Invitrogen

IL-2 Monoclonal Antibody (MQ1-17H12), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

13 Published Figures

20 References

View all (144) IL-2 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 15

IL-2 Antibody (25-7029-42) in Flow

Normal human peripheral blood cells were unstimulated (left) or stimulated (right) with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03). Cells were intracellularly stained with Anti-Human CD4 APC (Product # 17-0047-42) and Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) or Anti-Human IL-2 PE-Cyanine7. Viable cells, as determined by Fixable Viability Dye eFluor® 780 (Product # 65-0865-14), in the lymphocyte gate were used for analysis.

Product Image Gallery

IL-2 Antibody — Intracellular staining of stimulated human peripheral blood cells. As expected based on known expression patterns, IL-2 clone MQ1-17H12 stains a subset of CD4+ T cells only after stimulation (top) and does not stain CD19+ B cells regardless of stimulation (top and bottom). Details: Normal human peripheral blood cells were cultured in the presence of Protein Transport Inhibitors (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD19 (clone SJ25C1), CD4 (clone RPA-T4), and IL-2 (clone MQ1-17H12). Cells in the CD19+ (blue histogram) or CD4+ (purple histogram) gates were used for analysis. {TM}

Product Details

25-7029-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 20 publications 20 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MQ1-17H12

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573518

Product Specific Information

Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.

Applications Reported: This MQ1-17H12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MQ1-17H12 antibody has been pre-titrated and tested by intracellular staining and flwo cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Nanobody-derived bispecific CAR-T cell therapy enhances the anti-tumor efficacy of T cell lymphoma treatment.

Molecular therapy oncolytics

Xia B,Lin K,Wang X,Chen F,Zhou M,Li Y,Lin Y,Qiao Y,Li R,Zhang W,He X,Zou F,Li L,Lu L,Chen C,Li W,Zhang H,Liu B

Published figure using IL-2 monoclonal antibody (Product # 25-7029-42) in Flow Cytometry

Thu Sep 21 00:00:00 EDT 2023

PTPN22 R620W gene editing in T cells enhances low-avidity TCR responses.

eLife

Anderson W,Barahmand-Pour-Whitman F,Linsley PS,Cerosaletti K,Buckner JH,Rawlings DJ

25-7029-42 was used in Flow cytometry/Cell sorting to suggest that, in primary human T cells, PTPN22 rs2476601 contributes to autoimmunity risk by permitting increased TCR signaling and activation in mildly self-reactive T cells, thereby potentially expanding the self-reactive T cell pool and skewing this population toward an inflammatory phenotype.

Fri Mar 24 00:00:00 EDT 2023

Dynamics of spike-and nucleocapsid specific immunity during long-term follow-up and vaccination of SARS-CoV-2 convalescents.

Nature communications

Koerber N,Priller A,Yazici S,Bauer T,Cheng CC,Mijočević H,Wintersteller H,Jeske S,Vogel E,Feuerherd M,Tinnefeld K,Winter C,Ruland J,Gerhard M,Haller B,Christa C,Zelger O,Roggendorf H,Halle M,Erber J,Lingor P,Keppler O,Zehn D,Protzer U,Knolle PA

Published figure using IL-2 monoclonal antibody (Product # 25-7029-42) in Flow Cytometry

Mon Jan 10 00:00:00 EST 2022

Enhanced antibody responses in fully vaccinated individuals against pan-SARS-CoV-2 variants following Omicron breakthrough infection.

Cell reports. Medicine

Jeong HW,Kim SM,Jung MK,Noh JY,Yoo JS,Kim EH,Kim YI,Yu K,Jang SG,Gil J,Casel MA,Rare R,Choi JH,Kim HS,Kim JH,Um J,Kim C,Kim Y,Chin BS,Jung S,Choi JY,Song KH,Kim YD,Park JS,Song JY,Shin EC,Choi YK

Published figure using IL-2 monoclonal antibody (Product # 25-7029-42) in Flow Cytometry

Tue Oct 18 00:00:00 EDT 2022

CRISPR/Cas9-Mediated Insertion of HIV Long Terminal Repeat within BACH2 Promotes Expansion of T Regulatory-like Cells.

Journal of immunology (Baltimore, Md. : 1950)

Christian ML,Dapp MJ,Scharffenberger SC,Jones H,Song C,Frenkel LM,Krumm A,Mullins JI,Rawlings DJ

25-7029-42 was used in Flow cytometry/Cell sorting to model the effects of HIV integration within BACH2 Integration of the HIV long terminal repeat (LTR) and major splice donor increased BACH2 mRNA and protein levels, altered gene expression, and promoted selective outgrowth of an activated, proliferative, and T regulatory-like cell population.

