Invitrogen

CD11c Monoclonal Antibody (3.9), PerCP-eFluor™ 710, eBioscience™

13 Published Figures

18 References

View all (154) CD11c antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (13)

FIGURE: 1 / 14

CD11c Antibody (46-0116-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or Anti-Human CD11c PerCP-eFluor® 710 (purple histogram). Cells in the monocyte gate were used for analysis.

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Product Details

46-0116-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3.9

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10596368

Product Specific Information

Description: The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alphaX chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions.

Applications Reported: This 3.9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD11, along with CD18, form a heterodimer adhesion molecule. In particular, CD11 is composed of CD11a, CD11b and CD11c. CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes. CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Alveolar macrophages from EVALI patients and e-cigarette users: a story of shifting phenotype.

Respiratory research

Warren KJ,Beck EM,Callahan SJ,Helms MN,Middleton E,Maddock S,Carr JR,Harris D,Blagev DP,Lanspa MJ,Brown SM,Paine R

Published figure using CD11c monoclonal antibody (Product # 46-0116-42) in Flow Cytometry

Sat Jun 17 00:00:00 EDT 2023

Aluminum hydroxide adjuvant diverts the uptake and trafficking of genetically detoxified pertussis toxin to lysosomes in macrophages.

Molecular microbiology

Jaldin-Fincati J,Moussaoui S,Gimenez MC,Ho CY,Lancaster CE,Botelho R,Ausar F,Brookes R,Terebiznik M

Published figure using CD11c monoclonal antibody (Product # 46-0116-42) in Flow Cytometry

Sun May 01 00:00:00 EDT 2022

Upregulation of CD3ζ and L-selectin in antigen-specific cytotoxic T lymphocytes by eliminating myeloid-derived suppressor cells with doxorubicin to improve killing efficacy of neuroblastoma cells in vitro.

Journal of clinical laboratory analysis

Xu W,Li S,Li M,Zhou H,Yang X

Published figure using CD11c monoclonal antibody (Product # 46-0116-42) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2022

Isolevuglandins disrupt PU.1-mediated C1q expression and promote autoimmunity and hypertension in systemic lupus erythematosus.

JCI insight

Patrick DM,de la Visitación N,Krishnan J,Chen W,Ormseth MJ,Stein CM,Davies SS,Amarnath V,Crofford LJ,Williams JM,Zhao S,Smart CD,Dikalov S,Dikalova A,Xiao L,Van Beusecum JP,Ao M,Fogo AB,Kirabo A,Harrison DG

Published figure using CD11c monoclonal antibody (Product # 46-0116-42) in Flow Cytometry

Fri Jul 08 00:00:00 EDT 2022

Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus.

Journal of immunology research

Satria RD,Huang TW,Jhan MK,Shen TJ,Tseng PC,Wang YT,Yang ZY,Hsing CH,Lin CF

Published figure using CD11c monoclonal antibody (Product # 46-0116-42) in Flow Cytometry

Mon Dec 06 00:00:00 EST 2021

SUMOylation disassembles the tetrameric pyruvate kinase M2 to block myeloid differentiation of leukemia cells.

Cell death & disease

Xia L,Jiang Y,Zhang XH,Wang XR,Wei R,Qin K,Lu Y

Published figure using CD11c monoclonal antibody (Product # 46-0116-42) in Flow Cytometry

Wed Jan 20 00:00:00 EST 2021

The long non-coding RNA Cancer Susceptibility 15 (CASC15) is induced by isocitrate dehydrogenase (IDH) mutations and maintains an immature phenotype in adult acute myeloid leukemia.

Haematologica

Grasedieck S,Ruess C,Krowiorz K,Lux S,Pochert N,Schwarzer A,Klusmann JH,Jongen-Lavrencic M,Herold T,Bullinger L,Pollack JR,Rouhi A,Kuchenbauer F

Published figure using CD11c monoclonal antibody (Product # 46-0116-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Human and Mouse Transcriptome Profiling Identifies Cross-Species Homology in Pulmonary and Lymph Node Mononuclear Phagocytes.

Cell reports

Leach SM,Gibbings SL,Tewari AD,Atif SM,Vestal B,Danhorn T,Janssen WJ,Wager TD,Jakubzick CV

Published figure using CD11c monoclonal antibody (Product # 46-0116-42) in Flow Cytometry

Tue Nov 03 00:00:00 EST 2020

Simian-Human Immunodeficiency Virus SHIV.CH505 Infection of Rhesus Macaques Results in Persistent Viral Replication and Induces Intestinal Immunopathology.

