Invitrogen

CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), APC, eBioscience™

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CD24 Antibody (17-0247-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD24 APC (purple histogram). Cells in the granulocyte gate were used for analysis.

Product Image Gallery

Product Details

17-0247-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 31 publications 31 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioSN3 (SN3 A5-2H10)

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10718833

Product Specific Information

Description: The SN3 monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: This eBioSN3 (SN3 A5-2H10) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioSN3 (SN3 A5-2H10) antibody has been pre-titrated and tested by flow cytometric anaysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Immune Checkpoint Profiling in Humanized Breast Cancer Mice Revealed Cell-Specific LAG-3/PD-1/TIM-3 Co-Expression and Elevated PD-1/TIM-3 Secretion.

Cancers

Bruss C,Kellner K,Albert V,Hutchinson JA,Seitz S,Ortmann O,Brockhoff G,Wege AK

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Thu May 04 00:00:00 EDT 2023

miR‑122/SENP1 axis confers stemness and chemoresistance to liver cancer through Wnt/β‑catenin signaling.

Oncology letters

Dai J,Hao Y,Chen X,Yu Q,Wang B

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Stabilization of E-cadherin adhesions by COX-2/GSK3β signaling is a targetable pathway in metastatic breast cancer.

JCI insight

Balamurugan K,Poria DK,Sehareen SW,Krishnamurthy S,Tang W,McKennett L,Padmanaban V,Czarra K,Ewald AJ,Ueno NT,Ambs S,Sharan S,Sterneck E

17-0247-42 was used in Flow cytometry/Cell sorting to uncover a mechanism by which metastatic breast cancer cells can maintain E-cadherin-mediated cell-cell adhesions and cell survival, suggesting that some patients with COX-2+/E-cadherin+ breast cancer may benefit from targeting of the PGE2 signaling pathway.

Wed Mar 22 00:00:00 EDT 2023

Naringin protects human nucleus pulposus cells against TNF-α-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation.

Oxidative medicine and cellular longevity

Chen R,Gao S,Guan H,Zhang X,Gao Y,Su Y,Song Y,Jiang Y,Li N

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Thu Mar 24 00:00:00 EDT 2022

Elevated transcription and glycosylation of B3GNT5 promotes breast cancer aggressiveness.

Journal of experimental & clinical cancer research : CR

Miao Z,Cao Q,Liao R,Chen X,Li X,Bai L,Ma C,Deng X,Dai Z,Li J,Dong C

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Sat May 07 00:00:00 EDT 2022

Alterations in T and B cell function persist in convalescent COVID-19 patients.

Med (New York, N.Y.)

Shuwa HA,Shaw TN,Knight SB,Wemyss K,McClure FA,Pearmain L,Prise I,Jagger C,Morgan DJ,Khan S,Brand O,Mann ER,Ustianowski A,Bakerly ND,Dark P,Brightling CE,Brij S,Felton T,Simpson A,Grainger JR,Hussell T,Konkel JE,Menon M

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Fri Jun 11 00:00:00 EDT 2021

A CD10-OGP Membrane Peptolytic Signaling Axis in Fibroblasts Regulates Lipid Metabolism of Cancer Stem Cells via SCD1.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Yu S,Lu Y,Su A,Chen J,Li J,Zhou B,Liu X,Xia Q,Li Y,Li J,Huang M,Ye Y,Zhao Q,Jiang S,Yan X,Wang X,Di C,Pan J,Su S

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

IGF2BP2 regulates DANCR by serving as an N6-methyladenosine reader.

Cell death and differentiation

Hu X,Peng WX,Zhou H,Jiang J,Zhou X,Huang D,Mo YY,Yang L

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Chemotherapeutic Stress Influences Epithelial-Mesenchymal Transition and Stemness in Cancer Stem Cells of Triple-Negative Breast Cancer.

International journal of molecular sciences

Li X,Strietz J,Bleilevens A,Stickeler E,Maurer J

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Wed Jan 08 00:00:00 EST 2020

Obesity-Altered Adipose Stem Cells Promote Radiation Resistance of Estrogen Receptor Positive Breast Cancer through Paracrine Signaling.

International journal of molecular sciences

Sabol RA,Villela VA,Denys A,Freeman BT,Hartono AB,Wise RM,Harrison MAA,Sandler MB,Hossain F,Miele L,Bunnell BA

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Wed Apr 15 00:00:00 EDT 2020

Obesity-Altered Adipose Stem Cells Promote Radiation Resistance of Estrogen Receptor Positive Breast Cancer through Paracrine Signaling.

International journal of molecular sciences

Sabol RA,Villela VA,Denys A,Freeman BT,Hartono AB,Wise RM,Harrison MAA,Sandler MB,Hossain F,Miele L,Bunnell BA

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Wed Apr 15 00:00:00 EDT 2020

The Inhibitory Effect of Curcumin on Hypoxia Inducer Factors (Hifs) as a Regulatory Factor in the Growth of Tumor Cells in Breast Cancer Stem-Like Cells.

