Invitrogen

ROR gamma (t) Monoclonal Antibody (B2D), APC, eBioscience™

ROR gamma (t) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

ROR gamma (t) Antibody (17-6981-82) in Flow

Intracellular staining of C57Bl/6 splenocytes with Anti-Mouse CD4 APC-eFluor® 780 (Product # 47-0041-82) and 0.5 µg of Rat IgG1 K Isotype Control APC (Product # 17-4301-82) (left) or 0.5 µg of Anti-Mouse ROR gamma (t) APC (right) using the Foxp3 Staining Buffer Set (Product # 00-5523-00). Cells were also stained with Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), Anti-Mouse CD19 FITC (Product # 11-0193-82) and Anti-Mouse CD3 PerCP-eFluor® 710 (Product # 46-0032-82). Viable, CD3. CD19. cells were used for analysis.

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Product Details

17-6981-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 40 publications 40 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B2D

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573254

Product Specific Information

Description: The B2D monoclonal antibody reacts with the mouse ROR gamma (t) protein. ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma t, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.

The ROR gamma t isoform differs from ROR gamma by three unique amino acids at the amino terminus. Therefore, the B2D antibody will react with both the ROR gamma and ROR gamma t isoforms.

Applications Reported: This B2D antibody has been reported for use in flow cytometric analysis.

Applications Tested: This B2D antibody has been tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.

ROR gammat t (RORgt) is an isoform of RORC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A novel interleukin-2-based fusion molecule, HCW9302, differentially promotes regulatory T cell expansion to treat atherosclerosis in mice.

Frontiers in immunology

Zhu X,Li Q,George V,Spanoudis C,Gilkes C,Shrestha N,Liu B,Kong L,You L,Echeverri C,Li L,Wang Z,Chaturvedi P,Muniz GJ,Egan JO,Rhode PR,Wong HC

17-6981-82 was used in flow cytometry to find that HCW9302 exhibited a longer serum half-life with an approximately 1000-fold higher affinity for the IL-2Rα than IL-2.

Mon Feb 13 00:00:00 EST 2023

Maternal gut bacteria drive intestinal inflammation in offspring with neurodevelopmental disorders by altering the chromatin landscape of CD4⁺ T cells.

Immunity

Kim E,Paik D,Ramirez RN,Biggs DG,Park Y,Kwon HK,Choi GB,Huh JR

17-6981-82 was used in Flow cytometry/Cell sorting to investigate intestinal inflammation in children with autism spectrum disorders in response to maternal gut bacteria.

Tue Jan 11 00:00:00 EST 2022

DOCK8-expressing T follicular helper cells newly generated beyond self-organized criticality cause systemic lupus erythematosus.

iScience

Shiozawa S,Tsumiyama K,Miyazaki Y,Uto K,Sakurai K,Nakashima T,Matsuyama H,Doi A,Tarui M,Izumikawa M,Kimura M,Fujita Y,Satonaka C,Horiuchi T,Matsubara T,Oribe M,Yamane T,Kagawa H,Li QZ,Mizuno K,Mukai Y,Murakami K,Enya T,Tsukimoto S,Hakata Y,Miyazawa M,Shiozawa K

17-6981-82 was used in Flow Cytometry to study the induction of systemic autoimmunity in mice normally not prone to autoimmune disease by repeated stimulation with an antigen.

Fri Jan 21 00:00:00 EST 2022

Glycolytic ATP fuels phosphoinositide 3-kinase signaling to support effector T helper 17 cell responses.

Immunity

Xu K,Yin N,Peng M,Stamatiades EG,Chhangawala S,Shyu A,Li P,Zhang X,Do MH,Capistrano KJ,Chou C,Leslie CS,Li MO

17-6981-82 was used in Flow cytometry/Cell sorting to show that Warburg metabolism augments glycolytic ATP production, fueling a PI3K-centered positive feedback regulatory circuit that drives effector T cell responses.

Tue May 11 00:00:00 EDT 2021

ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation.

Cellular & molecular immunology

Liu Y,Bockermann R,Hadi M,Safari I,Carrion B,Kveiborg M,Issazadeh-Navikas S

17-6981-82 was used in Flow Cytometry to study the role of ADAM12 in T-cell responses, fate determination on activation, and its functions in T cells to mediate tissue inflammation.

