Invitrogen

CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), PE-Cyanine7, eBioscience™

CD62L (L-Selectin) Antibody in Flow Cytometry (Flow)

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CD62L (L-Selectin) Antibody (25-0629-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0048-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD62L (L-Seletin) PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

25-0629-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 20 publications 20 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DREG-56 (DREG56)

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1257142

Product Specific Information

Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported: This DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DREG-56 (DREG56) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Induction of memory-like CD8+ T cells and CD4+ T cells from human naive T cells in culture.

Clinical and experimental immunology

Tokumoto Y,Araki Y,Narizuka Y,Mizuno Y,Ohshima S,Mimura T

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 25-0629-42) in Flow Cytometry

Fri Jan 28 00:00:00 EST 2022

Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.

International journal of inflammation

Genkel V,Dolgushin I,Baturina I,Savochkina A,Kuznetsova A,Pykhova L,Shaposhnik I

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 25-0629-42) in Flow Cytometry

Sun Apr 24 00:00:00 EDT 2022

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 25-0629-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.

Nature communications

Yang C,Siebert JR,Burns R,Gerbec ZJ,Bonacci B,Rymaszewski A,Rau M,Riese MJ,Rao S,Carlson KS,Routes JM,Verbsky JW,Thakar MS,Malarkannan S

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 25-0629-42) in Flow Cytometry

Mon Sep 02 00:00:00 EDT 2019

TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.

eLife

Ma X,Yang T,Luo Y,Wu L,Jiang Y,Song Z,Pan T,Liu B,Liu G,Liu J,Yu F,He Z,Zhang W,Yang J,Liang L,Guan Y,Zhang X,Li L,Cai W,Tang X,Gao S,Deng K,Zhang H

25-0629-42 was used in Flow Cytometry to show that TRIM28 potently suppresses HIV-1 expression by utilizing both SUMO E3 ligase activity and epigenetic adaptor function.

Thu Jan 17 00:00:00 EST 2019

Outcomes of controlled human malaria infection after BCG vaccination.

Nature communications

Walk J,de Bree LCJ,Graumans W,Stoter R,van Gemert GJ,van de Vegte-Bolmer M,Teelen K,Hermsen CC,Arts RJW,Behet MC,Keramati F,Moorlag SJCFM,Yang ASP,van Crevel R,Aaby P,de Mast Q,van der Ven AJAM,Stabell Benn C,Netea MG,Sauerwein RW

25-0629 was used in Flow cytometry/Cell sorting to evaluate the induction of immunity and the protective efficacy of the anti-tuberculosis Bacillus-Calmette Guerin vaccine against a controlled human malaria infection.

Wed Feb 20 00:00:00 EST 2019

n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Molecular medicine reports

Wu B,Wang L,Jiang L,Dong L,Xu F,Lu Y,Jin J,Wang Z,Liang G,Shan X

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 25-0629-42) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.

The Journal of clinical investigation

Rathod KS,Kapil V,Velmurugan S,Khambata RS,Siddique U,Khan S,Van Eijl S,Gee LC,Bansal J,Pitrola K,Shaw C,D'Acquisto F,Colas RA,Marelli-Berg F,Dalli J,Ahluwalia A

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 25-0629-42) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2017

Modulation of Endoplasmic Reticulum Stress Controls CD4⁺ T-cell Activation and Antitumor Function.

Cancer immunology research

Thaxton JE,Wallace C,Riesenberg B,Zhang Y,Paulos CM,Beeson CC,Liu B,Li Z

25-0629 was used in Flow cytometry/Cell sorting to investigate whether the endoplasmic reticulum stress response may be important for CD4+ T-cell activation and whether gp96 is integral to this process.

Tue Aug 01 00:00:00 EDT 2017

Functional T cells targeting tumor-associated antigens are predictive for recurrence-free survival of patients with radically operated non-small cell lung cancer.

Oncoimmunology

Safi S,Yamauchi Y,Rathinasamy A,Stamova S,Eichhorn M,Warth A,Rauch G,Dienemann H,Hoffmann H,Beckhove P

25-0629 was used in Flow cytometry/Cell sorting to evaluate postoperative follow up and preoperative IFN-y T cell responses against 14non-small cell lung cancer antigens.

Tue Jan 12 00:00:00 EST 2021

Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand.

PLoS pathogens

Tomić A,Varanasi PR,Golemac M,Malić S,Riese P,Borst EM,Mischak-Weissinger E,Guzmán CA,Krmpotić A,Jonjić S,Messerle M

25-0629 was used in Flow cytometry/Cell sorting to investigate a strategy for an HCMV vaccine that aims at the simultaneous activation of innate and adaptive immune responses.

Thu Dec 01 00:00:00 EST 2016

Immunodominant Dengue Virus-Specific CD8+ T Cell Responses Are Associated with a Memory PD-1+ Phenotype.