Drug research

Sarighieh MA,Montazeri V,Shadboorestan A,Ghahremani MH,Ostad SN

17-0247-42 was used in Flow Cytometry to present that curcumin has inhibitory effects on MCF- 7 cells and CS- LCs and thus may be used as an antitumor agent.

Sun Nov 01 00:00:00 EDT 2020

Leptin produced by obesity-altered adipose stem cells promotes metastasis but not tumorigenesis of triple-negative breast cancer in orthotopic xenograft and patient-derived xenograft models.

Breast cancer research : BCR

Sabol RA,Bowles AC,Côté A,Wise R,O'Donnell B,Matossian MD,Hossain FM,Burks HE,Del Valle L,Miele L,Collins-Burow BM,Burow ME,Bunnell BA

17-0247 was used in Flow cytometry/Cell sorting to study the mechanisms by which obesity promotes tumour progression.

Wed May 22 00:00:00 EDT 2019

Integrin α9 depletion promotes β-catenin degradation to suppress triple-negative breast cancer tumor growth and metastasis.

International journal of cancer

Wang Z,Li Y,Xiao Y,Lin HP,Yang P,Humphries B,Gao T,Yang C

17-0247 was used in Flow cytometry/Cell sorting to indicate that ITGA9 depletion suppresses TNBC tumor growth and metastasis by promoting β-catenin degradation through the ILK/PKA/GSK3 pathway.

Fri Nov 15 00:00:00 EST 2019

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

HMGB3 silence inhibits breast cancer cell proliferation and tumor growth by interacting with hypoxia-inducible factor 1α.

Cancer management and research

Gu J,Xu T,Huang QH,Zhang CM,Chen HY

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

BRCA1 regulates the cancer stem cell fate of breast cancer cells in the context of hypoxia and histone deacetylase inhibitors.

Scientific reports

Kim H,Lin Q,Yun Z

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Thu Jul 04 00:00:00 EDT 2019

Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency.

Stem cell reports

Bredenkamp N,Yang J,Clarke J,Stirparo GG,von Meyenn F,Dietmann S,Baker D,Drummond R,Ren Y,Li D,Wu C,Rostovskaya M,Eminli-Meissner S,Smith A,Guo G

17-0247 was used in Flow cytometry/Cell sorting to show induced naive human pluripotent stem cells can be clonally expanded with a diploid karyotype and undergo somatic lineage differentiation following formative transition.

Tue Dec 10 00:00:00 EST 2019

CD22-targeted CAR T cells induce remission in B-ALL that is naive or resistant to CD19-targeted CAR immunotherapy.

Nature medicine

Fry TJ,Shah NN,Orentas RJ,Stetler-Stevenson M,Yuan CM,Ramakrishna S,Wolters P,Martin S,Delbrook C,Yates B,Shalabi H,Fountaine TJ,Shern JF,Majzner RG,Stroncek DF,Sabatino M,Feng Y,Dimitrov DS,Zhang L,Nguyen S,Qin H,Dropulic B,Lee DW,Mackall CL

17-0247 was used in Flow cytometry/Cell sorting to study the role of antigen density in regulating chimeric antigen receptor T cell function.

Mon Jan 01 00:00:00 EST 2018

CD19⁺CD24^hiCD38^hi B Cells Are Expanded in Juvenile Dermatomyositis and Exhibit a Pro-Inflammatory Phenotype After Activation Through Toll-Like Receptor 7 and Interferon-α.

Frontiers in immunology

Piper CJM,Wilkinson MGL,Deakin CT,Otto GW,Dowle S,Duurland CL,Adams S,Marasco E,Rosser EC,Radziszewska A,Carsetti R,Ioannou Y,Beales PL,Kelberman D,Isenberg DA,Mauri C,Nistala K,Wedderburn LR

17-0247 was used in Flow cytometry/Cell sorting to identify that immature transitional B cells were significantly expanded in active JDM, actively dividing, and correlated positively with disease activity.

Wed Nov 20 00:00:00 EST 2019

The hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells.

Breast cancer research : BCR

Kim H,Lin Q,Glazer PM,Yun Z

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Tue Mar 06 00:00:00 EST 2018

Glutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.

Theranostics

Cao Y,Lin SH,Wang Y,Chin YE,Kang L,Mi J

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Mon Apr 30 00:00:00 EDT 2018

HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells.

Molecular cancer

Shah M,Cardenas R,Wang B,Persson J,Mongan NP,Grabowska A,Allegrucci C

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Thu Feb 16 00:00:00 EST 2017

The FOXO3/PGC-1β signaling axis is essential for cancer stem cell properties of pancreatic ductal adenocarcinoma.

