Invitrogen

CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), PE-Cyanine7, eBioscience™

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CD163 Antibody (25-1639-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD11b APC (Product # 17-0118-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD163 PE-Cyanine7 (right). Cells in the monocyte gate were used for analysis.

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Product Details

25-1639-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 20 publications 20 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioGHI/61 (GHI/61)

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573413

Product Specific Information

Description: The eBioGHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family. CD163 is expressed by tissue macrophages, circulating blood monocytes, and some reports suggest that it may also be expressed by haematopoietic progenitor cells. CD163 functions as a high affinity scavenger receptor for the complex of haemoglobin and haptoglobin which forms to clear haemoglobin from the blood. The high affinity for CD163 is specific for the haemoglobin-haptoglobin complex, whereas haemoglobin or haptoglobin display no to low affinity toward CD163. Upon ligand binding, CD163 initiates signal transduction which leads to the production of IL-6 and IL-10. Furthermore, it has been demonstrated that cytokines such as IL-6 and IL-10 can induce expression of CD163, whereas proinflammatory stimuli, such as LPS or TNF alpha, can cause shedding of a soluble form of CD163, whose function is unclear.

Applications Reported: This eBioGHI/61 (GHI/61) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioGHI/61 (GHI/61) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

Germline Neurofibromin 1 mutation enhances the anti-tumour immune response and decreases juvenile myelomonocytic leukaemia tumourigenicity.

British journal of haematology

Wang W,Li X,Qin X,Miao Y,Zhang Y,Li S,Yao R,Yang Y,Yu L,Zhu H,Song L,Mao S,Wang X,Chen J,Feng H,Li Y

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Heme oxygenase-1 determines the cell fate of ferroptotic death of alveolar macrophages in COPD.

Frontiers in immunology

Li Y,Yang Y,Guo T,Weng C,Yang Y,Wang Z,Zhang L,Li W

25-1639-42 was used in Flow cytometry/Cell sorting to identify two populations of alveolar macrophages (AMs) in the human lung that were dysregulated in COPD patients.

Wed May 24 00:00:00 EDT 2023

SARS-CoV-2 Delta (B.1.617.2) variant replicates and induces syncytia formation in human induced pluripotent stem cell-derived macrophages.

PeerJ

Thaweerattanasinp T,Wanitchang A,Saenboonrueng J,Srisutthisamphan K,Wanasen N,Sungsuwan S,Jongkaewwattana A,Chailangkarn T

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2023

A positive feedback loop: RAD18-YAP-TGF-β between triple-negative breast cancer and macrophages regulates cancer stemness and progression.

Cell death discovery

Yan X,He Y,Yang S,Zeng T,Hua Y,Bao S,Yang F,Duan N,Sun C,Liang Y,Fu Z,Huang X,Li W,Yin Y

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Tue Apr 12 00:00:00 EDT 2022

M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties.

Cell death discovery

Zhao G,Ding L,Yu H,Wang W,Wang H,Hu Y,Qin L,Deng G,Xie B,Li G,Qi L

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Thu Aug 04 00:00:00 EDT 2022

M1 macrophage-derived exosomes and their key molecule lncRNA HOTTIP suppress head and neck squamous cell carcinoma progression by upregulating the TLR5/NF-κB pathway.

Cell death & disease

Jiang H,Zhou L,Shen N,Ning X,Wu D,Jiang K,Huang X

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Thu Feb 24 00:00:00 EST 2022

TcpC Inhibits M1 but Promotes M2 Macrophage Polarization via Regulation of the MAPK/NF-κB and Akt/STAT6 Pathways in Urinary Tract Infection.

Cells

Fang J,Ou Q,Wu B,Li S,Wu M,Qiu J,Cen N,Hu K,Che Y,Ma Y,Pan J

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Sun Aug 28 00:00:00 EDT 2022

PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.

CNS neuroscience & therapeutics

Zhang H,Wang Y,Zhao Y,Liu T,Wang Z,Zhang N,Dai Z,Wu W,Cao H,Feng S,Zhang L,Cheng Q,Liu Z

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Pulmonary transplantation of alpha-1 antitrypsin (AAT)-transgenic macrophages provides a source of functional human AAT in vivo.

