Invitrogen

Ki-67 Monoclonal Antibody (SolA15), PerCP-eFluor™ 710, eBioscience™

FIGURE: 1 / 57

Ki-67 Antibody (46-5698-82) in Flow

Intracellular staining of BALB/c splenocytes unstimulated (left) or stimulated for 2 days with immobilized Anti-Mouse CD3 Functional Grade (Product # 16-0031-82) (right) with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.03 µg of Anti-Mouse/Rat Ki-67 PerCP-eFluor® 710 using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.

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Product Details

46-5698-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.06 µg/test

View 61 publications 61 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SolA15

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11040981

Product Specific Information

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).

The SolA15 antibody also recognizes human, non-human primate and canine Ki-67.

Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This SolA15 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (Product # 00-5521-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

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Thakker P,Ariana A,Hajjar S,Cai D,Rijal D,Sad S

46-5698-82 was used in Flow cytometry/Cell sorting to reveal the temporal impact of XIAP in promoting the fitness of activated CD8 T cells through cell extrinsic and intrinsic mechanisms and provide a mechanistic explanation of the phenotype observed in XLP-2 patients.

Thu Jun 01 00:00:00 EDT 2023

Transformation of primary murine peritoneal mast cells by constitutive KIT activation is accompanied by loss of Cdkn2a/Arf expression.

Frontiers in immunology

Capellmann S,Sonntag R,Schüler H,Meurer SK,Gan L,Kauffmann M,Horn K,Königs-Werner H,Weiskirchen R,Liedtke C,Huber M

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Wed Apr 19 00:00:00 EDT 2023

B-cell antigen receptor expression and phosphatidylinositol 3-kinase signaling regulate genesis and maintenance of mouse chronic lymphocytic leukemia.

Haematologica

Schmid VK,Khadour A,Ahmed N,Brandl C,Nitschke L,Rajewsky K,Jumaa H,Hobeika E

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Antibody-drug conjugates harboring a kinesin spindle protein inhibitor with immunostimulatory properties.

Oncoimmunology

Sommer A,Berndt S,Lerchen HG,Forveille S,Sauvat A,Mumberg D,Kroemer G,Kepp O

46-5698-82 was used in Flow Cytometry to conclude that the antineoplastic effects of TWEAKR-KSPi-ADCs can partly be attributed to its ICD-stimulatory properties.

Fri Apr 08 00:00:00 EDT 2022

CD8 memory precursor cell generation is a continuous process.

iScience

Todorov H,Prieux M,Laubreton D,Bouvier M,Wang S,de Bernard S,Arpin C,Cannoodt R,Saelens W,Bonnaffoux A,Gandrillon O,Crauste F,Saeys Y,Marvel J

46-5698-82 was used in flow cytometry to study the generation of memory precursor cells following an acute infection by analyzing single-cell RNA-seq data that contained CD8 T cells collected during the postinfection expansion phase.

Fri Sep 16 00:00:00 EDT 2022

USP2 promotes experimental colitis and bacterial infections by inhibiting the proliferation of myeloid cells and remodeling the extracellular matrix network.

Cell insight

An R,Wang P,Guo H,Liuyu T,Zhong B,Zhang ZD

46-5698-82 was used in Flow cytometry/Cell sorting to report a susceptible role of ubiquitin-specific protease 2 (USP2) in experimental colitis and bacterial infections.

Mon Aug 01 00:00:00 EDT 2022

Macrophage LAMTOR1 Deficiency Prevents Dietary Obesity and Insulin Resistance Through Inflammation-Induced Energy Expenditure.

Frontiers in cell and developmental biology

Ying L,Zhang M,Ma X,Si Y,Li X,Su J,Yin J,Bao Y

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

HOXA11 plays critical roles in disease progression and response to cytarabine in AML.

Oncology reports

Fu JF,Shih LY,Yen TH

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Mutant ASXL1 induces age-related expansion of phenotypic hematopoietic stem cells through activation of Akt/mTOR pathway.

