Invitrogen

IL-4 Monoclonal Antibody (11B11), PE-Cyanine7, eBioscience™

38 Published Figures

37 References

View all (109) IL-4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (38)

FIGURE: 1 / 39

IL-4 Antibody (25-7041-82) in Flow

Mouse Th2-polarized splenocytes were unstimulated (left) or stimulated (right) with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03). The cells were intracellularly stained with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Anti-Mouse IL-4 PE-Cyanine7 using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

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Product Details

25-7041-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 37 publications 37 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

11B11

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573520

Product Specific Information

Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.

Applications Reported: This 11B11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 11B11 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This antibody can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Subconjunctival injection of human umbilical cord mesenchymal stem cells alleviates experimental allergic conjunctivitis via regulating T cell response.

Stem cell research & therapy

Li D,Ou Q,Shen Q,Lu MM,Xu JY,Jin C,Gao F,Wang J,Zhang J,Zhang J,Li J,Lu L,Xu GT,Tian H

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Mon Oct 02 00:00:00 EDT 2023

Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challenge.

Biomaterials

Sun S,Li E,Zhao G,Tang J,Zuo Q,Cai L,Xu C,Sui C,Ou Y,Liu C,Li H,Ding Y,Li C,Lu D,Zhang W,Luo P,Cheng P,Gao Y,Tu C,Pitard B,Rosenecker J,Wang B,Liu Y,Zou Q,Guan S

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

25-7041-82 was used in Flow cytometry/Cell sorting to demonstrate for the first time that CalD is a novel anti-inflammatory natural compound for allergic asthma that modulates AM function.

Sat Apr 01 00:00:00 EDT 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

25-7041-82 was used in Flow cytometry/Cell sorting to demonstrate for the first time that CalD is a novel anti-inflammatory natural compound for allergic asthma that modulates AM function.

Sat Apr 01 00:00:00 EDT 2023

Dendritic cell Piezo1 directs the differentiation of T_H1 and T_reg cells in cancer.

eLife

Wang Y,Yang H,Jia A,Wang Y,Yang Q,Dong Y,Hou Y,Cao Y,Dong L,Bi Y,Liu G

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Mon Aug 22 00:00:00 EDT 2022

NKT cells adopt a glutamine-addicted phenotype to regulate their homeostasis and function.

Cell reports

Kumar A,Yarosz EL,Andren A,Zhang L,Lyssiotis CA,Chang CH

25-7041-82 was used in Flow cytometry/Cell sorting to highlight a distinct metabolic requirement of NKT cells compared with CD4 T cells, which may have therapeutic implications in the treatment of certain nutrient-restricted diseases.

Tue Oct 25 00:00:00 EDT 2022

Romidepsin (FK228) regulates the expression of the immune checkpoint ligand PD-L1 and suppresses cellular immune functions in colon cancer.

Cancer immunology, immunotherapy : CII

Shi Y,Fu Y,Zhang X,Zhao G,Yao Y,Guo Y,Ma G,Bai S,Li H

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Romidepsin (FK228) regulates the expression of the immune checkpoint ligand PD-L1 and suppresses cellular immune functions in colon cancer.

Cancer immunology, immunotherapy : CII

Shi Y,Fu Y,Zhang X,Zhao G,Yao Y,Guo Y,Ma G,Bai S,Li H

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Fine particulate matter induces airway inflammation by disturbing the balance between Th1/Th2 and regulation of GATA3 and Runx3 expression in BALB/c mice.

Molecular medicine reports

Pang L,Yu P,Liu X,Fan Y,Shi Y,Zou S

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells.

Oncology letters

Chen J,Ding Y,Huang F,Lan R,Wang Z,Huang W,Chen R,Wu B,Fu L,Yang Y,Liu J,Hong J,Zhang W,Zhang L

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Features of T-cell subset composition in a D-galactose-induced senescence mouse model.

Experimental animals

Kawata K,Suzuki T,Ozawa K,Sekiguchi M

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Fri Aug 06 00:00:00 EDT 2021

Exosome-mediated mRNA delivery in vivo is safe and can be used to induce SARS-CoV-2 immunity.

The Journal of biological chemistry

Tsai SJ,Atai NA,Cacciottolo M,Nice J,Salehi A,Guo C,Sedgwick A,Kanagavelu S,Gould SJ

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

RSV infection-elicited high MMP-12-producing macrophages exacerbate allergic airway inflammation with neutrophil infiltration.

iScience

Makino A,Shibata T,Nagayasu M,Hosoya I,Nishimura T,Nakano C,Nagata K,Ito T,Takahashi Y,Nakamura S

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Fri Oct 22 00:00:00 EDT 2021

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Tue Jan 11 00:00:00 EST 2022

Smek1 deficiency exacerbates experimental autoimmune encephalomyelitis by activating proinflammatory microglia and suppressing the IDO1-AhR pathway.

