Invitrogen

4-Hydroxynonenal Monoclonal Antibody (12F7)

8 Published Figures

16 References

View all (10) 4-Hydroxynonenal antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (8)

4-Hydroxynonenal Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

4-Hydroxynonenal Antibody (MA5-27570) in ICC/IF

Immunofluorescent analysis of 4-Hydroxynonenal in human embryonic kidney cells (HEK293). Sample was fixed with 5% formaldehyde (5 min), incubated with 4-Hydroxynonenal monoclonal antibody (Product # MA5-27570) using a dilution of 1:50 (30-60 min at RT), and followed by Goat Anti-Mouse Alexa Fluor 488, Phalloidin Alexa Fluor 633 and DAPI secondary antibody at a dilution of 1:1500, 1:250 and 1:50,000 (30-60 m... View More

4-Hydroxynonenal Antibody in Western Blot (WB)

4-Hydroxynonenal Antibody in Immunohistochemistry (IHC)

4-Hydroxynonenal Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Product Details

MA5-27570

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50

- -

ELISA (ELISA)

1:1,000

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

12F7

Immunogen

Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735095

Product Specific Information

A 1:1,000 dilution of MA5-27570 was sufficient for detection of 4-Hydroxynonenal in 0.5 µg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.

Target Information

4-Hydroxynonenal, or 4-hydroxy-2-nonenal or 4-HNE or HNE, is an alpha, beta-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary alpha, beta-unsaturated hydroxyalkenal formed in this process.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets.

Nutrients

Li Q,Du Y,Xiang P,Chen G,Qian X,Li S,Mao Y,Ling W,Wang D

MA5-27570 was used in ELISA, Immunocytochemistry, Western Blot, Immunohistochemistry to investigate whether Brussels chicory can stabilize advanced plaques in the aorta via improving oxidative stress.

Mon Feb 06 00:00:00 EST 2023

Deltamethrin-Evoked ER Stress Promotes Neuroinflammation in the Adult Mouse Hippocampus.

Cells

Hossain MM,Toltin AC,Gamba LM,Molina MA

MA5-27570 was used in Western Blot to demonstrate that exposure to deltamethrin leads to ER stress-mediated neuroinflammation, which may subsequently contribute to neurodegeneration and cognitive impairment in mice.

Sat Jun 18 00:00:00 EDT 2022

Ranolazine Counteracts Strength Impairment and Oxidative Stress in Aged Sarcopenic Mice.

Metabolites

Torcinaro A,Cappetta D,De Santa F,Telesca M,Leigheb M,Berrino L,Urbanek K,De Angelis A,Ferraro E

MA5-27570 was used in Immunohistochemistry-immunofluorescence to investigate the ability of the metabolic modulator Ranolazine to counteract skeletal muscle dysfunctions that occur with aging.

Mon Jul 18 00:00:00 EDT 2022

Involvement of FSP1-CoQ₁₀-NADH and GSH-GPx-4 pathways in retinal pigment epithelium ferroptosis.

Cell death & disease

Yang M,Tsui MG,Tsang JKW,Goit RK,Yao KM,So KF,Lam WC,Lo ACY

MA5-27570 was used in Immunohistochemistry to describe a distinct role for ferroptosis in controlling RPE cell death in vitro and in vivo and may provide a new avenue for identifying treatment targets for RPE degeneration.

Wed May 18 00:00:00 EDT 2022

NOX4 inhibition promotes the remodeling of dystrophic muscle.

JCI insight

Hammers DW

MA5-27570 was used in ELISA, Immunocytochemistry, Western Blot, Immunohistochemistry to indicate that targeting NOX4 is an effective strategy to promote the beneficial remodeling of disease-burdened muscle representative of DMD and, potentially, other MDs and muscle pathologies.

Mon Oct 24 00:00:00 EDT 2022

Divergent erythroid megakaryocyte fates in Blvrb-deficient mice establish non-overlapping cytoprotective functions during stress hematopoiesis.

Free radical biology & medicine

Nesbitt NM,Malone LE,Liu Z,Jares A,Gnatenko DV,Ma Y,Zhu W,Bahou WF

MA5-27570 was used in Western Blot to provide the first physiological function of murine Blvrb in a non-redundant pathway of stress cytoprotection.

Sat Feb 20 00:00:00 EST 2021

Antioxidant activity of mesenchymal stem cell-derived extracellular vesicles restores hippocampal neurons following seizure damage.

Theranostics

Luo Q,Xian P,Wang T,Wu S,Sun T,Wang W,Wang B,Yang H,Yang Y,Wang H,Liu W,Long Q

Published figure using 4-Hydroxynonenal monoclonal antibody (Product # MA5-27570) in Western Blot

Fri Jul 30 00:00:00 EDT 2021

PLIN2 Mediates Neuroinflammation and Oxidative/Nitrosative Stress via Downregulating Phosphatidylethanolamine in the Rostral Ventrolateral Medulla of Stressed Hypertensive Rats.

