Invitrogen

Ki-67 Monoclonal Antibody (SolA15), PE-Cyanine7, eBioscience™

FIGURE: 1 / 1

Ki-67 Antibody (25-5698-82) in Flow

C57Bl/6 splenocytes were unstimulated (left) or stimuated for 2 days with Anti-Mouse CD3 Functional Grade Purified (Product # 16-0031-82) (right). Cell were stained with Anti-Mouse CD19 APC (Product # 17-0193-82) followed by fixation and permeabilizatin using the Foxp3 Staining Buffer Set (Product # 00-5523-00) and subsequently stained with 0.06 µg of Anti-Mouse/Rat Ki-67 PE-Cyanine7. Cells in the lymphocyte gate were used for analysis.

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Product Details

25-5698-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 34 publications 34 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SolA15

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11220070

Product Specific Information

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).

The SolA15 antibody also recognizes human, non-human primate and canine Ki-67.

Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This SolA15 antibody has been tested by intracellular staining and flow cytometric analysisusing the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Meningeal lymphatic drainage promotes T cell responses against Toxoplasma gondii but is dispensable for parasite control in the brain.

eLife

Kovacs MA,Cowan MN,Babcock IW,Sibley LA,Still K,Batista SJ,Labuzan SA,Sethi I,Harris TH

25-5698-82 was used in Flow cytometry/Cell sorting to reveal that CNS lymphatic drainage supports the development of peripheral T cell responses against T. gondii but remains dispensable for immune protection of the brain.

Wed Dec 21 00:00:00 EST 2022

Inhibiting Type I Arginine Methyltransferase Activity Promotes T Cell-Mediated Antitumor Immune Responses.

Cancer immunology research

Fedoriw A,Shi L,O'Brien S,Smitheman KN,Wang Y,Hou J,Sherk C,Rajapurkar S,Laraio J,Williams LJ,Xu C,Han G,Feng Q,Bedford MT,Wang L,Barbash O,Kruger RG,Hwu P,Mohammad HP,Peng W

25-5698-82 was used in Flow cytometry/Cell sorting to indicate that type I PRMT inhibition exhibits immunomodulatory properties and synergizes with immune checkpoint blockade to induce durable antitumor responses in a T cell-dependent manner, suggesting that type I PRMT inhibition can potentiate an antitumor immunity in refractory settings.

Fri Apr 01 00:00:00 EDT 2022

IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence.

Nature communications

Chiang HY,Lu HH,Sudhakar JN,Chen YW,Shih NS,Weng YT,Shui JW

25-5698-82 was used in Flow Cytometry to conclude that IL-18 in return regulates Stat3-mediated anti-microbial response in Paneth cells, Akt-Tcf4-triggered expansion of Lgr5+ stem cells to facilitate tissue repair, and AIEC clearance by promoting IFNγ+ T cells.

Tue Feb 15 00:00:00 EST 2022

Functional virus-specific memory T cells survey glioblastoma.

Cancer immunology, immunotherapy : CII

Ning J,Gavil NV,Wu S,Wijeyesinghe S,Weyu E,Ma J,Li M,Grigore FN,Dhawan S,Skorput AGJ,Musial SC,Chen CC,Masopust D,Rosato PC

25-5698-82 was used in Flow cytometry/Cell sorting to indicate that virus-specific memory T cells are a significant part of the glioblastoma immune microenvironment and may be leveraged to promote anti-tumoral immunity.

Mon Aug 01 00:00:00 EDT 2022

Ubiquitin-specific peptidase 18 regulates the differentiation and function of Treg cells.

Genes & diseases

Yang L,Jing Y,Kang D,Jiang P,Li N,Zhou X,Chen Y,Westerberg LS,Liu C

25-5698-82 was used in Flow Cytometry to identify the role of USP18 in Tregs differentiation and its suppressive function.

Sat May 01 00:00:00 EDT 2021

MIF but not MIF-2 recruits inflammatory macrophages in an experimental polymicrobial sepsis model.

