Invitrogen

CD40 Monoclonal Antibody (1C10), PE-Cyanine5, eBioscience™

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CD40 Antibody (15-0401-82) in Flow

Staining of C57Bl/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 µg Anti-Mouse CD40 PE-Cyanine5 (filled histogram).Total cells were used for analysis.

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Product Details

15-0401-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 36 publications 36 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1C10

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468747

Product Specific Information

Description: The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.

The monoclonal antibody 1C10 is reported to have agonistic activity in vitro and in vivo.

Applications Reported: The 1C10 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 1C10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitis.

Neural regeneration research

Liu C,Guo S,Liu R,Guo M,Wang Q,Chai Z,Xiao B,Ma C

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2024

Prostaglandin E2 serves a dual role in regulating the migration of dendritic cells.

International journal of molecular medicine

Diao G,Huang J,Zheng X,Sun X,Tian M,Han J,Guo J

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Myeloid and plasmacytoid dendritic cell combined vaccines loaded with heat-treated tumor cell lysates enhance antitumor activity in murine lung cancer.

Oncology letters

Chen H,Tan J,Li X,Li H,Wu W,Wu Y,Zhang J,Gu L

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.

Frontiers in immunology

Agalave NM,Mody PH,Szabo-Pardi TA,Jeong HS,Burton MD

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Mon Sep 20 00:00:00 EDT 2021

Modulation of lactate-lysosome axis in dendritic cells by clotrimazole potentiates antitumor immunity.

Journal for immunotherapy of cancer

Wang Z,Xu F,Hu J,Zhang H,Cui L,Lu W,He W,Wang X,Li M,Zhang H,Xiong W,Xie C,Liu Y,Zhou P,Liu J,Huang P,Qin XF,Xia X

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Induction of autophagy through CLEC4E in combination with TLR4: an innovative strategy to restrict the survival of Mycobacterium tuberculosis.

Autophagy

Pahari S,Negi S,Aqdas M,Arnett E,Schlesinger LS,Agrewala JN

15-0401-82 was used in Flow Cytometry to invesigate if a unique immunotherapeutic approach involving CLEC4E in conjunction with TLR4 to restrict the survival of mycobacterium tuberculosis through autophagy exists.

Mon Jun 01 00:00:00 EDT 2020

Silencing IDO2 in dendritic cells: A novel strategy to strengthen cancer immunotherapy in a murine lung cancer model.

International journal of oncology

Liu Y,Xu P,Liu H,Fang C,Guo H,Chen X,Tan M,Zhang Y,Min W

15-0401-82 was used in Flow Cytometry to provide evidence that the silencing of the tryptophan rate‚limiting metabolic enzyme, IDO2, has the potential to enhance the efficacy of DC‚based cancer immunotherapy.

Sat Aug 01 00:00:00 EDT 2020

E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions.

International journal of oncology

Huang J,Diao G,Zhang Q,Chen Y,Han J,Guo J

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2020

Polyanhydride Nanoparticles Induce Low Inflammatory Dendritic Cell Activation Resulting in CD8⁺ T Cell Memory and Delayed Tumor Progression.

International journal of nanomedicine

Darling R,Senapati S,Christiansen J,Liu L,Ramer-Tait AE,Narasimhan B,Wannemuehler M

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

cGAS/STING axis mediates a topoisomerase II inhibitor-induced tumor immunogenicity.

The Journal of clinical investigation

Wang Z,Chen J,Hu J,Zhang H,Xu F,He W,Wang X,Li M,Lu W,Zeng G,Zhou P,Huang P,Chen S,Li W,Xia LP,Xia X

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Tue Aug 13 00:00:00 EDT 2019

Efficient oral vaccination by bioengineering virus-like particles with protozoan surface proteins.

Nature communications

Serradell MC,Rupil LL,Martino RA,Prucca CG,Carranza PG,Saura A,Fernández EA,Gargantini PR,Tenaglia AH,Petiti JP,Tonelli RR,Reinoso-Vizcaino N,Echenique J,Berod L,Piaggio E,Bellier B,Sparwasser T,Klatzmann D,Luján HD

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Mon Jan 21 00:00:00 EST 2019

Bryostatin-1 alleviates experimental multiple sclerosis.

