Invitrogen

SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), Alexa Fluor™ 488, eBioscience™

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Antibody Testing Data (1)
Published Figures (25)

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SSEA1 Antibody (53-8813-42) in Flow

Staining of F9 cells with Mouse IgM Isotype Control FITC (Product # 11-4752-80) (blue histogram) or 0.125 µg of Anti-Human/Mouse SSEA-1 Alexa Fluor® 488 (purple histogram). Total viable cells were used for analysis.

SSEA1 Antibody in Immunocytochemistry (ICC/IF)

SSEA1 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

SSEA1 Antibody in Immunocytochemistry, Immunohistochemistry, Flow Cytometry (ICC/IF, IHC, Flow)

SSEA1 Antibody in Immunohistochemistry (IHC)

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Product Details

53-8813-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

eBioMC-480 (MC-480)

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10596519

Product Specific Information

Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation.

Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by flow cytometric analysis of F9 cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

High-throughput screening in postimplantation haploid epiblast stem cells reveals Hs3st3b1 as a modulator for reprogramming.

Stem cells translational medicine

Gao Q,Zhang W,Zhao Y,Tian Y,Wang Y,Zhang J,Geng M,Xu M,Yao C,Wang H,Li L,Liu Y,Shuai L

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

ECE2 regulates neurogenesis and neuronal migration during human cortical development.

EMBO reports

Buchsbaum IY,Kielkowski P,Giorgio G,O'Neill AC,Di Giaimo R,Kyrousi C,Khattak S,Sieber SA,Robertson SP,Cappello S

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Wed May 06 00:00:00 EDT 2020

ECE2 regulates neurogenesis and neuronal migration during human cortical development.

EMBO reports

Buchsbaum IY,Kielkowski P,Giorgio G,O'Neill AC,Di Giaimo R,Kyrousi C,Khattak S,Sieber SA,Robertson SP,Cappello S

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Wed May 06 00:00:00 EDT 2020

A transcriptome-wide antitermination mechanism sustaining identity of embryonic stem cells.

Nature communications

Kainov YA,Makeyev EV

53-8813-42 was used in Flow cytometry/Cell sorting to show that the RNA-associated protein Srrt/Ars2 sustains embryonic stem cell identity by preventing premature termination of numerous transcripts at cryptic cleavage/polyadenylation sites in first introns.

Fri Jan 17 00:00:00 EST 2020

Comparative glycoproteomics of stem cells identifies new players in ricin toxicity.

Nature

Stadlmann J,Taubenschmid J,Wenzel D,Gattinger A,Dürnberger G,Dusberger F,Elling U,Mach L,Mechtler K,Penninger JM

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Immunocytochemistry

Thu Sep 28 00:00:00 EDT 2017

A vital sugar code for ricin toxicity.

Cell research

Taubenschmid J,Stadlmann J,Jost M,Klokk TI,Rillahan CD,Leibbrandt A,Mechtler K,Paulson JC,Jude J,Zuber J,Sandvig K,Elling U,Marquardt T,Thiel C,Koerner C,Penninger JM

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

A vital sugar code for ricin toxicity.

Cell research

Taubenschmid J,Stadlmann J,Jost M,Klokk TI,Rillahan CD,Leibbrandt A,Mechtler K,Paulson JC,Jude J,Zuber J,Sandvig K,Elling U,Marquardt T,Thiel C,Koerner C,Penninger JM

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Noncanonical function of DGCR8 controls mESC exit from pluripotency.

The Journal of cell biology

Cirera-Salinas D,Yu J,Bodak M,Ngondo RP,Herbert KM,Ciaudo C

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.

Nature

Murakami K,Günesdogan U,Zylicz JJ,Tang WWC,Sengupta R,Kobayashi T,Kim S,Butler R,Dietmann S,Surani MA

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Thu Jan 21 00:00:00 EST 2016

The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features.

Scientific reports

Debowski K,Drummer C,Lentes J,Cors M,Dressel R,Lingner T,Salinas-Riester G,Fuchs S,Sasaki E,Behr R

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Immunohistochemistry

Thu Jul 07 00:00:00 EDT 2016

Alternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage.

