Invitrogen

CD34 Monoclonal Antibody (4H11), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

53 Published Figures

52 References

View all (163) CD34 antibodies

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CD34 Antibody (25-0349-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45 FITC (Product # 11-0459-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD34 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD34 Antibody — Figure 4 Effects of the NL and RUX combination on primitive and quiescent (CFSE max ) CML and normal CD34 + cells. (A) CML and (B) normal CD34 + cells (n = 3) were stained with CFSE and either left UT, or treated with NL (5 uM) or RUX (200 nM) or their combination, and cultured. At 72 hours, the percentage of starting CD34 + cells recovered within each division in each treatment arm was calculated by recording the number of viable cells seeded initially in each culture and their number following different treatment conditions. Levels of CFSE fluorescence was used to measure the percentage of cells within each division as explained previously. 32 (C) Percentage of total number of starting CML, and (D) normal CD34 + cells recovered in each arm following treatment was also recorded at 72 hours. Percentage of apoptotic cells within the undivided (CFSE max ) population was measured by gating on the population double-positive for maximal CFSE expression, and Annexin-V staining at 72 hours for both (E) CML and (F) normal CD34 + cells. (G) Sorted CML, BCR-ABL + (by FISH) CD34 + CD38 - cells (n = 3) were cultured as in (A) for 72 hours. Percentage of viable cells was measured by gating on the double-negative population following Annexin-V/DAPI staining. The results were then normalized against UT within each sample. All data from independent experiments are presented as mean +- SEM. Significance values: * P < .05; + P < .01; ++ P < .001. {TM}

Product Details

25-0349-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 52 publications 52 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

4H11

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1963576

Product Specific Information

Description: The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of this family are single pass transmembrane proteins with a heavily glycosylated extracellular and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage by neuraminidase, chymopapain, or O-glycoprotease. According to this analysis, the 4H11 antibody belongs to class III, indicating that it reacts with a protein epitope.

Applications Reported: This 4H11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 4H11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Exercise combined with administration of adipose-derived stem cells ameliorates neuropathic pain after spinal cord injury.

Neural regeneration research

Cheng X,Mao GP,Hu WJ,Yu ZR,Xu YY,Chen W,Li X,Zeng XL,Zhang WW,Chen JW,Wan Y,Wang L

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2023

Celecoxib induces adipogenic differentiation of hemangioma-derived mesenchymal stem cells through the PPAR-γ pathway in vitro and in vivo.

Experimental and therapeutic medicine

Wang Y,Kong L,Sun B,Cui J,Shen W

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2022

Mitochondrial oxidative phosphorylation is dispensable for survival of CD34⁺ chronic myeloid leukemia stem and progenitor cells.

Cell death & disease

Yan JS,Yang MY,Zhang XH,Luo CH,Du CK,Jiang Y,Dong XJ,Wang ZM,Yang LX,Li YD,Xia L,Lu Y

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Wed Apr 20 00:00:00 EDT 2022

Human placental mesenchymal stem cells regulate inflammation via the NF‑κB signaling pathway.

Experimental and therapeutic medicine

Liu Y,Zhang X,Hu Y,Kang M,Wu Y,Wang Y,Deng C

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Angiogenic stem cell delivery platform to augment post-infarction neovasculature and reverse ventricular remodeling.

Scientific reports

Shin HS,Thakore A,Tada Y,Pedroza AJ,Ikeda G,Chen IY,Chan D,Jaatinen KJ,Yajima S,Pfrender EM,Kawamura M,Yang PC,Wu JC,Appel EA,Fischbein MP,Woo Y,Shudo Y

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Thu Oct 20 00:00:00 EDT 2022

Decoding the pathogenesis of Diamond-Blackfan anemia using single-cell RNA-seq.

Cell discovery

Wang B,Wang C,Wan Y,Gao J,Ma Y,Zhang Y,Tong J,Zhang Y,Liu J,Chang L,Xu C,Shen B,Chen Y,Jiang E,Kurita R,Nakamura Y,Lim KC,Engel JD,Zhou J,Cheng T,Zhu X,Zhu P,Shi L

25-0349-42 was used in Flow cytometry/Cell sorting to demonstrate that interferon-mediated cell cycle control underlies the clinical efficacy of glucocorticoids.

Tue May 10 00:00:00 EDT 2022

LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling.

International journal of medical sciences

Mu R,Chen B,Bi B,Yu H,Liu J,Li J,He M,Rong L,Liu B,Liu K,Zhu L,Shi X,Shuai Y,Jin L

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Mon Aug 08 00:00:00 EDT 2022

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.

Bioengineered

Jiang W,Zhang J,Zhang X,Fan C,Huang J

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Endothelial GABBR2 Regulates Post-ischemic Angiogenesis by Inhibiting the Glycolysis Pathway.