Sun Aug 01 00:00:00 EDT 2021

IL-1β-driven osteoclastogenic Tregs accelerate bone erosion in arthritis.

The Journal of clinical investigation

Levescot A,Chang MH,Schnell J,Nelson-Maney N,Yan J,Martínez-Bonet M,Grieshaber-Bouyer R,Lee PY,Wei K,Blaustein RB,Morris A,Wactor A,Iwakura Y,Lederer JA,Rao DA,Charles JF,Nigrovic PA

17-6981-82 was used in Flow Cytometry to identify IL-1β-induced osteoclastogenic Tregs as a contributor to bone erosion in arthritis.

Wed Sep 15 00:00:00 EDT 2021

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

17-6981-82 was used in Flow Cytometry to show a persistent accumulation of T cells in mice and human autopsy samples for more than 1 mo after stroke.

Mon Aug 02 00:00:00 EDT 2021

Francisella tularensis induces Th1 like MAIT cells conferring protection against systemic and local infection.

Nature communications

Zhao Z,Wang H,Shi M,Zhu T,Pediongco T,Lim XY,Meehan BS,Nelson AG,Fairlie DP,Mak JYW,Eckle SBG,de Lima Moreira M,Tumpach C,Bramhall M,Williams CG,Lee HJ,Haque A,Evrard M,Rossjohn J,McCluskey J,Corbett AJ,Chen Z

17-6981-82 was used in Flow Cytometry to highlight the potential utility of targeting MAIT cells to combat a range of bacterial pathogens.

Fri Jul 16 00:00:00 EDT 2021

Redundant cytokine requirement for intestinal microbiota-induced Th17 cell differentiation in draining lymph nodes.

Cell reports

Sano T,Kageyama T,Fang V,Kedmi R,Martinez CS,Talbot J,Chen A,Cabrera I,Gorshko O,Kurakake R,Yang Y,Ng C,Schwab SR,Littman DR

17-6981-82 was used in Flow Cytometry to show that intestine-draining mesenteric lymph nodes (MLNs), not intestine proper, are the dominant site of SFB-induced intestinal Th17 cell differentiation.

Tue Aug 24 00:00:00 EDT 2021

Retinoic Acid Induces Functionally Suppressive Foxp3⁺RORγt⁺ T Cells In Vitro.

Frontiers in immunology

Martínez-Blanco M,Lozano-Ojalvo D,Pérez-Rodríguez L,Benedé S,Molina E,López-Fandiño R

17-6981-82 was used in Flow Cytometry to show that under the influence of IL-2 and TGF-β, suppressive Foxp3+RORγt+ T cells that express and secrete IL-17 can be produced in vitro and RA further contributes to stabilize this phenotype.

Thu Oct 21 00:00:00 EDT 2021

Antigen dominance hierarchies shape TCF1⁺ progenitor CD8 T cell phenotypes in tumors.

Cell

Burger ML,Cruz AM,Crossland GE,Gaglia G,Ritch CC,Blatt SE,Bhutkar A,Canner D,Kienka T,Tavana SZ,Barandiaran AL,Garmilla A,Schenkel JM,Hillman M,de Los Rios Kobara I,Li A,Jaeger AM,Hwang WL,Westcott PMK,Manos MP,Holovatska MM,Hodi FS,Regev A,Santagata S,Jacks T

17-6981-82 was used in Flow cytometry/Cell sorting to conclude that vaccination eliminated CCR6+ TCF1+ cells and dramatically improved the subdominant response, highlighting a strategy to optimally engage concurrent neoantigen responses against tumors.

Thu Sep 16 00:00:00 EDT 2021

Gut-Innervating Nociceptor Neurons Regulate Peyer's Patch Microfold Cells and SFB Levels to Mediate Salmonella Host Defense.

Cell

Lai NY,Musser MA,Pinho-Ribeiro FA,Baral P,Jacobson A,Ma P,Potts DE,Chen Z,Paik D,Soualhi S,Yan Y,Misra A,Goldstein K,Lagomarsino VN,Nordstrom A,Sivanathan KN,Wallrapp A,Kuchroo VK,Nowarski R,Starnbach MN,Shi H,Surana NK,An D,Wu C,Huh JR,Rao M,Chiu IM

17-6981-82 was used in Flow Cytometry to reveal a major role for nociceptor neurons in sensing and defending against enteric pathogens.