Journal of virology

Bar KJ,Coronado E,Hensley-McBain T,O'Connor MA,Osborn JM,Miller C,Gott TM,Wangari S,Iwayama N,Ahrens CY,Smedley J,Moats C,Lynch RM,Haddad EK,Haigwood NL,Fuller DH,Shaw GM,Klatt NR,Manuzak JA

46-0116-42 was used in Flow cytometry/Cell sorting to evaluate the viral dynamics and immunopathology of SHIV.CH505 in rhesus macaques.

Sun Sep 15 00:00:00 EDT 2019

AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.

Oncology letters

Xiu W,Ma J,Lei T,Zhang M

Published figure using CD11c monoclonal antibody (Product # 46-0116-42) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Genetic Architecture of Adaptive Immune System Identifies Key Immune Regulators.

Cell reports

Lagou V,Garcia-Perez JE,Smets I,Van Horebeek L,Vandebergh M,Chen L,Mallants K,Prezzemolo T,Hilven K,Humblet-Baron S,Moisse M,Van Damme P,Boeckxstaens G,Bowness P,Dubois B,Dooley J,Liston A,Goris A

46-0116-42 was used in Flow Cytometry to identify eight genome-wide significant associations explaining 6%-20% of variance in immune system regulation.

Tue Oct 16 00:00:00 EDT 2018

Autophagosome-based strategy to monitor apparent tumor-specific CD8 T cells in patients with prostate cancer.

Oncoimmunology

van de Ven R,Hilton TL,Hu HM,Dubay CJ,Haley D,Paustian C,Puri S,Urba WJ,Curti BD,Aung S,Fox BA

46-0116 was used in Flow cytometry/Cell sorting to study whether a tumour-derived antigen source called DRibbles® can be used to identify tumour-associated antigen-specific responses in patients before or after immunotherapy treatment.

Sat Nov 04 00:00:00 EDT 2023

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using CD11c monoclonal antibody (Product # 46-0116-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Pharmacological targeting of miR-155 via the NEDD8-activating enzyme inhibitor MLN4924 (Pevonedistat) in FLT3-ITD acute myeloid leukemia.

Leukemia

Khalife J,Radomska HS,Santhanam R,Huang X,Neviani P,Saultz J,Wang H,Wu YZ,Alachkar H,Anghelina M,Dorrance A,Curfman J,Bloomfield CD,Medeiros BC,Perrotti D,Lee LJ,Lee RJ,Caligiuri MA,Pichiorri F,Croce CM,Garzon R,Guzman ML,Mendler JH,Marcucci G

Published figure using CD11c monoclonal antibody (Product # 46-0116-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

Published figure using CD11c monoclonal antibody (Product # 46-0116-42) in Flow Cytometry

Tue Sep 15 00:00:00 EDT 2015

Direct contact between dendritic cells and bronchial epithelial cells inhibits T cell recall responses towards mite and pollen allergen extracts in vitro.

Clinical and experimental immunology

Papazian D,Wagtmann VR,Hansen S,Würtzen PA

46-0116-42 was used in Flow Cytometry /test) to conclude that direct contact between DCs and AECs inhibits T cell recall responses towards birch, grass and house dust mite allergens in vitro, suggesting that AECs-DC contact in vivo constitute a key element in mucosal homeostasis in relation to allergic sensitisation.

Sat Aug 01 00:00:00 EDT 2015

Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

The Journal of clinical investigation

Radomska HS,Alberich-Jordà M,Will B,Gonzalez D,Delwel R,Tenen DG

Published figure using CD11c monoclonal antibody (Product # 46-0116-42) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Negative selection by IgM superantigen defines a B cell central tolerance compartment and reveals mutations allowing escape.

Journal of immunology (Baltimore, Md. : 1950)

Duong BH,Ota T,Aoki-Ota M,Cooper AB,Ait-Azzouzene D,Vela JL,Gavin AL,Nemazee D

46-0116 was used in Flow cytometry/Cell sorting to demonstrate the usefulness of reconstituted IgM(b) superantigen hosts in revealing pathways involved in central tolerance.

Thu Dec 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: CD11 antigen-like family member C; CD11c; complement component 3 receptor 4 subunit; integrin alpha X; Integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); Leu M5; leu M5, alpha subunit; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain

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Gene Aliases: CD11C; ITGAX; SLEB6

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UniProt ID: (Human) P20702

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Entrez Gene ID: (Human) 3687

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Function(s)

receptor activity protein binding metal ion binding integrin

Process(es)

cell adhesion integrin-mediated signaling pathway organ morphogenesis extracellular matrix organization heterotypic cell-cell adhesion leukocyte migration positive regulation of protein targeting to mitochondrion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD11c Monoclonal Antibody (3.9), PerCP-eFluor™ 710, eBioscience™

CD11c Antibody (46-0116-42) in Flow

Product Details

46-0116-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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