Proceedings of the National Academy of Sciences of the United States of America

Kornberg MD,Smith MD,Shirazi HA,Calabresi PA,Snyder SH,Kim PM

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Tue Feb 27 00:00:00 EST 2018

FcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells.

Frontiers in immunology

Pan YG,Yu YL,Lin CC,Lanier LL,Chu CL

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Engaging the CD40-CD40L pathway augments T-helper cell responses and improves control of Mycobacterium tuberculosis infection.

PLoS pathogens

Sia JK,Bizzell E,Madan-Lala R,Rengarajan J

15-0401 was used in Flow cytometry/Cell sorting to determine the mechanism by which dendritic cells generate antigen specific T helper cells in response to Mycobacterium tuberculosis infection.

Tue Aug 01 00:00:00 EDT 2017

CD40-mediated signalling influences trafficking, T-cell receptor expression, and T-cell pathogenesis, in the NOD model of type 1 diabetes.

Immunology

Vaitaitis GM,Waid DM,Yussman MG,Wagner DH

15-0401-82 was used in Flow Cytometry to determine the mechanism by which CD40 results in the pathogenesis of type 1 diabetes.

Sun Oct 01 00:00:00 EDT 2017

Discrimination of the heterogeneity of bone marrow‑derived dendritic cells.

Molecular medicine reports

Wang J,Dai X,Hsu C,Ming C,He Y,Zhang J,Wei L,Zhou P,Wang CY,Yang J,Gong N

Wed Nov 01 00:00:00 EDT 2017

Batf3-dependent CD8α⁺ Dendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.

EBioMedicine

Li Y,Liu X,Duan W,Tian H,Zhu G,He H,Yao S,Yi S,Song W,Tang H

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Paeoniflorin Ameliorates Experimental Autoimmune Encephalomyelitis via Inhibition of Dendritic Cell Function and Th17 Cell Differentiation.

Scientific reports

Zhang H,Qi Y,Yuan Y,Cai L,Xu H,Zhang L,Su B,Nie H

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Mon Feb 06 00:00:00 EST 2017

Tumor-derived exosomes educate dendritic cells to promote tumor metastasis via HSP72/HSP105-TLR2/TLR4 pathway.

Oncoimmunology

Shen Y,Guo D,Weng L,Wang S,Ma Z,Yang Y,Wang P,Wang J,Cai Z

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Combination immunotherapy with TLR agonists and checkpoint inhibitors suppresses head and neck cancer.

JCI insight

Sato-Kaneko F,Yao S,Ahmadi A,Zhang SS,Hosoya T,Kaneda MM,Varner JA,Pu M,Messer KS,Guiducci C,Coffman RL,Kitaura K,Matsutani T,Suzuki R,Carson DA,Hayashi T,Cohen EE

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Thu Sep 21 00:00:00 EDT 2017

Nuclear receptor expression atlas in BMDCs: Nr4a2 restricts immunogenicity of BMDCs and impedes EAE.

European journal of immunology

Saini A,Mahajan S,Gupta P

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Repulsive guidance molecule a blockade exerts the immunoregulatory function in DCs stimulated with ABP and LPS.

Human vaccines & immunotherapeutics

Xu X,Gao Y,Zhai Z,Zhang S,Shan F,Feng J

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Tue Aug 02 00:00:00 EDT 2016

MyD88/CD40 Genetic Adjuvant Function in Cutaneous Atypical Antigen-Presenting Cells Contributes to DNA Vaccine Immunogenicity.

PloS one

Collinson-Pautz MR,Slawin KM,Levitt JM,Spencer DM

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Wed May 31 00:00:00 EDT 2017

D-Alanylation of Teichoic Acids and Loss of Poly-N-Acetyl Glucosamine in Staphylococcus aureus during Exponential Growth Phase Enhance IL-12 Production in Murine Dendritic Cells.

PloS one

Lund LD,Ingmer H,Frøkiær H

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Thu Jul 14 00:00:00 EDT 2016

Adjuvants may reduce in vivo transfection levels for DNA vaccination in mice leading to reduced antigen-specific CD8+ T cell responses.

