Invitrogen

CD11c Monoclonal Antibody (3.9), APC, eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (13)

FIGURE: 1 / 14

CD11c Antibody (17-0116-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD11c APC (purple histogram). Cells in the monocyte gate were used for analysis.

Product Image Gallery

Product Details

17-0116-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3.9

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1659668

Product Specific Information

Description: The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alphaX chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions.

Applications Reported: This 3.9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD11, along with CD18, form a heterodimer adhesion molecule. In particular, CD11 is composed of CD11a, CD11b and CD11c. CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes. CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Alveolar macrophages from EVALI patients and e-cigarette users: a story of shifting phenotype.

Respiratory research

Warren KJ,Beck EM,Callahan SJ,Helms MN,Middleton E,Maddock S,Carr JR,Harris D,Blagev DP,Lanspa MJ,Brown SM,Paine R

Published figure using CD11c monoclonal antibody (Product # 17-0116-42) in Flow Cytometry

Sat Jun 17 00:00:00 EDT 2023

Aluminum hydroxide adjuvant diverts the uptake and trafficking of genetically detoxified pertussis toxin to lysosomes in macrophages.

Molecular microbiology

Jaldin-Fincati J,Moussaoui S,Gimenez MC,Ho CY,Lancaster CE,Botelho R,Ausar F,Brookes R,Terebiznik M

Published figure using CD11c monoclonal antibody (Product # 17-0116-42) in Flow Cytometry

Sun May 01 00:00:00 EDT 2022

Upregulation of CD3ζ and L-selectin in antigen-specific cytotoxic T lymphocytes by eliminating myeloid-derived suppressor cells with doxorubicin to improve killing efficacy of neuroblastoma cells in vitro.

Journal of clinical laboratory analysis

Xu W,Li S,Li M,Zhou H,Yang X

Published figure using CD11c monoclonal antibody (Product # 17-0116-42) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2022

Isolevuglandins disrupt PU.1-mediated C1q expression and promote autoimmunity and hypertension in systemic lupus erythematosus.

JCI insight

Patrick DM,de la Visitación N,Krishnan J,Chen W,Ormseth MJ,Stein CM,Davies SS,Amarnath V,Crofford LJ,Williams JM,Zhao S,Smart CD,Dikalov S,Dikalova A,Xiao L,Van Beusecum JP,Ao M,Fogo AB,Kirabo A,Harrison DG

Published figure using CD11c monoclonal antibody (Product # 17-0116-42) in Flow Cytometry

Fri Jul 08 00:00:00 EDT 2022

Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus.

Journal of immunology research

Satria RD,Huang TW,Jhan MK,Shen TJ,Tseng PC,Wang YT,Yang ZY,Hsing CH,Lin CF

Published figure using CD11c monoclonal antibody (Product # 17-0116-42) in Flow Cytometry

Mon Dec 06 00:00:00 EST 2021

SUMOylation disassembles the tetrameric pyruvate kinase M2 to block myeloid differentiation of leukemia cells.

Cell death & disease

Xia L,Jiang Y,Zhang XH,Wang XR,Wei R,Qin K,Lu Y

Published figure using CD11c monoclonal antibody (Product # 17-0116-42) in Flow Cytometry

Wed Jan 20 00:00:00 EST 2021

The long non-coding RNA Cancer Susceptibility 15 (CASC15) is induced by isocitrate dehydrogenase (IDH) mutations and maintains an immature phenotype in adult acute myeloid leukemia.

Haematologica

Grasedieck S,Ruess C,Krowiorz K,Lux S,Pochert N,Schwarzer A,Klusmann JH,Jongen-Lavrencic M,Herold T,Bullinger L,Pollack JR,Rouhi A,Kuchenbauer F

17-0116 was used in Flow cytometry/Cell sorting to sequence stranded, non-poly-A-enriched cDNA libraries that were prepared from healthy human donor bone marrow.

Tue Sep 01 00:00:00 EDT 2020

The long non-coding RNA Cancer Susceptibility 15 (CASC15) is induced by isocitrate dehydrogenase (IDH) mutations and maintains an immature phenotype in adult acute myeloid leukemia.

Haematologica

Grasedieck S,Ruess C,Krowiorz K,Lux S,Pochert N,Schwarzer A,Klusmann JH,Jongen-Lavrencic M,Herold T,Bullinger L,Pollack JR,Rouhi A,Kuchenbauer F

17-0116 was used in Flow cytometry/Cell sorting to sequence stranded, non-poly-A-enriched cDNA libraries that were prepared from healthy human donor bone marrow.

Tue Sep 01 00:00:00 EDT 2020

Human and Mouse Transcriptome Profiling Identifies Cross-Species Homology in Pulmonary and Lymph Node Mononuclear Phagocytes.

Cell reports

Leach SM,Gibbings SL,Tewari AD,Atif SM,Vestal B,Danhorn T,Janssen WJ,Wager TD,Jakubzick CV

Published figure using CD11c monoclonal antibody (Product # 17-0116-42) in Flow Cytometry

Tue Nov 03 00:00:00 EST 2020

AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.

Oncology letters

Xiu W,Ma J,Lei T,Zhang M

Published figure using CD11c monoclonal antibody (Product # 17-0116-42) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Dengue immune sera enhance Zika virus infection in human peripheral blood monocytes through Fc gamma receptors.

PloS one

Li M,Zhao L,Zhang C,Wang X,Hong W,Sun J,Liu R,Yu L,Wang J,Zhang F,Jin X

17-0116 was used in Flow cytometry/Cell sorting to examine the mechanisms of antibody dependent enhancement of Zika virus infection in primary human cells.

