Invitrogen

CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), PE-Cyanine7, eBioscience™

FIGURE: 1 / 31

CD24 Antibody (25-0247-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD24 PE-Cyanine7 (purple histogram). Cells in the granulocyte gate were used for analysis.

Product Image Gallery

Product Details

25-0247-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioSN3 (SN3 A5-2H10)

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573334

Product Specific Information

Description: The eBioSN3 (SN3 A5-2H10) monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HAS). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: This eBioSN3 (SN3 A5-2H10) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioSN3 (SN3 A5-2H10) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Immune Checkpoint Profiling in Humanized Breast Cancer Mice Revealed Cell-Specific LAG-3/PD-1/TIM-3 Co-Expression and Elevated PD-1/TIM-3 Secretion.

Cancers

Bruss C,Kellner K,Albert V,Hutchinson JA,Seitz S,Ortmann O,Brockhoff G,Wege AK

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Thu May 04 00:00:00 EDT 2023

miR‑122/SENP1 axis confers stemness and chemoresistance to liver cancer through Wnt/β‑catenin signaling.

Oncology letters

Dai J,Hao Y,Chen X,Yu Q,Wang B

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Naringin protects human nucleus pulposus cells against TNF-α-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation.

Oxidative medicine and cellular longevity

Chen R,Gao S,Guan H,Zhang X,Gao Y,Su Y,Song Y,Jiang Y,Li N

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Thu Mar 24 00:00:00 EDT 2022

Elevated transcription and glycosylation of B3GNT5 promotes breast cancer aggressiveness.

Journal of experimental & clinical cancer research : CR

Miao Z,Cao Q,Liao R,Chen X,Li X,Bai L,Ma C,Deng X,Dai Z,Li J,Dong C

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Sat May 07 00:00:00 EDT 2022

Alterations in T and B cell function persist in convalescent COVID-19 patients.

Med (New York, N.Y.)

Shuwa HA,Shaw TN,Knight SB,Wemyss K,McClure FA,Pearmain L,Prise I,Jagger C,Morgan DJ,Khan S,Brand O,Mann ER,Ustianowski A,Bakerly ND,Dark P,Brightling CE,Brij S,Felton T,Simpson A,Grainger JR,Hussell T,Konkel JE,Menon M

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Fri Jun 11 00:00:00 EDT 2021

A CD10-OGP Membrane Peptolytic Signaling Axis in Fibroblasts Regulates Lipid Metabolism of Cancer Stem Cells via SCD1.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Yu S,Lu Y,Su A,Chen J,Li J,Zhou B,Liu X,Xia Q,Li Y,Li J,Huang M,Ye Y,Zhao Q,Jiang S,Yan X,Wang X,Di C,Pan J,Su S

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

IGF2BP2 regulates DANCR by serving as an N6-methyladenosine reader.

Cell death and differentiation

Hu X,Peng WX,Zhou H,Jiang J,Zhou X,Huang D,Mo YY,Yang L

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Chemotherapeutic Stress Influences Epithelial-Mesenchymal Transition and Stemness in Cancer Stem Cells of Triple-Negative Breast Cancer.

International journal of molecular sciences

Li X,Strietz J,Bleilevens A,Stickeler E,Maurer J

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Wed Jan 08 00:00:00 EST 2020

Obesity-Altered Adipose Stem Cells Promote Radiation Resistance of Estrogen Receptor Positive Breast Cancer through Paracrine Signaling.

International journal of molecular sciences

Sabol RA,Villela VA,Denys A,Freeman BT,Hartono AB,Wise RM,Harrison MAA,Sandler MB,Hossain F,Miele L,Bunnell BA

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Wed Apr 15 00:00:00 EDT 2020

HMGB3 silence inhibits breast cancer cell proliferation and tumor growth by interacting with hypoxia-inducible factor 1α.

Cancer management and research

Gu J,Xu T,Huang QH,Zhang CM,Chen HY

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

BRCA1 regulates the cancer stem cell fate of breast cancer cells in the context of hypoxia and histone deacetylase inhibitors.

Scientific reports

Kim H,Lin Q,Yun Z

25-0247-42 was used in Flow cytometry/Cell sorting to examine the role of the tumor suppressor BRCA1 and hypoxia in the regulation of cancer cell stemness using genetically matched breast cancer cell lines.

Thu Jul 04 00:00:00 EDT 2019

BRCA1 regulates the cancer stem cell fate of breast cancer cells in the context of hypoxia and histone deacetylase inhibitors.

Scientific reports

Kim H,Lin Q,Yun Z

Thu Jul 04 00:00:00 EDT 2019

The hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells.

