Invitrogen

IFN gamma Monoclonal Antibody (R4-6A2), Functional Grade, eBioscience™

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IFN gamma Antibody (16-7312-81) in ELISA

Oncoimmunology 2021 - Published figure using IFN gamma monoclonal antibody (Product # 16-7312-81) in ELISA

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Product Details

16-7312-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

View 2 publications 2 publications

ELISA (ELISA)

View 6 publications 6 publications

Neutralization (Neu)

Assay-Dependent

View 2 publications 2 publications

Functional Assay (Functional)

Assay-Dependent

View 2 publications 2 publications

Inhibition Assays (IA)

View 1 publication 1 publication

T-Cell Activation (TCA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

R4-6A2

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469244

Product Specific Information

Description: The R4-6A2 antibody reacts with mouse interferon-gamma (IFN-gamma ), a homodimeric 15-20 kDa cytokine secreted by Th1 cells. The R4-6A2 antibody is a neutralizing antibody. IFN-gamma is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.

Applications Reported: The R4-6A2 antibody has been reported for use in cytokine neutralization.

Applications Tested: The Functional Grade Purified R4-6A2 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested in bioassay for neutralization of IFN gamma bioactivity.

The R4-6A2 antibody at 15 ng/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse IFN gamma (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation.

Cellular & molecular immunology

Liu Y,Bockermann R,Hadi M,Safari I,Carrion B,Kveiborg M,Issazadeh-Navikas S

16-7312-81 was used in Functional assay to study the role of ADAM12 in T-cell responses, fate determination on activation, and its functions in T cells to mediate tissue inflammation.

Sun Aug 01 00:00:00 EDT 2021

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo.

Molecular medicine reports

Huo J,Liu T,Li F,Song X,Hou X

Published figure using IFN gamma monoclonal antibody (Product # 16-7312-81) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Novel HLA-B7-restricted human metapneumovirus epitopes enhance viral clearance in mice and are recognized by human CD8⁺ T cells.

Scientific reports

Miranda-Katz M,Erickson JJ,Lan J,Ecker A,Zhang Y,Joyce S,Williams JV

Published figure using IFN gamma monoclonal antibody (Product # 16-7312-81) in ELISA

Thu Oct 21 00:00:00 EDT 2021

Chromatin accessibility governs the differential response of cancer and T cells to arginine starvation.

Cell reports

Crump NT,Hadjinicolaou AV,Xia M,Walsby-Tickle J,Gileadi U,Chen JL,Setshedi M,Olsen LR,Lau IJ,Godfrey L,Quek L,Yu Z,Ballabio E,Barnkob MB,Napolitani G,Salio M,Koohy H,Kessler BM,Taylor S,Vyas P,McCullagh JSO,Milne TA,Cerundolo V

16-7312-81 was used in ELISA to show that tumor cells synthesize arginine from citrulline by upregulating argininosuccinate synthetase 1 (ASS1).

Tue May 11 00:00:00 EDT 2021

Gasdermin D in peripheral myeloid cells drives neuroinflammation in experimental autoimmune encephalomyelitis.

The Journal of experimental medicine

Li S,Wu Y,Yang D,Wu C,Ma C,Liu X,Moynagh PN,Wang B,Hu G,Yang S

16-7312-81 was used in Flow Cytometry to further define the role of gasdermin D in experimental autoimmune encephalomyelitis.

Mon Nov 04 00:00:00 EST 2019

The RNA-binding protein HuR contributes to neuroinflammation by promoting C-C chemokine receptor 6 (CCR6) expression on Th17 cells.

The Journal of biological chemistry

Chen J,Martindale JL,Cramer C,Gorospe M,Atasoy U,Drew PD,Yu S

16-7312 was used inT-Cell Activation experiments to study how HuR contributes to T helper 17 cell-mediated autoimmune neuroinflammation, and its potential therapeutic target for managing autoimmune conditions of the central nervous system.