Nature communications

Fujino T,Goyama S,Sugiura Y,Inoue D,Asada S,Yamasaki S,Matsumoto A,Yamaguchi K,Isobe Y,Tsuchiya A,Shikata S,Sato N,Morinaga H,Fukuyama T,Tanaka Y,Fukushima T,Takeda R,Yamamoto K,Honda H,Nishimura EK,Furukawa Y,Shibata T,Abdel-Wahab O,Suematsu M,Kitamura T

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Tue Mar 23 00:00:00 EDT 2021

An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia.

Cell reports

Suzuki S,McCarrey JR,Hermann BP

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2021

Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

Cell reports

Budhu S,Giese R,Gupta A,Fitzgerald K,Zappasodi R,Schad S,Hirschhorn D,Campesato LF,De Henau O,Gigoux M,Liu C,Mazo G,Deng L,Barker CA,Wolchok JD,Merghoub T

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Shikonin Derivatives from Onsoma visianii Decrease Expression of Phosphorylated STAT3 in Leukemia Cells and Exert Antitumor Activity.

Nutrients

Todorovic Z,Milovanovic J,Arsenijevic D,Vukovic N,Vukic M,Arsenijevic A,Djurdjevic P,Milovanovic M,Arsenijevic N

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Wed Mar 31 00:00:00 EDT 2021

Regulatory T cells promote cancer immune-escape through integrin αvβ8-mediated TGF-β activation.

Nature communications

Lainé A,Labiad O,Hernandez-Vargas H,This S,Sanlaville A,Léon S,Dalle S,Sheppard D,Travis MA,Paidassi H,Marie JC

46-5698-82 was used in Flow Cytometry to investigate whether TGFβ activation within the tumor depends on different cell types and mechanisms than its production.

Thu Oct 28 00:00:00 EDT 2021

TNF-α-induced alterations in stromal progenitors enhance leukemic stem cell growth via CXCR2 signaling.

Cell reports

Agarwal P,Li H,Choi K,Hueneman K,He J,Welner RS,Starczynowski DT,Bhatia R

46-5698-82 was used in Flow Cytometry to find that TNF-α-mediated alterations in CML BM stromal niches enhance support of LSC maintenance and growth via CXCL1-CXCR2 signaling and that CXCR2 inhibition effectively depletes CML LSCs.

Tue Jul 13 00:00:00 EDT 2021

CD177, a specific marker of neutrophil activation, is associated with coronavirus disease 2019 severity and death.

iScience

Lévy Y,Wiedemann A,Hejblum BP,Durand M,Lefebvre C,Surénaud M,Lacabaratz C,Perreau M,Foucat E,Déchenaud M,Tisserand P,Blengio F,Hivert B,Gauthier M,Cervantes-Gonzalez M,Bachelet D,Laouénan C,Bouadma L,Timsit JF,Yazdanpanah Y,Pantaleo G,Hocini H,Thiébaut R

46-5698-82 was used in Flow Cytometry to perform cell phenotypic, serum, and RNA sequencing gene expression analyses in severe hospitalized patients , to investigate serum CD177 as a specific neutrophil activation maker for coronavirus disease.

Fri Jul 23 00:00:00 EDT 2021

Mycobacterium tuberculosis-infected alveolar epithelial cells modulate dendritic cell function through the HIF-1α-NOS2 axis.

Journal of leukocyte biology

Rodrigues TS,Alvarez ARP,Gembre AF,Forni MFPAD,de Melo BMS,Alves Filho JCF,Câmara NOS,Bonato VLD

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Chemosensitization of prostate cancer stem cells in mice by angiogenin and plexin-B2 inhibitors.

Communications biology

Li S,Goncalves KA,Lyu B,Yuan L,Hu GF

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Wed Jan 15 00:00:00 EST 2020

Follicular Regulatory T Cells Can Access the Germinal Center Independently of CXCR5.

Cell reports

Vanderleyden I,Fra-Bido SC,Innocentin S,Stebegg M,Okkenhaug H,Evans-Bailey N,Pierson W,Denton AE,Linterman MA

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Tue Jan 21 00:00:00 EST 2020

CD103⁺ cDC1 and endogenous CD8⁺ T cells are necessary for improved CD40L-overexpressing CAR T cell antitumor function.

Nature communications

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Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Wed Dec 02 00:00:00 EST 2020

Treatment of mice with a ligand binding blocking anti-CD28 monoclonal antibody improves healing after myocardial infarction.

