Invitrogen

alpha Tubulin Monoclonal Antibody (DM1A), eFluor™ 615, eBioscience™

11 Published Figures

12 References

View all (82) alpha Tubulin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (11)

alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

alpha Tubulin Antibody (42-4502-82) in ICC/IF

Immunocytochemistry on fixed and permeabilized C6 cells using 1 µg/mL Anti-alpha Tubulin eFluor® 615. Nuclei are counterstained with DAPI.

alpha Tubulin Antibody in Immunocytochemistry, Proximity Ligation Assay (PLA) (ICC/IF, PLA)

alpha Tubulin Antibody in Western Blot (WB)

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Product Details

42-4502-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1 µg/mL

View 5 publications 5 publications

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Pig, Rat

Published species

Non-human primate

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 615, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DM1A

Conjugate

eFluor™ 615 eFluor™ 615 eFluor™ 615

Excitation/Emission Max

595/614 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10853970

Product Specific Information

Description: The monoclonal antibody DM1a recognizes the 50kDa cytoskeletal protein alpha tubulin in a variety of species (human, mouse, rat, monkey, dog, pig, bovine, goat, hamster, guinea pig, kangaroo, amphibians, sea urchin, yeast and tobacco plants). Tubulin, the major component of microtubules, is a dimeric protein consisting of an alpha and beta subunit. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation resulting in assembly (polymerization) or disassembly (depolymerization). The dynamic nature of microtubules is most evident in the mitotic apparatus. The DM1a antibody recognizes the C-terminal end of the alpha tubulin isoform (amino acids 426-430).

Applications Reported: This DM1A antibody has been reported for use in immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded (FFPE, IHC-P) tissue sections as well as immunocytochemical staining.

Applications Tested: This DM1A antibody has been tested by immunocytochemistry on fixed and permeabilized C6 cells at less than or equal to 1 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.

Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.

Excitation: 595 nm; Emission: 615 nm.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.

The Journal of cell biology

Zhang Y,Seemann J

Published figure using alpha Tubulin monoclonal antibody (Product # 42-4502-82) in Immunocytochemistry

Mon Jan 04 00:00:00 EST 2021

Targeting EML4-ALK gene fusion variant 3 in thyroid cancer.

Endocrine-related cancer

Aydemirli MD,van Eendenburg JDH,van Wezel T,Oosting J,Corver WE,Kapiteijn E,Morreau H

Published figure using alpha Tubulin monoclonal antibody (Product # 42-4502-82) in Western Blot

Tue May 11 00:00:00 EDT 2021

Giant ankyrin-B suppresses stochastic collateral axon branching through direct interaction with microtubules.

The Journal of cell biology

Chen K,Yang R,Li Y,Zhou JC,Zhang M

Published figure using alpha Tubulin monoclonal antibody (Product # 42-4502-82) in Immunocytochemistry

Mon Aug 03 00:00:00 EDT 2020

Loss of the Nuclear Protein RTF2 Enhances Influenza Virus Replication.

Journal of virology

Chia BS,Li B,Cui A,Eisenhaure T,Raychowdhury R,Lieb D,Hacohen N

Published figure using alpha Tubulin monoclonal antibody (Product # 42-4502-82) in Western Blot

Tue Oct 27 00:00:00 EDT 2020

PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells.

Molecular neurobiology

Winkler SC,Shimobayashi E,Kapfhammer JP

Published figure using alpha Tubulin monoclonal antibody (Product # 42-4502-82) in Immunocytochemistry

Tue Dec 01 00:00:00 EST 2020

PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells.

Molecular neurobiology

Winkler SC,Shimobayashi E,Kapfhammer JP

Published figure using alpha Tubulin monoclonal antibody (Product # 42-4502-82) in Immunocytochemistry

Tue Dec 01 00:00:00 EST 2020

Metabolic reprogramming related to whole-chromosome instability in models for Hürthle cell carcinoma.

Scientific reports

Addie RD,Kostidis S,Corver WE,Oosting J,Aminzadeh-Gohari S,Feichtinger RG,Kofler B,Aydemirli MD,Giera M,Morreau H

Published figure using alpha Tubulin monoclonal antibody (Product # 42-4502-82) in Western Blot

Fri Jun 12 00:00:00 EDT 2020

GATA factor-regulated solute carrier ensemble reveals a nucleoside transporter-dependent differentiation mechanism.

PLoS genetics

Zwifelhofer NM,Cai X,Liao R,Mao B,Conn DJ,Mehta C,Keles S,Xia Y,Bresnick EH

Published figure using alpha Tubulin monoclonal antibody (Product # 42-4502-82) in Western Blot

Tue Dec 01 00:00:00 EST 2020

RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.

Proceedings of the National Academy of Sciences of the United States of America

Gulbranson DR,Davis EM,Demmitt BA,Ouyang Y,Ye Y,Yu H,Shen J

Published figure using alpha Tubulin monoclonal antibody (Product # 42-4502-82) in Western Blot

Tue Sep 26 00:00:00 EDT 2017

Do mechanical strain and TNF-α interact to amplify pro-inflammatory cytokine production in human annulus fibrosus cells?

Journal of biomechanics

Likhitpanichkul M,Torre OM,Gruen J,Walter BA,Hecht AC,Iatridis JC

Published figure using alpha Tubulin monoclonal antibody (Product # 42-4502-82) in Immunocytochemistry

Tue May 03 00:00:00 EDT 2016

IP-10/CXCL10 induction in human pancreatic cancer stroma influences lymphocytes recruitment and correlates with poor survival.

Oncotarget

Lunardi S,Jamieson NB,Lim SY,Griffiths KL,Carvalho-Gaspar M,Al-Assar O,Yameen S,Carter RC,McKay CJ,Spoletini G,D'Ugo S,Silva MA,Sansom OJ,Janssen KP,Muschel RJ,Brunner TB

Published figure using alpha Tubulin monoclonal antibody (Product # 42-4502-82) in Western Blot

Sun Nov 30 00:00:00 EST 2014

Chikungunya virus nsP3 blocks stress granule assembly by recruitment of G3BP into cytoplasmic foci.

Journal of virology

Fros JJ,Domeradzka NE,Baggen J,Geertsema C,Flipse J,Vlak JM,Pijlman GP

Published figure using alpha Tubulin monoclonal antibody (Product # 42-4502-82) in Immunofluorescence

Mon Oct 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: alpha tubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; beta-tubulin T beta15 (aa 1-445); class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; T beta-15; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; tubulin alpha 3; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2; tubulin, beta 2B class IIb; tubulin, beta polypeptide paralog

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Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; PMGYSA; RGD1309427; RGD1565476; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; Tubb2; Tubb2a; TUBB2B

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Function(s)

GTPase activity structural molecule activity structural constituent of cytoskeleton protein binding GTP binding protein domain specific binding double-stranded RNA binding ubiquitin protein ligase binding molecular_function enzyme binding nucleotide binding protein heterodimerization activity tubulin

Process(es)

G2/M transition of mitotic cell cycle cytoskeleton organization microtubule-based process cytoskeleton-dependent intracellular transport cell division cellular response to interleukin-4 microtubule cytoskeleton organization biological_process platelet degranulation neuron migration protein polymerization metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Tubulin Monoclonal Antibody (DM1A), eFluor™ 615, eBioscience™

alpha Tubulin Antibody (42-4502-82) in ICC/IF

Product Details

42-4502-82

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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