Journal of inflammation research

Zhang S,Hu L,Han C,Huang R,Ooi K,Qian X,Ren X,Chu D,Zhang H,Du D,Xia C

Published figure using 4-Hydroxynonenal monoclonal antibody (Product # MA5-27570) in Immunohistochemistry

Fri Apr 29 00:00:00 EDT 2022

PGRMC1-dependent lipophagy promotes ferroptosis in paclitaxel-tolerant persister cancer cells.

Journal of experimental & clinical cancer research : CR

You JH,Lee J,Roh JL

MA5-27570 was used in Western Blot to study PGRMC1 in persister cancer cells.

Mon Nov 08 00:00:00 EST 2021

PGRMC1-dependent lipophagy promotes ferroptosis in paclitaxel-tolerant persister cancer cells.

Journal of experimental & clinical cancer research : CR

You JH,Lee J,Roh JL

MA5-27570 was used in Western Blot to study PGRMC1 in persister cancer cells.

Mon Nov 08 00:00:00 EST 2021

Hsp22 Deficiency Induces Age-Dependent Cardiac Dilation and Dysfunction by Impairing Autophagy, Metabolism, and Oxidative Response.

Antioxidants (Basel, Switzerland)

Wu W,Sun X,Shi X,Lai L,Wang C,Xie M,Qin G,Qiu H

MA5-27570 was used in Immunohistochemistry (Paraffin) to show that Hsp22 played an essential role in the non-stressed heart during the early stage of aging, which may bring new insight into understanding the pathogenesis of age-related dilated cardiomyopathy.

Wed Sep 29 00:00:00 EDT 2021

Hsp22 Deficiency Induces Age-Dependent Cardiac Dilation and Dysfunction by Impairing Autophagy, Metabolism, and Oxidative Response.

Antioxidants (Basel, Switzerland)

Wu W,Sun X,Shi X,Lai L,Wang C,Xie M,Qin G,Qiu H

Wed Sep 29 00:00:00 EDT 2021

4-Hydroxynonenal Contributes to Fibroblast Senescence in Skin Photoaging Evoked by UV-A Radiation.

Antioxidants (Basel, Switzerland)

Swiader A,Camaré C,Guerby P,Salvayre R,Negre-Salvayre A

MA5-27570 was used in Western Blot, Immunoprecipitation to emphasize the role of HNE and lipid peroxidation-derived aldehydes in fibroblast senescence, and confirm the protective effect of carnosine in skin photoaging.

Sun Feb 28 00:00:00 EST 2021

4-Hydroxynonenal Contributes to Fibroblast Senescence in Skin Photoaging Evoked by UV-A Radiation.

Antioxidants (Basel, Switzerland)

Swiader A,Camaré C,Guerby P,Salvayre R,Negre-Salvayre A

Sun Feb 28 00:00:00 EST 2021

Evidence of complement dysregulation in outer retina of Stargardt disease donor eyes.

Redox biology

Hu J,Pauer GJ,Hagstrom SA,Bok D,DeBenedictis MJ,Bonilha VL,Hollyfield JG,Radu RA

MA5-27570 was used in Immunohistochemistry (PFA fixed) to report the first direct evidence of retinal pigment epithelial complement dysregulation as a causative factor in developing Stargardt phenotype.

Thu Oct 01 00:00:00 EDT 2020

Evidence of complement dysregulation in outer retina of Stargardt disease donor eyes.

Redox biology

Hu J,Pauer GJ,Hagstrom SA,Bok D,DeBenedictis MJ,Bonilha VL,Hollyfield JG,Radu RA

Thu Oct 01 00:00:00 EDT 2020

Brain-Specific Serine-47 Modification of Cytochrome c Regulates Cytochrome c Oxidase Activity Attenuating ROS Production and Cell Death: Implications for Ischemia/Reperfusion Injury and Akt Signaling.

Cells

Kalpage HA,Wan J,Morse PT,Lee I,Hüttemann M

MA5-27570 was used in Western Blotting to show the mutation S47E in cytochrome c causes mitochondrial respiration dsyfunction.

Thu Aug 06 00:00:00 EDT 2020

Cells

Kalpage HA,Wan J,Morse PT,Lee I,Hüttemann M

MA5-27570 was used in Western Blotting to show the mutation S47E in cytochrome c causes mitochondrial respiration dsyfunction.

Thu Aug 06 00:00:00 EDT 2020

Epigenetic reprogramming of epithelial-mesenchymal transition promotes ferroptosis of head and neck cancer.

Redox biology

Lee J,You JH,Kim MS,Roh JL

MA5-27570 was used in Western Blotting to suggest that epigenetic reprogramming of EMT contributes to promoting ferroptosis in HNC cells.

Thu Oct 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: 4-HNE; 4-hydroxy Hexenal; 4-Hydroxy Nonenal; 4-hydroxy-2-nonenal; 4-Hydroxynonenal; 4HNE; HNE

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4-Hydroxynonenal Monoclonal Antibody (12F7)

4-Hydroxynonenal Antibody (MA5-27570) in ICC/IF

Product Details

MA5-27570

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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