Invitrogen

CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), APC, eBioscience™

CD62L (L-Selectin) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

CD62L (L-Selectin) Antibody (17-0629-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0048-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD62L (L-Selectin) APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-0629-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 20 publications 20 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DREG-56 (DREG56)

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10671132

Product Specific Information

Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported: The DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis.

Applications Tested: The DREG-56 (DREG56) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Induction of memory-like CD8+ T cells and CD4+ T cells from human naive T cells in culture.

Clinical and experimental immunology

Tokumoto Y,Araki Y,Narizuka Y,Mizuno Y,Ohshima S,Mimura T

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 17-0629-42) in Flow Cytometry

Fri Jan 28 00:00:00 EST 2022

Immune composition and its association with hematologic recovery after chemotherapeutic injury in acute myeloid leukemia.

Experimental hematology

Kenswil KJG,Pisterzi P,Feyen J,Ter Borg M,Rombouts E,Braakman E,Raaijmakers MHGP

17-0629-42 was used in Flow cytometry/Cell sorting to provide novel insights into adaptive immune cell recovery after injury and identify the NK/CD4CM index as a putative predictor of hematopoietic recovery in AML.

Sat Jan 01 00:00:00 EST 2022

Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.

International journal of inflammation

Genkel V,Dolgushin I,Baturina I,Savochkina A,Kuznetsova A,Pykhova L,Shaposhnik I

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 17-0629-42) in Flow Cytometry

Sun Apr 24 00:00:00 EDT 2022

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 17-0629-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.

Nature communications

Yang C,Siebert JR,Burns R,Gerbec ZJ,Bonacci B,Rymaszewski A,Rau M,Riese MJ,Rao S,Carlson KS,Routes JM,Verbsky JW,Thakar MS,Malarkannan S

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 17-0629-42) in Flow Cytometry

Mon Sep 02 00:00:00 EDT 2019

IL-17 inhibits CXCL9/10-mediated recruitment of CD8⁺ cytotoxic T cells and regulatory T cells to colorectal tumors.

Journal for immunotherapy of cancer

Chen J,Ye X,Pitmon E,Lu M,Wan J,Jellison ER,Adler AJ,Vella AT,Wang K

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 17-0629-42) in Flow Cytometry

Wed Nov 27 00:00:00 EST 2019

n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Molecular medicine reports

Wu B,Wang L,Jiang L,Dong L,Xu F,Lu Y,Jin J,Wang Z,Liang G,Shan X

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 17-0629-42) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

17-0629 was used in Flow cytometry/Cell sorting to demonstrate that blocking PITPNM3 recognition of CCL18 could be an attractive immunotherapy aproach for treating breast cancer.

Sat Apr 01 00:00:00 EDT 2017

Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.

The Journal of clinical investigation

Rathod KS,Kapil V,Velmurugan S,Khambata RS,Siddique U,Khan S,Van Eijl S,Gee LC,Bansal J,Pitrola K,Shaw C,D'Acquisto F,Colas RA,Marelli-Berg F,Dalli J,Ahluwalia A

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 17-0629-42) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2017

Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.

Nature communications

Douam F,Hrebikova G,Albrecht YE,Sellau J,Sharon Y,Ding Q,Ploss A

17-0629 was used in Flow cytometry/Cell sorting to demonstrate the tracking of and sense viral RNA at single-cell resolution within complex subsets of the human and murine immune system in different mouse models.

Tue Mar 14 00:00:00 EDT 2017

Type III Interferon-Mediated Signaling Is Critical for Controlling Live Attenuated Yellow Fever Virus Infection In Vivo.

mBio

Douam F,Soto Albrecht YE,Hrebikova G,Sadimin E,Davidson C,Kotenko SV,Ploss A

17-0629 was used in Flow cytometry/Cell sorting to analyse the role of type III interferon (IFN)-mediated signaling in controlling the infection and attenuation of the live attenuated yellow virus strain, YFV-17D.

Tue Aug 15 00:00:00 EDT 2017

Blocking MHC class II on human endothelium mitigates acute rejection.

JCI insight

Abrahimi P,Qin L,Chang WG,Bothwell AL,Tellides G,Saltzman WM,Pober JS

17-0629 was used in Flow cytometry/Cell sorting to establish the immune cell network involved in acute allograft rejection in humans and identify targets for minimising this rejection.

Wed Nov 20 00:00:00 EST 2019

The CD14+CD16+ inflammatory monocyte subset displays increased mitochondrial activity and effector function during acute Plasmodium vivax malaria.

