Invitrogen

CD326 (EpCAM) Monoclonal Antibody (G8.8), APC-eFluor™ 780, eBioscience™

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Antibody Testing Data (1)
Published Figures (69)

CD326 (EpCAM) Antibody in Flow Cytometry (Flow)

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CD326 (EpCAM) Antibody (47-5791-82) in Flow

Staining of the TE-71 cell line with 0.06 µg of Rat IgG2a K Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (blue histogram) or 0.06 µg of Anti-Mouse CD326 (EpCAM) APC-eFluor® 780 (purple histogram). Total viable cells were used for analysis.

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Product Details

47-5791-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 59 publications 59 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

G8.8

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573986

Product Specific Information

Description: The G8.8 monoclonal antibody reacts with the 40 kDa protein mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40 (epithelial glycoprotein 40), 17-1A antigen, TACSTD1 (tumor-associated calcium signal transducer 1), and CD326. The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen. CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. The antigen is being used as a target for immunotherapy treatment of human carcinomas. CD326 binds LAIR-1 (CD305) and LAIR-2 (CD306) to inhibit cellular activation and inflammation. This epithelial glycoprotein is now recognized as having an important role in tumor biology.

Applications Reported: This G8.8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This G8.8 antibody has been tested by flow cytometric analysis of the TE-71 cell line. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling.

STAR protocols

Ge Y,Zadeh M,Mohamadzadeh M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Mar 17 00:00:00 EDT 2023

Major depression-related factor NEGR1 controls salivary secretion in mouse submandibular glands.

iScience

Lee J,Kim S,Lee B,Kim YB,Kim KH,Chung G,Lee SJ,Lee S,Sun W,Park HK,Choi SY

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri May 19 00:00:00 EDT 2023

Endothelial AHR activity prevents lung barrier disruption in viral infection.

Nature

Major J,Crotta S,Finsterbusch K,Chakravarty P,Shah K,Frederico B,D'Antuono R,Green M,Meader L,Suarez-Bonnet A,Priestnall S,Stockinger B,Wack A

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Generation of liver mesenchyme and ductal cell organoid co-culture using cell self-aggregation and droplet microfluidics.

STAR protocols

Dowbaj AM,Kohler TN,Cordero-Espinoza L,Hollfelder F,Huch M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Jun 02 00:00:00 EDT 2023

Developmental diversity and unique sensitivity to injury of lung endothelial subtypes during postnatal growth.

iScience

Zanini F,Che X,Knutsen C,Liu M,Suresh NE,Domingo-Gonzalez R,Dou SH,Zhang D,Pryhuber GS,Jones RC,Quake SR,Cornfield DN,Alvira CM

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Mar 17 00:00:00 EDT 2023

Enteric viruses replicate in salivary glands and infect through saliva.

Nature

Ghosh S,Kumar M,Santiana M,Mishra A,Zhang M,Labayo H,Chibly AM,Nakamura H,Tanaka T,Henderson W,Lewis E,Voss O,Su Y,Belkaid Y,Chiorini JA,Hoffman MP,Altan-Bonnet N

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2022

IL-33 induces thymic involution-associated naive T cell aging and impairs host control of severe infection.

Nature communications

Xu L,Wei C,Chen Y,Wu Y,Shou X,Chen W,Lu D,Sun H,Li W,Yu B,Wang X,Zhang X,Yu Y,Lei Z,Tang R,Zhu J,Li Y,Lu L,Zhou H,Zhou S,Su C,Chen X

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Sat Nov 12 00:00:00 EST 2022

A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands.

Cell reports

Mauduit O,Aure MH,Delcroix V,Basova L,Srivastava A,Umazume T,Mays JW,Bellusci S,Tucker AS,Hajihosseini MK,Hoffman MP,Makarenkova HP

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Tue Apr 12 00:00:00 EDT 2022

Oncolytic vaccinia virus expressing a bispecific T-cell engager enhances immune responses in EpCAM positive solid tumors.