Frontiers in cardiovascular medicine

Zhang H,Zhou H,Yuan J,Nan Y,Liu J

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

Development, characterization, and hematopoietic differentiation of Griscelli syndrome type 2 induced pluripotent stem cells.

Stem cell research & therapy

Güney-Esken G,Erol ÖD,Pervin B,Gürhan Sevinç G,Önder T,Bilgiç E,Korkusuz P,Günel-Özcan A,Uçkan-Çetinkaya D,Aerts-Kaya F

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Targeting the epichaperome as an effective precision medicine approach in a novel PML-SYK fusion acute myeloid leukemia.

NPJ precision oncology

Sugita M,Wilkes DC,Bareja R,Eng KW,Nataraj S,Jimenez-Flores RA,Yan L,De Leon JP,Croyle JA,Kaner J,Merugu S,Sharma S,MacDonald TY,Noorzad Z,Panchal P,Pancirer D,Cheng S,Xiang JZ,Olson L,Van Besien K,Rickman DS,Mathew S,Tam W,Rubin MA,Beltran H,Sboner A,Hassane DC,Chiosis G,Elemento O,Roboz GJ,Mosquera JM,Guzman ML

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Wed May 26 00:00:00 EDT 2021

Phenotypic and functional characterization of synovial fluid-derived fibroblast-like synoviocytes in rheumatoid arthritis.

Scientific reports

Køster D,Egedal JH,Lomholt S,Hvid M,Jakobsen MR,Müller-Ladner U,Eibel H,Deleuran B,Kragstrup TW,Neumann E,Nielsen MA

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Fri Nov 12 00:00:00 EST 2021

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

Resveratrol rescues TNF‑α‑induced inhibition of osteogenesis in human periodontal ligament stem cells via the ERK1/2 pathway.

Molecular medicine reports

Yuan J,Wang X,Ma D,Gao H,Zheng D,Zhang J

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

25-0349 was used in Flow cytometry/Cell sorting to investigate if downregulated membrane expression of CD18 characterises a primitive population of human hematopoietic repopulating cells.

Tue Apr 28 00:00:00 EDT 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

25-0349 was used in Flow cytometry/Cell sorting to investigate if downregulated membrane expression of CD18 characterises a primitive population of human hematopoietic repopulating cells.

Tue Apr 28 00:00:00 EDT 2020

Genome-Wide Transcriptional Regulation of the Long Non-coding RNA Steroid Receptor RNA Activator in Human Erythroblasts.

Frontiers in genetics

Sawaengdee W,Cui K,Zhao K,Hongeng S,Fucharoen S,Wongtrakoongate P

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Chemerin enhances the adhesion and migration of human endothelial progenitor cells and increases lipid accumulation in mice with atherosclerosis.

Lipids in health and disease

Jia J,Yu F,Xiong Y,Wei W,Ma H,Nisi F,Song X,Yang L,Wang D,Yuan G,Zhou H

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Sun Sep 20 00:00:00 EDT 2020

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

Identification and characterization of the cellular subclones that contribute to the pathogenesis of mantle cell lymphoma.

Genes & diseases

Tang J,Zhang L,Zhou T,Sun Z,Kong L,Jing L,Xing H,Wu H,Liu Y,Zhou S,Li J,Chen M,Xu F,Tang J,Ma T,Hu M,Liu D,Guo J,Zhu X,Chen Y,Ye T,Wang J,Li X,Xing HR

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

Therapeutic Effects of Human Urine-Derived Stem Cells in a Rat Model of Cisplatin-Induced Acute Kidney Injury In Vivo and In Vitro.

Stem cells international

Sun B,Luo X,Yang C,Liu P,Yang Y,Dong X,Yang Z,Xu J,Zhang Y,Li L

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.

Molecular medicine reports

Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Regnase-1-mediated post-transcriptional regulation is essential for hematopoietic stem and progenitor cell homeostasis.

Nature communications

Kidoya H,Muramatsu F,Shimamura T,Jia W,Satoh T,Hayashi Y,Naito H,Kunisaki Y,Arai F,Seki M,Suzuki Y,Osawa T,Akira S,Takakura N

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Wed Mar 06 00:00:00 EST 2019

Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer.

Cancers

Huang JY,Wang YY,Lo S,Tseng LM,Chen DR,Wu YC,Hou MF,Yuan SF

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Fri Dec 20 00:00:00 EST 2019

Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5.

BMC biotechnology

Lv Y,Xiao FJ,Wang Y,Zou XH,Wang H,Wang HY,Wang LS,Lu ZZ

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Wed Apr 24 00:00:00 EDT 2019

Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies.