Thu Jan 09 00:00:00 EST 2020

Regulatory T Cells License Macrophage Pro-Resolving Functions During Atherosclerosis Regression.

Circulation research

Sharma M,Schlegel MP,Afonso MS,Brown EJ,Rahman K,Weinstock A,Sansbury BE,Corr EM,van Solingen C,Koelwyn GJ,Shanley LC,Beckett L,Peled D,Lafaille JJ,Spite M,Loke P,Fisher EA,Moore KJ

17-6981 was used in Flow cytometry/Cell sorting to establish essential roles for Tregs in resolving atherosclerotic cardiovascular disease and provide mechanistic insight into the pathways governing plaque remodeling and regression of disease.

Fri Jul 17 00:00:00 EDT 2020

Acetate coordinates neutrophil and ILC3 responses against C. difficile through FFAR2.

The Journal of experimental medicine

Fachi JL,Sécca C,Rodrigues PB,Mato FCP,Di Luccia B,Felipe JS,Pral LP,Rungue M,Rocha VM,Sato FT,Sampaio U,Clerici MTPS,Rodrigues HG,Câmara NOS,Consonni SR,Vieira AT,Oliveira SC,Mackay CR,Layden BT,Bortoluci KR,Colonna M,Vinolo MAR

17-6981 was used in Flow cytometry/Cell sorting to study the impact of a microbiota-derived metabolite, short-chain fatty acid acetate, on an acute mouse model of CDI.

Mon Mar 02 00:00:00 EST 2020

Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells.

Nature communications

Harsha Krovi S,Zhang J,Michaels-Foster MJ,Brunetti T,Loh L,Scott-Browne J,Gapin L

17-6981 was used in Flow cytometry/Cell sorting to highlight the common requirements for the development of innate-like T cells with a focus on how Hivep3 impacts the maturation of these lymphocytes.

Mon Dec 07 00:00:00 EST 2020

Intestinal microbiota-derived short-chain fatty acids regulation of immune cell IL-22 production and gut immunity.

Nature communications

Yang W,Yu T,Huang X,Bilotta AJ,Xu L,Lu Y,Sun J,Pan F,Zhou J,Zhang W,Yao S,Maynard CL,Singh N,Dann SM,Liu Z,Cong Y

17-6981-82 was used in Flow Cytometry to demonstrate that microbiota-derived short-chain fatty acids (SCFAs) promote IL-22 production by CD4+ T cells and ILCs through G-protein receptor 41 (GPR41) and inhibiting histone deacetylase (HDAC).

Tue Sep 08 00:00:00 EDT 2020

Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells.

Cell reports

Shin B,Benavides GA,Geng J,Koralov SB,Hu H,Darley-Usmar VM,Harrington LE

17-6981 was used in Flow cytometry/Cell sorting to study the role of mitochondrial oxidative phosphorylation in pathogenic Th17 differentiation.

Tue Feb 11 00:00:00 EST 2020

Commensal Microbiota Promote Lung Cancer Development via γδ T Cells.

Cell

Jin C,Lagoudas GK,Zhao C,Bullman S,Bhutkar A,Hu B,Ameh S,Sandel D,Liang XS,Mazzilli S,Whary MT,Meyerson M,Germain R,Blainey PC,Fox JG,Jacks T

17-6981 was used in Flow cytometry/Cell sorting to link local microbiota-immune crosstalk to lung tumor development and thereby define key cellular and molecular mediators that may serve as effective targets in lung cancer intervention.

Thu Feb 21 00:00:00 EST 2019

Characterization of Transcriptional Regulatory Networks that Promote and Restrict Identities and Functions of Intestinal Innate Lymphoid Cells.

Immunity

Pokrovskii M,Hall JA,Ochayon DE,Yi R,Chaimowitz NS,Seelamneni H,Carriero N,Watters A,Waggoner SN,Littman DR,Bonneau R,Miraldi ER

17-6981 was used in Flow cytometry/Cell sorting to study regulatory interactions between transcription factors and genes within intestinal type 1, 2, and 3 innate lymphoid cells subsets.

Tue Jul 16 00:00:00 EDT 2019

Metabolite-Sensing Receptor Ffar2 Regulates Colonic Group 3 Innate Lymphoid Cells and Gut Immunity.