Frontiers in immunology

Wei M,Zuo S,Chen Z,Qian P,Zhang Y,Kong L,Gao H,Wei J,Dong J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Dec 02 00:00:00 EST 2022

Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity.

Science advances

Kumar B,Adebayo AK,Prasad M,Capitano ML,Wang R,Bhat-Nakshatri P,Anjanappa M,Simpson E,Chen D,Liu Y,Schilder JM,Colter AB,Maguire C,Temm CJ,Sandusky G,Doud EH,Wijeratne AB,Mosley AL,Broxmeyer HE,Nakshatri H

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Jan 14 00:00:00 EST 2022

Cells of the human intestinal tract mapped across space and time.

Nature

Elmentaite R,Kumasaka N,Roberts K,Fleming A,Dann E,King HW,Kleshchevnikov V,Dabrowska M,Pritchard S,Bolt L,Vieira SF,Mamanova L,Huang N,Perrone F,Goh Kai'En I,Lisgo SN,Katan M,Leonard S,Oliver TRW,Hook CE,Nayak K,Campos LS,Domínguez Conde C,Stephenson E,Engelbert J,Botting RA,Polanski K,van Dongen S,Patel M,Morgan MD,Marioni JC,Bayraktar OA,Meyer KB,He X,Barker RA,Uhlig HH,Mahbubani KT,Saeb-Parsy K,Zilbauer M,Clatworthy MR,Haniffa M,James KR,Teichmann SA

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation.

Cell stem cell

Cordero-Espinoza L,Dowbaj AM,Kohler TN,Strauss B,Sarlidou O,Belenguer G,Pacini C,Martins NP,Dobie R,Wilson-Kanamori JR,Butler R,Prior N,Serup P,Jug F,Henderson NC,Hollfelder F,Huch M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Thu Nov 04 00:00:00 EDT 2021

Gut epithelial IL-27 confers intestinal immunity through the induction of intraepithelial lymphocytes.

The Journal of experimental medicine

Lin CH,Chen MC,Lin LL,Christian DA,Min B,Hunter CA,Lu LF

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12.

Molecular therapy : the journal of the American Society of Gene Therapy

Teng Y,Xu F,Zhang X,Mu J,Sayed M,Hu X,Lei C,Sriwastva M,Kumar A,Sundaram K,Zhang L,Park JW,Chen SY,Zhang S,Yan J,Merchant ML,Zhang X,McClain CJ,Wolfe JK,Adcock RS,Chung D,Palmer KE,Zhang HG

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Wed Aug 04 00:00:00 EDT 2021

Progastrin production transitions from Bmi1⁺/Prox1⁺ to Lgr5^high cells during early intestinal tumorigenesis.

Translational oncology

Giraud J,Foroutan M,Boubaker-Vitre J,Grillet F,Homayed Z,Jadhav U,Crespy P,Breuker C,Bourgaux JF,Hazerbroucq J,Pignodel C,Brulin B,Shivdasani RA,Jay P,Hollande F,Pannequin J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

miR-802 regulates Paneth cell function and enterocyte differentiation in the mouse small intestine.

Nature communications

Goga A,Yagabasan B,Herrmanns K,Godbersen S,Silva PN,Denzler R,Zünd M,Furter M,Schwank G,Sunagawa S,Hardt WD,Stoffel M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Mon Jun 07 00:00:00 EDT 2021

Isolation and culture of mouse alveolar type II cells to study type II to type I cell differentiation.

STAR protocols

Chen Q,Liu Y

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

Evolution of fibroblasts in the lung metastatic microenvironment is driven by stage-specific transcriptional plasticity.

eLife

Shani O,Raz Y,Monteran L,Scharff Y,Levi-Galibov O,Megides O,Shacham H,Cohen N,Silverbush D,Avivi C,Sharan R,Madi A,Scherz-Shouval R,Barshack I,Tsarfaty I,Erez N

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas.

