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Invitrogen
Vimentin Monoclonal Antibody (V9), eFluor™ 615, eBioscience™
Product Details
42-9897-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Human,
Rat
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
V9
Conjugate
Excitation/Emission Max
595/614 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10870779
Product Specific Information
Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, as well as pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils.
This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin.
Applications Reported: This V9 antibody has been reported for use in immunocytochemistry and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P).
Applications Tested: This V9 antibody has been tested by immunocytochemistry on fixed and permeabilized C6 cells at less than or equal to 1 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.
Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.
Excitation: 595 nm; Emission: 615 nm.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal protein 113; FLJ36605; RP11-124N14.1; VIME; Vimentin; vimentin C-terminus
Gene Aliases: CTRCT30; HEL113; VIM
UniProt ID: (Human) P08670, (Dog) A0A8C0N8E3, (Rat) P31000
Entrez Gene ID: (Chicken) 420519, (Human) 7431, (Dog) 477991, (Rat) 81818
glycoprotein binding
double-stranded RNA binding
structural constituent of cytoskeleton
structural constituent of eye lens
protein binding
protein C-terminus binding
identical protein binding
scaffold protein binding
keratin filament binding
structural molecule activity
kinase binding
protein kinase binding
protein phosphatase 2A binding
movement of cell or subcellular component
positive regulation of gene expression
negative regulation of neuron projection development
astrocyte development
viral process
muscle filament sliding
intermediate filament organization
Bergmann glial cell differentiation
SMAD protein signal transduction
lens fiber cell development
aging
response to radiation
response to zinc ion
response to selenium ion
response to estradiol
response to vitamin D
skeletal muscle tissue regeneration
cellular response to fibroblast growth factor stimulus
intermediate filament-based process
fat cell differentiation
decidualization
regulation of axonogenesis
positive regulation of glial cell proliferation
regulation of Schwann cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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