Invitrogen

CD184 (CXCR4) Monoclonal Antibody (2B11), Alexa Fluor™ 488, eBioscience™

CD184 (CXCR4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 17

CD184 (CXCR4) Antibody (53-9991-80) in Flow

Staining of C57Bl/6 thymocytes with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82) and 0.5 µg of Rat IgG2b kappa Isotype Control Alexa Fluor® 488 (Product # 53-4031-80) (left) or 0.5 µg of Anti-Mouse CD184 (CXCR4) Alexa Fluor® 488 (right). Total viable cells were used for analysis.

CD184 (CXCR4) Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

53-9991-80

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

1 µg/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2B11

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_953573

Product Specific Information

Description: The 2B11 monoclonal antibody reacts with mouse and human CXCR4, also known as CD184, Fusin, LESTR, or HUMSTR. Fusin is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. In addition it has been shown to play a role in lymphocyte migration. The ligand for CXCR4 is SDF1. CXCR4 is required for maintenance of granulocyte progenitors in the bone marrow and essential for B cell development. Expression of CXCR4 is different between mice and human. In the thymus CXCR4 is restricted to CD4+CD8+ cells while in the spleen predominant expression is found on B lymphocytes.

The monoclonal antibody 2B11 recognizes the amino terminus of CXCR4 and has been reported to have functional activity; it does not block SDF1a but causes internalization of the receptor.

Applications Reported: This 2B11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2B11 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Designing Synthetic, Sulfated Glycosaminoglycan Mimetics That Are Orally Bioavailable and Exhibiting In Vivo Anticancer Activity.

Journal of medicinal chemistry

Morla S,Ravikumar O,O'Hara C,Boothello R,Vera A,Abdelfadiel EI,Fayyad R,Afosah DK,Sharon C,Fernandez L,Shah SA,Patel BB,Desai UR

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Flow Cytometry

Thu Jan 26 00:00:00 EST 2023

PHGDH is required for germinal center formation and is a therapeutic target in MYC-driven lymphoma.

The Journal of clinical investigation

D'Avola A,Legrave N,Tajan M,Chakravarty P,Shearer RL,King HW,Kluckova K,Cheung EC,Clear AJ,Gunawan AS,Zhang L,James LK,MacRae JI,Gribben JG,Calado DP,Vousden KH,Riches JC

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Flow Cytometry

Mon May 02 00:00:00 EDT 2022

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Abrignani S,Addo R,Akdis M,Andrä I,Andreata F,Annunziato F,Arranz E,Bacher P,Bari S,Barnaba V,Barros-Martins J,Baumjohann D,Beccaria CG,Bernardo D,Boardman DA,Borger J,Böttcher C,Brockmann L,Burns M,Busch DH,Cameron G,Cammarata I,Cassotta A,Chang Y,Chirdo FG,Christakou E,Čičin-Šain L,Cook L,Corbett AJ,Cornelis R,Cosmi L,Davey MS,De Biasi S,De Simone G,Del Zotto G,Delacher M,Di Rosa F,Di Santo J,Diefenbach A,Dong J,Dörner T,Dress RJ,Dutertre CA,Eckle SBG,Eede P,Evrard M,Falk CS,Feuerer M,Fillatreau S,Fiz-Lopez A,Follo M,Foulds GA,Fröbel J,Gagliani N,Galletti G,Gangaev A,Garbi N,Garrote JA,Geginat J,Gherardin NA,Gibellini L,Ginhoux F,Godfrey DI,Gruarin P,Haftmann C,Hansmann L,Harpur CM,Hayday AC,Heine G,Hernández DC,Herrmann M,Hoelsken O,Huang Q,Huber S,Huber JE,Huehn J,Hundemer M,Hwang WYK,Iannacone M,Ivison SM,Jäck HM,Jani PK,Keller B,Kessler N,Ketelaars S,Knop L,Knopf J,Koay HF,Kobow K,Kriegsmann K,Kristyanto H,Krueger A,Kuehne JF,Kunze-Schumacher H,Kvistborg P,Kwok I,Latorre D,Lenz D,Levings MK,Lino AC,Liotta F,Long HM,Lugli E,MacDonald KN,Maggi L,Maini MK,Mair F,Manta C,Manz RA,Mashreghi MF,Mazzoni A,McCluskey J,Mei HE,Melchers F,Melzer S,Mielenz D,Monin L,Moretta L,Multhoff G,Muñoz LE,Muñoz-Ruiz M,Muscate F,Natalini A,Neumann K,Ng LG,Niedobitek A,Niemz J,Almeida LN,Notarbartolo S,Ostendorf L,Pallett LJ,Patel AA,Percin GI,Peruzzi G,Pinti M,Pockley AG,Pracht K,Prinz I,Pujol-Autonell I,Pulvirenti N,Quatrini L,Quinn KM,Radbruch H,Rhys H,Rodrigo MB,Romagnani C,Saggau C,Sakaguchi S,Sallusto F,Sanderink L,Sandrock I,Schauer C,Scheffold A,Scherer HU,Schiemann M,Schildberg FA,Schober K,Schoen J,Schuh W,Schüler T,Schulz AR,Schulz S,Schulze J,Simonetti S,Singh J,Sitnik KM,Stark R,Starossom S,Stehle C,Szelinski F,Tan L,Tarnok A,Tornack J,Tree TIM,van Beek JJP,van de Veen W,van Gisbergen K,Vasco C,Verheyden NA,von Borstel A,Ward-Hartstonge KA,Warnatz K,Waskow C,Wiedemann A,Wilharm A,Wing J,Wirz O,Wittner J,Yang JHM,Yang J

