Invitrogen

SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), eBioscience™

28 Published Figures

27 References

View all (76) SSEA1 (CD15) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (28)

FIGURE: 1 / 29

SSEA1 Antibody (14-8813-82) in Flow

Staining of the F9 cell line with 0.25 µg of Mouse IgM Isotype Control Purified (Product # 14-4752-82) (open histogram) or 0.25 µg Anti-Human/Mouse SSEA-1 Purified (filled histogram) followed by Anti-Mouse IgM PE (Product # 12-5790-82).Total viable cells were used for analysis.

SSEA1 Antibody in Immunocytochemistry (ICC/IF)

SSEA1 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

SSEA1 Antibody in Immunocytochemistry, Immunohistochemistry, Flow Cytometry (ICC/IF, IHC, Flow)

SSEA1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

SSEA1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

14-8813-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 7 publications 7 publications

Flow Cytometry (Flow)

0.5 µg/test

View 15 publications 15 publications

Immunoprecipitation (IP)

Assay-Dependent

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

eBioMC-480 (MC-480)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657617

Product Specific Information

Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation.

Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.

Applications Tested: This eBioMC-480 (MC-480) antibody has been tested by flow cytometric analysis of the F9 cell line. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

High-throughput screening in postimplantation haploid epiblast stem cells reveals Hs3st3b1 as a modulator for reprogramming.

Stem cells translational medicine

Gao Q,Zhang W,Zhao Y,Tian Y,Wang Y,Zhang J,Geng M,Xu M,Yao C,Wang H,Li L,Liu Y,Shuai L

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

ECE2 regulates neurogenesis and neuronal migration during human cortical development.

EMBO reports

Buchsbaum IY,Kielkowski P,Giorgio G,O'Neill AC,Di Giaimo R,Kyrousi C,Khattak S,Sieber SA,Robertson SP,Cappello S

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Flow Cytometry

Wed May 06 00:00:00 EDT 2020

Comparative glycoproteomics of stem cells identifies new players in ricin toxicity.

Nature

Stadlmann J,Taubenschmid J,Wenzel D,Gattinger A,Dürnberger G,Dusberger F,Elling U,Mach L,Mechtler K,Penninger JM

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Immunocytochemistry

Thu Sep 28 00:00:00 EDT 2017

A vital sugar code for ricin toxicity.

Cell research

Taubenschmid J,Stadlmann J,Jost M,Klokk TI,Rillahan CD,Leibbrandt A,Mechtler K,Paulson JC,Jude J,Zuber J,Sandvig K,Elling U,Marquardt T,Thiel C,Koerner C,Penninger JM

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

A vital sugar code for ricin toxicity.

Cell research

Taubenschmid J,Stadlmann J,Jost M,Klokk TI,Rillahan CD,Leibbrandt A,Mechtler K,Paulson JC,Jude J,Zuber J,Sandvig K,Elling U,Marquardt T,Thiel C,Koerner C,Penninger JM

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Noncanonical function of DGCR8 controls mESC exit from pluripotency.

The Journal of cell biology

Cirera-Salinas D,Yu J,Bodak M,Ngondo RP,Herbert KM,Ciaudo C

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Foxd3 Promotes Exit from Naive Pluripotency through Enhancer Decommissioning and Inhibits Germline Specification.

Cell stem cell

Respuela P,Nikolić M,Tan M,Frommolt P,Zhao Y,Wysocka J,Rada-Iglesias A

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2016

The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features.

Scientific reports

Debowski K,Drummer C,Lentes J,Cors M,Dressel R,Lingner T,Salinas-Riester G,Fuchs S,Sasaki E,Behr R

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Immunohistochemistry

Thu Jul 07 00:00:00 EDT 2016

Alternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage.

Stem cell reports

Jackson SA,Olufs ZP,Tran KA,Zaidan NZ,Sridharan R

14-8813 was used in Immunofluorescence to investigate alternative routes of cellular reprogramming.

Tue Mar 08 00:00:00 EST 2016

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Immunocytochemistry

Thu Jul 30 00:00:00 EDT 2015

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Immunocytochemistry

Thu Jul 30 00:00:00 EDT 2015

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Immunocytochemistry

Thu Jul 30 00:00:00 EDT 2015

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Immunocytochemistry

Thu Jul 30 00:00:00 EDT 2015

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Immunocytochemistry

Thu Jul 30 00:00:00 EDT 2015

Injury induces direct lineage segregation of functionally distinct airway basal stem/progenitor cell subpopulations.

Cell stem cell

Pardo-Saganta A,Law BM,Tata PR,Villoria J,Saez B,Mou H,Zhao R,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Immunofluorescence

Thu Feb 05 00:00:00 EST 2015

Coexpression analysis of CD133 and CD44 identifies proneural and mesenchymal subtypes of glioblastoma multiforme.

Oncotarget

Brown DV,Daniel PM,D'Abaco GM,Gogos A,Ng W,Morokoff AP,Mantamadiotis T

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Flow Cytometry

Fri Mar 20 00:00:00 EDT 2015

Distinct and combinatorial functions of Jmjd2b/Kdm4b and Jmjd2c/Kdm4c in mouse embryonic stem cell identity.

Molecular cell

Das PP,Shao Z,Beyaz S,Apostolou E,Pinello L,De Los Angeles A,O'Brien K,Atsma JM,Fujiwara Y,Nguyen M,Ljuboja D,Guo G,Woo A,Yuan GC,Onder T,Daley G,Hochedlinger K,Kim J,Orkin SH

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Flow Cytometry

Thu Jan 09 00:00:00 EST 2014

Dax1 and Nanog act in parallel to stabilize mouse embryonic stem cells and induced pluripotency.

