow Cytometry to study compensatory mechanisms in immune responses to viruses when classical T cell immunity has been evaded.

Tue Apr 09 00:00:00 EDT 2019

Peroxisomal β-oxidation regulates whole body metabolism, inflammatory vigor, and pathogenesis of nonalcoholic fatty liver disease.

JCI insight

Moreno-Fernandez ME,Giles DA,Stankiewicz TE,Sheridan R,Karns R,Cappelletti M,Lampe K,Mukherjee R,Sina C,Sallese A,Bridges JP,Hogan SP,Aronow BJ,Hoebe K,Divanovic S

Published figure using IFN gamma monoclonal antibody (Product # 14-7312-85) in ELISA

Thu Mar 22 00:00:00 EDT 2018

IL-6/STAT3 pathway induced deficiency of RFX1 contributes to Th17-dependent autoimmune diseases via epigenetic regulation.

Nature communications

Zhao M,Tan Y,Peng Q,Huang C,Guo Y,Liang G,Zhu B,Huang Y,Liu A,Wang Z,Li M,Gao X,Wu R,Wu H,Long H,Lu Q

14-7312 was used in Functional assays to show reduced expression of the transcription factor RFX1 in CD4+ T cells from patients with systemic lupus erythematosus which leads to IL-17A overexpression through increased histone H3 acetylation and decreased DNA methylation.

Thu Feb 08 00:00:00 EST 2018

Lamtor1 Is Critically Required for CD4⁺ T Cell Proliferation and Regulatory T Cell Suppressive Function.

Journal of immunology (Baltimore, Md. : 1950)

Hosokawa T,Kimura T,Nada S,Okuno T,Ito D,Kang S,Nojima S,Yamashita K,Nakatani T,Hayama Y,Kato Y,Kinehara Y,Nishide M,Mikami N,Koyama S,Takamatsu H,Okuzaki D,Ohkura N,Sakaguchi S,Okada M,Kumanogoh A

14-7312 was used in Functional assays to demonstrate that Lamtor1-deficient CD4(+) T cells exhibit marked reductions in proliferation, IL-2 production, Mechanistic target of rapamycin complex 1 activity, and expression of purine- and lipid-synthesis genes.

Fri Sep 15 00:00:00 EDT 2017

IFNGR1 signaling is associated with adverse pregnancy outcomes during infection with malaria parasites.

PloS one

Niikura M,Inoue SI,Mineo S,Asahi H,Kobayashi F

14-7312 was used in an ELISA assay to explore the role of IFN-γ receptor 1 signalling in placental pathology during severe malaria using luciferase-expressing rodent malaria parasites, P. berghei NK65.

Wed Nov 29 00:00:00 EST 2017

The DNA Structure-Specific Endonuclease MUS81 Mediates DNA Sensor STING-Dependent Host Rejection of Prostate Cancer Cells.

Immunity

Ho SS,Zhang WY,Tan NY,Khatoo M,Suter MA,Tripathi S,Cheung FS,Lim WK,Tan PH,Ngeow J,Gasser S

14-7312 was used in Blocking experiments to demonstrate that MUS81 alerts the immune system to the presence of transformed host cells.

Tue May 17 00:00:00 EDT 2016

Loss of the Protein Tyrosine Phosphatase PTPN22 Reduces Mannan-Induced Autoimmune Arthritis in SKG Mice.

Journal of immunology (Baltimore, Md. : 1950)

Sood S,Brownlie RJ,Garcia C,Cowan G,Salmond RJ,Sakaguchi S,Zamoyska R

14-7312 was used in Functional assays to show that small perturbations in TCR signal transduction pathways can have profound consequences on T cell differentiation and the development of autoimmune disease.

Fri Jul 15 00:00:00 EDT 2016

Identification of Candidate Tolerogenic CD8(+) T Cell Epitopes for Therapy of Type 1 Diabetes in the NOD Mouse Model.

Journal of diabetes research

Yu C,Burns JC,Robinson WH,Utz PJ,Ho PP,Steinman L,Frey AB

14-7312 was used in Flow cytometry/Cell sorting to investigate two predicted tolerogenic CD8(+) T cell epitopes from the NOD murine model that are pathogenic in type 1 diabetes.

Tue Dec 13 00:00:00 EST 2016

Different tissue distribution properties for glycosylation variants of fusion proteins containing the p40 subunit of murine interleukin-12.