Immunity

Chun E,Lavoie S,Fonseca-Pereira D,Bae S,Michaud M,Hoveyda HR,Fraser GL,Gallini Comeau CA,Glickman JN,Fuller MH,Layden BT,Garrett WS

17-6981 was used in Flow cytometry/Cell sorting to demonstrate group 3 innate lymphoid cells are regulated by the metabolite-sensing receptor Ffar2.

Tue Nov 19 00:00:00 EST 2019

Transnuclear mice reveal Peyer's patch iNKT cells that regulate B-cell class switching to IgG1.

The EMBO journal

Clancy-Thompson E,Chen GZ,LaMarche NM,Ali LR,Jeong HJ,Crowley SJ,Boelaars K,Brenner MB,Lynch L,Dougan SK

17-6981-82 was used in Flow cytometry/Cell sorting to show that iNKT transnuclear mice, generated by somatic cell nuclear transfer, have increased tissue resident iNKT cells.

Mon Jul 15 00:00:00 EDT 2019

CD160 serves as a negative regulator of NKT cells in acute hepatic injury.

Nature communications

Kim TJ,Park G,Kim J,Lim SA,Kim J,Im K,Shin MH,Fu YX,Del Rio ML,Rodriguez-Barbosa JI,Yee C,Suh KS,Kim SJ,Ha SJ,Lee KM

17-6981-82 was used in Flow cytometry/Cell sorting to show that CD160 is not essential for NKT cell development.

Mon Jul 22 00:00:00 EDT 2019

A chemical biology toolbox to study protein methyltransferases and epigenetic signaling.

Nature communications

Scheer S,Ackloo S,Medina TS,Schapira M,Li F,Ward JA,Lewis AM,Northrop JP,Richardson PL,Kaniskan HÜ,Shen Y,Liu J,Smil D,McLeod D,Zepeda-Velazquez CA,Luo M,Jin J,Barsyte-Lovejoy D,Huber KVM,De Carvalho DD,Vedadi M,Zaph C,Brown PJ,Arrowsmith CH

17-6981 was used in Flow cytometry/Cell sorting to present a collection of chemical probes and associated reagents and data to elucidate the function of human and murine protein methyltransferases in cellular studies.

Thu Jan 03 00:00:00 EST 2019

IL-33 drives group 2 innate lymphoid cell-mediated protection during Clostridium difficile infection.

Nature communications

Frisbee AL,Saleh MM,Young MK,Leslie JL,Simpson ME,Abhyankar MM,Cowardin CA,Ma JZ,Pramoonjago P,Turner SD,Liou AP,Buonomo EL,Petri WA

17-6981-82 was used in Flow Cytometry to identify potential therapeutic immune pathways that aid in recovery during infection with the hypervirulent strain of Clostridium difficile, R20291.

Thu Jun 20 00:00:00 EDT 2019

Netrin-1 Alters Adipose Tissue Macrophage Fate and Function in Obesity.

Immunometabolism

Sharma M,Schlegel M,Brown EJ,Sansbury BE,Weinstock A,Afonso MS,Corr EM,van Solingen C,Shanley LC,Peled D,Ramasamy R,Schmidt AM,Spite M,Fisher EA,Moore KJ

17-6981-82 was used in Flow Cytometry to show that targeted deletion of netrin-1 in macrophages reprograms the ATM phenotype in obesity, leading to reduced adipose inflammation, and improved lipid handling and metabolic function.

Wed Sep 30 00:00:00 EDT 2020

Characterization and Purification of Mouse Mucosal-Associated Invariant T (MAIT) Cells.

Current protocols in immunology

Chen Z,Wang H,D'Souza C,Koay HF,Meehan B,Zhao Z,Pediongco T,Shi M,Zhu T,Wang B,Kjer-Nielsen L,Eckle SBG,Rossjohn J,Fairlie DP,Godfrey DI,Strugnell RA,McCluskey J,Corbett AJ

17-6981-82 was used in Flow cytometry/Cell sorting to provide a step-by-step protocol for mucosal-associated invariant T (MAIT) cell sorting and adoptive transfer.

Sun Dec 01 00:00:00 EST 2019

Ets1 suppresses atopic dermatitis by suppressing pathogenic T cell responses.

JCI insight

Lee CG,Kwon HK,Kang H,Kim Y,Nam JH,Won YH,Park S,Kim T,Kang K,Rudra D,Jun CD,Park ZY,Im SH

17-6981 was used in Flow cytometry/Cell sorting to reveal a protective role of Ets1 in restricting pathogenic Th cell responses and suggest a potential therapeutic target for atopic dermatitis (AD) treatment.