Journal of neuroinflammation

Duan RN,Yang CL,Du T,Liu A,Wang AR,Sun WJ,Li X,Li JX,Yan CZ,Liu QJ

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Mon Jun 28 00:00:00 EDT 2021

SPARC regulation of PMN clearance protects from pristane-induced lupus and rheumatoid arthritis.

iScience

Sangaletti S,Botti L,Gulino A,Lecis D,Bassani B,Portararo P,Milani M,Cancila V,De Cecco L,Dugo M,Tripodo C,Colombo MP

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry.

STAR protocols

Skordos I,Demeyer A,Beyaert R

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

TIRC7 inhibits Th1 cells by upregulating the expression of CTLA‑4 and STAT3 in mice with acute graft‑versus‑host disease.

Oncology reports

Zhu F,Qiu T,Zhu S,Zhao K,Chen C,Qiao J,Pan B,Yan Z,Chen W,Liu Q,Wu Q,Cao J,Sang W,Zeng L,Sun H,Li Z,Xu K

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Diversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells.

Nature communications

Lucas B,White AJ,Cosway EJ,Parnell SM,James KD,Jones ND,Ohigashi I,Takahama Y,Jenkinson WE,Anderson G

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Mon May 04 00:00:00 EDT 2020

High Glucose Intake Exacerbates Autoimmunity through Reactive-Oxygen-Species-Mediated TGF-β Cytokine Activation.

Immunity

Zhang D,Jin W,Wu R,Li J,Park SA,Tu E,Zanvit P,Xu J,Liu O,Cain A,Chen W

25-7041-82 was used in Flow Cytometry to reveal a previously unrecognized mechanism underlying the adverse effects of high glucose intake in the pathogenesis of autoimmunity and inflammation.

Tue Oct 15 00:00:00 EDT 2019

IL-31 plays dual roles in lung inflammation in an OVA-induced murine asthma model.

Biology open

Huang J,Yue H,Jiang T,Gao J,Shi Y,Shi B,Wu X,Gou X

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Tue Jan 15 00:00:00 EST 2019

Keap1-Nrf2 System Plays an Important Role in Invariant Natural Killer T Cell Development and Homeostasis.

Cell reports

Pyaram K,Kumar A,Kim YH,Noel S,Reddy SP,Rabb H,Chang CH

25-7041 was used in Flow cytometry/Cell sorting to report that T cellspecific deletion of Keap1 results in defective development and reduced numbers of invariant natural killer T (NKT) cells in the thymus and the peripheral organs in a cell-intrinsic manner.

Tue Apr 16 00:00:00 EDT 2019

Keap1-Nrf2 System Plays an Important Role in Invariant Natural Killer T Cell Development and Homeostasis.

Cell reports

Pyaram K,Kumar A,Kim YH,Noel S,Reddy SP,Rabb H,Chang CH

Tue Apr 16 00:00:00 EDT 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

c-Maf controls immune responses by regulating disease-specific gene networks and repressing IL-2 in CD4⁺ T cells.

Nature immunology

Gabryšová L,Alvarez-Martinez M,Luisier R,Cox LS,Sodenkamp J,Hosking C,Pérez-Mazliah D,Whicher C,Kannan Y,Potempa K,Wu X,Bhaw L,Wende H,Sieweke MH,Elgar G,Wilson M,Briscoe J,Metzis V,Langhorne J,Luscombe NM,O'Garra A

25-7041 was used in Flow cytometry/Cell sorting to study the role of c-Maf as a regulator of the expression of cytokine-encoding genes.

Tue May 01 00:00:00 EDT 2018

MicroRNA-126 deficiency enhanced the activation and function of CD4⁺ T cells by elevating IRS-1 pathway.

Clinical and experimental immunology

Chu F,Hu Y,Zhou Y,Guo M,Lu J,Zheng W,Xu H,Zhao J,Xu L

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Baicalin Downregulates RLRs Signaling Pathway to Control Influenza A Virus Infection and Improve the Prognosis.

Evidence-based complementary and alternative medicine : eCAM

Pang P,Zheng K,Wu S,Xu H,Deng L,Shi Y,Chen X

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation.

Genome medicine

Pramanik J,Chen X,Kar G,Henriksson J,Gomes T,Park JE,Natarajan K,Meyer KB,Miao Z,McKenzie ANJ,Mahata B,Teichmann SA

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Wed Oct 24 00:00:00 EDT 2018

Trichinella spiralis Infection Mitigates Collagen-Induced Arthritis via Programmed Death 1-Mediated Immunomodulation.