The Journal of biological chemistry

Kumazoe M,Takai M,Hiroi S,Takeuchi C,Kadomatsu M,Nojiri T,Onda H,Bae J,Huang Y,Takamatsu K,Yamashita S,Kangawa K,Tachibana H

17-0247 was used in Flow cytometry/Cell sorting to examine the importance of the forkhead box O3/peroxisome proliferator-activated receptor-γ co-activator-1Β signalling axis in CD44 expression and cancer stem cell properties in pancreatic ductal adenocarcinoma.

Fri Jun 30 00:00:00 EDT 2017

Daratumumab depletes CD38+ immune regulatory cells, promotes T-cell expansion, and skews T-cell repertoire in multiple myeloma.

Blood

Krejcik J,Casneuf T,Nijhof IS,Verbist B,Bald J,Plesner T,Syed K,Liu K,van de Donk NW,Weiss BM,Ahmadi T,Lokhorst HM,Mutis T,Sasser AK

17-0247 was used in Flow cytometry/Cell sorting to demonstrate that CD38-expressing immunosuppressive regulatory T and B cells and myeloid-derived suppressor cells are sensitive to daratumumab treatment.

Thu Jul 21 00:00:00 EDT 2016

The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states.

BMC biology

Toneff MJ,Sreekumar A,Tinnirello A,Hollander PD,Habib S,Li S,Ellis MJ,Xin L,Mani SA,Rosen JM

17-0247 was used in Flow cytometry/Cell sorting to directly observe epithelial-mesenchymal and mesenchymal-epithelial transition in breast cancer cells using a stably integrating dual sensor system.

Fri Jun 17 00:00:00 EDT 2016

Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.

Oncotarget

Greenwood E,Maisel S,Ebertz D,Russ A,Pandey R,Schroeder J

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Tue Sep 20 00:00:00 EDT 2016

TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis.

Oncogene

Cyr AR,Kulak MV,Park JM,Bogachek MV,Spanheimer PM,Woodfield GW,White-Baer LS,O'Malley YQ,Sugg SL,Olivier AK,Zhang W,Domann FE,Weigel RJ

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Thu Jan 22 00:00:00 EST 2015

Special AT-rich sequence-binding protein-1 participates in the maintenance of breast cancer stem cells through regulation of the Notch signaling pathway and expression of Snail1 and Twist1.

Molecular medicine reports

Sun Z,Zhang C,Zou X,Jiang G,Xu Z,Li W,Xie H

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Fri May 01 00:00:00 EDT 2015

NDY1/KDM2B functions as a master regulator of polycomb complexes and controls self-renewal of breast cancer stem cells.

Cancer research

Kottakis F,Foltopoulou P,Sanidas I,Keller P,Wronski A,Dake BT,Ezell SA,Shen Z,Naber SP,Hinds PW,McNiel E,Kuperwasser C,Tsichlis PN

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Tue Jul 15 00:00:00 EDT 2014

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

17-0247 was used in Flow cytometry/Cell sorting to study how the GATA3-miR-29b axis regulates the tumour microenvironment, and inhibits metastasis to open up possibilities for therapeutic intervention in breast cancer.

Fri Feb 01 00:00:00 EST 2013

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Fri Feb 01 00:00:00 EST 2013

Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways.

Breast cancer research : BCR

Iwanaga R,Wang CA,Micalizzi DS,Harrell JC,Jedlicka P,Sartorius CA,Kabos P,Farabaugh SM,Bradford AP,Ford HL

Published figure using CD24 monoclonal antibody (Product # 17-0247-42) in Flow Cytometry

Thu Jul 05 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); FLJ22950; FLJ43543; MGC75043; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen

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Gene Aliases: CD24; CD24A

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UniProt ID: (Human) P25063

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Entrez Gene ID: (Human) 100133941

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Function(s)

signal transducer activity protein binding protein kinase binding protein tyrosine kinase activator activity

Process(es)

response to hypoxia cell activation regulation of cytokine-mediated signaling pathway response to molecule of bacterial origin immune response-regulating cell surface receptor signaling pathway positive regulation of cytosolic calcium ion concentration Wnt signaling pathway single organismal cell-cell adhesion cell migration regulation of epithelial cell differentiation T cell costimulation B cell receptor transport into membrane raft chemokine receptor transport out of membrane raft negative regulation of transforming growth factor beta3 production positive regulation of activated T cell proliferation regulation of phosphorylation cholesterol homeostasis positive regulation of MAP kinase activity regulation of MAPK cascade response to estrogen respiratory burst positive regulation of protein tyrosine kinase activity glomerular visceral epithelial cell differentiation glomerular parietal epithelial cell differentiation intrinsic apoptotic signaling pathway positive regulation of nephron tubule epithelial cell differentiation

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CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), APC, eBioscience™

CD24 Antibody (17-0247-42) in Flow

Product Details

17-0247-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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