Invitrogen

CD34 Monoclonal Antibody (4H11), Super Bright™ 600, eBioscience™

FIGURE: 1 / 25

CD34 Antibody (63-0349-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45 FITC (Product # 11-0459-42) and Mouse IgG1 K Isotype Control Super Bright 600 (Product # 63-4714-82) (left) or Anti-Human CD34 Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

63-0349-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 27 publications 27 publications

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 600, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

4H11

Conjugate

Super Bright™ 600 Super Bright™ 600 Super Bright™ 600

Excitation/Emission Max

414/601 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2663625

Product Specific Information

Description: The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of this family are single pass transmembrane proteins with a heavily glycosylated extracellular and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage by neuraminidase, chymopapain, or O-glycoprotease. According to this analysis, the 4H11 antibody belongs to class III, indicating that it reacts with a protein epitope.

Applications Reported: This 4H11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 4H11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test.

Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Exercise combined with administration of adipose-derived stem cells ameliorates neuropathic pain after spinal cord injury.

Neural regeneration research

Cheng X,Mao GP,Hu WJ,Yu ZR,Xu YY,Chen W,Li X,Zeng XL,Zhang WW,Chen JW,Wan Y,Wang L

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2023

Celecoxib induces adipogenic differentiation of hemangioma-derived mesenchymal stem cells through the PPAR-γ pathway in vitro and in vivo.

Experimental and therapeutic medicine

Wang Y,Kong L,Sun B,Cui J,Shen W

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2022

Mitochondrial oxidative phosphorylation is dispensable for survival of CD34⁺ chronic myeloid leukemia stem and progenitor cells.

Cell death & disease

Yan JS,Yang MY,Zhang XH,Luo CH,Du CK,Jiang Y,Dong XJ,Wang ZM,Yang LX,Li YD,Xia L,Lu Y

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Wed Apr 20 00:00:00 EDT 2022

Human placental mesenchymal stem cells regulate inflammation via the NF‑κB signaling pathway.

Experimental and therapeutic medicine

Liu Y,Zhang X,Hu Y,Kang M,Wu Y,Wang Y,Deng C

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Angiogenic stem cell delivery platform to augment post-infarction neovasculature and reverse ventricular remodeling.

Scientific reports

Shin HS,Thakore A,Tada Y,Pedroza AJ,Ikeda G,Chen IY,Chan D,Jaatinen KJ,Yajima S,Pfrender EM,Kawamura M,Yang PC,Wu JC,Appel EA,Fischbein MP,Woo Y,Shudo Y

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Thu Oct 20 00:00:00 EDT 2022

Decoding the pathogenesis of Diamond-Blackfan anemia using single-cell RNA-seq.

Cell discovery

Wang B,Wang C,Wan Y,Gao J,Ma Y,Zhang Y,Tong J,Zhang Y,Liu J,Chang L,Xu C,Shen B,Chen Y,Jiang E,Kurita R,Nakamura Y,Lim KC,Engel JD,Zhou J,Cheng T,Zhu X,Zhu P,Shi L

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Tue May 10 00:00:00 EDT 2022

LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling.

International journal of medical sciences

Mu R,Chen B,Bi B,Yu H,Liu J,Li J,He M,Rong L,Liu B,Liu K,Zhu L,Shi X,Shuai Y,Jin L

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Mon Aug 08 00:00:00 EDT 2022

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.

Bioengineered

Jiang W,Zhang J,Zhang X,Fan C,Huang J

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Endothelial GABBR2 Regulates Post-ischemic Angiogenesis by Inhibiting the Glycolysis Pathway.

Frontiers in cardiovascular medicine

Zhang H,Zhou H,Yuan J,Nan Y,Liu J

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Modeling of endothelial cell dysfunction using human induced pluripotent stem cells derived from patients with end-stage renal disease.

Kidney research and clinical practice

Kim KW,Shin YJ,Kim BM,Cui S,Ko EJ,Lim SW,Yang CW,Chung BH

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Immunocytochemistry

Wed Dec 01 00:00:00 EST 2021

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

Development, characterization, and hematopoietic differentiation of Griscelli syndrome type 2 induced pluripotent stem cells.

Stem cell research & therapy

Güney-Esken G,Erol ÖD,Pervin B,Gürhan Sevinç G,Önder T,Bilgiç E,Korkusuz P,Günel-Özcan A,Uçkan-Çetinkaya D,Aerts-Kaya F

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Targeting the epichaperome as an effective precision medicine approach in a novel PML-SYK fusion acute myeloid leukemia.