The Journal of clinical investigation

Tilstam PV,Schulte W,Holowka T,Kim BS,Nouws J,Sauler M,Piecychna M,Pantouris G,Lolis E,Leng L,Bernhagen J,Fingerle-Rowson G,Bucala R

25-5698-82 was used in flow cytometry to study Mif-/- and Mif-2-/- mice to polymicrobial sepsis and observed a survival benefit with Mif but not Mif-2 deficiency.

Wed Dec 01 00:00:00 EST 2021

Ddb1 Is Essential for the Expansion of CD4⁺ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.

Frontiers in immunology

Yang L,Chen W,Li L,Xiao Y,Fan S,Zhang Q,Xia T,Li M,Hong Y,Zhao T,Li Q,Liu WH,Xiao N

25-5698-82 was used in Flow Cytometry to understand the molecular mechanism underlying the generation of follicular helper T cells.

Mon Dec 20 00:00:00 EST 2021

Obesity results in adipose tissue T cell exhaustion.

JCI insight

Porsche CE,Delproposto JB,Geletka L,O'Rourke R,Lumeng CN

25-5698-82 was used in Flow Cytometry to assess adipose tissue T cells activation and inflammatory potential within mouse and human stromal vascular fraction.

Thu Apr 22 00:00:00 EDT 2021

Gut-Innervating Nociceptor Neurons Regulate Peyer's Patch Microfold Cells and SFB Levels to Mediate Salmonella Host Defense.

Cell

Lai NY,Musser MA,Pinho-Ribeiro FA,Baral P,Jacobson A,Ma P,Potts DE,Chen Z,Paik D,Soualhi S,Yan Y,Misra A,Goldstein K,Lagomarsino VN,Nordstrom A,Sivanathan KN,Wallrapp A,Kuchroo VK,Nowarski R,Starnbach MN,Shi H,Surana NK,An D,Wu C,Huh JR,Rao M,Chiu IM

25-5698-82 was used in Flow Cytometry to reveal a major role for nociceptor neurons in sensing and defending against enteric pathogens.

Thu Jan 09 00:00:00 EST 2020

EBF1-deficient bone marrow stroma elicits persistent changes in HSC potential.

Nature immunology

Derecka M,Herman JS,Cauchy P,Ramamoorthy S,Lupar E,Grün D,Grosschedl R

25-5698-82 was used in Flow Cytometry to investigate how transcriptional changes in bone marrow (BM) mesenchymal stromal cells result in long-lasting effects on hematopoietic stem cells.

Sun Mar 01 00:00:00 EST 2020

Microphthalmia transcription factor expression contributes to bone marrow failure in Fanconi anemia.

The Journal of clinical investigation

Oppezzo A,Bourseguin J,Renaud E,Pawlikowska P,Rosselli F

25-5698 was used in Flow cytometry/Cell sorting to the impact of the constitutive activation of stress signaling pathways on hematopoietic stem cell maintenance and function in Fanconi anemia.

Mon Mar 02 00:00:00 EST 2020

SRC-3 Functions as a Coactivator of T-bet by Regulating the Maturation and Antitumor Activity of Natural Killer Cells.

Cancer immunology research

Hu M,Lu Y,Qi Y,Zhang Z,Wang S,Xu Y,Chen F,Tang Y,Chen S,Chen M,Du C,Shen M,Wang F,Su Y,Deng Y,Wang J

25-5698-82 was used in Flow Cytometry to study the role of SRC-3 as a coactivator of T-bet in natural killer-cell biology and indicate that targeting SRC-3 may be a promising strategy to increase the tumor surveillance function of natural killer cells.

Tue Sep 01 00:00:00 EDT 2020

CX3CR1 positively regulates BCR signaling coupled with cell metabolism via negatively controlling actin remodeling.