PloS one

Gladow N,Hollmann C,Ramos G,Frantz S,Kerkau T,Beyersdorf N,Hofmann U

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Cardiac fibroblast proliferation rates and collagen expression mature early and are unaltered with advancing age.

JCI insight

Wu R,Ma F,Tosevska A,Farrell C,Pellegrini M,Deb A

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Thu Dec 17 00:00:00 EST 2020

Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow.

Nature communications

Riedel R,Addo R,Ferreira-Gomes M,Heinz GA,Heinrich F,Kummer J,Greiff V,Schulz D,Klaeden C,Cornelis R,Menzel U,Kröger S,Stervbo U,Köhler R,Haftmann C,Kühnel S,Lehmann K,Maschmeyer P,McGrath M,Naundorf S,Hahne S,Sercan-Alp Ö,Siracusa F,Stefanowski J,Weber M,Westendorf K,Zimmermann J,Hauser AE,Reddy ST,Durek P,Chang HD,Mashreghi MF,Radbruch A

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Fri May 22 00:00:00 EDT 2020

RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures.

ImmunoHorizons

Li D,Bradley T,Cain DW,Pedroza-Pacheco I,Aggelakopoulou M,Parks R,Barr M,Xia SM,Scearce R,Bowman C,Stevens G,Newman A,Hora B,Chen Y,Riebe K,Wang Y,Sempowski G,Saunders KO,Borrow P,Haynes BF

46-5698 was used in Flow cytometry/Cell sorting to study the role of Rab11Fip5 in immune responses.

Tue Nov 10 00:00:00 EST 2020

Metabolite-Sensing Receptor Ffar2 Regulates Colonic Group 3 Innate Lymphoid Cells and Gut Immunity.

Immunity

Chun E,Lavoie S,Fonseca-Pereira D,Bae S,Michaud M,Hoveyda HR,Fraser GL,Gallini Comeau CA,Glickman JN,Fuller MH,Layden BT,Garrett WS

46-5698 was used in Flow cytometry/Cell sorting to demonstrate group 3 innate lymphoid cells are regulated by the metabolite-sensing receptor Ffar2.

Tue Nov 19 00:00:00 EST 2019

FCRL5⁺ Memory B Cells Exhibit Robust Recall Responses.

Cell reports

Kim CC,Baccarella AM,Bayat A,Pepper M,Fontana MF

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Tue Apr 30 00:00:00 EDT 2019

Pulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer.

Cell reports

Choi H,Deng J,Li S,Silk T,Dong L,Brea EJ,Houghton S,Redmond D,Zhong H,Boiarsky J,Akbay EA,Smith PD,Merghoub T,Wong KK,Wolchok JD

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Tue Apr 16 00:00:00 EDT 2019

Keap1-Nrf2 System Plays an Important Role in Invariant Natural Killer T Cell Development and Homeostasis.

Cell reports

Pyaram K,Kumar A,Kim YH,Noel S,Reddy SP,Rabb H,Chang CH

46-5698 was used in Flow cytometry/Cell sorting to report that T cellspecific deletion of Keap1 results in defective development and reduced numbers of invariant natural killer T (NKT) cells in the thymus and the peripheral organs in a cell-intrinsic manner.

Tue Apr 16 00:00:00 EDT 2019

Keap1-Nrf2 System Plays an Important Role in Invariant Natural Killer T Cell Development and Homeostasis.

Cell reports

Pyaram K,Kumar A,Kim YH,Noel S,Reddy SP,Rabb H,Chang CH

Tue Apr 16 00:00:00 EDT 2019

Ets1 suppresses atopic dermatitis by suppressing pathogenic T cell responses.

JCI insight

Lee CG,Kwon HK,Kang H,Kim Y,Nam JH,Won YH,Park S,Kim T,Kang K,Rudra D,Jun CD,Park ZY,Im SH

46-5698 was used in Flow cytometry/Cell sorting to reveal a protective role of Ets1 in restricting pathogenic Th cell responses and suggest a potential therapeutic target for atopic dermatitis (AD) treatment.

Thu Mar 07 00:00:00 EST 2019

Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.