Thu Mar 07 00:00:00 EST 2019

The Crohn's disease polymorphism, ATG16L1 T300A, alters the gut microbiota and enhances the local Th1/Th17 response.

eLife

Lavoie S,Conway KL,Lassen KG,Jijon HB,Pan H,Chun E,Michaud M,Lang JK,Gallini Comeau CA,Dreyfuss JM,Glickman JN,Vlamakis H,Ananthakrishnan A,Kostic A,Garrett WS,Xavier RJ

17-6981 was used in Flow cytometry/Cell sorting to unravel the effects of ATG16L1 T300A on the microbiota and immune system, employing a gnotobiotic model using human fecal transfers into ATG16L1 T300A knock in mice.

Tue Jan 22 00:00:00 EST 2019

Commensals Suppress Intestinal Epithelial Cell Retinoic Acid Synthesis to Regulate Interleukin-22 Activity and Prevent Microbial Dysbiosis.

Immunity

Grizotte-Lake M,Zhong G,Duncan K,Kirkwood J,Iyer N,Smolenski I,Isoherranen N,Vaishnava S

17-6981 was used in Flow cytometry/Cell sorting to define a regulatory circuit wherein bacterial regulation of IEC-intrinsic RA synthesis protects microbial communities in the gut from excessive immune activity, achieving a balance that prevents colonization by enteric pathogens.

Tue Dec 18 00:00:00 EST 2018

Maternal gut bacteria promote neurodevelopmental abnormalities in mouse offspring.

Nature

Kim S,Kim H,Yim YS,Ha S,Atarashi K,Tan TG,Longman RS,Honda K,Littman DR,Choi GB,Huh JR

17-6981 was used in Flow cytometry/Cell sorting to suggest that defined gut commensal bacteria with a propensity to induce TH17 cells may increase the risk of neurodevelopmental disorders in the offspring of pregnant mothers undergoing immune system activation owing to infections or autoinflammatory syndromes.

Thu Sep 28 00:00:00 EDT 2017

T Helper 17 Promotes Induction of Antigen-Specific Gut-Mucosal Cytotoxic T Lymphocytes following Adenovirus Vector Vaccination.

Frontiers in immunology

Hemmi M,Tachibana M,Fujimoto N,Shoji M,Sakurai F,Kobiyama K,Ishii KJ,Akira S,Mizuguchi H

17-6981 was used in Flow cytometry/Cell sorting to study how Th17 cells translate systemic type I interferon signalling into a gut-mucosal cytotoxic T lymphocyte response following adenoviral vector vaccination.

Wed Nov 20 00:00:00 EST 2019

Intestinal Batf3-dependent dendritic cells are required for optimal antiviral T-cell responses in adult and neonatal mice.

Mucosal immunology

Sun T,Rojas OL,Li C,Ward LA,Philpott DJ,Gommerman JL

17-6981 was used in Flow cytometry/Cell sorting to study the dendritic cell subset(s) that provoke antiviral responses within the gut.

Mon May 01 00:00:00 EDT 2017

MyD88 signaling in dendritic cells and the intestinal epithelium controls immunity against intestinal infection with C. rodentium.

PLoS pathogens

Friedrich C,Mamareli P,Thiemann S,Kruse F,Wang Z,Holzmann B,Strowig T,Sparwasser T,Lochner M

17-6981 was used in Flow cytometry/Cell sorting to suggest that MyD88 signaling in DC and IEC is both essential and sufficient to induce a full spectrum of host responses upon intestinal infection with C. rodentium.

Mon May 01 00:00:00 EDT 2017

Retinoic Acid Regulates Immune Responses by Promoting IL-22 and Modulating S100 Proteins in Viral Hepatitis.

Journal of immunology (Baltimore, Md. : 1950)

Jie Z,Liang Y,Yi P,Tang H,Soong L,Cong Y,Zhang K,Sun J

17-6981 was used in Flow cytometry/Cell sorting to determine the role of retinoic acid in IL-22 production during hepatitis infection.

Mon May 01 00:00:00 EDT 2017

Monoclonal Invariant NKT (iNKT) Cell Mice Reveal a Role for Both Tissue of Origin and the TCR in Development of iNKT Functional Subsets.