Nature communications

Zhang J,Liu G,Ruan Y,Wang J,Zhao K,Wan Y,Liu B,Zheng H,Peng T,Wu W,He P,Hu FQ,Jian R

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Flow Cytometry

Mon Oct 06 00:00:00 EDT 2014

Dedifferentiation of committed epithelial cells into stem cells in vivo.

Nature

Tata PR,Mou H,Pardo-Saganta A,Zhao R,Prabhu M,Law BM,Vinarsky V,Cho JL,Breton S,Sahay A,Medoff BD,Rajagopal J

14-8813 was used in Immunofluorescence to show that airway epithelial cells can dedifferentiate into stable and functional stem cells, suggesting that they could be used for tissue regeneration.

Thu Nov 14 00:00:00 EST 2013

Dedifferentiation of committed epithelial cells into stem cells in vivo.

Nature

Tata PR,Mou H,Pardo-Saganta A,Zhao R,Prabhu M,Law BM,Vinarsky V,Cho JL,Breton S,Sahay A,Medoff BD,Rajagopal J

14-8813 was used in Immunofluorescence to show that airway epithelial cells can dedifferentiate into stable and functional stem cells, suggesting that they could be used for tissue regeneration.

Thu Nov 14 00:00:00 EST 2013

Cell reprogramming requires silencing of a core subset of polycomb targets.

PLoS genetics

Fragola G,Germain PL,Laise P,Cuomo A,Blasimme A,Gross F,Signaroldi E,Bucci G,Sommer C,Pruneri G,Mazzarol G,Bonaldi T,Mostoslavsky G,Casola S,Testa G

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Cell reprogramming requires silencing of a core subset of polycomb targets.

PLoS genetics

Fragola G,Germain PL,Laise P,Cuomo A,Blasimme A,Gross F,Signaroldi E,Bucci G,Sommer C,Pruneri G,Mazzarol G,Bonaldi T,Mostoslavsky G,Casola S,Testa G

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Cell reprogramming requires silencing of a core subset of polycomb targets.

PLoS genetics

Fragola G,Germain PL,Laise P,Cuomo A,Blasimme A,Gross F,Signaroldi E,Bucci G,Sommer C,Pruneri G,Mazzarol G,Bonaldi T,Mostoslavsky G,Casola S,Testa G

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

An ex vivo gene therapy approach to treat muscular dystrophy using inducible pluripotent stem cells.

Nature communications

Filareto A,Parker S,Darabi R,Borges L,Iacovino M,Schaaf T,Mayerhofer T,Chamberlain JS,Ervasti JM,McIvor RS,Kyba M,Perlingeiro RC

14-8813 was used in Flow cytometry/Cell sorting to show the regenerative potential of myogenic progenitors derived from corrected dystrophic induced pluripotent stem cells of dystrophin and utrophin deficient mice.

Thu Jun 20 00:00:00 EDT 2013

FGF2 mediates mouse spermatogonial stem cell self-renewal via upregulation of Etv5 and Bcl6b through MAP2K1 activation.

Development (Cambridge, England)

Ishii K,Kanatsu-Shinohara M,Toyokuni S,Shinohara T

14-8813 was used in Flow cytometry/Cell sorting to assess the role of the FGF2 signalling pathway for SSC self-renewal.

Tue May 01 00:00:00 EDT 2012

Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells.

Nature cell biology

ten Berge D,Kurek D,Blauwkamp T,Koole W,Maas A,Eroglu E,Siu RK,Nusse R

Published figure using SSEA1 monoclonal antibody (Product # 14-8813-82) in Flow Cytometry

Sun Aug 14 00:00:00 EDT 2011

Dynamic changes in EPCAM expression during spermatogonial stem cell differentiation in the mouse testis.

PloS one

Kanatsu-Shinohara M,Takashima S,Ishii K,Shinohara T

14-8813 was used in Flow cytometry/Cell sorting to show that EPCAM plays an important role in progenitor cell amplification in the mouse spermatogenic system.

Wed Feb 15 00:00:00 EST 2012

Type 3 inositol 1,4,5-trisphosphate receptor negatively regulates apoptosis during mouse embryonic stem cell differentiation.

Cell death and differentiation

Liang J,Wang YJ,Tang Y,Cao N,Wang J,Yang HT

14-8813 was used in Immunocytochemistry to show the role of IP(3)R3 in the regulation of apoptosis in early differentiating embryonic stem cells and subsequent lineage commitments.

Thu Jul 01 00:00:00 EDT 2010

Three-dimensional cartography of hematopoietic clusters in the vasculature of whole mouse embryos.

Development (Cambridge, England)

Yokomizo T,Dzierzak E

14-8813 was used in Immunohistochemistry to devise a method to make transparent whole mouse embryos for the observation of hematopoietic clusters.

Mon Nov 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: 3-fucosyl-N-acetyl-lactosamine epitope; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid specific; alpha (1,3) fucosyltransferase, myeloid-specific; alpha 1,3-fucosyltransferase; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1

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Gene Aliases: AI451562; CD15; ELFT; FAL; FCT3A; FUC-TIV; FucT-IV; FUT4; FUTIV; LeX; SSEA-1; Ssea1

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UniProt ID: (Human) P22083, (Mouse) Q11127

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Entrez Gene ID: (Human) 2526, (Mouse) 14345

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Function(s)

Process(es)

carbohydrate metabolic process protein glycosylation protein N-linked glycosylation fucosylation L-fucose catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), eBioscience™

SSEA1 Antibody (14-8813-82) in Flow

Product Details

14-8813-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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