Nature medicine

Maryanovich M,Zahalka AH,Pierce H,Pinho S,Nakahara F,Asada N,Wei Q,Wang X,Ciero P,Xu J,Leftin A,Frenette PS

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Modulation of Myelopoiesis Progenitors Is an Integral Component of Trained Immunity.

Cell

Mitroulis I,Ruppova K,Wang B,Chen LS,Grzybek M,Grinenko T,Eugster A,Troullinaki M,Palladini A,Kourtzelis I,Chatzigeorgiou A,Schlitzer A,Beyer M,Joosten LAB,Isermann B,Lesche M,Petzold A,Simons K,Henry I,Dahl A,Schultze JL,Wielockx B,Zamboni N,Mirtschink P,Coskun Ü,Hajishengallis G,Netea MG,Chavakis T

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Thu Jan 11 00:00:00 EST 2018

A Metabolite-Triggered Tuft Cell-ILC2 Circuit Drives Small Intestinal Remodeling.

Cell

Schneider C,O'Leary CE,von Moltke J,Liang HE,Ang QY,Turnbaugh PJ,Radhakrishnan S,Pellizzon M,Ma A,Locksley RM

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Thu Jul 12 00:00:00 EDT 2018

Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.

Cancer cell

Arce Vargas F,Furness AJS,Litchfield K,Joshi K,Rosenthal R,Ghorani E,Solomon I,Lesko MH,Ruef N,Roddie C,Henry JY,Spain L,Ben Aissa A,Georgiou A,Wong YNS,Smith M,Strauss D,Hayes A,Nicol D,O'Brien T,Mårtensson L,Ljungars A,Teige I,Frendéus B,Pule M,Marafioti T,Gore M,Larkin J,Turajlic S,Swanton C,Peggs KS,Quezada SA

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Mon Apr 09 00:00:00 EDT 2018

Nongenetic origins of cell-to-cell variability in B lymphocyte proliferation.

Proceedings of the National Academy of Sciences of the United States of America

Mitchell S,Roy K,Zangle TA,Hoffmann A

46-5698 was used in Flow cytometry/Cell sorting to characterise the role of molecular noise in determining phenotype heterogeneity.

Tue Mar 20 00:00:00 EDT 2018

Myeloid β-Catenin Deficiency Exacerbates Atherosclerosis in Low-Density Lipoprotein Receptor-Deficient Mice.

Arteriosclerosis, thrombosis, and vascular biology

Wang F,Liu Z,Park SH,Gwag T,Lu W,Ma M,Sui Y,Zhou C

46-5698 was used in Immunohistochemistry to investigate the impact of Β-catenin expression on macrophage functions and atherosclerosis development.

Sun Jul 01 00:00:00 EDT 2018

MicroRNA-126 deficiency enhanced the activation and function of CD4⁺ T cells by elevating IRS-1 pathway.

Clinical and experimental immunology

Chu F,Hu Y,Zhou Y,Guo M,Lu J,Zheng W,Xu H,Zhao J,Xu L

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Lymphotoxin α fine-tunes T cell clonal deletion by regulating thymic entry of antigen-presenting cells.

Nature communications

Lopes N,Charaix J,Cédile O,Sergé A,Irla M

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Wed Mar 28 00:00:00 EDT 2018

Pax6 Lengthens G1 Phase and Decreases Oscillating Cdk6 Levels in Murine Embryonic Cortical Progenitors.

Frontiers in cellular neuroscience

Mi D,Manuel M,Huang YT,Mason JO,Price DJ

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Thu Mar 04 00:00:00 EST 2021

The organic ester O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride attenuates murine breast cancer growth and metastasis.

Oncotarget

Jurisevic M,Arsenijevic A,Pantic J,Gajovic N,Milovanovic J,Milovanovic M,Poljarevic J,Sabo T,Vojvodic D,Radosavljevic GD,Arsenijevic N

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Fri Jun 15 00:00:00 EDT 2018

ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases.

Frontiers in immunology

Glal D,Sudhakar JN,Lu HH,Liu MC,Chiang HY,Liu YC,Cheng CF,Shui JW

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Thu Sep 26 00:00:00 EDT 2019

Stem cell factor is selectively secreted by arterial endothelial cells in bone marrow.