Cellular and molecular life sciences : CMLS

Li N,Jiang P,Chen A,Luo X,Jing Y,Yang L,Kang D,Chen Q,Liu J,Chang J,Jellusova J,Miller H,Westerberg L,Wang CY,Gong Q,Liu C

25-5698-82 was used in Flow Cytometry to study the effect of CX3CR1 deficiency on BCR signalling and B cell differentiation using CX3CR1 KO mice.

Sun Nov 01 00:00:00 EDT 2020

Nanoparticle-Coupled Topical Methotrexate Can Normalize Immune Responses and Induce Tissue Remodeling in Psoriasis.

The Journal of investigative dermatology

Özcan A,Sahin D,Impellizzieri D,Nguyen TT,Hafner J,Yawalkar N,Kurzbach D,Tan G,Akdis CA,Nilsson J,Boyman O,Kolios AGA

25-5698 was used in Flow cytometry/Cell sorting to show that MTX coupled with gold nanoparticles (GNPs) demonstrates superior antiinflammatory efficacy than MTX alone in an imiquimod-induced mouse model, significantly reducing γδ T cells, CD4+ T cells, and neutrophils.

Fri May 01 00:00:00 EDT 2020

Diversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells.

Nature communications

Lucas B,White AJ,Cosway EJ,Parnell SM,James KD,Jones ND,Ohigashi I,Takahama Y,Jenkinson WE,Anderson G

25-5698 was used in Flow cytometry/Cell sorting to study the mechanism that controls the development of αβ T cell lineages in the thymus.

Mon May 04 00:00:00 EDT 2020

Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells.

Nature communications

Harsha Krovi S,Zhang J,Michaels-Foster MJ,Brunetti T,Loh L,Scott-Browne J,Gapin L

25-5698 was used in Flow cytometry/Cell sorting to highlight the common requirements for the development of innate-like T cells with a focus on how Hivep3 impacts the maturation of these lymphocytes.

Mon Dec 07 00:00:00 EST 2020

Landscape of Exhausted Virus-Specific CD8 T Cells in Chronic LCMV Infection.

Cell reports

Sandu I,Cerletti D,Oetiker N,Borsa M,Wagen F,Spadafora I,Welten SPM,Stolz U,Oxenius A,Claassen M

25-5698-82 was used in Flow Cytometry to reveal that exhausted cells are heterogeneous, adopt organ-specific transcriptomic profiles, and can be divided into five main functional subpopulations: advanced exhaustion, effector-like, intermediate, proliferating, or memory-like.

Tue Aug 25 00:00:00 EDT 2020

Lumenal Galectin-9-Lamp2 interaction regulates lysosome and autophagy to prevent pathogenesis in the intestine and pancreas.

Nature communications

Sudhakar JN,Lu HH,Chiang HY,Suen CS,Hwang MJ,Wu SY,Shen CN,Chang YM,Li FA,Liu FT,Shui JW

25-5698 was used in Flow cytometry/Cell sorting to show in gut epithelial cells, galectin-9 is enriched in lysosomes and predominantly binds to lysosome-associated membrane protein 2 (Lamp2) in a Asn(N)-glycan dependent manner.

Thu Aug 27 00:00:00 EDT 2020

A Subset of Skin Macrophages Contributes to the Surveillance and Regeneration of Local Nerves.

Immunity

Kolter J,Feuerstein R,Zeis P,Hagemeyer N,Paterson N,d'Errico P,Baasch S,Amann L,Masuda T,Lösslein A,Gharun K,Meyer-Luehmann M,Waskow C,Franzke CW,Grün D,Lämmermann T,Prinz M,Henneke P

25-5698 was used in Flow cytometry/Cell sorting to delineate the postnatal development of dermal macrophages and their differentiation into subsets by adapting single-cell transcriptomics, fate mapping, and imaging.

Tue Jun 18 00:00:00 EDT 2019

Calcitonin Gene-Related Peptide Negatively Regulates Alarmin-Driven Type 2 Innate Lymphoid Cell Responses.