Fri Apr 01 00:00:00 EDT 2022

Decreased Frequencies of Gamma/Delta T Cells Expressing Th1/Th17 Cytokine, Cytotoxic, and Immune Markers in Latent Tuberculosis-Diabetes/Pre-Diabetes Comorbidity.

Frontiers in cellular and infection microbiology

Kathamuthu GR,Kumar NP,Moideen K,Menon PA,Babu S

Published figure using IL-2 monoclonal antibody (Product # 25-7029-42) in Flow Cytometry

Thu Nov 25 00:00:00 EST 2021

Platelets stimulate programmed death-ligand 1 expression by cancer cells: Inhibition by anti-platelet drugs.

Journal of thrombosis and haemostasis : JTH

Asgari A,Lesyk G,Poitras E,Govindasamy N,Terry K,To R,Back V,Rudzinski JK,Lewis JD,Jurasz P

Published figure using IL-2 monoclonal antibody (Product # 25-7029-42) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca²⁺ entry in T cells.

Cell reports

Wu B,Woo JS,Vila P,Jew M,Leung J,Sun Z,Srikanth S,Gwack Y

Published figure using IL-2 monoclonal antibody (Product # 25-7029-42) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIV.

Nature communications

Alrubayyi A,Gea-Mallorquí E,Touizer E,Hameiri-Bowen D,Kopycinski J,Charlton B,Fisher-Pearson N,Muir L,Rosa A,Roustan C,Earl C,Cherepanov P,Pellegrino P,Waters L,Burns F,Kinloch S,Dong T,Dorrell L,Rowland-Jones S,McCoy LE,Peppa D

Published figure using IL-2 monoclonal antibody (Product # 25-7029-42) in Flow Cytometry

Tue Oct 05 00:00:00 EDT 2021

BNT162b vaccines protect rhesus macaques from SARS-CoV-2.

Nature

Vogel AB,Kanevsky I,Che Y,Swanson KA,Muik A,Vormehr M,Kranz LM,Walzer KC,Hein S,Güler A,Loschko J,Maddur MS,Ota-Setlik A,Tompkins K,Cole J,Lui BG,Ziegenhals T,Plaschke A,Eisel D,Dany SC,Fesser S,Erbar S,Bates F,Schneider D,Jesionek B,Sänger B,Wallisch AK,Feuchter Y,Junginger H,Krumm SA,Heinen AP,Adams-Quack P,Schlereth J,Schille S,Kröner C,de la Caridad Güimil Garcia R,Hiller T,Fischer L,Sellers RS,Choudhary S,Gonzalez O,Vascotto F,Gutman MR,Fontenot JA,Hall-Ursone S,Brasky K,Griffor MC,Han S,Su AAH,Lees JA,Nedoma NL,Mashalidis EH,Sahasrabudhe PV,Tan CY,Pavliakova D,Singh G,Fontes-Garfias C,Pride M,Scully IL,Ciolino T,Obregon J,Gazi M,Carrion R,Alfson KJ,Kalina WV,Kaushal D,Shi PY,Klamp T,Rosenbaum C,Kuhn AN,Türeci Ö,Dormitzer PR,Jansen KU,Sahin U

25-7029-42 was used in flow cytometry to report the preclinical development of two vaccine candidates (BNT162b1 and BNT162b2) that contain nucleoside-modified messenger RNA that encodes immunogens derived from the spike glycoprotein (S) of SARS-CoV-2, formulated in lipid nanoparticles.

Thu Apr 01 00:00:00 EDT 2021

BNT162b vaccines protect rhesus macaques from SARS-CoV-2.

Nature

Thu Apr 01 00:00:00 EDT 2021

BNT162b vaccines protect rhesus macaques from SARS-CoV-2.

Nature

Thu Apr 01 00:00:00 EDT 2021

Rejuvenating Effector/Exhausted CAR T Cells to Stem Cell Memory-Like CAR T Cells By Resting Them in the Presence of CXCL12 and the NOTCH Ligand.

Cancer research communications

Ando M,Kondo T,Tomisato W,Ito M,Shichino S,Srirat T,Mise-Omata S,Nakagawara K,Yoshimura A

Published figure using IL-2 monoclonal antibody (Product # 25-7029-42) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Senolytic CAR T cells reverse senescence-associated pathologies.

Nature

Amor C,Feucht J,Leibold J,Ho YJ,Zhu C,Alonso-Curbelo D,Mansilla-Soto J,Boyer JA,Li X,Giavridis T,Kulick A,Houlihan S,Peerschke E,Friedman SL,Ponomarev V,Piersigilli A,Sadelain M,Lowe SW

25-7029 was used in Flow cytometry/Cell sorting to establish the therapeutic potential of senolytic CAR T cells for senescence-associated diseases.