Cell reports

Mair B,Tomic J,Masud SN,Tonge P,Weiss A,Usaj M,Tong AHY,Kwan JJ,Brown KR,Titus E,Atkins M,Chan KSK,Munsie L,Habsid A,Han H,Kennedy M,Cohen B,Keller G,Moffat J

25-0349-42 was used in Flow Cytometry to establish parameters for genome-wide screens in hPSCs to facilitate the characterization of unappreciated genetic regulators of hPSC biology.

Tue Apr 09 00:00:00 EDT 2019

A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1.

Developmental cell

Böiers C,Richardson SE,Laycock E,Zriwil A,Turati VA,Brown J,Wray JP,Wang D,James C,Herrero J,Sitnicka E,Karlsson S,Smith AJH,Jacobsen SEW,Enver T

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Mon Feb 05 00:00:00 EST 2018

Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.

Experimental and therapeutic medicine

Yan N,Xu J,Zhao C,Wu Y,Gao F,Li C,Zhou W,Xiao T,Zhou X,Shao Q,Xia S

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Improving hematopoietic recovery through modeling and modulation of the mesenchymal stromal cell secretome.

Stem cell research & therapy

Liu FD,Tam K,Pishesha N,Poon Z,Van Vliet KJ

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Wed Oct 24 00:00:00 EDT 2018

EDAG promotes the expansion and survival of human CD34+ cells.

PloS one

Zhao K,Zheng WW,Dong XM,Yin RH,Gao R,Li X,Liu JF,Zhan YQ,Yu M,Chen H,Ge CH,Ning HM,Yang XM,Li CY

25-0349 was used in Flow cytometry/Cell sorting to provide novel insight into EDAG in regulation of expansion and survival of human hematopoietic stem/progenitor cells.

Tue Feb 06 00:00:00 EST 2018

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

A high-yield isolation and enrichment strategy for human lung microvascular endothelial cells.

Pulmonary circulation

Gaskill C,Majka SM

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats.

Stem cell research & therapy

Yang H,Wu S,Feng R,Huang J,Liu L,Liu F,Chen Y

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Tue Nov 21 00:00:00 EST 2017

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.

Stem cell research & therapy

Li J,Mao Q,He J,She H,Zhang Z,Yin C

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

Human Induced Pluripotent Stem Cell-Derived Macrophages Share Ontogeny with MYB-Independent Tissue-Resident Macrophages.

Stem cell reports

Buchrieser J,James W,Moore MD

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2017

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.

Stem cells international

Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2016

Microvesicles from Mesenchymal Stromal Cells Are Involved in HPC-Microenvironment Crosstalk in Myelodysplastic Patients.

PloS one

Muntión S,Ramos TL,Diez-Campelo M,Rosón B,Sánchez-Abarca LI,Misiewicz-Krzeminska I,Preciado S,Sarasquete ME,de Las Rivas J,González M,Sánchez-Guijo F,Del Cañizo MC

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Tue Jul 12 00:00:00 EDT 2016

A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis.

Bone marrow research

Juneja SC,Viswanathan S,Ganguly M,Veillette C

25-0349 was used in Flow cytometry/Cell sorting to show that the MSCs derived from such bone marrow specimens exhibited MSCs characteristics and generated micromass cartilages, the recipe for regenerative medicine for osteoarthritis.

Fri Apr 08 00:00:00 EDT 2016

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Single-cell profiling of human megakaryocyte-erythroid progenitors identifies distinct megakaryocyte and erythroid differentiation pathways.

Genome biology

Psaila B,Barkas N,Iskander D,Roy A,Anderson S,Ashley N,Caputo VS,Lichtenberg J,Loaiza S,Bodine DM,Karadimitris A,Mead AJ,Roberts I

25-0349 was used in Flow cytometry/Cell sorting to clarify the cellular hierarchy in human megakaryocyte/erythroid lineage commitment and highlight the importance of using a combination of single-cell approaches to dissect cellular heterogeneity.

Tue May 03 00:00:00 EDT 2016

Functional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells.

American journal of physiology. Cell physiology

Hänggi P,Telezhkin V,Kemp PJ,Schmugge M,Gassmann M,Goede JS,Speer O,Bogdanova A

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

Differences in the Osteogenic Differentiation Capacity of Omental Adipose-Derived Stem Cells in Obese Patients With and Without Metabolic Syndrome.

Endocrinology

Oliva-Olivera W,Leiva Gea A,Lhamyani S,Coín-Aragüez L,Alcaide Torres J,Bernal-López MR,García-Luna PP,Morales Conde S,Fernández-Veledo S,El Bekay R,Tinahones FJ

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment.

Nature communications

Mian SA,Rouault-Pierre K,Smith AE,Seidl T,Pizzitola I,Kizilors A,Kulasekararaj AG,Bonnet D,Mufti GJ

25-0349 was used in Flow cytometry/Cell sorting to demonstrate that mutations to SF3B1 can arise from CD34(+)CD38(-)CD45RA(-)CD90(+)CD49f(+) haematopoietic stem cells and is an initiating event in myelodysplastic syndrome patients.