PLoS pathogens

Antonelli LR,Leoratti FM,Costa PA,Rocha BC,Diniz SQ,Tada MS,Pereira DB,Teixeira-Carvalho A,Golenbock DT,Gonçalves R,Gazzinelli RT

17-0629 was used in Flow cytometry/Cell sorting to study inflammatory monocyte subsets displaying increased mitochondrial activity and effector function during malaria.

Mon Sep 01 00:00:00 EDT 2014

Immune responses induced by T-cell vaccination in patients with rheumatoid arthritis.

Human vaccines & immunotherapeutics

Ivanova I,Seledtsova G,Mamaev S,Shishkov A,Seledtsov V

17-0629 was used in Flow cytometry/Cell sorting to study how the T-cell immunotherapy regimen used resulted in the clinical improvement, which was achieved in 87% patients.

Mon Jun 22 00:00:00 EDT 2015

Neutrophil paralysis in Plasmodium vivax malaria.

PLoS neglected tropical diseases

Leoratti FM,Trevelin SC,Cunha FQ,Rocha BC,Costa PA,Gravina HD,Tada MS,Pereira DB,Golenbock DT,Antonelli LR,Gazzinelli RT

17-0629 was used in Flow cytometry/Cell sorting to investigate the activation and function of neutrophils during acute episodes of Plasmodium vivax malaria.

Fri Sep 28 00:00:00 EDT 2012

Matrix metalloproteinase-2 conditions human dendritic cells to prime inflammatory T(H)2 cells via an IL-12- and OX40L-dependent pathway.

Cancer cell

Godefroy E,Manches O,Dréno B,Hochman T,Rolnitzky L,Labarrière N,Guilloux Y,Goldberg J,Jotereau F,Bhardwaj N

17-0629 was used in Flow cytometry/Cell sorting to reveal that MMP2-conditioned DCs prime Th2 responses against several melanoma-associated antigens, highlighting the potential of MMP2 as a target antigen in melanoma.

Tue Mar 08 00:00:00 EST 2011

Differential expression of CD300a/c on human TH1 and TH17 cells.

BMC immunology

Simhadri VR,Mariano JL,Zhou Q,DeBell KE,Borrego F

17-0629 was used in Flow cytometry/Cell sorting to conclude that the CD300a/c receptors are differentially expressed on human TH1 and TH17 cells, and that their ligation is capable of modulating TCR mediated signals.

Wed Nov 02 00:00:00 EDT 2011

Monocyte surface expression of Fcgamma receptor RI (CD64), a biomarker reflecting type-I interferon levels in systemic lupus erythematosus.

Arthritis research & therapy

Li Y,Lee PY,Kellner ES,Paulus M,Switanek J,Xu Y,Zhuang H,Sobel ES,Segal MS,Satoh M,Reeves WH

17-0629 was used in Flow cytometry/Cell sorting to identify a surrogate marker for IFN-I activity in clinical samples for monitoring lupus activity and response to therapy.

Wed Nov 24 00:00:00 EST 2010

Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.

PloS one

Narayanan S,Silva R,Peruzzi G,Alvarez Y,Simhadri VR,Debell K,Coligan JE,Borrego F

17-0629 was used in Flow cytometry/Cell sorting to investigate a subset within the CD4 T cell population defined by expression of the immunomodulatory receptor CD300a.

Thu May 13 00:00:00 EDT 2010

Increased expression of FcgammaRI/CD64 on circulating monocytes parallels ongoing inflammation and nephritis in lupus.

Arthritis research & therapy

Li Y,Lee PY,Sobel ES,Narain S,Satoh M,Segal MS,Reeves WH,Richards HB

17-0629 was used in Flow cytometry/Cell sorting to measure surface expression of Fc-gamma receptors on circulating monocytes from systemic lupus erythematosus patients.

Wed Sep 09 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD62 antigen-like family member L; CD62L; gp90-MEL; hLHRc; L-selectin; LAM-1; LECAM1; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; TQ1

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Gene Aliases: CD62L; LAM1; LECAM1; LEU8; LNHR; LSEL; LYAM1; PLNHR; SELL; TQ1

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UniProt ID: (Human) P14151

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Entrez Gene ID: (Human) 6402

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Function(s)

protease binding protein binding heparin binding carbohydrate binding glycosphingolipid binding cell adhesion molecule binding

Process(es)

cell adhesion response to ATP regulation of immune response leukocyte migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), APC, eBioscience™

CD62L (L-Selectin) Antibody (17-0629-42) in Flow

Product Details

17-0629-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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