Nature communications

Xu C,Gao X,Wei Q,Nakahara F,Zimmerman SE,Mar J,Frenette PS

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Fri Jun 22 00:00:00 EDT 2018

A somatic mutation in erythro-myeloid progenitors causes neurodegenerative disease.

Nature

Mass E,Jacome-Galarza CE,Blank T,Lazarov T,Durham BH,Ozkaya N,Pastore A,Schwabenland M,Chung YR,Rosenblum MK,Prinz M,Abdel-Wahab O,Geissmann F

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Thu Sep 21 00:00:00 EDT 2017

Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.

Nature

Hérault A,Binnewies M,Leong S,Calero-Nieto FJ,Zhang SY,Kang YA,Wang X,Pietras EM,Chu SH,Barry-Holson K,Armstrong S,Göttgens B,Passegué E

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Thu Apr 06 00:00:00 EDT 2017

De Novo Epigenetic Programs Inhibit PD-1 Blockade-Mediated T Cell Rejuvenation.

Cell

Ghoneim HE,Fan Y,Moustaki A,Abdelsamed HA,Dash P,Dogra P,Carter R,Awad W,Neale G,Thomas PG,Youngblood B

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Thu Jun 29 00:00:00 EDT 2017

White Adipose Tissue Is a Reservoir for Memory T Cells and Promotes Protective Memory Responses to Infection.

Immunity

Han SJ,Glatman Zaretsky A,Andrade-Oliveira V,Collins N,Dzutsev A,Shaik J,Morais da Fonseca D,Harrison OJ,Tamoutounour S,Byrd AL,Smelkinson M,Bouladoux N,Bliska JB,Brenchley JM,Brodsky IE,Belkaid Y

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Tue Dec 19 00:00:00 EST 2017

Differential cytokine contributions of perivascular haematopoietic stem cell niches.

Nature cell biology

Asada N,Kunisaki Y,Pierce H,Wang Z,Fernandez NF,Birbrair A,Ma'ayan A,Frenette PS

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Mutations in 5-methylcytosine oxidase TET2 and RhoA cooperatively disrupt T cell homeostasis.

The Journal of clinical investigation

Zang S,Li J,Yang H,Zeng H,Han W,Zhang J,Lee M,Moczygemba M,Isgandarova S,Yang Y,Zhou Y,Rao A,You MJ,Sun D,Huang Y

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Mon Jun 05 00:00:00 EDT 2017

Identification of Nascent Memory CD8 T Cells and Modeling of Their Ontogeny.

Cell systems

Crauste F,Mafille J,Boucinha L,Djebali S,Gandrillon O,Marvel J,Arpin C

46-5698-82 was used in Flow Cytometry to identify Bcl2 and Mki67 as a marker combination that enables the tracking of nascent memory cells within the effector phase.

Wed Mar 22 00:00:00 EDT 2017

1810011o10 Rik Inhibits the Antitumor Effect of Intratumoral CD8⁺ T Cells through Suppression of Notch2 Pathway in a Murine Hepatocellular Carcinoma Model.

Frontiers in immunology

Dai K,Huang L,Huang YB,Chen ZB,Yang LH,Jiang YA

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Neonatal pancreatic pericytes support β-cell proliferation.

Molecular metabolism

Epshtein A,Rachi E,Sakhneny L,Mizrachi S,Baer D,Landsman L

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

Foxp3 and Toll-like receptor signaling balance T_reg cell anabolic metabolism for suppression.

Nature immunology

Gerriets VA,Kishton RJ,Johnson MO,Cohen S,Siska PJ,Nichols AG,Warmoes MO,de Cubas AA,MacIver NJ,Locasale JW,Turka LA,Wells AD,Rathmell JC

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Oligodendrocyte death results in immune-mediated CNS demyelination.

Nature neuroscience

Traka M,Podojil JR,McCarthy DP,Miller SD,Popko B

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Schwann cell proliferation and differentiation that is induced by ferulic acid through MEK1/ERK1/2 signalling promotes peripheral nerve remyelination following crush injury in rats.

Experimental and therapeutic medicine

Zhu X,Li K,Guo X,Wang J,Xiang Y

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Growth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression.