Science advances

Heino S,Fang S,Lähde M,Högström J,Nassiri S,Campbell A,Flanagan D,Raven A,Hodder M,Nasreddin N,Xue HH,Delorenzi M,Leedham S,Petrova TV,Sansom O,Alitalo K

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Nov 19 00:00:00 EST 2021

Comparative analysis of cell lineage differentiation during hepatogenesis in humans and mice at the single-cell transcriptome level.

Cell research

Wang X,Yang L,Wang YC,Xu ZR,Feng Y,Zhang J,Wang Y,Xu CR

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

The Cytokine TGF-β Induces Interleukin-31 Expression from Dermal Dendritic Cells to Activate Sensory Neurons and Stimulate Wound Itching.

Immunity

Xu J,Zanvit P,Hu L,Tseng PY,Liu N,Wang F,Liu O,Zhang D,Jin W,Guo N,Han Y,Yin J,Cain A,Hoon MA,Wang S,Chen W

47-5791 was used in Flow cytometry/Cell sorting to investigate the mechanisms underlying itching associated with cutaneous wound healing, focusing on the contribution of soluble factors released during healing.

Tue Aug 18 00:00:00 EDT 2020

Transfer of metastatic traits via miR-200c in extracellular vesicles derived from colorectal cancer stem cells is inhibited by atractylenolide I.

Clinical and translational medicine

Tang D,Xu X,Ying J,Xie T,Cao G

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Impact of a high‑fat diet on intestinal stem cells and epithelial barrier function in middle‑aged female mice.

Molecular medicine reports

Xie Y,Ding F,Di W,Lv Y,Xia F,Sheng Y,Yu J,Ding G

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium.

Genome biology

Kazakevych J,Denizot J,Liebert A,Portovedo M,Mosavie M,Jain P,Stellato C,Fraser C,Corrêa RO,Célestine M,Mattiuz R,Okkenhaug H,Miller JR,Vinolo MAR,Veldhoen M,Varga-Weisz P

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Wed Mar 11 00:00:00 EDT 2020

Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103⁺CD11b⁺ Dendritic Cells.

Frontiers in immunology

Ko HJ,Hong SW,Verma R,Jung J,Lee M,Kim N,Kim D,Surh CD,Kim KS,Rudra D,Im SH

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Mon Apr 19 00:00:00 EDT 2021

Interleukin 27 Protects From Gastric Atrophy and Metaplasia During Chronic Autoimmune Gastritis.

Cellular and molecular gastroenterology and hepatology

Bockerstett KA,Petersen CP,Noto CN,Kuehm LM,Wong CF,Ford EL,Teague RM,Mills JC,Goldenring JR,DiPaolo RJ

47-5791 was used in Flow cytometry/Cell sorting to investigate the role of interleukin 27 (IL27) in the development of gastric atrophy, hyperplasia, and metaplasia (preneoplastic lesions associated with inflammation-induced gastric cancer) in mice with autoimmune gastritis.

Tue Sep 07 00:00:00 EDT 2021

Collagenase Nanoparticles Enhance the Penetration of Drugs into Pancreatic Tumors.

ACS nano

Zinger A,Koren L,Adir O,Poley M,Alyan M,Yaari Z,Noor N,Krinsky N,Simon A,Gibori H,Krayem M,Mumblat Y,Kasten S,Ofir S,Fridman E,Milman N,Lübtow MM,Liba L,Shklover J,Shainsky-Roitman J,Binenbaum Y,Hershkovitz D,Gil Z,Dvir T,Luxenhofer R,Satchi-Fainaro R,Schroeder A

47-5791 was used in Flow cytometry/Cell sorting to investigate the effect of pretreatment with a proteolytic enzyme nanoparticle system disassembles the pancreatic ductal adenocarcinoma collagen stroma and increases drug penetration into the tumout.

Tue Oct 22 00:00:00 EDT 2019

A critical role for miR-142 in alveolar epithelial lineage formation in mouse lung development.

