Invitrogen

CD184 (CXCR4) Monoclonal Antibody (2B11), PerCP-eFluor™ 710, eBioscience™

CD184 (CXCR4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 14

CD184 (CXCR4) Antibody (46-9991-82) in Flow

Staining of C57Bl/6 thymocytes with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82) and 0.5 µg of Rat IgG2b kappa Isotype Control PerCP-eFluor® 710 (Product # 46-4031-82) (left) or 0.5 µg of Anti-Mouse CD184 (CXCR4) PerCP-eFluor® 710 (right). Total viable cells were used for analysis.

Product Image Gallery

Product Details

46-9991-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2B11

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10670489

Product Specific Information

Description: The 2B11 monoclonal antibody reacts with mouse and human CXCR4, also known as CD184, Fusin, LESTR, or HUMSTR. Fusin is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. In addition it has been shown to play a role in lymphocyte migration. The ligand for CXCR4 is SDF1. CXCR4 is required for maintenance of granulocyte progenitors in the bone marrow and essential for B cell development. Expression of CXCR4 is different between mice and human. In the thymus CXCR4 is restricted to CD4+CD8+ cells while in the spleen predominant expression is found on B lymphocytes.

The monoclonal antibody 2B11 recognizes the amino terminus of CXCR4 and has been reported to have functional activity; it does not block SDF1a but causes internalization of the receptor.

Applications Reported: This 2B11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2B11 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Designing Synthetic, Sulfated Glycosaminoglycan Mimetics That Are Orally Bioavailable and Exhibiting In Vivo Anticancer Activity.

Journal of medicinal chemistry

Morla S,Ravikumar O,O'Hara C,Boothello R,Vera A,Abdelfadiel EI,Fayyad R,Afosah DK,Sharon C,Fernandez L,Shah SA,Patel BB,Desai UR

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 46-9991-82) in Flow Cytometry

Thu Jan 26 00:00:00 EST 2023

PHGDH is required for germinal center formation and is a therapeutic target in MYC-driven lymphoma.

The Journal of clinical investigation

D'Avola A,Legrave N,Tajan M,Chakravarty P,Shearer RL,King HW,Kluckova K,Cheung EC,Clear AJ,Gunawan AS,Zhang L,James LK,MacRae JI,Gribben JG,Calado DP,Vousden KH,Riches JC

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 46-9991-82) in Flow Cytometry

Mon May 02 00:00:00 EDT 2022

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 46-9991-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Plasmacytoid Dendritic Cells and Type I Interferon Promote Extrafollicular B Cell Responses to Extracellular Self-DNA.

Immunity

Soni C,Perez OA,Voss WN,Pucella JN,Serpas L,Mehl J,Ching KL,Goike J,Georgiou G,Ippolito GC,Sisirak V,Reizis B

46-9991-82 was used in Flow Cytometry to establish extrafollicular B cell differentiation into short-lived AFCs as a key mechanism of anti-DNA autoreactivity and reveal a major contribution of pDCs, endosomal Toll-like receptors (TLRs), and IFN-I to this pathway.

Tue Jun 16 00:00:00 EDT 2020

A Hyper-IgM Syndrome Mutation in Activation-Induced Cytidine Deaminase Disrupts G-Quadruplex Binding and Genome-wide Chromatin Localization.

Immunity

Yewdell WT,Kim Y,Chowdhury P,Lau CM,Smolkin RM,Belcheva KT,Fernandez KC,Cols M,Yen WF,Vaidyanathan B,Angeletti D,McDermott AB,Yewdell JW,Sun JC,Chaudhuri J

46-9991-82 was used in Flow cytometry/Cell sorting to indicate a crucial role for G4 binding in AID targeting and suggest that AID activity may extend beyond Ig loci to regulate the expression of genes relevant to the physiology and pathology of activated B cells.

Tue Nov 17 00:00:00 EST 2020

SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation.

Immunity

Lederer K,Castaño D,Gómez Atria D,Oguin TH,Wang S,Manzoni TB,Muramatsu H,Hogan MJ,Amanat F,Cherubin P,Lundgreen KA,Tam YK,Fan SHY,Eisenlohr LC,Maillard I,Weissman D,Bates P,Krammer F,Sempowski GD,Pardi N,Locci M

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 46-9991-82) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2020

Inhibition of Migration, Invasion and Drug Resistance of Pancreatic Adenocarcinoma Cells - Role of Snail, Slug and Twist and Small Molecule Inhibitors.

OncoTargets and therapy

Kaşıkcı E,Aydemir E,Bayrak ÖF,Şahin F

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 46-9991-82) in Flow Cytometry

Fri May 20 00:00:00 EDT 2022

Genetic timestamping of plasma cells in vivo reveals tissue-specific homeostatic population turnover.

eLife

Xu AQ,Barbosa RR,Calado DP

46-9991 was used in Flow cytometry/Cell sorting to characterize a genetic tool (JchaincreERT2) in the mouse that permits first-ever specific genetic manipulation in PCs in vivo, across immunoglobulin isotypes.

Mon Nov 02 00:00:00 EST 2020

Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules.

NPJ vaccines

Andersen TK,Huszthy PC,Gopalakrishnan RP,Jacobsen JT,Fauskanger M,Tveita AA,Grødeland G,Bogen B

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 46-9991-82) in Flow Cytometry

Thu Oct 05 00:00:00 EDT 2023

Precardiac organoids form two heart fields via Bmp/Wnt signaling.