53-9991-80 was used in Flow cytometry/Cell sorting to provide the key aspects to consider when performing flow cytometry experiments.

Wed Dec 01 00:00:00 EST 2021

Orthogonal control of mean and variability of endogenous genes in a human cell line.

Nature communications

Bonny AR,Fonseca JP,Park JE,El-Samad H

53-9991-80 was used in Flow Cytometry to conclude that TuNR is therefore a modular tool that can be used in mammalian cells to enable direct interrogation of the implications of cell-to-cell variability.

Tue Jan 12 00:00:00 EST 2021

SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation.

Immunity

Lederer K,Castaño D,Gómez Atria D,Oguin TH,Wang S,Manzoni TB,Muramatsu H,Hogan MJ,Amanat F,Cherubin P,Lundgreen KA,Tam YK,Fan SHY,Eisenlohr LC,Maillard I,Weissman D,Bates P,Krammer F,Sempowski GD,Pardi N,Locci M

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2020

Inhibition of Migration, Invasion and Drug Resistance of Pancreatic Adenocarcinoma Cells - Role of Snail, Slug and Twist and Small Molecule Inhibitors.

OncoTargets and therapy

Kaşıkcı E,Aydemir E,Bayrak ÖF,Şahin F

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Flow Cytometry

Fri May 20 00:00:00 EDT 2022

Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules.

NPJ vaccines

Andersen TK,Huszthy PC,Gopalakrishnan RP,Jacobsen JT,Fauskanger M,Tveita AA,Grødeland G,Bogen B

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Flow Cytometry

Thu Oct 05 00:00:00 EDT 2023

The Chemokine Receptor CXCR4 Mediates Recruitment of CD11c+ Conventional Dendritic Cells Into the Inflamed Murine Cornea.

Investigative ophthalmology & visual science

Lopez MJ,Seyed-Razavi Y,Jamali A,Harris DL,Hamrah P

53-9991 was used in Flow cytometry/Cell sorting to investigate the presence of CXCR4 and its ligand, CXCL12, in the murine cornea, and its role in conventional dendritic cell migration during corneal inflammation.

Thu Nov 01 00:00:00 EDT 2018

Precardiac organoids form two heart fields via Bmp/Wnt signaling.

Nature communications

Andersen P,Tampakakis E,Jimenez DV,Kannan S,Miyamoto M,Shin HK,Saberi A,Murphy S,Sulistio E,Chelko SP,Kwon C

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Immunofluorescence

Tue Aug 07 00:00:00 EDT 2018

A Functional Bioluminescent Zebrafish Screen for Enhancing Hematopoietic Cell Homing.

Stem cell reports

Astuti Y,Kramer AC,Blake AL,Blazar BR,Tolar J,Taisto ME,Lund TC

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Flow Cytometry

Tue Jan 10 00:00:00 EST 2017

Induced Pluripotent Stem Cells derived Muscle Progenitors Effectively Mitigate Muscular Dystrophy through Restoring the Dystrophin Distribution.

Journal of stem cell research & therapy

Cai WF,Huang W,Wang L,Wang JP,Zhang L,Ashraf M,Wu S,Wang Y

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Optochemokine Tandem for Light-Control of Intracellular Ca2.

