Invitrogen

CD335 (NKp46) Monoclonal Antibody (29A1.4), eFluor™ 450, eBioscience™

15 Published Figures

21 References

View all (45) NKp46 (CD335) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (15)

CD335 (NKp46) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 16

CD335 (NKp46) Antibody (48-3351-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD49b (Integrin alpha 2) PE (Product # 12-5971-82) and 0.25 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.25 µg of Anti-Mouse CD335 (NKp46) eFluor® 450 (right). Total viable cells were used for analysis.

CD335 (NKp46) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

48-3351-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

29A1.4

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10557245

Product Specific Information

Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.

The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo.

Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Cell-in-cell structure mediates in-cell killing suppressed by CD44.

Cell discovery

Su Y,Huang H,Luo T,Zheng Y,Fan J,Ren H,Tang M,Niu Z,Wang C,Wang Y,Zhang Z,Liang J,Ruan B,Gao L,Chen Z,Melino G,Wang X,Sun Q

Published figure using CD335 (NKp46) monoclonal antibody (Product # 48-3351-82) in Immunohistochemistry (Paraffin)

Tue Apr 19 00:00:00 EDT 2022

Clr-f expression regulates kidney immune and metabolic homeostasis.

Scientific reports

Zein HS,Abou-Samra E,Scur M,Gutsol A,Hall CW,Dasgupta B,Gharibeh L,Abujamel T,Medina-Luna D,Gamage GS,Pelino TJ,Nemer M,Rahim MMA,Steinle A,Parsons BD,Makrigiannis AP

48-3351-82 was used in Immunohistochemistry to reveal a role for Clr-f in the maintenance of kidney immune and metabolic homeostasis.

Tue Mar 22 00:00:00 EDT 2022

NK cells in hypoxic skin mediate a trade-off between wound healing and antibacterial defence.

Nature communications

Sobecki M,Krzywinska E,Nagarajan S,Audigé A,Hu¿nh K,Zacharjasz J,Debbache J,Kerdiles Y,Gotthardt D,Takeda N,Fandrey J,Sommer L,Sexl V,Stockmann C

Published figure using CD335 (NKp46) monoclonal antibody (Product # 48-3351-82) in Flow Cytometry

Wed Aug 04 00:00:00 EDT 2021

Interferon Lambda Regulates Cellular and Humoral Immunity in Pristane-Induced Lupus.

International journal of molecular sciences

Aschman T,Schaffer S,Biniaris Georgallis SI,Triantafyllopoulou A,Staeheli P,Voll RE

Published figure using CD335 (NKp46) monoclonal antibody (Product # 48-3351-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

Flow cytometry-based ex vivo murine NK cell cytotoxicity assay.

STAR protocols

Wong P,Wagner JA,Berrien-Elliott MM,Schappe T,Fehniger TA

Published figure using CD335 (NKp46) monoclonal antibody (Product # 48-3351-82) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

Characterization of the pathoimmunology of necrotizing enterocolitis reveals novel therapeutic opportunities.

Nature communications

Cho SX,Rudloff I,Lao JC,Pang MA,Goldberg R,Bui CB,McLean CA,Stock M,Klassert TE,Slevogt H,Mangan NE,Cheng W,Fischer D,Gfroerer S,Sandhu MK,Ngo D,Bujotzek A,Lariviere L,Schumacher F,Tiefenthaler G,Beker F,Collins C,Kamlin COF,König K,Malhotra A,Tan K,Theda C,Veldman A,Ellisdon AM,Whisstock JC,Berger PJ,Nold-Petry CA,Nold MF

Published figure using CD335 (NKp46) monoclonal antibody (Product # 48-3351-82) in Flow Cytometry

Fri Nov 13 00:00:00 EST 2020

Novel CD11b⁺Gr-1⁺Sca-1⁺ myeloid cells drive mortality in bacterial infection.

Science advances

Park MY,Kim HS,Lee HY,Zabel BA,Bae YS

Published figure using CD335 (NKp46) monoclonal antibody (Product # 48-3351-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Natural killer cells limit the clearance of senescent lung adenocarcinoma cells.

Oncogenesis

Stokes KL,Cortez-Retamozo V,Acosta J,Lauderback B,Robles-Oteiza C,Cicchini M,Pittet MJ,Feldser DM

Mon Apr 01 00:00:00 EDT 2019

Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

Published figure using CD335 (NKp46) monoclonal antibody (Product # 48-3351-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

SCGN deficiency results in colitis susceptibility.

eLife

Sifuentes-Dominguez LF,Li H,Llano E,Liu Z,Singla A,Patel AS,Kathania M,Khoury A,Norris N,Rios JJ,Starokadomskyy P,Park JY,Gopal P,Liu Q,Tan S,Chan L,Ross T,Harrison S,Venuprasad K,Baker LA,Jia D,Burstein E

Published figure using CD335 (NKp46) monoclonal antibody (Product # 48-3351-82) in Flow Cytometry

Wed Oct 30 00:00:00 EDT 2019

DC subset-specific induction of T cell responses upon antigen uptake via Fcγ receptors in vivo.