Stem cell reports

Jackson SA,Olufs ZP,Tran KA,Zaidan NZ,Sridharan R

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Immunofluorescence

Tue Mar 08 00:00:00 EST 2016

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2015

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2015

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2015

Injury induces direct lineage segregation of functionally distinct airway basal stem/progenitor cell subpopulations.

Cell stem cell

Pardo-Saganta A,Law BM,Tata PR,Villoria J,Saez B,Mou H,Zhao R,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Immunofluorescence

Thu Feb 05 00:00:00 EST 2015

Coexpression analysis of CD133 and CD44 identifies proneural and mesenchymal subtypes of glioblastoma multiforme.

Oncotarget

Brown DV,Daniel PM,D'Abaco GM,Gogos A,Ng W,Morokoff AP,Mantamadiotis T

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Fri Mar 20 00:00:00 EDT 2015

Distinct and combinatorial functions of Jmjd2b/Kdm4b and Jmjd2c/Kdm4c in mouse embryonic stem cell identity.

Molecular cell

Das PP,Shao Z,Beyaz S,Apostolou E,Pinello L,De Los Angeles A,O'Brien K,Atsma JM,Fujiwara Y,Nguyen M,Ljuboja D,Guo G,Woo A,Yuan GC,Onder T,Daley G,Hochedlinger K,Kim J,Orkin SH

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Thu Jan 09 00:00:00 EST 2014

Dax1 and Nanog act in parallel to stabilize mouse embryonic stem cells and induced pluripotency.

Nature communications

Zhang J,Liu G,Ruan Y,Wang J,Zhao K,Wan Y,Liu B,Zheng H,Peng T,Wu W,He P,Hu FQ,Jian R

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Mon Oct 06 00:00:00 EDT 2014

Cell reprogramming requires silencing of a core subset of polycomb targets.

PLoS genetics

Fragola G,Germain PL,Laise P,Cuomo A,Blasimme A,Gross F,Signaroldi E,Bucci G,Sommer C,Pruneri G,Mazzarol G,Bonaldi T,Mostoslavsky G,Casola S,Testa G

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Cell reprogramming requires silencing of a core subset of polycomb targets.

PLoS genetics

Fragola G,Germain PL,Laise P,Cuomo A,Blasimme A,Gross F,Signaroldi E,Bucci G,Sommer C,Pruneri G,Mazzarol G,Bonaldi T,Mostoslavsky G,Casola S,Testa G

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Cell reprogramming requires silencing of a core subset of polycomb targets.

PLoS genetics

Fragola G,Germain PL,Laise P,Cuomo A,Blasimme A,Gross F,Signaroldi E,Bucci G,Sommer C,Pruneri G,Mazzarol G,Bonaldi T,Mostoslavsky G,Casola S,Testa G

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells.

Nature cell biology

ten Berge D,Kurek D,Blauwkamp T,Koole W,Maas A,Eroglu E,Siu RK,Nusse R

Published figure using SSEA1 monoclonal antibody (Product # 53-8813-42) in Flow Cytometry

Sun Aug 14 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 3-fucosyl-N-acetyl-lactosamine epitope; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid specific; alpha (1,3) fucosyltransferase, myeloid-specific; alpha 1,3-fucosyltransferase; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1

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Gene Aliases: AI451562; CD15; ELFT; FAL; FCT3A; FUC-TIV; FucT-IV; FUT4; FUTIV; LeX; SSEA-1; Ssea1

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UniProt ID: (Human) P22083, (Mouse) Q11127

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Entrez Gene ID: (Human) 2526, (Mouse) 14345

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Function(s)

Process(es)

carbohydrate metabolic process protein glycosylation protein N-linked glycosylation fucosylation L-fucose catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), Alexa Fluor™ 488, eBioscience™

SSEA1 Antibody (53-8813-42) in Flow

Product Details

53-8813-42

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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