Fri Jan 11 00:00:00 EST 2019

Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis.

Frontiers in immunology

Lopes DM,Oliveira SC,Page B,Carvalho LP,Carvalho EM,Cardoso LS

17-0116 was used in Flow cytometry/Cell sorting to evaluate the potential of the S.mansoni Sm29 antigen to change aspects of profiles of monocyte derived dendritic cells and lymphocytes from subjects with cutaneous leishmaniasis.

Tue Oct 15 00:00:00 EDT 2019

Challenging PD-L1 expressing cytotoxic T cells as a predictor for response to immunotherapy in melanoma.

Nature communications

Brochez L,Meireson A,Chevolet I,Sundahl N,Ost P,Kruse V

17-0116 was used in Flow cytometry/Cell sorting to study the association of PD-L1-expressing cytotoxic T cells with an altered immune climate and a negative impact on disease outcome in melanoma patients independent of treatment.

Thu Jul 26 00:00:00 EDT 2018

Global survey of the immunomodulatory potential of common drugs.

Nature chemical biology

Vladimer GI,Snijder B,Krall N,Bigenzahn JW,Huber KVM,Lardeau CH,Sanjiv K,Ringler A,Berglund UW,Sabler M,de la Fuente OL,Knöbl P,Kubicek S,Helleday T,Jäger U,Superti-Furga G

17-0116 was used in Flow cytometry/Cell sorting to offer an attractive platform for the personalized identification and characterization of immunomodulatory therapeutics.

Thu Jun 01 00:00:00 EDT 2017

Pharmacological Inhibition of the Histone Lysine Demethylase KDM1A Suppresses the Growth of Multiple Acute Myeloid Leukemia Subtypes.

Cancer research

McGrath JP,Williamson KE,Balasubramanian S,Odate S,Arora S,Hatton C,Edwards TM,O'Brien T,Magnuson S,Stokoe D,Daniels DL,Bryant BM,Trojer P

17-0116 was used in Flow cytometry/Cell sorting to investigate the consequences of inhibiting lysine-specific demethylase 1 in a panel of cell lines representing all acute myelogenous leukaemia subtypes.

Fri Apr 01 00:00:00 EDT 2016

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using CD11c monoclonal antibody (Product # 17-0116-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Pharmacological targeting of miR-155 via the NEDD8-activating enzyme inhibitor MLN4924 (Pevonedistat) in FLT3-ITD acute myeloid leukemia.

Leukemia

Khalife J,Radomska HS,Santhanam R,Huang X,Neviani P,Saultz J,Wang H,Wu YZ,Alachkar H,Anghelina M,Dorrance A,Curfman J,Bloomfield CD,Medeiros BC,Perrotti D,Lee LJ,Lee RJ,Caligiuri MA,Pichiorri F,Croce CM,Garzon R,Guzman ML,Mendler JH,Marcucci G

Published figure using CD11c monoclonal antibody (Product # 17-0116-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

17-0116 was used in Flow cytometry/Cell sorting to demonstrate amelioration of experimental autoimmune encephalomyelitis by a blood brain barrier permeable peptide to deliver ctCTLA-4 protein.

Tue Sep 15 00:00:00 EDT 2015

Clinical significance of plasmacytoid dendritic cells and myeloid-derived suppressor cells in melanoma.

Journal of translational medicine

Chevolet I,Speeckaert R,Schreuer M,Neyns B,Krysko O,Bachert C,Van Gele M,van Geel N,Brochez L

17-0116 was used in Flow cytometry/Cell sorting to investigate multiple immune cell types in a series of blood samples from melanoma patients with different stages of the disease.

Fri Jan 16 00:00:00 EST 2015

Expansion of CMV-mediated NKG2C+ NK cells associates with the development of specific de novo malignancies in liver-transplanted patients.

Journal of immunology (Baltimore, Md. : 1950)

Achour A,Baychelier F,Besson C,Arnoux A,Marty M,Hannoun L,Samuel D,Debré P,Vieillard V

17-0116 was used in Flow cytometry/Cell sorting to study the relationship between the modulation of natural killer cells and cytomegalovirus status after liver transplant.

Wed Jan 01 00:00:00 EST 2014

Dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients.

BioMed research international

Lopes DM,Fernandes JS,Cardoso TM,Bafica AM,Oliveira SC,Carvalho EM,Araujo MI,Cardoso LS

17-0116 was used in Flow cytometry/Cell sorting to investigate the dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients.

Thu Jul 02 00:00:00 EDT 2015

Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

The Journal of clinical investigation

Radomska HS,Alberich-Jordà M,Will B,Gonzalez D,Delwel R,Tenen DG

Published figure using CD11c monoclonal antibody (Product # 17-0116-42) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD11 antigen-like family member C; CD11c; complement component 3 receptor 4 subunit; integrin alpha X; Integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); Leu M5; leu M5, alpha subunit; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain

View more View less

Gene Aliases: CD11C; ITGAX; SLEB6

View more View less

UniProt ID: (Human) P20702

View more View less

Entrez Gene ID: (Human) 3687

View more View less

Function(s)

receptor activity protein binding metal ion binding integrin

Process(es)

cell adhesion integrin-mediated signaling pathway organ morphogenesis extracellular matrix organization heterotypic cell-cell adhesion leukocyte migration positive regulation of protein targeting to mitochondrion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD11c Monoclonal Antibody (3.9), APC, eBioscience™

CD11c Antibody (17-0116-42) in Flow

Product Details

17-0116-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!