Wed Jul 01 00:00:00 EDT 2020

CD19-targeted CAR regulatory T cells suppress B cell pathology without GvHD.

JCI insight

Imura Y,Ando M,Kondo T,Ito M,Yoshimura A

25-7029 was used in Flow cytometry/Cell sorting to suggest that CD19-CAR regulatory T cells can treat autoantibody-mediated autoimmune diseases by suppressing B cell pathology.

Thu Jul 23 00:00:00 EDT 2020

CRISPR-Mediated Base Conversion Allows Discriminatory Depletion of Endogenous T Cell Receptors for Enhanced Synthetic Immunity.

Molecular therapy. Methods & clinical development

Preece R,Pavesi A,Gkazi SA,Stegmann KA,Georgiadis C,Tan ZM,Aw JYJ,Maini MK,Bertoletti A,Qasim W

Published figure using IL-2 monoclonal antibody (Product # 25-7029-42) in Flow Cytometry

Fri Dec 11 00:00:00 EST 2020

Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors.

Nature communications

Webber BR,Lonetree CL,Kluesner MG,Johnson MJ,Pomeroy EJ,Diers MD,Lahr WS,Draper GM,Slipek NJ,Smeester BA,Lovendahl KN,McElroy AN,Gordon WR,Osborn MJ,Moriarity BS

25-7029-42 was used in Flow Cytometry to demonstrate that base editors can mediate highly efficient multiplex gene disruption with minimal double-strand break induction.

Tue Nov 19 00:00:00 EST 2019

Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors.

Nature communications

Webber BR,Lonetree CL,Kluesner MG,Johnson MJ,Pomeroy EJ,Diers MD,Lahr WS,Draper GM,Slipek NJ,Smeester BA,Lovendahl KN,McElroy AN,Gordon WR,Osborn MJ,Moriarity BS

25-7029-42 was used in Flow Cytometry to demonstrate that base editors can mediate highly efficient multiplex gene disruption with minimal double-strand break induction.

Tue Nov 19 00:00:00 EST 2019

Inflammatory Bowel Disease Types Differ in Markers of Inflammation, Gut Barrier and in Specific Anti-Bacterial Response.

Cells

Coufal S,Galanova N,Bajer L,Gajdarova Z,Schierova D,Jiraskova Zakostelska Z,Kostovcikova K,Jackova Z,Stehlikova Z,Drastich P,Tlaskalova-Hogenova H,Kverka M

25-7029-42 was used in Flow Cytometry to prove the importance of the gut barrier function and immune response to commensal bacteria and point at the specific differences in pathogenesis of PSC-IBD, UC and CD.

Sat Jul 13 00:00:00 EDT 2019

Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.

The Journal of experimental medicine

Carpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C

Published figure using IL-2 monoclonal antibody (Product # 25-7029-42) in Flow Cytometry

Mon Apr 02 00:00:00 EDT 2018

A novel dual-cytokine-antibody fusion protein for the treatment of CD38-positive malignancies.

Protein engineering, design & selection : PEDS

De Luca R,Kachel P,Kropivsek K,Snijder B,Manz MG,Neri D

Published figure using IL-2 monoclonal antibody (Product # 25-7029-42) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

A Quantitative Analysis of Complexity of Human Pathogen-Specific CD4 T Cell Responses in Healthy M. tuberculosis Infected South Africans.

PLoS pathogens

Lindestam Arlehamn CS,McKinney DM,Carpenter C,Paul S,Rozot V,Makgotlho E,Gregg Y,van Rooyen M,Ernst JD,Hatherill M,Hanekom WA,Peters B,Scriba TJ,Sette A

25-7029 was used in Flow cytometry/Cell sorting to estimate the breadth of T cell responses for the first time in the context of an infection and human population setting.

Fri Jul 01 00:00:00 EDT 2016

PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer.

Cancer immunology research

Severson JJ,Serracino HS,Mateescu V,Raeburn CD,McIntyre RC,Sams SB,Haugen BR,French JD

25-7029 was used in Flow cytometry/Cell sorting to determine that the release of PD-1 and Tim-3, and the inhibition of Tregs, may encourage tumour elimination in metastatic differentiated thyroid cancer.

Mon Jun 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: aldesleukin; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: IL-2; IL2; lymphokine; TCGF

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UniProt ID: (Human) P60568

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Entrez Gene ID: (Human) 3558

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Function(s)

Ras guanyl-nucleotide exchange factor activity cytokine activity interleukin-2 receptor binding growth factor activity kinase activator activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding

Process(es)

Performance Guarantee

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IL-2 Monoclonal Antibody (MQ1-17H12), PE-Cyanine7, eBioscience™

IL-2 Antibody (25-7029-42) in Flow

Product Details

25-7029-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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