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Invitrogen
CD117 (c-Kit) Monoclonal Antibody (ACK2), PerCP-eFluor™ 710, eBioscience™
FIGURE: 1 / 23
CD117 (c-Kit) Antibody (46-1172-82) in Flow
C57BL/6 mouse bone marrow cells were stained with Mouse Hematopoietic Lineage Antibody Cocktail, eFluor 450 (Product # 88-7772-72), Ly-6A/E (Sca-1) Monoclonal Antibody, PE (Product # 12-5981-82) and 0.125 µg of Rat IgG2b kappa Isotype Control, PerCP-eFluor 710 (Product # 46-4031-82) (left) or 0.125 µg of CD117 (c-Kit) Monoclonal Antibody, PerCP-eFluor 710 (right). Cells in the lineage low/negative gate were used for analysis.
Product Details
46-1172-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
ACK2
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815144
Product Specific Information
Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.
Applications Reported: This ACK2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: belly-spot; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit
Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; Kit; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W
UniProt ID: (Mouse) P05532
Entrez Gene ID: (Mouse) 16590
nucleotide binding
protease binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
stem cell factor receptor activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
cytokine binding
protein homodimerization activity
metal ion binding
transmembrane signal receptor
activation of MAPK activity
ovarian follicle development
myeloid progenitor cell differentiation
lymphoid progenitor cell differentiation
immature B cell differentiation
dendritic cell cytokine production
mast cell chemotaxis
myeloid leukocyte differentiation
protein phosphorylation
glycosphingolipid metabolic process
chemotaxis
inflammatory response
transmembrane receptor protein tyrosine kinase signaling pathway
germ cell development
spermatogenesis
spermatid development
positive regulation of cell proliferation
germ cell migration
regulation of cell shape
visual learning
response to radiation
positive regulation of gene expression
phosphorylation
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
lamellipodium assembly
hemopoiesis
cell differentiation
T cell differentiation
erythrocyte differentiation
melanocyte differentiation
positive regulation of cell migration
positive regulation of pseudopodium assembly
actin cytoskeleton reorganization
mast cell cytokine production
somatic stem cell population maintenance
embryonic hemopoiesis
ectopic germ cell programmed cell death
intracellular signal transduction
megakaryocyte development
Fc receptor signaling pathway
Kit signaling pathway
erythropoietin-mediated signaling pathway
positive regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of programmed cell death
mast cell degranulation
positive regulation of MAP kinase activity
positive regulation of MAPK cascade
pigmentation
positive regulation of Notch signaling pathway
positive regulation of JAK-STAT cascade
protein autophosphorylation
developmental pigmentation
regulation of developmental pigmentation
somatic stem cell division
positive regulation of long-term neuronal synaptic plasticity
digestive tract development
stem cell differentiation
epithelial cell proliferation
detection of mechanical stimulus involved in sensory perception of sound
positive regulation of sequence-specific DNA binding transcription factor activity
cell chemotaxis
mast cell differentiation
cellular response to thyroid hormone stimulus
melanocyte migration
melanocyte adhesion
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