Protein engineering, design & selection : PEDS

Bootz F,Venetz D,Ziffels B,Neri D

14-7312 was used in an ELISA assay to analyze biochemical and pharmaceutical properties of fusion proteins, consisting of the F8 antibody and the p40 subunit of interleukin-12.

Sat Oct 01 00:00:00 EDT 2016

Targeted Reconstitution of Cytokine Activity upon Antigen Binding using Split Cytokine Antibody Fusion Proteins.

The Journal of biological chemistry

Venetz D,Koovely D,Weder B,Neri D

14-7312 was used in an ELISA assay to demonstrate that interleukin-12 can be reconstituted by sequential reassembly of fusion proteins based on antibody fragments and interleukin-12 subunit mutants.

Fri Aug 26 00:00:00 EDT 2016

Targeting dendritic cells to accelerate T-cell activation overcomes a bottleneck in tuberculosis vaccine efficacy.

Nature communications

Griffiths KL,Ahmed M,Das S,Gopal R,Horne W,Connell TD,Moynihan KD,Kolls JK,Irvine DJ,Artyomov MN,Rangel-Moreno J,Khader SA

14-7312 was used in an ELISpot assay to demonstrate a novel method of pulmonary vaccine delivery using antigen-primed DCs for Tuberculosis infection.

Thu Dec 22 00:00:00 EST 2016

Helminth-induced arginase-1 exacerbates lung inflammation and disease severity in tuberculosis.

The Journal of clinical investigation

Monin L,Griffiths KL,Lam WY,Gopal R,Kang DD,Ahmed M,Rajamanickam A,Cruz-Lagunas A,Zúñiga J,Babu S,Kolls JK,Mitreva M,Rosa BA,Ramos-Payan R,Morrison TE,Murray PJ,Rangel-Moreno J,Pearce EJ,Khader SA

14-7312 was used in an ELISpot assay to indicate that helminth coinfection induces arginase-1-expressing type 2 granulomas, thereby increasing inflammation and TB disease severity.

Tue Dec 01 00:00:00 EST 2015

Caspase-1 independent viral clearance and adaptive immunity against mucosal respiratory syncytial virus infection.

Immune network

Shim YR,Lee HK

14-7312 was used in an ELISA assay to demonstrate that caspase-1 might not play a central role in the induction of Th1 and CTL immune responses to RSV.

Wed Apr 01 00:00:00 EDT 2015

Cigarette smoke exposure exacerbates lung inflammation and compromises immunity to bacterial infection.

Journal of immunology (Baltimore, Md. : 1950)

Lugade AA,Bogner PN,Thatcher TH,Sime PJ,Phipps RP,Thanavala Y

14-7312 was used in an ELISpot assay to demonstrate multiple mechanisms by which cigarette smoke exposure predisposes patients to infection and contributes to mortality.

Sun Jun 01 00:00:00 EDT 2014

Depletion of tumor-associated macrophages enhances the anti-tumor immunity induced by a Toll-like receptor agonist-conjugated peptide.

Human vaccines & immunotherapeutics

Shen KY,Song YC,Chen IH,Chong P,Liu SJ

14-7312 was used in an ELISpot assay to investigate whether di-palmitoylated peptides with TLR2 agonist activity are able to induce anti-tumour immunity, showing that depletion of tumour-associated macrophages enhances the anti-tumour immunity.

Fri Oct 23 00:00:00 EDT 2015

Identification of targets of CD8⁺ T cell responses to malaria liver stages by genome-wide epitope profiling.

PLoS pathogens

Hafalla JC,Bauza K,Friesen J,Gonzalez-Aseguinolaza G,Hill AV,Matuschewski K

14-7312 was used in Flow cytometry/Cell sorting to investigate antigenic epitopes in a murine malaria immunisation model.

Wed May 01 00:00:00 EDT 2013

Identification of targets of CD8⁺ T cell responses to malaria liver stages by genome-wide epitope profiling.

PLoS pathogens

Hafalla JC,Bauza K,Friesen J,Gonzalez-Aseguinolaza G,Hill AV,Matuschewski K

14-7312 was used in Flow cytometry/Cell sorting to investigate antigenic epitopes in a murine malaria immunisation model.

Wed May 01 00:00:00 EDT 2013

Induced IL-17-producing invariant NKT cells require activation in presence of TGF-β and IL-1β.

Journal of immunology (Baltimore, Md. : 1950)

Monteiro M,Almeida CF,Agua-Doce A,Graca L

14-7312 was used in Neutralization experiments to demonstrate that IL-17 production by iNKT cells can be imprinted in natural iNKT17 cells or peripherally induced.

