Invitrogen

IL-2 Monoclonal Antibody (MQ1-17H12), PerCP-eFluor™ 710, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

12 Published Figures

16 References

View all (144) IL-2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 14

IL-2 Antibody (46-7029-42) in Flow

Normal human peripheral blood cells were unstimulated (left) or stimulated with PMA/ionomycin in the presence of Brefeldin A for 5 hours (right), then intracellularly stained with Anti-Human CD4 APC (Product # 17-0049-42) and Anti-Human IL-2 PerCP-eFluor® 710. Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

IL-2 Antibody — Intracellular staining of stimulated human peripheral blood cells. As expected based on known expression patterns, IL-2 clone MQ1-17H12 stains a subset of CD4+ T cells only after stimulation (top) and does not stain CD19+ B cells regardless of stimulation (top and bottom). Details: Normal human peripheral blood cells were cultured in the presence of Protein Transport Inhibitors (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD19 (clone SJ25C1), CD4 (clone RPA-T4), and IL-2 (clone MQ1-17H12). Cells in the CD19+ (blue histogram) or CD4+ (purple histogram) gates were used for analysis. {TM}

Product Details

46-7029-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MQ1-17H12

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1834419

Product Specific Information

Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.

Applications Reported: This MQ1-17H12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MQ1-17H12 antibody has been pre-titrated and tested by flow cytometric analysis of activated human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Nanobody-derived bispecific CAR-T cell therapy enhances the anti-tumor efficacy of T cell lymphoma treatment.

Molecular therapy oncolytics

Xia B,Lin K,Wang X,Chen F,Zhou M,Li Y,Lin Y,Qiao Y,Li R,Zhang W,He X,Zou F,Li L,Lu L,Chen C,Li W,Zhang H,Liu B

Published figure using IL-2 monoclonal antibody (Product # 46-7029-42) in Flow Cytometry

Thu Sep 21 00:00:00 EDT 2023

Pre-existing polymerase-specific T cells expand in abortive seronegative SARS-CoV-2.

Nature

Swadling L,Diniz MO,Schmidt NM,Amin OE,Chandran A,Shaw E,Pade C,Gibbons JM,Le Bert N,Tan AT,Jeffery-Smith A,Tan CCS,Tham CYL,Kucykowicz S,Aidoo-Micah G,Rosenheim J,Davies J,Johnson M,Jensen MP,Joy G,McCoy LE,Valdes AM,Chain BM,Goldblatt D,Altmann DM,Boyton RJ,Manisty C,Treibel TA,Moon JC,van Dorp L,Balloux F,McKnight Á,Noursadeghi M,Bertoletti A,Maini MK

46-7029-42 was used in Flow Cytometry to study whether pre-existing memory T cell responses, with cross-protective potential against SARS-CoV-2, would expand in vivo to support rapid viral control, aborting infection.

Sat Jan 01 00:00:00 EST 2022

Dynamics of spike-and nucleocapsid specific immunity during long-term follow-up and vaccination of SARS-CoV-2 convalescents.

Nature communications

Koerber N,Priller A,Yazici S,Bauer T,Cheng CC,Mijočević H,Wintersteller H,Jeske S,Vogel E,Feuerherd M,Tinnefeld K,Winter C,Ruland J,Gerhard M,Haller B,Christa C,Zelger O,Roggendorf H,Halle M,Erber J,Lingor P,Keppler O,Zehn D,Protzer U,Knolle PA

Published figure using IL-2 monoclonal antibody (Product # 46-7029-42) in Flow Cytometry

Mon Jan 10 00:00:00 EST 2022

Enhanced antibody responses in fully vaccinated individuals against pan-SARS-CoV-2 variants following Omicron breakthrough infection.

Cell reports. Medicine

Jeong HW,Kim SM,Jung MK,Noh JY,Yoo JS,Kim EH,Kim YI,Yu K,Jang SG,Gil J,Casel MA,Rare R,Choi JH,Kim HS,Kim JH,Um J,Kim C,Kim Y,Chin BS,Jung S,Choi JY,Song KH,Kim YD,Park JS,Song JY,Shin EC,Choi YK

Published figure using IL-2 monoclonal antibody (Product # 46-7029-42) in Flow Cytometry

Tue Oct 18 00:00:00 EDT 2022

Decreased Frequencies of Gamma/Delta T Cells Expressing Th1/Th17 Cytokine, Cytotoxic, and Immune Markers in Latent Tuberculosis-Diabetes/Pre-Diabetes Comorbidity.

Frontiers in cellular and infection microbiology

Kathamuthu GR,Kumar NP,Moideen K,Menon PA,Babu S

Published figure using IL-2 monoclonal antibody (Product # 46-7029-42) in Flow Cytometry

Thu Nov 25 00:00:00 EST 2021

Platelets stimulate programmed death-ligand 1 expression by cancer cells: Inhibition by anti-platelet drugs.

Journal of thrombosis and haemostasis : JTH

Asgari A,Lesyk G,Poitras E,Govindasamy N,Terry K,To R,Back V,Rudzinski JK,Lewis JD,Jurasz P

Published figure using IL-2 monoclonal antibody (Product # 46-7029-42) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca²⁺ entry in T cells.

Cell reports

Wu B,Woo JS,Vila P,Jew M,Leung J,Sun Z,Srikanth S,Gwack Y

Published figure using IL-2 monoclonal antibody (Product # 46-7029-42) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIV.