Immunity

Wallrapp A,Burkett PR,Riesenfeld SJ,Kim SJ,Christian E,Abdulnour RE,Thakore PI,Schnell A,Lambden C,Herbst RH,Khan P,Tsujikawa K,Xavier RJ,Chiu IM,Levy BD,Regev A,Kuchroo VK

25-5698-82 was used in Flow Cytometry to show that ILC2s expressed both the neuropeptide calcitonin gene-related peptide and its receptor.

Tue Oct 15 00:00:00 EDT 2019

Causes and Consequences of miR-150-5p Dysregulation in Myasthenia Gravis.

Frontiers in immunology

Cron MA,Maillard S,Truffault F,Gualeni AV,Gloghini A,Fadel E,Guihaire J,Behin A,Berrih-Aknin S,Le Panse R

25-5698 was used in Flow cytometry/Cell sorting to show miR-150-5p dysregulation can increase death of CD4+ and CD8+ T cells and induce overexpression of pro-apoptotic genes normally targeted by miR-150.

Tue Aug 04 00:00:00 EDT 2020

The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.

Immunity

Wang D,Diao H,Getzler AJ,Rogal W,Frederick MA,Milner J,Yu B,Crotty S,Goldrath AW,Pipkin ME

25-5698 was used in Flow cytometry/Cell sorting to conclude that Runx3 governs chromatin accessibility during TCR stimulation and enforces the memory CTL developmental program.

Tue Apr 17 00:00:00 EDT 2018

Ablation of Transcription Factor IRF4 Promotes Transplant Acceptance by Driving Allogenic CD4⁺ T Cell Dysfunction.

Immunity

Wu J,Zhang H,Shi X,Xiao X,Fan Y,Minze LJ,Wang J,Ghobrial RM,Xia J,Sciammas R,Li XC,Chen W

25-5698-82 was used in Flow Cytometry to demonstrate that interferon regulatory factor 4 was a key transcriptional determinant controlling T cell responses during transplantation.

Tue Dec 19 00:00:00 EST 2017

Intestinal helminth infection induces highly functional resident memory CD4⁺ T cells in mice.

European journal of immunology

Steinfelder S,Rausch S,Michael D,Kühl AA,Hartmann S

Wed Feb 01 00:00:00 EST 2017

Revisiting the Road Map of Medullary Thymic Epithelial Cell Differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Michel C,Miller CN,Küchler R,Brors B,Anderson MS,Kyewski B,Pinto S

25-5698 was used in Flow cytometry/Cell sorting to introduce the lectin Tetragonolobus purpureas agglutinin as a novel cell surface marker that allows for such delineation.

Wed Nov 15 00:00:00 EST 2017

CD8⁺ T cells drive autoimmune hematopoietic stem cell dysfunction and bone marrow failure.

Journal of autoimmunity

Gravano DM,Al-Kuhlani M,Davini D,Sanders PD,Manilay JO,Hoyer KK

25-5698 was used in Flow cytometry/Cell sorting to investigate whether CD8+ T cells play a non-redundant role in driving bone marrow failure.

Thu Dec 01 00:00:00 EST 2016

Macrophages transfer antigens to dendritic cells by releasing exosomes containing dead-cell-associated antigens partially through a ceramide-dependent pathway to enhance CD4(+) T-cell responses.

Immunology

Xu Y,Liu Y,Yang C,Kang L,Wang M,Hu J,He H,Song W,Tang H

25-5698 was used in Flow cytometry/Cell sorting to identify a novel pathway of cross-talk between macrophages and dendritic cells.

Sat Oct 01 00:00:00 EDT 2016

SWEF Proteins Distinctly Control Maintenance and Differentiation of Hematopoietic Stem Cells.

PloS one

Ripich T,Chacón-Martínez CA,Fischer L,Pernis A,Kiessling N,Garbe AI,Jessberger R

25-5698 was used in Flow cytometry/Cell sorting to investigate the role of SWAP-70 and DEF6 in hematopoietic stem and precursor cells.