Fri Sep 01 00:00:00 EDT 2017

CD1b-autoreactive T cells recognize phospholipid antigens and contribute to antitumor immunity against a CD1b⁺ T cell lymphoma.

Oncoimmunology

Bagchi S,Li S,Wang CR

Published figure using IFN gamma monoclonal antibody (Product # 16-7312-81) in ELISA

Tue Jan 12 00:00:00 EST 2021

Intestinal intraepithelial lymphocyte activation promotes innate antiviral resistance.

Nature communications

Swamy M,Abeler-Dörner L,Chettle J,Mahlakõiv T,Goubau D,Chakravarty P,Ramsay G,Reis e Sousa C,Staeheli P,Blacklaws BA,Heeney JL,Hayday AC

16-7312 was used in Blocking experiments to demonstrate that activated intestinal intraepithelial lymphocytes cause the functional upregulation of antiviral interferon-responsive genes.

Tue May 19 00:00:00 EDT 2015

Severe influenza A(H1N1)pdm09 infection induces thymic atrophy through activating innate CD8(+)CD44(hi) T cells by upregulating IFN-γ.

Cell death & disease

Liu B,Zhang X,Deng W,Liu J,Li H,Wen M,Bao L,Qu J,Liu Y,Li F,An Y,Qin C,Cao B,Wang C

16-7312 was used in Neutralization experiments to demonstrate that thymic innate CD8(+)CD44(hi) single positive T-cells have critical roles in influenza A(H1N1)pdm09 infection-induced thymic atrophy through secreting IFN-γ.

Thu Oct 02 00:00:00 EDT 2014

The kinetics of myelin antigen uptake by myeloid cells in the central nervous system during experimental autoimmune encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Sosa RA,Murphey C,Ji N,Cardona AE,Forsthuber TG

16-7312 was used in an ELISpot assay to investigate the kinetics of myelin Ag uptake by CNS APCs during experimental autoimmune encephalomyelitis and in the naive CNS.

Sun Dec 15 00:00:00 EST 2013

Immunotherapy markedly increases the effectiveness of antimicrobial therapy for treatment of Burkholderia pseudomallei infection.

Antimicrobial agents and chemotherapy

Propst KL,Troyer RM,Kellihan LM,Schweizer HP,Dow SW

16-7312 was used in Functional assays to investigate whether active immunotherapy with IFN-gamma could enhance therapy for B. pseudomallei infection.

Sat May 01 00:00:00 EDT 2010

Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization.

Journal of immunology (Baltimore, Md. : 1950)

Weber KS,Hildner K,Murphy KM,Allen PM

16-7312 was used in an ELISpot assay to study the regulation of unique Th cell subset calcium signals and their correlation to biological functions, showing that Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization.

Wed Sep 01 00:00:00 EDT 2010

Role of the T cell in the genesis of angiotensin II induced hypertension and vascular dysfunction.

The Journal of experimental medicine

Guzik TJ,Hoch NE,Brown KA,McCann LA,Rahman A,Dikalov S,Goronzy J,Weyand C,Harrison DG

16-7312 was used in Neutralization experiments to identify a role for T cells in hypertension and support a role of inflammation in the basis of hypertension.

Mon Oct 01 00:00:00 EDT 2007

Efficient presentation of myelin oligodendrocyte glycoprotein peptides but not protein by astrocytes from HLA-DR2 and HLA-DR4 transgenic mice.

Journal of neuroimmunology

Kort JJ,Kawamura K,Fugger L,Weissert R,Forsthuber TG

Sat Apr 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

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Gene Aliases: Ifg; IFN-g; Ifng

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UniProt ID: (Mouse) P01580

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Entrez Gene ID: (Mouse) 15978

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Function(s)

cytokine activity interferon-gamma receptor binding protein binding cytokine interferon superfamily intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IFN gamma Monoclonal Antibody (R4-6A2), Functional Grade, eBioscience™

IFN gamma Antibody (16-7312-81) in ELISA

Product Details

16-7312-81

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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