Tue Jan 15 00:00:00 EST 2013

Th17 cells induce Th1-polarizing monocyte-derived dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Davidson MG,Alonso MN,Yuan R,Axtell RC,Kenkel JA,Suhoski MM,González JC,Steinman L,Engleman EG

14-7312 was used in Functional assays to investigate how murine Th cells influence the phenotype and function of monocytes.

Thu Aug 01 00:00:00 EDT 2013

Development of severe pathology in immunized pregnant mice challenged with lethal malaria parasites.

Infection and immunity

Mineo S,Niikura M,Inoue S,Kuroda M,Kobayashi F

14-7312 was used in an ELISA assay to investigate the pathogenesis of maternal malaria.

Tue Oct 01 00:00:00 EDT 2013

Autophagy protects against active tuberculosis by suppressing bacterial burden and inflammation.

Proceedings of the National Academy of Sciences of the United States of America

Castillo EF,Dekonenko A,Arko-Mensah J,Mandell MA,Dupont N,Jiang S,Delgado-Vargas M,Timmins GS,Bhattacharya D,Yang H,Hutt J,Lyons CR,Dobos KM,Deretic V

14-7312 was used in an ELISA assay to study the role of autophagy during immune responses to tuberculosis.

Tue Nov 13 00:00:00 EST 2012

A20 (Tnfaip3) deficiency in myeloid cells protects against influenza A virus infection.

PLoS pathogens

Maelfait J,Roose K,Bogaert P,Sze M,Saelens X,Pasparakis M,Carpentier I,van Loo G,Beyaert R

14-7312 was used in an ELISA assay to examine the role of the ubiquitin-editing protein A20 in the innate immune responses to IAV and other viruses.

Mon Jul 09 00:00:00 EDT 2012

Osteoclast activated FoxP3+ CD8+ T-cells suppress bone resorption in vitro.

PloS one

Buchwald ZS,Kiesel JR,DiPaolo R,Pagadala MS,Aurora R

14-7312 was used in Flow cytometry/Cell sorting to demonstrate the effects of activated CD8 T cell-produced cytokines on osteoclast activity.

Thu Nov 08 00:00:00 EST 2012

Microbiota-induced tertiary lymphoid tissues aggravate inflammatory disease in the absence of RORgamma t and LTi cells.

The Journal of experimental medicine

Lochner M,Ohnmacht C,Presley L,Bruhns P,Si-Tahar M,Sawa S,Eberl G

14-7312 was used in Neutralization experiments to investigate potential for intestinal immunodeficiency compensation by increased lymphoid tissue genesis.

Mon Jan 17 00:00:00 EST 2011

Programmed death (PD)-1-deficient mice are extremely sensitive to murine hepatitis virus strain-3 (MHV-3) infection.

PLoS pathogens

Chen Y,Wu S,Guo G,Fei L,Guo S,Yang C,Fu X,Wu Y

14-7312 was used in Neutralization experiments to investigate the functional mechanisms of PD-1 as related to fulminant viral hepatitis pathogenesis.

Fri Jul 01 00:00:00 EDT 2011

TSLP enhances the function of helper type 2 cells.

European journal of immunology

Kitajima M,Lee HC,Nakayama T,Ziegler SF

14-7312 was used in Functional assays to investigate the direct effect of TSLP on effector T cells.

Fri Jul 01 00:00:00 EDT 2011

Absence of β2 integrins impairs regulatory T cells and exacerbates CD4+ T cell-dependent autoimmune carditis.

Journal of immunology (Baltimore, Md. : 1950)

Haasken S,Auger JL,Binstadt BA

14-7312 was used in Immunohistochemistry to investigate the contribution of CD4(+) T cells and β(2) integrins in the pathogenesis of autoimmune arthritis and endocarditis.

Thu Sep 01 00:00:00 EDT 2011

Pyridone 6, a pan-JAK inhibitor, ameliorates allergic skin inflammation of NC/Nga mice via suppression of Th2 and enhancement of Th17.

Journal of immunology (Baltimore, Md. : 1950)

Nakagawa R,Yoshida H,Asakawa M,Tamiya T,Inoue N,Morita R,Inoue H,Nakao A,Yoshimura A

14-7312 was used in Neutralization experiments to suggest that the JAK inhibitor P6 is therapeutic for atopic dermatitis by modulating the balance of Th2 and Th17.

Tue Nov 01 00:00:00 EDT 2011

Interleukin-17 contributes to generation of Th1 immunity and neutrophil recruitment during Chlamydia muridarum genital tract infection but is not required for macrophage influx or normal resolution of infection.