Mon Jul 31 00:00:00 EDT 2017

Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment.

Nature communications

Spanier JA,Frederick DR,Taylor JJ,Heffernan JR,Kotov DI,Martinov T,Osum KC,Ruggiero JL,Rust BJ,Landry SJ,Jenkins MK,McLachlan JB,Fife BT

25-5698 was used in Flow cytometry/Cell sorting to develop a novel approach for the generation and screening of peptide:MHCII reactive mAbs that exploits the use of recombinant peptide:MHC monomers as immunogens.

Mon Jun 13 00:00:00 EDT 2016

IL-2/α-IL-2 Complex Treatment Cannot Be Substituted for the Adoptive Transfer of Regulatory T cells to Promote Bone Marrow Engraftment.

PloS one

Mahr B,Unger L,Hock K,Pilat N,Baranyi U,Schwarz C,Maschke S,Farkas AM,Wekerle T

25-5698 was used in Flow cytometry/Cell sorting to investigate IL-2 complex treatment as a replacement for regulatory T cell adoptive transfer to promote bone marrow engraftment.

Thu Jun 30 00:00:00 EDT 2016

Interdependent IL-7 and IFN-γ signalling in T-cell controls tumour eradication by combined α-CTLA-4+α-PD-1 therapy.

Nature communications

Shi LZ,Fu T,Guan B,Chen J,Blando JM,Allison JP,Xiong L,Subudhi SK,Gao J,Sharma P

25-5698 was used in Flow cytometry/Cell sorting to study how tumour eradication can be controlled by interdependent IL-7 and IFN-γ signalling in T-cells.

Mon Aug 08 00:00:00 EDT 2016

Epidermal keratinocytes initiate wound healing and pro-inflammatory immune responses following percutaneous schistosome infection.

International journal for parasitology

Bourke CD,Prendergast CT,Sanin DE,Oulton TE,Hall RJ,Mountford AP

25-5698 was used in Flow cytometry/Cell sorting to indicate that S.mansoni cercariae and their excretory/secretory products act directly upon epidermal keratinocytes.

Sun Mar 01 00:00:00 EST 2015

IL-1R signaling in dendritic cells replaces pattern-recognition receptors in promoting CD8⁺ T cell responses to influenza A virus.

Nature immunology

Pang IK,Ichinohe T,Iwasaki A

Fri Mar 01 00:00:00 EST 2013

Primate B-1 cells generate antigen-specific B cell responses to T cell-independent type 2 antigens.

Journal of immunology (Baltimore, Md. : 1950)

Yammani RD,Haas KM

Mon Apr 01 00:00:00 EDT 2013

Myeloid cells expressing VEGF and arginase-1 following uptake of damaged retinal pigment epithelium suggests potential mechanism that drives the onset of choroidal angiogenesis in mice.

PloS one

Liu J,Copland DA,Horie S,Wu WK,Chen M,Xu Y,Paul Morgan B,Mack M,Xu H,Nicholson LB,Dick AD

Thu Jan 15 00:00:00 EST 2015

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; unnamed protein product

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Gene Aliases: D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105

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UniProt ID: (Human) P46013, (Mouse) E9PVX6

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Entrez Gene ID: (Dog) 100686578, (Human) 4288, (Cynomolgus monkey) 102135895, (Rat) 291234, (Mouse) 17345

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Function(s)

protein binding ATP binding protein C-terminus binding poly(A) RNA binding molecular_function

Process(es)

DNA metabolic process meiotic nuclear division cell proliferation response to organic cyclic compound hyaluronan metabolic process organ regeneration cellular response to heat hepatocyte proliferation cholangiocyte proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ki-67 Monoclonal Antibody (SolA15), PE-Cyanine7, eBioscience™

Ki-67 Antibody (25-5698-82) in Flow

Product Details

25-5698-82

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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