Human vaccines & immunotherapeutics

Rose AH,Hoffmann FW,Hara JH,Urschitz J,Moisyadi S,Hoffmann PR,Bertino P

15-0401 was used in Flow cytometry/Cell sorting to evaluate the adjuvant properties of cationic liposomes, Adjuplex and in vivo-JetPEI compared to injection of DNA without adjuvant.

Mon May 16 00:00:00 EDT 2016

CD40 ligand preferentially modulates immune response and enhances protection against influenza virus.

Journal of immunology (Baltimore, Md. : 1950)

Hashem AM,Gravel C,Chen Z,Yi Y,Tocchi M,Jaentschke B,Fan X,Li C,Rosu-Myles M,Pereboev A,He R,Wang J,Li X

15-0401 was used in Flow cytometry/Cell sorting to characterise an increased in vivo load of CD40-targeted Ag upon the secretion of the NP-CD40L fusion protein from adenovirus-infected cells.

Tue Jul 15 00:00:00 EDT 2014

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Mon Jan 05 00:00:00 EST 2015

Deficiency of autophagy in dendritic cells protects against experimental autoimmune encephalomyelitis.

The Journal of biological chemistry

Bhattacharya A,Parillon X,Zeng S,Han S,Eissa NT

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Fri Sep 19 00:00:00 EDT 2014

Resistance to malaria by enhanced phagocytosis of erythrocytes in LMP7-deficient mice.

PloS one

Duan X,Imai T,Chou B,Tu L,Himeno K,Suzue K,Hirai M,Taniguchi T,Okada H,Shimokawa C,Hisaeda H

15-0401 was used in Flow cytometry/Cell sorting to study how RBCs of LMP7-deficient mice were more likely to deform in response to infection with malaria parasites.

Tue Sep 17 00:00:00 EDT 2013

Nonviral delivery of small interfering RNA into pancreas-associated immune cells prevents autoimmune diabetes.

Molecular therapy : the journal of the American Society of Gene Therapy

Leconet W,Petit P,Peraldi-Roux S,Bresson D

15-0401 was used in Flow cytometry/Cell sorting to provide new insights into the development of siRNA-based therapeutics for the treatment of T1D.

Sat Dec 01 00:00:00 EST 2012

Impaired innate immunity in mice deficient in interleukin-1 receptor-associated kinase 4 leads to defective type 1 T cell responses, B cell expansion, and enhanced susceptibility to infection with Toxoplasma gondii.

Infection and immunity

Béla SR,Dutra MS,Mui E,Montpetit A,Oliveira FS,Oliveira SC,Arantes RM,Antonelli LR,McLeod R,Gazzinelli RT

15-0401 was used in Flow cytometry/Cell sorting to investigate the role of IRAK4 in Toxoplasma gondii infection, showing that deficiency leads to impaired innate immunity.

Sat Dec 01 00:00:00 EST 2012

Diabetic mice display a delayed adaptive immune response to Mycobacterium tuberculosis.

Journal of immunology (Baltimore, Md. : 1950)

Vallerskog T,Martens GW,Kornfeld H

15-0401 was used in Flow cytometry/Cell sorting to conclude that TB increased susceptibility in DM results from a delayed innate immune response to the presence of M. tuberculosis-infected alveolar macrophages.

Tue Jun 01 00:00:00 EDT 2010

Identification of regulatory functions for 4-1BB and 4-1BBL in myelopoiesis and the development of dendritic cells.

Nature immunology

Lee SW,Park Y,So T,Kwon BS,Cheroutre H,Mittler RS,Croft M

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2008

Dynamics of lung macrophage activation in response to helminth infection.

Journal of leukocyte biology

Siracusa MC,Reece JJ,Urban JF,Scott AL

Published figure using CD40 monoclonal antibody (Product # 15-0401-82) in Flow Cytometry

Mon Dec 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40L receptor; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; T-cell differentiation antigen; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

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Gene Aliases: AI326936; Bp50; Cd40; GP39; HIGM1; IGM; IMD3; p50; T-BAM; Tnfrsf5; TRAP

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UniProt ID: (Mouse) Q99NE2

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Entrez Gene ID: (Mouse) 21939

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Function(s)

antigen binding protein binding enzyme binding ubiquitin protein ligase binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD40 Monoclonal Antibody (1C10), PE-Cyanine5, eBioscience™

CD40 Antibody (15-0401-82) in Flow

Product Details

15-0401-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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