Infection and immunity

Scurlock AM,Frazer LC,Andrews CW,O'Connell CM,Foote IP,Bailey SL,Chandra-Kuntal K,Kolls JK,Darville T

14-7312 was used in an ELISpot assay to investigate the role of IL-17 during C. muridarum genital tract infection.

Tue Mar 01 00:00:00 EST 2011

In vivo requirement for Atg5 in antigen presentation by dendritic cells.

Immunity

Lee HK,Mattei LM,Steinberg BE,Alberts P,Lee YH,Chervonsky A,Mizushima N,Grinstein S,Iwasaki A

14-7312 was used in an ELISpot assay to investigate how dendritic cells utilise autophagic machinery to optimally present extracellular microbial antigens for MHC II molecules.

Fri Feb 26 00:00:00 EST 2010

Immune responses to coiled coil supramolecular biomaterials.

Biomaterials

Rudra JS,Tripathi PK,Hildeman DA,Jung JP,Collier JH

14-7312 was used in an ELISA assay to investigate murine immune responses to a set of self-oligomerization assembled biomaterials.

Mon Nov 01 00:00:00 EDT 2010

CD30 ligand/CD30 plays a critical role in Th17 differentiation in mice.

Journal of immunology (Baltimore, Md. : 1950)

Sun X,Yamada H,Shibata K,Muta H,Tani K,Podack ER,Yoshikai Y

14-7312 was used in Flow cytometry/Cell sorting to elucidate a role for CD30 in T cell differentiation.

Sun Aug 15 00:00:00 EDT 2010

CD4+ T cells elicit host immune responses to MHC class II-negative ovarian cancer through CCL5 secretion and CD40-mediated licensing of dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Nesbeth YC,Martinez DG,Toraya S,Scarlett UK,Cubillos-Ruiz JR,Rutkowski MR,Conejo-Garcia JR

14-7312 was used in an ELISpot assay to investigate how to incorporate tumor-reactive CD4(+) T cells in adoptive cell transfer immunotherapies against ovarian cancer.

Sat May 15 00:00:00 EDT 2010

A candidate H1N1 pandemic influenza vaccine elicits protective immunity in mice.

PloS one

Steitz J,Barlow PG,Hossain J,Kim E,Okada K,Kenniston T,Rea S,Donis RO,Gambotto A

14-7312 was used in an ELISpot assay to show the potential of an adenoviral-based influenza vaccine, benefiting from the efficacy of a single dose immunization, in response to the H1N1 pandemic.

Wed May 05 00:00:00 EDT 2010

Mucosal immunotherapy for protection from pneumonic infection with Francisella tularensis.

Vaccine

Troyer RM,Propst KL,Fairman J,Bosio CM,Dow SW

14-7312 was used in Neutralization experiments to conclude that activation of local pulmonary innate immune responses is capable of eliciting significant protection from inhalational exposure to a virulent bacterial pathogen.

Thu Jul 16 00:00:00 EDT 2009

Protection from pneumonic infection with burkholderia species by inhalational immunotherapy.

Infection and immunity

Goodyear A,Kellihan L,Bielefeldt-Ohmann H,Troyer R,Propst K,Dow S

14-7312 was used in an ELISA assay to study how the burkholderia species protects from pneumonic infection by inhalational immunotherapy.

Wed Apr 01 00:00:00 EDT 2009

A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1.

Cell research

Sheng H,Wang Y,Jin Y,Zhang Q,Zhang Y,Wang L,Shen B,Yin S,Liu W,Cui L,Li N

14-7312 was used in Flow cytometry/Cell sorting to investigate the role that IFNγ plays in mesenchymal stem cell mediated suppression of T cell proliferation.

Fri Aug 01 00:00:00 EDT 2008

Efficient presentation of myelin oligodendrocyte glycoprotein peptides but not protein by astrocytes from HLA-DR2 and HLA-DR4 transgenic mice.

Journal of neuroimmunology

Kort JJ,Kawamura K,Fugger L,Weissert R,Forsthuber TG

Sat Apr 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

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Gene Aliases: Ifg; IFN-g; Ifng

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UniProt ID: (Mouse) P01580

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Entrez Gene ID: (Mouse) 15978

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Function(s)

cytokine activity interferon-gamma receptor binding protein binding cytokine interferon superfamily intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IFN gamma Monoclonal Antibody (R4-6A2), eBioscience™

IFN gamma Antibody (14-7312-85) in ELISA

Product Details

14-7312-85

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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