Cellular and molecular life sciences : CMLS

Shrestha A,Carraro G,Nottet N,Vazquez-Armendariz AI,Herold S,Cordero J,Singh I,Wilhelm J,Barreto G,Morty R,El Agha E,Mari B,Chen C,Zhang JS,Chao CM,Bellusci S

47-5791-82 was used in Chromatin immunoprecipitation to investigate if the glucocorticoid-miR-142-Ep300-β-catenin signaling axis controls pneumocyte maturation.

Mon Jul 01 00:00:00 EDT 2019

The MMTV-Wnt1 murine model produces two phenotypically distinct subtypes of mammary tumors with unique therapeutic responses to an EGFR inhibitor.

Disease models & mechanisms

Pfefferle AD,Darr DB,Calhoun BC,Mott KR,Rosen JM,Perou CM

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Jul 05 00:00:00 EDT 2019

Cellular Plasticity of Defa4^Cre-Expressing Paneth Cells in Response to Notch Activation and Intestinal Injury.

Cellular and molecular gastroenterology and hepatology

Jones JC,Brindley CD,Elder NH,Myers MG,Rajala MW,Dekaney CM,McNamee EN,Frey MR,Shroyer NF,Dempsey PJ

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Tue Jun 04 00:00:00 EDT 2019

Procr-expressing progenitor cells are responsible for murine ovulatory rupture repair of ovarian surface epithelium.

Nature communications

Wang J,Wang D,Chu K,Li W,Zeng YA

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Thu Oct 31 00:00:00 EDT 2019

Potent Antitumor Activity of Liposomal Irinotecan in an Organoid- and CRISPR-Cas9-Based Murine Model of Gallbladder Cancer.

Cancers

Erlangga Z,Wolff K,Poth T,Peltzer A,Nahnsen S,Spielberg S,Timrott K,Woller N,Kühnel F,Manns MP,Saborowski A,Vogel A,Saborowski M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Nov 29 00:00:00 EST 2019

Murine Liver Organoids as a Genetically Flexible System to Study Liver Cancer In Vivo and In Vitro.

Hepatology communications

Saborowski A,Wolff K,Spielberg S,Beer B,Hartleben B,Erlangga Z,Becker D,Dow LE,Marhenke S,Woller N,Unger K,Schirmacher P,Manns MP,Marquardt JU,Vogel A,Saborowski M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Lung epithelial cell-derived IL-25 negatively regulates LPS-induced exosome release from macrophages.

Military Medical Research

Li ZG,Scott MJ,Brzóska T,Sundd P,Li YH,Billiar TR,Wilson MA,Wang P,Fan J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Mon Jul 30 00:00:00 EDT 2018

YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration.

Cell stem cell

Yui S,Azzolin L,Maimets M,Pedersen MT,Fordham RP,Hansen SL,Larsen HL,Guiu J,Alves MRP,Rundsten CF,Johansen JV,Li Y,Madsen CD,Nakamura T,Watanabe M,Nielsen OH,Schweiger PJ,Piccolo S,Jensen KB

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Thu Jan 04 00:00:00 EST 2018

High MUC2 Mucin Expression and Misfolding Induce Cellular Stress, Reactive Oxygen Production, and Apoptosis in Goblet Cells.

The American journal of pathology

Tawiah A,Cornick S,Moreau F,Gorman H,Kumar M,Tiwari S,Chadee K

47-5791 was used in Flow cytometry/Cell sorting to study the role of MUC2 in the depletion of goblet cells and mucus layer in inflammatory bowel disease.

Fri Jun 01 00:00:00 EDT 2018

Lymphotoxin α fine-tunes T cell clonal deletion by regulating thymic entry of antigen-presenting cells.

Nature communications

Lopes N,Charaix J,Cédile O,Sergé A,Irla M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Wed Mar 28 00:00:00 EDT 2018

Molecular Characterization of Gastric Epithelial Cells Using Flow Cytometry.

International journal of molecular sciences

Bockerstett KA,Wong CF,Koehm S,Ford EL,DiPaolo RJ

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Apr 06 00:00:00 EDT 2018

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases.