Oncoimmunology

Chen L,Yi X,Goswami S,Ahn YH,Roybal JD,Yang Y,Diao L,Peng D,Peng D,Fradette JJ,Wang J,Byers LA,Kurie JM,Ullrich SE,Qin FX,Gibbons DL

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Immune response modulation by Galectin-1 in a transgenic model of neuroblastoma.

Oncoimmunology

Büchel G,Schulte JH,Harrison L,Batzke K,Schüller U,Hansen W,Schramm A

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

The stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas.

Oncoimmunology

Sonner JK,Deumelandt K,Ott M,Thomé CM,Rauschenbach KJ,Schulz S,Munteanu B,Mohapatra S,Adam I,Hofer AC,Feuerer M,Opitz CA,Hopf C,Wick W,Platten M

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

The cell proliferation antigen Ki-67 organises heterochromatin.

eLife

Sobecki M,Mrouj K,Camasses A,Parisis N,Nicolas E,Llères D,Gerbe F,Prieto S,Krasinska L,David A,Eguren M,Birling MC,Urbach S,Hem S,Déjardin J,Malumbres M,Jay P,Dulic V,Lafontaine DLj,Feil R,Fisher D

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Mon Mar 07 00:00:00 EST 2016

The cell proliferation antigen Ki-67 organises heterochromatin.

eLife

Sobecki M,Mrouj K,Camasses A,Parisis N,Nicolas E,Llères D,Gerbe F,Prieto S,Krasinska L,David A,Eguren M,Birling MC,Urbach S,Hem S,Déjardin J,Malumbres M,Jay P,Dulic V,Lafontaine DLj,Feil R,Fisher D

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Mon Mar 07 00:00:00 EST 2016

Mammary Stem Cells and Tumor-Initiating Cells Are More Resistant to Apoptosis and Exhibit Increased DNA Repair Activity in Response to DNA Damage.

Stem cell reports

Chang CH,Zhang M,Rajapakshe K,Coarfa C,Edwards D,Huang S,Rosen JM

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2015

Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.

Nature communications

Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX

Published figure using Ki-67 monoclonal antibody (Product # 46-5698-82) in Flow Cytometry

Tue Oct 28 00:00:00 EDT 2014

IL-1R signaling in dendritic cells replaces pattern-recognition receptors in promoting CD8⁺ T cell responses to influenza A virus.

Nature immunology

Pang IK,Ichinohe T,Iwasaki A

Fri Mar 01 00:00:00 EST 2013

Primate B-1 cells generate antigen-specific B cell responses to T cell-independent type 2 antigens.

Journal of immunology (Baltimore, Md. : 1950)

Yammani RD,Haas KM

Mon Apr 01 00:00:00 EDT 2013

Small intestine inflammation in Roquin-mutant and Roquin-deficient mice.

PloS one

Schaefer JS,Montufar-Solis D,Nakra N,Vigneswaran N,Klein JR

Tue Sep 03 00:00:00 EDT 2013

Myeloid cells expressing VEGF and arginase-1 following uptake of damaged retinal pigment epithelium suggests potential mechanism that drives the onset of choroidal angiogenesis in mice.

PloS one

Liu J,Copland DA,Horie S,Wu WK,Chen M,Xu Y,Paul Morgan B,Mack M,Xu H,Nicholson LB,Dick AD

Thu Jan 15 00:00:00 EST 2015

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; unnamed protein product

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Gene Aliases: D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105

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UniProt ID: (Human) P46013, (Mouse) E9PVX6

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Entrez Gene ID: (Dog) 100686578, (Human) 4288, (Cynomolgus monkey) 102135895, (Rat) 291234, (Mouse) 17345

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Function(s)

protein binding ATP binding protein C-terminus binding poly(A) RNA binding molecular_function

Process(es)

DNA metabolic process meiotic nuclear division cell proliferation response to organic cyclic compound hyaluronan metabolic process organ regeneration cellular response to heat hepatocyte proliferation cholangiocyte proliferation

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Ki-67 Monoclonal Antibody (SolA15), PerCP-eFluor™ 710, eBioscience™

Ki-67 Antibody (46-5698-82) in Flow

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46-5698-82

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