Tue Dec 08 00:00:00 EST 2015

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

25-0349 was used in Flow cytometry/Cell sorting to investigate the role of inductive cues from the vascular niche in coordinating and sustaining haematopoietic specification.

Thu Jul 17 00:00:00 EDT 2014

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

25-0349 was used in Flow cytometry/Cell sorting to investigate the role of inductive cues from the vascular niche in coordinating and sustaining haematopoietic specification.

Thu Jul 17 00:00:00 EDT 2014

JAK2/STAT5 inhibition by nilotinib with ruxolitinib contributes to the elimination of CML CD34+ cells in vitro and in vivo.

Blood

Gallipoli P,Cook A,Rhodes S,Hopcroft L,Wheadon H,Whetton AD,Jørgensen HG,Bhatia R,Holyoake TL

Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry

Thu Aug 28 00:00:00 EDT 2014

Developmental transcriptome analysis of human erythropoiesis.

Human molecular genetics

Shi L,Lin YH,Sierant MC,Zhu F,Cui S,Guan Y,Sartor MA,Tanabe O,Lim KC,Engel JD

25-0349 was used in Flow cytometry/Cell sorting to perform RNA-seq on CD34(+) cells after ex vivo differentiation from the earliest into the most mature erythroid cell stages.

Mon Sep 01 00:00:00 EDT 2014

Endothelial damage and vascular calcification in patients with chronic kidney disease.

American journal of physiology. Renal physiology

Soriano S,Carmona A,Triviño F,Rodriguez M,Alvarez-Benito M,Martín-Malo A,Alvarez-Lara MA,Ramírez R,Aljama P,Carracedo J

25-0349 was used in Flow cytometry/Cell sorting to investigate how EPCs, through OCN expression, may directly participate in the process of VC.

Mon Dec 01 00:00:00 EST 2014

Lysine-specific demethylase 1 is a therapeutic target for fetal hemoglobin induction.

Nature medicine

Shi L,Cui S,Engel JD,Tanabe O

25-0349 was used in Flow cytometry/Cell sorting to examine the effect of lysine-specific demethylase 1 inhibition on the expression of foetal γ-globin.

Fri Mar 01 00:00:00 EST 2013

CXC chemokine receptor 4 is essential for maintenance of renal cell carcinoma-initiating cells and predicts metastasis.

Stem cells (Dayton, Ohio)

Gassenmaier M,Chen D,Buchner A,Henkel L,Schiemann M,Mack B,Schendel DJ,Zimmermann W,Pohla H

25-0349 was used in Flow cytometry/Cell sorting to study the expression of CXC chemokine receptor 4 in a subpopulation of tumour-initiating cells in renal cell carcinoma cell lines and study its role in their maintenance.

Thu Aug 01 00:00:00 EDT 2013

Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.

PloS one

Rosu-Myles M,She YM,Fair J,Muradia G,Mehic J,Menendez P,Prasad SS,Cyr TD

25-0349 was used in Flow cytometry/Cell sorting to provide evidence of heterogeneity among bone marrow stromal cells and identify potential candidate proteins for quantifying MSCs in vitro.

Thu Dec 13 00:00:00 EST 2012

Bioinformatics

Protein Aliases: CD34; CD34 antigen; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2

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Gene Aliases: CD34

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UniProt ID: (Human) P28906

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Entrez Gene ID: (Human) 947

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Function(s)

transcription factor binding carbohydrate binding sulfate binding cell adhesion molecule

Process(es)

tissue homeostasis endothelial cell proliferation glomerular filtration endothelium development cell-matrix adhesion signal transduction regulation of blood pressure cell proliferation positive regulation of gene expression negative regulation of gene expression single organismal cell-cell adhesion hemopoiesis negative regulation of blood coagulation negative regulation of tumor necrosis factor production positive regulation of interleukin-10 production mesangial cell-matrix adhesion paracrine signaling positive regulation of odontogenesis negative regulation of nitric oxide biosynthetic process positive regulation of angiogenesis cell motility regulation of immune response leukocyte migration transdifferentiation vascular wound healing hematopoietic stem cell proliferation positive regulation of transforming growth factor beta production positive regulation of granulocyte colony-stimulating factor production extracellular exosome assembly glomerular endothelium development stem cell proliferation metanephric glomerular mesangial cell differentiation negative regulation of cellular response to heat negative regulation of cellular response to hypoxia negative regulation of interleukin-2 secretion positive regulation of glial cell-derived neurotrophic factor secretion negative regulation of neuron death positive regulation of vasculogenesis

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CD34 Monoclonal Antibody (4H11), PE-Cyanine7, eBioscience™

CD34 Antibody (25-0349-42) in Flow

Product Details

25-0349-42

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