Frontiers in immunology

Glal D,Sudhakar JN,Lu HH,Liu MC,Chiang HY,Liu YC,Cheng CF,Shui JW

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Thu Sep 26 00:00:00 EDT 2019

DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.

Nature

Yamaji M,Jishage M,Meyer C,Suryawanshi H,Der E,Yamaji M,Garzia A,Morozov P,Manickavel S,McFarland HL,Roeder RG,Hafner M,Tuschl T

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Thu Mar 23 00:00:00 EDT 2017

Regulatory T Cells in Skin Facilitate Epithelial Stem Cell Differentiation.

Cell

Ali N,Zirak B,Rodriguez RS,Pauli ML,Truong HA,Lai K,Ahn R,Corbin K,Lowe MM,Scharschmidt TC,Taravati K,Tan MR,Ricardo-Gonzalez RR,Nosbaum A,Bertolini M,Liao W,Nestle FO,Paus R,Cotsarelis G,Abbas AK,Rosenblum MD

47-5791 was used in Flow cytometry/Cell sorting to study the role of regulatory T cells in hair follicles and hair follicle stem cell biology.

Thu Jun 01 00:00:00 EDT 2017

Effects of Orally Ingested Arsenic on Respiratory Epithelial Permeability to Bacteria and Small Molecules in Mice.

Environmental health perspectives

Henderson MW,Madenspacher JH,Whitehead GS,Thomas SY,Aloor JJ,Gowdy KM,Fessler MB

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Thu Sep 28 00:00:00 EDT 2017

An essential role of CBL and CBL-B ubiquitin ligases in mammary stem cell maintenance.

Development (Cambridge, England)

Mohapatra B,Zutshi N,An W,Goetz B,Arya P,Bielecki TA,Mushtaq I,Storck MD,Meza JL,Band V,Band H

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Wed Mar 15 00:00:00 EDT 2017

ABCG1 regulates pulmonary surfactant metabolism in mice and men.

Journal of lipid research

de Aguiar Vallim TQ,Lee E,Merriott DJ,Goulbourne CN,Cheng J,Cheng A,Gonen A,Allen RM,Palladino END,Ford DA,Wang T,Baldán Á,Tarling EJ

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

The C-terminus domain of the hepatitis B virus x protein stimulates the proliferation of mouse foetal hepatic progenitor cells, although it is not required for the formation of spheroids.

International journal of molecular medicine

Yu L,Chen S,Luo N,He S

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Reconstructing Lineage Hierarchies of Mouse Uterus Epithelial Development Using Single-Cell Analysis.

Stem cell reports

Wu B,An C,Li Y,Yin Z,Gong L,Li Z,Liu Y,Heng BC,Zhang D,Ouyang H,Zou X

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Tue Jul 11 00:00:00 EDT 2017

Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry.

Scientific reports

Liu Z,Guo W,Zhang D,Pang Y,Shi J,Wan S,Cheng K,Wang J,Cheng S

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Dec 23 00:00:00 EST 2016

Regulation of Prostate Development and Benign Prostatic Hyperplasia by Autocrine Cholinergic Signaling via Maintaining the Epithelial Progenitor Cells in Proliferating Status.

Stem cell reports

Wang N,Dong BJ,Quan Y,Chen Q,Chu M,Xu J,Xue W,Huang YR,Yang R,Gao WQ

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Tue May 10 00:00:00 EDT 2016

Long-Term In Vitro Expansion of Salivary Gland Stem Cells Driven by Wnt Signals.

Stem cell reports

Maimets M,Rocchi C,Bron R,Pringle S,Kuipers J,Giepmans BN,Vries RG,Clevers H,de Haan G,van Os R,Coppes RP

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2016

LTβR controls thymic portal endothelial cells for haematopoietic progenitor cell homing and T-cell regeneration.