Nature communications

Alrubayyi A,Gea-Mallorquí E,Touizer E,Hameiri-Bowen D,Kopycinski J,Charlton B,Fisher-Pearson N,Muir L,Rosa A,Roustan C,Earl C,Cherepanov P,Pellegrino P,Waters L,Burns F,Kinloch S,Dong T,Dorrell L,Rowland-Jones S,McCoy LE,Peppa D

Published figure using IL-2 monoclonal antibody (Product # 46-7029-42) in Flow Cytometry

Tue Oct 05 00:00:00 EDT 2021

Rejuvenating Effector/Exhausted CAR T Cells to Stem Cell Memory-Like CAR T Cells By Resting Them in the Presence of CXCL12 and the NOTCH Ligand.

Cancer research communications

Ando M,Kondo T,Tomisato W,Ito M,Shichino S,Srirat T,Mise-Omata S,Nakagawara K,Yoshimura A

Published figure using IL-2 monoclonal antibody (Product # 46-7029-42) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

CAR T Cells Targeting MISIIR for the Treatment of Ovarian Cancer and Other Gynecologic Malignancies.

Molecular therapy : the journal of the American Society of Gene Therapy

Rodriguez-Garcia A,Sharma P,Poussin M,Boesteanu AC,Minutolo NG,Gitto SB,Omran DK,Robinson MK,Adams GP,Simpkins F,Powell DJ

46-7029 was used in Flow cytometry/Cell sorting to indicate that MISIIR targeting for the treatment of ovarian cancer and other gynecologic malignancies is achievable using CAR technology.

Wed Feb 05 00:00:00 EST 2020

Measuring Chimeric Antigen Receptor T Cells (CAR T Cells) Activation by Coupling Intracellular Cytokine Staining with Flow Cytometry.

Methods in molecular biology (Clifton, N.J.)

Xu C,Yin Y

46-7029-42 was used in Flow Cytometry /test) to describe coupling of intracellular cytokine staining and multicolor flow cytometry to measure CAR T cells activation upon antigen stimulation.

Wed Jan 27 00:00:00 EST 2021

CRISPR-Mediated Base Conversion Allows Discriminatory Depletion of Endogenous T Cell Receptors for Enhanced Synthetic Immunity.

Molecular therapy. Methods & clinical development

Preece R,Pavesi A,Gkazi SA,Stegmann KA,Georgiadis C,Tan ZM,Aw JYJ,Maini MK,Bertoletti A,Qasim W

46-7029 was used in Flow cytometry/Cell sorting to report lentiviral delivery of a hepatitis B-virus (HBV)-specific recombinant T cell receptor (rTCR) and a linked CRISPR single-guide RNA for simultaneous disruption of endogenous TCRs (eTCRs) when combined with transient cytosine deamination.

Fri Dec 11 00:00:00 EST 2020

CRISPR-Mediated Base Conversion Allows Discriminatory Depletion of Endogenous T Cell Receptors for Enhanced Synthetic Immunity.

Molecular therapy. Methods & clinical development

Preece R,Pavesi A,Gkazi SA,Stegmann KA,Georgiadis C,Tan ZM,Aw JYJ,Maini MK,Bertoletti A,Qasim W

Fri Dec 11 00:00:00 EST 2020

Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.

The Journal of experimental medicine

Carpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C

Published figure using IL-2 monoclonal antibody (Product # 46-7029-42) in Flow Cytometry

Mon Apr 02 00:00:00 EDT 2018

Lactobacillus rhamnosus GG-Derived Soluble Mediators Modulate Adaptive Immune Cells.

Frontiers in immunology

Ludwig IS,Broere F,Manurung S,Lambers TT,van der Zee R,van Eden W

46-7029 was used in Flow cytometry/Cell sorting to determine the role of Lactobacillus rhamosus soluble mediators in adaptive immune responses.

Tue Sep 26 00:00:00 EDT 2023

A novel dual-cytokine-antibody fusion protein for the treatment of CD38-positive malignancies.

Protein engineering, design & selection : PEDS

De Luca R,Kachel P,Kropivsek K,Snijder B,Manz MG,Neri D

Published figure using IL-2 monoclonal antibody (Product # 46-7029-42) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

Genetic Architecture of Adaptive Immune System Identifies Key Immune Regulators.

Cell reports

Lagou V,Garcia-Perez JE,Smets I,Van Horebeek L,Vandebergh M,Chen L,Mallants K,Prezzemolo T,Hilven K,Humblet-Baron S,Moisse M,Van Damme P,Boeckxstaens G,Bowness P,Dubois B,Dooley J,Liston A,Goris A

46-7029-42 was used in Flow Cytometry to identify eight genome-wide significant associations explaining 6%-20% of variance in immune system regulation.

Tue Oct 16 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: aldesleukin; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: IL-2; IL2; lymphokine; TCGF

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UniProt ID: (Human) P60568

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Entrez Gene ID: (Human) 3558

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Function(s)

Ras guanyl-nucleotide exchange factor activity cytokine activity interleukin-2 receptor binding growth factor activity kinase activator activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-2 Monoclonal Antibody (MQ1-17H12), PerCP-eFluor™ 710, eBioscience™

IL-2 Antibody (46-7029-42) in Flow

Product Details

46-7029-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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