Breast cancer research : BCR

Kim H,Lin Q,Glazer PM,Yun Z

25-0247 was used in Flow cytometry/Cell sorting to suggest that the hypoxic TME promotes malignant progression and therapy resistance via induction, selection and preferential maintenance of CSC-like cells.

Tue Mar 06 00:00:00 EST 2018

The hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells.

Breast cancer research : BCR

Kim H,Lin Q,Glazer PM,Yun Z

Tue Mar 06 00:00:00 EST 2018

Glutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.

Theranostics

Cao Y,Lin SH,Wang Y,Chin YE,Kang L,Mi J

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Mon Apr 30 00:00:00 EDT 2018

HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells.

Molecular cancer

Shah M,Cardenas R,Wang B,Persson J,Mongan NP,Grabowska A,Allegrucci C

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Thu Feb 16 00:00:00 EST 2017

The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states.

BMC biology

Toneff MJ,Sreekumar A,Tinnirello A,Hollander PD,Habib S,Li S,Ellis MJ,Xin L,Mani SA,Rosen JM

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Fri Jun 17 00:00:00 EDT 2016

Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.

Oncotarget

Greenwood E,Maisel S,Ebertz D,Russ A,Pandey R,Schroeder J

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Tue Sep 20 00:00:00 EDT 2016

PD-L1 mediated the differentiation of tumor-infiltrating CD19⁺ B lymphocytes and T cells in Invasive breast cancer.

Oncoimmunology

Guan H,Lan Y,Wan Y,Wang Q,Wang C,Xu L,Chen Y,Liu W,Zhang X,Li Y,Gu Y,Wang Z,Xie F

25-0247 was used in Flow cytometry/Cell sorting to investigate the role of B cells in human breast cancer etiology.

Mon Feb 01 00:00:00 EST 2016

TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis.

Oncogene

Cyr AR,Kulak MV,Park JM,Bogachek MV,Spanheimer PM,Woodfield GW,White-Baer LS,O'Malley YQ,Sugg SL,Olivier AK,Zhang W,Domann FE,Weigel RJ

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Thu Jan 22 00:00:00 EST 2015

Special AT-rich sequence-binding protein-1 participates in the maintenance of breast cancer stem cells through regulation of the Notch signaling pathway and expression of Snail1 and Twist1.

Molecular medicine reports

Sun Z,Zhang C,Zou X,Jiang G,Xu Z,Li W,Xie H

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Fri May 01 00:00:00 EDT 2015

NDY1/KDM2B functions as a master regulator of polycomb complexes and controls self-renewal of breast cancer stem cells.

Cancer research

Kottakis F,Foltopoulou P,Sanidas I,Keller P,Wronski A,Dake BT,Ezell SA,Shen Z,Naber SP,Hinds PW,McNiel E,Kuperwasser C,Tsichlis PN

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Tue Jul 15 00:00:00 EDT 2014

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways.

Breast cancer research : BCR

Iwanaga R,Wang CA,Micalizzi DS,Harrell JC,Jedlicka P,Sartorius CA,Kabos P,Farabaugh SM,Bradford AP,Ford HL

Published figure using CD24 monoclonal antibody (Product # 25-0247-42) in Flow Cytometry

Thu Jul 05 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); FLJ22950; FLJ43543; MGC75043; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen

View more View less

Gene Aliases: CD24; CD24A

View more View less

UniProt ID: (Human) P25063

View more View less

Entrez Gene ID: (Human) 100133941

View more View less

Function(s)

signal transducer activity protein binding protein kinase binding protein tyrosine kinase activator activity

Process(es)

response to hypoxia cell activation regulation of cytokine-mediated signaling pathway response to molecule of bacterial origin immune response-regulating cell surface receptor signaling pathway positive regulation of cytosolic calcium ion concentration Wnt signaling pathway single organismal cell-cell adhesion cell migration regulation of epithelial cell differentiation T cell costimulation B cell receptor transport into membrane raft chemokine receptor transport out of membrane raft negative regulation of transforming growth factor beta3 production positive regulation of activated T cell proliferation regulation of phosphorylation cholesterol homeostasis positive regulation of MAP kinase activity regulation of MAPK cascade response to estrogen respiratory burst positive regulation of protein tyrosine kinase activity glomerular visceral epithelial cell differentiation glomerular parietal epithelial cell differentiation intrinsic apoptotic signaling pathway positive regulation of nephron tubule epithelial cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), PE-Cyanine7, eBioscience™

CD24 Antibody (25-0247-42) in Flow

Product Details

25-0247-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!