Frontiers in immunology

Cheng Y,Zhu X,Wang X,Zhuang Q,Huyan X,Sun X,Huang J,Zhan B,Zhu X

Mon Jan 28 00:00:00 EST 2019

Group 2 innate lymphoid cells protect lung endothelial cells from pyroptosis in sepsis.

Cell death & disease

Lai D,Tang J,Chen L,Fan EK,Scott MJ,Li Y,Billiar TR,Wilson MA,Fang X,Shu Q,Fan J

25-7041 was used in Flow cytometry/Cell sorting to describe the function of ILC2 in the regulation of lung inflammation by protecting lung endothelial cell from pyroptosis.

Tue Mar 06 00:00:00 EST 2018

Critical role of caspase-8-mediated IL-1 signaling in promoting Th2 responses during asthma pathogenesis.

Mucosal immunology

Qi X,Gurung P,Malireddi RK,Karmaus PW,Sharma D,Vogel P,Chi H,Green DR,Kanneganti TD

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Intestinal helminth infection induces highly functional resident memory CD4⁺ T cells in mice.

European journal of immunology

Steinfelder S,Rausch S,Michael D,Kühl AA,Hartmann S

25-7041 was used in Flow cytometry/Cell sorting to show that nonlymphoid compartments can serve as reservoirs for Th2 memory cells, and furthermore that innate effector function of Th2 memory cells is restricted to CD4⁺ memory T cells residing in the peritoneal cavity.

Wed Feb 01 00:00:00 EST 2017

Reactive Oxygen Species Regulate the Inflammatory Function of NKT Cells through Promyelocytic Leukemia Zinc Finger.

Journal of immunology (Baltimore, Md. : 1950)

Kim YH,Kumar A,Chang CH,Pyaram K

25-7041 was used in Flow cytometry/Cell sorting to investigate the role of reactive oxygen species in natural killer T cell function.

Wed Nov 15 00:00:00 EST 2017

Toxoplasma Co-infection Prevents Th2 Differentiation and Leads to a Helminth-Specific Th1 Response.

Frontiers in cellular and infection microbiology

Ahmed N,French T,Rausch S,Kühl A,Hemminger K,Dunay IR,Steinfelder S,Hartmann S

25-7041 was used in Flow cytometry/Cell sorting to conclude a previous T. gondii infection limits a helminth-specific Th2 immune response while promoting a shift toward a Th1-type immune response.

Mon Apr 02 00:00:00 EDT 2018

IKKβ acts as a tumor suppressor in cancer-associated fibroblasts during intestinal tumorigenesis.

The Journal of experimental medicine

Pallangyo CK,Ziegler PK,Greten FR

25-7041 was used in Flow cytometry/Cell sorting to highlight an unexpected tumor suppressive function of IKKβ/NF-κB in CAFs linked to HGF release.

Mon Dec 14 00:00:00 EST 2015

Inadequate activation of the HBsAg-specific Th cells by APCs leads to hyporesponsiveness to HBsAg vaccine in B10.S mice.

Human vaccines & immunotherapeutics

Li X,Xu J,Lv Z,Wang J,Sun S,Zhu W,Wang B,He R,Qu D

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Tue Apr 19 00:00:00 EDT 2016

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Mon Jan 05 00:00:00 EST 2015

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

Interleukin-22 as a molecular adjuvant facilitates IL-17-producing CD8+ T cell responses against a HBV DNA vaccine in mice.

Human vaccines & immunotherapeutics

Wu B,Zou Q,Hu Y,Wang B

Published figure using IL-4 monoclonal antibody (Product # 25-7041-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

Inchoate CD8+ T cell responses in neonatal mice permit influenza-induced persistent pulmonary dysfunction.

Journal of immunology (Baltimore, Md. : 1950)

You D,Ripple M,Balakrishna S,Troxclair D,Sandquist D,Ding L,Ahlert TA,Cormier SA

25-7041 was used in Flow cytometry/Cell sorting to establish a neonatal mouse model of influenza infection to elucidate the mechanisms that induce severe pulmonary disease in infants.

Mon Sep 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

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Gene Aliases: BSF-1; Il-4; Il4

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UniProt ID: (Mouse) P07750

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Entrez Gene ID: (Mouse) 16189

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Function(s)

cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-4 Monoclonal Antibody (11B11), PE-Cyanine7, eBioscience™

IL-4 Antibody (25-7041-82) in Flow

Product Details

25-7041-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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