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Invitrogen
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 615, eBioscience™
FIGURE: 1 / 5
Mouse IgG1 kappa Isotype Control (42-4714-80) in IHC (P)
Immunohistochemistry on formalin-fixed paraffin embedded human breast carcinoma using 20 µg/mL of Mouse IgG1 Isotype Control eFluor® 615 (left) or 20 µg/mL Anti-Human CD277 (Mucin 1) eFluor® 615 (right). Nuclei are counterstained with DAPI.
Product Details
42-4714-80
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
20 µg/mL
Immunocytochemistry (ICC/IF)
1-10 µg/mL
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Isotype Control
Clone
P3.6.2.8.1
Conjugate
Excitation/Emission Max
595/614 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10733160
Product Specific Information
Description: The monoclonal mouse IgG1 kappa immunoglobulin is useful as an isotype control.
Applications Reported: Mouse IgG1 K Isotype Control eFluor® 615 has been reported for use in immunocytochemical (ICC) and immunohistochemical (IHC-P) staining.
Applications Tested: This Mouse IgG1, K Isotype Control eFluor® 615 has been tested by immunocytochemistry on fixed and permeabilized MDCK cells and by immunohistochemistry (IHC-P) on formalin-fixed paraffin embedded human brain tissue. Use isotype control at the same concentration as experimental antibody.
Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength. A standard Alexa Fluor® 594 filter is acceptable.
Excitation: 595 nm; Emission: 615 nm.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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