Nature communications

Andersen P,Tampakakis E,Jimenez DV,Kannan S,Miyamoto M,Shin HK,Saberi A,Murphy S,Sulistio E,Chelko SP,Kwon C

46-9991 was used in Flow cytometry/Cell sorting to study the specification of two cardiac origins via Bmp/Wnt signalling.

Tue Aug 07 00:00:00 EDT 2018

A Functional Bioluminescent Zebrafish Screen for Enhancing Hematopoietic Cell Homing.

Stem cell reports

Astuti Y,Kramer AC,Blake AL,Blazar BR,Tolar J,Taisto ME,Lund TC

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 46-9991-82) in Flow Cytometry

Tue Jan 10 00:00:00 EST 2017

Loss of MAPK-activated protein kinase 2 enables potent dendritic cell-driven anti-tumour T cell response.

Scientific reports

Soukup K,Halfmann A,Dillinger B,Poyer F,Martin K,Blauensteiner B,Kauer M,Kuttke M,Schabbauer G,Dohnal AM

46-9991 was used in Flow cytometry/Cell sorting to suggest that MAPK-activated protein kinase 2 deletion leads to an expansion of stimulatory CD103+ dendritic cells, mounting a potent CD8+ T cell anti-tumour response in mice.

Mon Sep 18 00:00:00 EDT 2017

Induced Pluripotent Stem Cells derived Muscle Progenitors Effectively Mitigate Muscular Dystrophy through Restoring the Dystrophin Distribution.

Journal of stem cell research & therapy

Cai WF,Huang W,Wang L,Wang JP,Zhang L,Ashraf M,Wu S,Wang Y

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 46-9991-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Inhibition of insulin-like growth factor receptor/AKT/mammalian target of rapamycin axis targets colorectal cancer stem cells by attenuating mevalonate-isoprenoid pathway in vitro and in vivo.

Oncotarget

Sharon C,Baranwal S,Patel NJ,Rodriguez-Agudo D,Pandak WM,Majumdar AP,Krystal G,Patel BB

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 46-9991-82) in Flow Cytometry

Sat Jun 20 00:00:00 EDT 2015

Intravenous transplantation of very small embryonic like stem cells in treatment of diabetes mellitus.

Avicenna journal of medical biotechnology

Abouzaripour M,Ragerdi Kashani I,Pasbakhsh P,Atlasy N

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 46-9991-82) in Flow Cytometry

Thu Apr 30 00:00:00 EDT 2015

Fibrocytes in the fibrotic lung: altered phenotype detected by flow cytometry.

Frontiers in pharmacology

Reese C,Lee R,Bonner M,Perry B,Heywood J,Silver RM,Tourkina E,Visconti RP,Hoffman S

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 46-9991-82) in Flow Cytometry

Mon Jul 07 00:00:00 EDT 2014

A new mouse model for female genital schistosomiasis.

PLoS neglected tropical diseases

Richardson ML,Fu CL,Pennington LF,Honeycutt JD,Odegaard JI,Hsieh YJ,Hammam O,Conti SL,Hsieh MH

46-9991 was used in Flow cytometry/Cell sorting to establish a new mouse model that could potentially enable novel studies of genital schistosomiasis in females.

Thu May 01 00:00:00 EDT 2014

Intramuscular VEGF activates an SDF1-dependent progenitor cell cascade and an SDF1-independent muscle paracrine cascade for cardiac repair.

American journal of physiology. Heart and circulatory physiology

Zisa D,Shabbir A,Mastri M,Taylor T,Aleksic I,McDaniel M,Suzuki G,Lee T

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 46-9991-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Intramuscular VEGF repairs the failing heart: role of host-derived growth factors and mobilization of progenitor cells.

American journal of physiology. Regulatory, integrative and comparative physiology

Zisa D,Shabbir A,Mastri M,Suzuki G,Lee T

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 46-9991-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; chemokine (C-X-C) receptor 4; chemokine receptor 4; CXC; CXC motif chemokine; CXC-R4; CXCR-4; Fusin; HUMSTR; LESTR; Leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; PB-CKR; Pre-B-cell-derived chemokine receptor; SDF-1 receptor; Stromal cell-derived factor 1 receptor

View more View less

Gene Aliases: b2b220Clo; CD184; Cmkar4; Cxcr4; LESTR; PB-CKR; PBSF/SDF-1; Sdf1r

View more View less

UniProt ID: (Mouse) P70658

View more View less

Entrez Gene ID: (Mouse) 12767

View more View less

Function(s)

actin binding signal transducer activity G-protein coupled receptor activity chemokine receptor activity protein binding drug binding C-X-C chemokine receptor activity cytokine binding ubiquitin protein ligase binding myosin light chain binding ubiquitin binding

Process(es)

patterning of blood vessels ameboidal-type cell migration neuron migration epithelial cell development chemotaxis signal transduction G-protein coupled receptor signaling pathway germ cell development nervous system development brain development motor neuron axon guidance germ cell migration organ morphogenesis cell migration calcium-mediated signaling telencephalon cell migration regulation of cell migration T cell proliferation neutrophil activation regulation of programmed cell death myelin maintenance positive regulation of oligodendrocyte differentiation regulation of chemotaxis regulation of calcium ion transport cardiac muscle contraction chemokine-mediated signaling pathway cellular response to cytokine stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD184 (CXCR4) Monoclonal Antibody (2B11), PerCP-eFluor™ 710, eBioscience™

CD184 (CXCR4) Antibody (46-9991-82) in Flow

Product Details

46-9991-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!