Gene therapy

Janosz E,Hetzel M,Spielmann H,Tumpara S,Rossdam C,Schwabbauer M,Kloos D,von Kaisenberg C,Schambach A,Buettner FFR,Janciauskiene S,Lachmann N,Moritz T

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Dysregulation of the CD163-Haptoglobin Axis in the Airways of COPD Patients.

Cells

Higham A,Baker JM,Jackson N,Shah R,Lea S,Singh D

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Tue Dec 21 00:00:00 EST 2021

Macrophages induce malignant traits in mammary epithelium via IKKε/TBK1 kinases and the serine biosynthesis pathway.

EMBO molecular medicine

Wilcz-Villega E,Carter E,Ironside A,Xu R,Mataloni I,Holdsworth J,Jones W,Moreno Béjar R,Uhlik L,Bentham RB,Godinho SA,Dalli J,Grose R,Szabadkai G,Jones L,Hodivala-Dilke K,Bianchi K

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Fri Feb 07 00:00:00 EST 2020

Extracorporeal Hemadsorption versus Glucocorticoids during Cardiopulmonary Bypass: A Prospective, Randomized, Controlled Trial.

Cardiovascular therapeutics

Taleska Stupica G,Sostaric M,Bozhinovska M,Rupert L,Bosnic Z,Jerin A,Ihan A,Klokocovnik T,Podbregar M

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Patient iPSC-Derived Macrophages to Study Inborn Errors of the IFN-γ Responsive Pathway.

Cells

Haake K,Neehus AL,Buchegger T,Kühnel MP,Blank P,Philipp F,Oleaga-Quintas C,Schulz A,Grimley M,Goethe R,Jonigk D,Kalinke U,Boisson-Dupuis S,Casanova JL,Bustamante J,Lachmann N

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Wed Feb 19 00:00:00 EST 2020

Recruitment of monocytes and epigenetic silencing of intratumoral CYP7B1 primarily contribute to the accumulation of 27-hydroxycholesterol in breast cancer.

American journal of cancer research

Shi SZ,Lee EJ,Lin YJ,Chen L,Zheng HY,He XQ,Peng JY,Noonepalle SK,Shull AY,Pei FC,Deng LB,Tian XL,Deng KY,Shi H,Xin HB

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

TIM‑4 blockade of KCs combined with exogenous TGF‑β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts.

International journal of molecular medicine

Wu H,Xu X,Li J,Gong J,Li M

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2018

Biochanin a Enhances the Defense Against Salmonella enterica Infection Through AMPK/ULK1/mTOR-Mediated Autophagy and Extracellular Traps and Reversing SPI-1-Dependent Macrophage (MΦ) M2 Polarization.

Frontiers in cellular and infection microbiology

Zhao X,Tang X,Guo N,An Y,Chen X,Shi C,Wang C,Li Y,Li S,Xu H,Liu M,Wang Y,Yu L

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Fri Aug 30 00:00:00 EDT 2019

Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system.

Journal of neurovirology

Garcia-Mesa Y,Jay TR,Checkley MA,Luttge B,Dobrowolski C,Valadkhan S,Landreth GE,Karn J,Alvarez-Carbonell D

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Alternatively activated macrophages derived from THP-1 cells promote the fibrogenic activities of human dermal fibroblasts.

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society

Zhu Z,Ding J,Ma Z,Iwashina T,Tredget EE

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.

Bioscience reports

Zhong Y,Yi C

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Interleukin-25 fails to activate STAT6 and induce alternatively activated macrophages.

Immunology

Stolfi C,Caruso R,Franzè E,Sarra M,De Nitto D,Rizzo A,Pallone F,Monteleone G

Published figure using CD163 monoclonal antibody (Product # 25-1639-42) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: CD163; Hemoglobin scavenger receptor; macrophage-associated antigen; Scavenger receptor cysteine-rich type 1 protein M130; Soluble sCD163

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Gene Aliases: CD163; M130; MM130; SCARI1

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UniProt ID: (Human) Q86VB7

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Entrez Gene ID: (Human) 9332

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Function(s)

scavenger receptor activity protein binding serine protease

Process(es)

receptor-mediated endocytosis acute-phase response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), PE-Cyanine7, eBioscience™

CD163 Antibody (25-1639-42) in Flow

Product Details

25-1639-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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