The Journal of experimental medicine

Lehmann CHK,Baranska A,Heidkamp GF,Heger L,Neubert K,Lühr JJ,Hoffmann A,Reimer KC,Brückner C,Beck S,Seeling M,Kießling M,Soulat D,Krug AB,Ravetch JV,Leusen JHW,Nimmerjahn F,Dudziak D

48-3351 was used in Flow cytometry/Cell sorting to suggest that targeting antigens to FcγRs on DCs may be a promising approach to raise immunogenic or tolerogenic T cell responses to antigens of choice.

Mon May 01 00:00:00 EDT 2017

FHL2 Regulates Natural Killer Cell Development and Activation during Streptococcus pneumoniae Infection.

Frontiers in immunology

Baranek T,Morello E,Valayer A,Aimar RF,Bréa D,Henry C,Besnard AG,Dalloneau E,Guillon A,Dequin PF,Narni-Mancinelli E,Vivier E,Laurent F,Wei Y,Paget C,Si-Tahar M

48-3351 was used in Flow cytometry/Cell sorting to determine the expression and role of FHL2 in natural killer cell biology.

Wed Nov 20 00:00:00 EST 2019

Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 ^iCreTbx21 ^fl/fl mice.

Wellcome open research

Cuff AO,Male V

Published figure using CD335 (NKp46) monoclonal antibody (Product # 48-3351-82) in Flow Cytometry

Sat Jan 09 00:00:00 EST 2021

Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis.

Nature communications

Krzywinska E,Kantari-Mimoun C,Kerdiles Y,Sobecki M,Isagawa T,Gotthardt D,Castells M,Haubold J,Millien C,Viel T,Tavitian B,Takeda N,Fandrey J,Vivier E,Sexl V,Stockmann C

Published figure using CD335 (NKp46) monoclonal antibody (Product # 48-3351-82) in Flow Cytometry

Fri Nov 17 00:00:00 EST 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD335 (NKp46) monoclonal antibody (Product # 48-3351-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

Published figure using CD335 (NKp46) monoclonal antibody (Product # 48-3351-82) in Flow Cytometry

Wed Sep 07 00:00:00 EDT 2016

Inhibition of the CXCL12/CXCR4 chemokine axis with AMD3100, a CXCR4 small molecule inhibitor, worsens murine hepatic injury.

Hepatology research : the official journal of the Japan Society of Hepatology

Saiman Y,Jiao J,Fiel MI,Friedman SL,Aloman C,Bansal MB

48-3351 was used in Flow cytometry/Cell sorting to suggest that inhibition of the CXCR4/CXCL12 chemokine axis is injurious through modulation of the hepatic inflammatory response.

Wed Jul 01 00:00:00 EDT 2015

Endothelial Gata5 transcription factor regulates blood pressure.

Nature communications

Messaoudi S,He Y,Gutsol A,Wight A,Hébert RL,Vilmundarson RO,Makrigiannis AP,Chalmers J,Hamet P,Tremblay J,McPherson R,Stewart AFR,Touyz RM,Nemer M

48-3351 was used in Flow cytometry/Cell sorting to study the aetiology of human hypertension, showing that GATA5, an endothelial transcription factor, regulates blood pressure, allowing the development of a new animal model of hypertension for further studies.

Mon Nov 30 00:00:00 EST 2015

HDAC6 contributes to pathological responses of heart and skeletal muscle to chronic angiotensin-II signaling.

American journal of physiology. Heart and circulatory physiology

Demos-Davies KM,Ferguson BS,Cavasin MA,Mahaffey JH,Williams SM,Spiltoir JI,Schuetze KB,Horn TR,Chen B,Ferrara C,Scellini B,Piroddi N,Tesi C,Poggesi C,Jeong MY,McKinsey TA

48-3351 was used in Flow cytometry/Cell sorting to define novel roles for HDAC6 in striated muscle and demonstrate potential for HDAC6-selective inhibitors for the treatment of cardiac dysfunction.

Tue Jul 15 00:00:00 EDT 2014

Markers of nonselective and specific NK cell activation.

Journal of immunology (Baltimore, Md. : 1950)

Fogel LA,Sun MM,Geurs TL,Carayannopoulos LN,French AR

48-3351 was used in Flow cytometry/Cell sorting to demonstrate that a combination of Sca-1, CD27, and KLRG1 can distinguish NK cells based on their mechanism of activation.

Sat Jun 15 00:00:00 EDT 2013

IL-22 bridges the lymphotoxin pathway with the maintenance of colonic lymphoid structures during infection with Citrobacter rodentium.

Nature immunology

Ota N,Wong K,Valdez PA,Zheng Y,Crellin NK,Diehl L,Ouyang W

Sun Aug 28 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 1-Mar; Activating receptor 1; CD335; hNKp46; Ly-94; Lymphocyte antigen 94; mAR-1; MAR1 (mouse activating receptor 1); mNKp46; Natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; natural killer cell receptor; NK-p46

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Gene Aliases: Ly94; Ncr1; NKp46

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UniProt ID: (Mouse) Q8C567

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Entrez Gene ID: (Mouse) 17086

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Function(s)

immunoglobulin receptor superfamily

Process(es)

detection of virus defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD335 (NKp46) Monoclonal Antibody (29A1.4), eFluor™ 450, eBioscience™

CD335 (NKp46) Antibody (48-3351-82) in Flow

Product Details

48-3351-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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