Nature communications

Shi Y,Wu W,Chai Q,Li Q,Hou Y,Xia H,Ren B,Xu H,Guo X,Jin C,Lv M,Wang Z,Fu YX,Zhu M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Fri Aug 05 00:00:00 EDT 2016

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2015

Glycoprotein A33 deficiency: a new mouse model of impaired intestinal epithelial barrier function and inflammatory disease.

Disease models & mechanisms

Williams BB,Tebbutt NC,Buchert M,Putoczki TL,Doggett K,Bao S,Johnstone CN,Masson F,Hollande F,Burgess AW,Scott AM,Ernst M,Heath JK

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

Live fibroblast harvest reveals surface marker shift in vitro.

Tissue engineering. Part C, Methods

Walmsley GG,Rinkevich Y,Hu MS,Montoro DT,Lo DD,McArdle A,Maan ZN,Morrison SD,Duscher D,Whittam AJ,Wong VW,Weissman IL,Gurtner GC,Longaker MT

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2015

Immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes.

Nature medicine

Clatworthy MR,Aronin CE,Mathews RJ,Morgan NY,Smith KG,Germain RN

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Mon Dec 01 00:00:00 EST 2014

Dedifferentiation of committed epithelial cells into stem cells in vivo.

Nature

Tata PR,Mou H,Pardo-Saganta A,Zhao R,Prabhu M,Law BM,Vinarsky V,Cho JL,Breton S,Sahay A,Medoff BD,Rajagopal J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Thu Nov 14 00:00:00 EST 2013

KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development.

Cell stem cell

Goldman O,Han S,Sourisseau M,Dziedzic N,Hamou W,Corneo B,D'Souza S,Sato T,Kotton DN,Bissig KD,Kalir T,Jacobs A,Evans T,Evans MJ,Gouon-Evans V

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Thu Jun 06 00:00:00 EDT 2013

A multicenter study to standardize reporting and analyses of fluorescence-activated cell-sorted murine intestinal epithelial cells.

American journal of physiology. Gastrointestinal and liver physiology

Magness ST,Puthoff BJ,Crissey MA,Dunn J,Henning SJ,Houchen C,Kaddis JS,Kuo CJ,Li L,Lynch J,Martin MG,May R,Niland JC,Olack B,Qian D,Stelzner M,Swain JR,Wang F,Wang J,Wang X,Yan K,Yu J,Wong MH

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Tue Oct 15 00:00:00 EDT 2013

Gli1 deletion prevents Helicobacter-induced gastric metaplasia and expansion of myeloid cell subsets.

PloS one

El-Zaatari M,Kao JY,Tessier A,Bai L,Hayes MM,Fontaine C,Eaton KA,Merchant JL

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Tue Sep 10 00:00:00 EDT 2013

Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment.

Blood

Cook BD,Liu S,Evans T

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 47-5791-82) in Flow Cytometry

Thu Jun 16 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD326; EGP314; Ep-CAM; Epithelial cell adhesion molecule; Epithelial glycoprotein 314; hEGP314; lymphocyte antigen 74; mEGP314; panepithelial glycoprotein 314; Protein 289A; Trop-1 protein; Tumor-associated calcium signal transducer 1

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Gene Aliases: CD326; EGP; EGP-2; Egp314; Ep-CAM; Epcam; EpCAM1; GA733-2; gp40; Ly74; Tacsd1; Tacstd1; TROP1

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UniProt ID: (Mouse) Q99JW5

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Entrez Gene ID: (Mouse) 17075

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Function(s)

protein complex binding cadherin binding involved in cell-cell adhesion

Process(es)

positive regulation of cell proliferation signal transduction involved in regulation of gene expression negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter stem cell differentiation cell-cell adhesion negative regulation of cell-cell adhesion mediated by cadherin positive regulation of cell motility positive regulation of stem cell proliferation

Performance Guarantee

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CD326 (EpCAM) Monoclonal Antibody (G8.8), APC-eFluor™ 780, eBioscience™

CD326 (EpCAM) Antibody (47-5791-82) in Flow

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47-5791-82

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