Invitrogen

IL-13 Monoclonal Antibody (eBio13A), APC-eFluor™ 780, eBioscience™

14 Published Figures

16 References

View all (67) IL-13 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (14)

FIGURE: 1 / 15

IL-13 Antibody (47-7133-82) in Flow

BALB/c splenocytes were stimulated for 3 days with plate-bound Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82), soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82), Mouse IL-2 Recombinant Protein (Product # 14-8021-64), and Mouse IL-4 Recombinant Protein (Product # 14-8041-80). Cells were then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93) for 5 hours. Following restimulation, cells were fixed and permeabilized (Product # 88-8824-00) and then stai... View More

IL-13 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

IL-13 Antibody in Flow Cytometry, ELISA (Flow, ELISA)

Product Image Gallery

Product Details

47-7133-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 14 publications 14 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio13A

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2716964

Product Specific Information

Description: The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing. In cancer models it has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)

Applications Reported: This eBio13A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio13A antibody has been tested by intracellular staining and flow cytometric analysis of Th2-polarized mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Self-amplifying mRNA seasonal influenza vaccines elicit mouse neutralizing antibody and cell-mediated immunity and protect ferrets.

NPJ vaccines

Cheung M,Chang C,Rathnasinghe R,Rossignol E,Zhang Y,Ferrari A,Patel H,Huang Y,Sanchez Guillen M,Scalzo T,Lee C,Otten GR,Settembre EC,Music N,Palladino G,Wen Y

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Wed Oct 04 00:00:00 EDT 2023

Monitoring Group 2 Innate Lymphoid Cell Biology in Models of Lung Inflammation.

Bio-protocol

Badrani JH,Strohm AN,Haung YA,Doherty TA

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Thu Jul 20 00:00:00 EDT 2023

Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice.

Nature communications

Cheong LY,Wang B,Wang Q,Jin L,Kwok KHM,Wu X,Shu L,Lin H,Chung SK,Cheng KKY,Hoo RLC,Xu A

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Fri Mar 03 00:00:00 EST 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Mitochondrial transcription factor A in RORγt⁺ lymphocytes regulate small intestine homeostasis and metabolism.

Nature communications

Fu Z,Dean JW,Xiong L,Dougherty MW,Oliff KN,Chen ZE,Jobin C,Garrett TJ,Zhou L

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Thu Jul 22 00:00:00 EDT 2021

Synergistic effect of non-neutralizing antibodies and interferon-γ for cross-protection against influenza.

iScience

Shibuya M,Tamiya S,Kawai A,Hirai T,Cragg MS,Yoshioka Y

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in ELISA

Fri Oct 22 00:00:00 EDT 2021

Synergistic effect of non-neutralizing antibodies and interferon-γ for cross-protection against influenza.

iScience

Shibuya M,Tamiya S,Kawai A,Hirai T,Cragg MS,Yoshioka Y

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in ELISA

Fri Oct 22 00:00:00 EDT 2021

Type 1 Cytotoxic T Cells Increase in Placenta after Intrauterine Inflammation.

Frontiers in immunology

Liu J,Liu Y,Panda S,Liu A,Lei J,Burd I

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Gut Microbial Metabolite Pravastatin Attenuates Intestinal Ischemia/Reperfusion Injury Through Promoting IL-13 Release From Type II Innate Lymphoid Cells via IL-33/ST2 Signaling.

Frontiers in immunology

Deng F,Hu JJ,Yang X,Sun QS,Lin ZB,Zhao BC,Yao ZW,Luo SD,Chen ZL,Liu Y,Yan ZZ,Li C,Liu WF,Liu KX

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Tue Dec 21 00:00:00 EST 2021

Gut Microbial Metabolite Pravastatin Attenuates Intestinal Ischemia/Reperfusion Injury Through Promoting IL-13 Release From Type II Innate Lymphoid Cells via IL-33/ST2 Signaling.

Frontiers in immunology

Deng F,Hu JJ,Yang X,Sun QS,Lin ZB,Zhao BC,Yao ZW,Luo SD,Chen ZL,Liu Y,Yan ZZ,Li C,Liu WF,Liu KX

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Tue Dec 21 00:00:00 EST 2021

6-Phosphogluconate dehydrogenase (6PGD), a key checkpoint in reprogramming of regulatory T cells metabolism and function.

eLife

Daneshmandi S,Cassel T,Higashi RM,Fan TW,Seth P

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Thu Oct 28 00:00:00 EDT 2021

ILC3s control airway inflammation by limiting T cell responses to allergens and microbes.

Cell reports

Teng F,Tachó-Piñot R,Sung B,Farber DL,Worgall S,Hammad H,Lambrecht BN,Hepworth MR,Sonnenberg GF

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma.

Nature immunology

Harb H,Stephen-Victor E,Crestani E,Benamar M,Massoud A,Cui Y,Charbonnier LM,Arbag S,Baris S,Cunnigham A,Leyva-Castillo JM,Geha RS,Mousavi AJ,Guennewig B,Schmitz-Abe K,Sioutas C,Phipatanakul W,Chatila TA

47-7133 was used in Flow cytometry/Cell sorting to find that interleukin-6- and STAT3 transcription factor-dependent upregulation of Notch4 receptor on lung tissue regulatory T (Treg) cells is necessary for allergens and particulate matter pollutants to promote airway inflammation.

Sun Nov 01 00:00:00 EDT 2020

A helminth-derived suppressor of ST2 blocks allergic responses.

eLife

Vacca F,Chauché C,Jamwal A,Hinchy EC,Heieis G,Webster H,Ogunkanbi A,Sekne Z,Gregory WF,Wear M,Perona-Wright G,Higgins MK,Nys JA,Cohen ES,McSorley HJ

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Mon May 18 00:00:00 EDT 2020

TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity.

Cell reports

Hurrell BP,Galle-Treger L,Jahani PS,Howard E,Helou DG,Banie H,Soroosh P,Akbari O

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Tue Dec 24 00:00:00 EST 2019

Nematode-Infected Mice Acquire Resistance to Subsequent Infection With Unrelated Nematode by Inducing Highly Responsive Group 2 Innate Lymphoid Cells in the Lung.

Frontiers in immunology

Yasuda K,Adachi T,Koida A,Nakanishi K

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody; T-cell activation protein P600

View more View less

Gene Aliases: Il-13; Il13

View more View less

UniProt ID: (Mouse) P20109

View more View less

Entrez Gene ID: (Mouse) 16163

View more View less

Function(s)

cytokine activity cytokine receptor binding interleukin-13 receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-13 Monoclonal Antibody (eBio13A), APC-eFluor™ 780, eBioscience™

IL-13 Antibody (47-7133-82) in Flow

Product Details

47-7133-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!