Journal of virology

de Alwis R,Bangs DJ,Angelo MA,Cerpas C,Fernando A,Sidney J,Peters B,Gresh L,Balmaseda A,de Silva AD,Harris E,Sette A,Weiskopf D

25-0629 was used in Flow cytometry/Cell sorting to define DENV-specific CD8(+)T cell responses in general and those of HLA alleles associated with dominant responses.

Sun May 01 00:00:00 EDT 2016

PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer.

Cancer immunology research

Severson JJ,Serracino HS,Mateescu V,Raeburn CD,McIntyre RC,Sams SB,Haugen BR,French JD

25-0629 was used in Flow cytometry/Cell sorting to determine that the release of PD-1 and Tim-3, and the inhibition of Tregs, may encourage tumour elimination in metastatic differentiated thyroid cancer.

Mon Jun 01 00:00:00 EDT 2015

Efficient and reproducible generation of tumour-infiltrating lymphocytes for renal cell carcinoma.

British journal of cancer

Baldan V,Griffiths R,Hawkins RE,Gilham DE

25-0629 was used in Flow cytometry/Cell sorting to show tumour-infiltrating lymphocytes can be consistently produced from renal cell carcinoma biopsies maintaining autologous tumour recognition after expansion in vitro.

Tue Apr 28 00:00:00 EDT 2015

Impact of malaria preexposure on antiparasite cellular and humoral immune responses after controlled human malaria infection.

Infection and immunity

Obiero JM,Shekalaghe S,Hermsen CC,Mpina M,Bijker EM,Roestenberg M,Teelen K,Billingsley PF,Sim BK,James ER,Daubenberger CA,Hoffman SL,Abdulla S,Sauerwein RW,Scholzen A

25-0629 was used in Flow cytometry/Cell sorting to show positive P. falciparum lysate serology can be used to identify individuals with better parasite control but weaker IFN-γ responses in circulating lymphocytes.

Fri May 01 00:00:00 EDT 2015

Dendritic cell-MHC class II and Itk regulate functional development of regulatory innate memory CD4+ T cells in bone marrow transplantation.

Journal of immunology (Baltimore, Md. : 1950)

Huang W,Qi Q,Hu J,Huang F,Laufer TM,August A

25-0629 was used in Flow cytometry/Cell sorting to demonstrate that dendritic cells-MHCII and IL-2-inducible T cell kinase regulate the functional development of IMP CD4(+) T cells.

Tue Apr 01 00:00:00 EDT 2014

Proportions of CD4+ memory T cells are altered in individuals chronically infected with Schistosoma haematobium.

Scientific reports

Nausch N,Bourke CD,Appleby LJ,Rujeni N,Lantz O,Trottein F,Midzi N,Mduluza T,Mutapi F

25-0629 was used in Flow cytometry/Cell sorting to investigate the association between human helminth infection and CD4+ memory T cell proportion in individuals chronically infected with Schistosoma haematobium.

Mon Feb 25 00:00:00 EST 2013

Longevity and composition of cellular immune responses following experimental Plasmodium falciparum malaria infection in humans.

PLoS pathogens

Teirlinck AC,McCall MB,Roestenberg M,Scholzen A,Woestenenk R,de Mast Q,van der Ven AJ,Hermsen CC,Luty AJ,Sauerwein RW

25-0629 was used in Flow cytometry/Cell sorting to study cellular immunological responses to sporozoites and asexual blood-stage malaria parasites.

Thu Dec 01 00:00:00 EST 2011

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

25-0629 was used in Flow cytometry/Cell sorting to study the effects of Foxp3 and IL-2 on FCRL3-/+ nTreg cell subsets.

Mon Jun 15 00:00:00 EDT 2009

Vaccines based on novel adeno-associated virus vectors elicit aberrant CD8+ T-cell responses in mice.

Journal of virology

Lin J,Zhi Y,Mays L,Wilson JM

25-0629 was used in Flow cytometry/Cell sorting to evaluate the potential of vectors based on several novel AAVs as vaccine carriers for HIV Gag.

Thu Nov 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD62 antigen-like family member L; CD62L; gp90-MEL; hLHRc; L-selectin; LAM-1; LECAM1; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; TQ1

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Gene Aliases: CD62L; LAM1; LECAM1; LEU8; LNHR; LSEL; LYAM1; PLNHR; SELL; TQ1

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UniProt ID: (Human) P14151

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Entrez Gene ID: (Human) 6402

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Function(s)

protease binding protein binding heparin binding carbohydrate binding glycosphingolipid binding cell adhesion molecule binding

Process(es)

cell adhesion response to ATP regulation of immune response leukocyte migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), PE-Cyanine7, eBioscience™

CD62L (L-Selectin) Antibody (25-0629-42) in Flow

Product Details

25-0629-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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