PloS one

Feldbauer K,Schlegel J,Weissbecker J,Sauer F,Wood PG,Bamberg E,Terpitz U

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Immunofluorescence

Tue Jun 20 00:00:00 EDT 2017

Inhibition of insulin-like growth factor receptor/AKT/mammalian target of rapamycin axis targets colorectal cancer stem cells by attenuating mevalonate-isoprenoid pathway in vitro and in vivo.

Oncotarget

Sharon C,Baranwal S,Patel NJ,Rodriguez-Agudo D,Pandak WM,Majumdar AP,Krystal G,Patel BB

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Flow Cytometry

Sat Jun 20 00:00:00 EDT 2015

Intravenous transplantation of very small embryonic like stem cells in treatment of diabetes mellitus.

Avicenna journal of medical biotechnology

Abouzaripour M,Ragerdi Kashani I,Pasbakhsh P,Atlasy N

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Flow Cytometry

Thu Apr 30 00:00:00 EDT 2015

Fibrocytes in the fibrotic lung: altered phenotype detected by flow cytometry.

Frontiers in pharmacology

Reese C,Lee R,Bonner M,Perry B,Heywood J,Silver RM,Tourkina E,Visconti RP,Hoffman S

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Flow Cytometry

Mon Jul 07 00:00:00 EDT 2014

Tumor necrosis factor induces tumor promoting and anti-tumoral effects on pancreatic cancer via TNFR1.

PloS one

Chopra M,Lang I,Salzmann S,Pachel C,Kraus S,Bäuerlein CA,Brede C,Garrote AL,Mattenheimer K,Ritz M,Schwinn S,Graf C,Schäfer V,Frantz S,Einsele H,Wajant H,Beilhack A

53-9991 was used in Flow cytometry/Cell sorting to assess the mechanisms underlying the contribution of tumour necrosis factor to carcinogenesis.

Mon May 12 00:00:00 EDT 2014

Intramuscular VEGF activates an SDF1-dependent progenitor cell cascade and an SDF1-independent muscle paracrine cascade for cardiac repair.

American journal of physiology. Heart and circulatory physiology

Zisa D,Shabbir A,Mastri M,Taylor T,Aleksic I,McDaniel M,Suzuki G,Lee T

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Immunofluorescence

Thu Dec 01 00:00:00 EST 2011

Intramuscular VEGF activates an SDF1-dependent progenitor cell cascade and an SDF1-independent muscle paracrine cascade for cardiac repair.

American journal of physiology. Heart and circulatory physiology

Zisa D,Shabbir A,Mastri M,Taylor T,Aleksic I,McDaniel M,Suzuki G,Lee T

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Immunofluorescence

Thu Dec 01 00:00:00 EST 2011

Intramuscular VEGF repairs the failing heart: role of host-derived growth factors and mobilization of progenitor cells.

American journal of physiology. Regulatory, integrative and comparative physiology

Zisa D,Shabbir A,Mastri M,Suzuki G,Lee T

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 53-9991-80) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; chemokine (C-X-C) receptor 4; chemokine receptor 4; CXC; CXC motif chemokine; CXC-R4; CXCR-4; Fusin; HUMSTR; LESTR; Leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; PB-CKR; Pre-B-cell-derived chemokine receptor; SDF-1 receptor; Stromal cell-derived factor 1 receptor

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Gene Aliases: b2b220Clo; CD184; Cmkar4; Cxcr4; LESTR; PB-CKR; PBSF/SDF-1; Sdf1r

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UniProt ID: (Mouse) P70658

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Entrez Gene ID: (Mouse) 12767

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Function(s)

actin binding signal transducer activity G-protein coupled receptor activity chemokine receptor activity protein binding drug binding C-X-C chemokine receptor activity cytokine binding ubiquitin protein ligase binding myosin light chain binding ubiquitin binding

Process(es)

patterning of blood vessels ameboidal-type cell migration neuron migration epithelial cell development chemotaxis signal transduction G-protein coupled receptor signaling pathway germ cell development nervous system development brain development motor neuron axon guidance germ cell migration organ morphogenesis cell migration calcium-mediated signaling telencephalon cell migration regulation of cell migration T cell proliferation neutrophil activation regulation of programmed cell death myelin maintenance positive regulation of oligodendrocyte differentiation regulation of chemotaxis regulation of calcium ion transport cardiac muscle contraction chemokine-mediated signaling pathway cellular response to cytokine stimulus

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CD184 (CXCR4) Monoclonal Antibody (2B11), Alexa Fluor™ 488, eBioscience™

CD184 (CXCR4) Antibody (53-9991-80) in Flow

Product Details

53-9991-80

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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