Journal of immunology (Baltimore, Md. : 1950)

Clancy-Thompson E,Chen GZ,Tyler PM,Servos MM,Barisa M,Brennan PJ,Ploegh HL,Dougan SK

17-6981 was used in Flow cytometry/Cell sorting to examine how T cell receptor specificity determines invariant natural killer T cell function.

Sat Jul 01 00:00:00 EDT 2017

RORγt-expressing cells attenuate cardiac remodeling after myocardial infarction.

PloS one

Enomoto D,Matsumoto K,Yamashita T,Kobayashi A,Maeda M,Nakayama H,Obana M,Fujio Y

17-6981 was used in Flow cytometry/Cell sorting to demonstrate that heterozygous deletion of Retinoic acid receptor-related orphan nuclear receptor γt (RORγt) results in aggravated cardiac remodeling and reduced capillary density following myocardial infarction, suggesting a role in tissue repair.

Fri Oct 13 00:00:00 EDT 2017

Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.

JCI insight

Tischner D,Grimm M,Kaur H,Staudenraus D,Carvalho J,Looso M,Günther S,Wanke F,Moos S,Siller N,Breuer J,Schwab N,Zipp F,Waisman A,Kurschus FC,Offermanns S,Wettschureck N

17-6981 was used in Flow cytometry/Cell sorting to identify functionally distinct adaptive GPCR heterogeneity patternation in mice.

Thu Aug 03 00:00:00 EDT 2017

CD4(+) T-cell survival in the GI tract requires dectin-1 during fungal infection.

Mucosal immunology

Drummond RA,Dambuza IM,Vautier S,Taylor JA,Reid DM,Bain CC,Underhill DM,Masopust D,Kaplan DH,Brown GD

17-6981 was used in Flow cytometry/Cell sorting to study the role of Dectin-1 in the coordination of fungal-specific CD4(+) T-cell responses in the GI tract.

Tue Mar 01 00:00:00 EST 2016

Interleukin (IL)-21 promotes intestinal IgA response to microbiota.

Mucosal immunology

Cao AT,Yao S,Gong B,Nurieva RI,Elson CO,Cong Y

17-6981 was used in Flow cytometry/Cell sorting to investigate the role of Th17 and Tfh cytokines in regulating the mucosal IgA response to enteric microbiota.

Tue Sep 01 00:00:00 EDT 2015

Intrahepatic innate lymphoid cells secrete IL-17A and IL-17F that are crucial for T cell priming in viral infection.

Journal of immunology (Baltimore, Md. : 1950)

Jie Z,Liang Y,Hou L,Dong C,Iwakura Y,Soong L,Cong Y,Sun J

17-6981 was used in Flow cytometry/Cell sorting to investigate the source and regulation of the IL-17 surge in the liver microenvironment that occurs early in the response to viral infection.

Tue Apr 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: MGC129539; Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-related orphan receptor C; Retinoid-related orphan receptor-gamma; ROR Gamma; ROR gamma t; RORgt; RP11-98D18.11-001; Thymus orphan receptor; TOR

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Gene Aliases: Nr1f3; Rorc; Rorg; RORgamma; Thor; TOR

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UniProt ID: (Mouse) P51450

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Entrez Gene ID: (Mouse) 19885

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Function(s)

transcription coactivator binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding oxysterol binding zinc ion binding sequence-specific DNA binding metal ion binding transcription factor activity, direct ligand regulated sequence-specific DNA binding C4 zinc finger nuclear receptor

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation xenobiotic metabolic process multicellular organism development regulation of glucose metabolic process regulation of steroid metabolic process cell differentiation interleukin-17 production circadian regulation of gene expression T cell differentiation in thymus cellular response to sterol T-helper cell differentiation positive regulation of circadian rhythm steroid hormone mediated signaling pathway regulation of gamma-delta T cell differentiation regulation of fat cell differentiation positive regulation of transcription, DNA-templated alpha-beta T cell differentiation rhythmic process lymph node development mucosal-associated lymphoid tissue development Peyer's patch development adipose tissue development regulation of transcription involved in cell fate commitment negative regulation of thymocyte apoptotic process T-helper 17 cell differentiation

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ROR gamma (t) Monoclonal Antibody (B2D), APC, eBioscience™

ROR gamma (t) Antibody (17-6981-82) in Flow

Product Details

17-6981-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

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Type

Clone

Conjugate

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Form

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