NPJ precision oncology

Sugita M,Wilkes DC,Bareja R,Eng KW,Nataraj S,Jimenez-Flores RA,Yan L,De Leon JP,Croyle JA,Kaner J,Merugu S,Sharma S,MacDonald TY,Noorzad Z,Panchal P,Pancirer D,Cheng S,Xiang JZ,Olson L,Van Besien K,Rickman DS,Mathew S,Tam W,Rubin MA,Beltran H,Sboner A,Hassane DC,Chiosis G,Elemento O,Roboz GJ,Mosquera JM,Guzman ML

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Wed May 26 00:00:00 EDT 2021

Phenotypic and functional characterization of synovial fluid-derived fibroblast-like synoviocytes in rheumatoid arthritis.

Scientific reports

Køster D,Egedal JH,Lomholt S,Hvid M,Jakobsen MR,Müller-Ladner U,Eibel H,Deleuran B,Kragstrup TW,Neumann E,Nielsen MA

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Fri Nov 12 00:00:00 EST 2021

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

Resveratrol rescues TNF‑α‑induced inhibition of osteogenesis in human periodontal ligament stem cells via the ERK1/2 pathway.

Molecular medicine reports

Yuan J,Wang X,Ma D,Gao H,Zheng D,Zhang J

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

Genome-Wide Transcriptional Regulation of the Long Non-coding RNA Steroid Receptor RNA Activator in Human Erythroblasts.

Frontiers in genetics

Sawaengdee W,Cui K,Zhao K,Hongeng S,Fucharoen S,Wongtrakoongate P

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Chemerin enhances the adhesion and migration of human endothelial progenitor cells and increases lipid accumulation in mice with atherosclerosis.

Lipids in health and disease

Jia J,Yu F,Xiong Y,Wei W,Ma H,Nisi F,Song X,Yang L,Wang D,Yuan G,Zhou H

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Sun Sep 20 00:00:00 EDT 2020

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Tue Apr 28 00:00:00 EDT 2020

Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer.

Cancers

Huang JY,Wang YY,Lo S,Tseng LM,Chen DR,Wu YC,Hou MF,Yuan SF

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Fri Dec 20 00:00:00 EST 2019

Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5.

BMC biotechnology

Lv Y,Xiao FJ,Wang Y,Zou XH,Wang H,Wang HY,Wang LS,Lu ZZ

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Wed Apr 24 00:00:00 EDT 2019

Improving hematopoietic recovery through modeling and modulation of the mesenchymal stromal cell secretome.

Stem cell research & therapy

Liu FD,Tam K,Pishesha N,Poon Z,Van Vliet KJ

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Wed Oct 24 00:00:00 EDT 2018

A high-yield isolation and enrichment strategy for human lung microvascular endothelial cells.

Pulmonary circulation

Gaskill C,Majka SM

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Functional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells.

American journal of physiology. Cell physiology

Hänggi P,Telezhkin V,Kemp PJ,Schmugge M,Gassmann M,Goede JS,Speer O,Bogdanova A

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

Published figure using CD34 monoclonal antibody (Product # 63-0349-42) in Flow Cytometry

Thu Jul 17 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD34; CD34 antigen; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2

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Gene Aliases: CD34

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UniProt ID: (Human) P28906

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Entrez Gene ID: (Human) 947

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Function(s)

transcription factor binding carbohydrate binding sulfate binding cell adhesion molecule

Process(es)

tissue homeostasis endothelial cell proliferation glomerular filtration endothelium development cell-matrix adhesion signal transduction regulation of blood pressure cell proliferation positive regulation of gene expression negative regulation of gene expression single organismal cell-cell adhesion hemopoiesis negative regulation of blood coagulation negative regulation of tumor necrosis factor production positive regulation of interleukin-10 production mesangial cell-matrix adhesion paracrine signaling positive regulation of odontogenesis negative regulation of nitric oxide biosynthetic process positive regulation of angiogenesis cell motility regulation of immune response leukocyte migration transdifferentiation vascular wound healing hematopoietic stem cell proliferation positive regulation of transforming growth factor beta production positive regulation of granulocyte colony-stimulating factor production extracellular exosome assembly glomerular endothelium development stem cell proliferation metanephric glomerular mesangial cell differentiation negative regulation of cellular response to heat negative regulation of cellular response to hypoxia negative regulation of interleukin-2 secretion positive regulation of glial cell-derived neurotrophic factor secretion negative regulation of neuron death positive regulation of vasculogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD34 Monoclonal Antibody (4H11), Super Bright™ 600, eBioscience™

CD34 Antibody (63-0349-42) in Flow

Product Details

63-0349-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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