Invitrogen

CD335 (NKp46) Monoclonal Antibody (29A1.4), eFluor™ 660, eBioscience™

15 Published Figures

24 References

View all (45) NKp46 (CD335) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (15)

CD335 (NKp46) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 16

CD335 (NKp46) Antibody (50-3351-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD49b (Integrin alpha 2) PE (Product # 12-5971-82) and 0.5 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.5 µg of Anti-Mouse CD335 (NKp46) eFluor® 660 (right). Total viable cells were used for analysis.

CD335 (NKp46) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

50-3351-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

29A1.4

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10598664

Product Specific Information

Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.

The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo.

Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Cell-in-cell structure mediates in-cell killing suppressed by CD44.

Cell discovery

Su Y,Huang H,Luo T,Zheng Y,Fan J,Ren H,Tang M,Niu Z,Wang C,Wang Y,Zhang Z,Liang J,Ruan B,Gao L,Chen Z,Melino G,Wang X,Sun Q

Published figure using CD335 (NKp46) monoclonal antibody (Product # 50-3351-82) in Immunohistochemistry (Paraffin)

Tue Apr 19 00:00:00 EDT 2022

NK cells in hypoxic skin mediate a trade-off between wound healing and antibacterial defence.

Nature communications

Sobecki M,Krzywinska E,Nagarajan S,Audigé A,Hu¿nh K,Zacharjasz J,Debbache J,Kerdiles Y,Gotthardt D,Takeda N,Fandrey J,Sommer L,Sexl V,Stockmann C

Published figure using CD335 (NKp46) monoclonal antibody (Product # 50-3351-82) in Flow Cytometry

Wed Aug 04 00:00:00 EDT 2021

Interferon Lambda Regulates Cellular and Humoral Immunity in Pristane-Induced Lupus.

International journal of molecular sciences

Aschman T,Schaffer S,Biniaris Georgallis SI,Triantafyllopoulou A,Staeheli P,Voll RE

Published figure using CD335 (NKp46) monoclonal antibody (Product # 50-3351-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

Flow cytometry-based ex vivo murine NK cell cytotoxicity assay.

STAR protocols

Wong P,Wagner JA,Berrien-Elliott MM,Schappe T,Fehniger TA

Published figure using CD335 (NKp46) monoclonal antibody (Product # 50-3351-82) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment.

eLife

Nicolas-Boluda A,Vaquero J,Vimeux L,Guilbert T,Barrin S,Kantari-Mimoun C,Ponzo M,Renault G,Deptula P,Pogoda K,Bucki R,Cascone I,Courty J,Fouassier L,Gazeau F,Donnadieu E

50-3351-82 was used in Flow Cytometry to investigate the rate of cancer tumour stiffening of dense extracellular matrix, and how this affects T cell migration.

Wed Jun 09 00:00:00 EDT 2021

Characterization of the pathoimmunology of necrotizing enterocolitis reveals novel therapeutic opportunities.

Nature communications

Cho SX,Rudloff I,Lao JC,Pang MA,Goldberg R,Bui CB,McLean CA,Stock M,Klassert TE,Slevogt H,Mangan NE,Cheng W,Fischer D,Gfroerer S,Sandhu MK,Ngo D,Bujotzek A,Lariviere L,Schumacher F,Tiefenthaler G,Beker F,Collins C,Kamlin COF,König K,Malhotra A,Tan K,Theda C,Veldman A,Ellisdon AM,Whisstock JC,Berger PJ,Nold-Petry CA,Nold MF

50-3351 was used in Flow cytometry/Cell sorting to implicate type 3 cytokines, TLRs and IL-37 as potential targets for novel Necrotizing enterocolitis therapies.

Fri Nov 13 00:00:00 EST 2020

Characterization of the pathoimmunology of necrotizing enterocolitis reveals novel therapeutic opportunities.

Nature communications

50-3351 was used in Flow cytometry/Cell sorting to implicate type 3 cytokines, TLRs and IL-37 as potential targets for novel Necrotizing enterocolitis therapies.

Fri Nov 13 00:00:00 EST 2020

Novel CD11b⁺Gr-1⁺Sca-1⁺ myeloid cells drive mortality in bacterial infection.

Science advances

Park MY,Kim HS,Lee HY,Zabel BA,Bae YS

Published figure using CD335 (NKp46) monoclonal antibody (Product # 50-3351-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

Published figure using CD335 (NKp46) monoclonal antibody (Product # 50-3351-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

SCGN deficiency results in colitis susceptibility.

eLife

Sifuentes-Dominguez LF,Li H,Llano E,Liu Z,Singla A,Patel AS,Kathania M,Khoury A,Norris N,Rios JJ,Starokadomskyy P,Park JY,Gopal P,Liu Q,Tan S,Chan L,Ross T,Harrison S,Venuprasad K,Baker LA,Jia D,Burstein E

Published figure using CD335 (NKp46) monoclonal antibody (Product # 50-3351-82) in Flow Cytometry

Wed Oct 30 00:00:00 EDT 2019

Dysfunction of Natural Killer Cells by FBP1-Induced Inhibition of Glycolysis during Lung Cancer Progression.

Cell metabolism

Cong J,Wang X,Zheng X,Wang D,Fu B,Sun R,Tian Z,Wei H

50-3351 was used in Flow cytometry/Cell sorting to identify novel mechanisms in natural killer cell dysfunction.

Tue Aug 07 00:00:00 EDT 2018

Macrophages impede CD8 T cells from reaching tumor cells and limit the efficacy of anti-PD-1 treatment.

Proceedings of the National Academy of Sciences of the United States of America

Peranzoni E,Lemoine J,Vimeux L,Feuillet V,Barrin S,Kantari-Mimoun C,Bercovici N,Guérin M,Biton J,Ouakrim H,Régnier F,Lupo A,Alifano M,Damotte D,Donnadieu E

50-3351 was used in Flow cytometry/Cell sorting to show that exclusion of CD8 T cells from tumour islets is correlated with a poor clinical outcome and low lymphocyte motility in human lung squamous-cell carcinomas.

Tue Apr 24 00:00:00 EDT 2018

Interleukin-33-Activated Islet-Resident Innate Lymphoid Cells Promote Insulin Secretion through Myeloid Cell Retinoic Acid Production.

Immunity

Dalmas E,Lehmann FM,Dror E,Wueest S,Thienel C,Borsigova M,Stawiski M,Traunecker E,Lucchini FC,Dapito DH,Kallert SM,Guigas B,Pattou F,Kerr-Conte J,Maechler P,Girard JP,Konrad D,Wolfrum C,Böni-Schnetzler M,Finke D,Donath MY

50-3351 was used in Flow cytometry/Cell sorting to provide evidence that an immunometabolic crosstalk between islet-derived IL-33, ILC2s, and myeloid cells fosters insulin secretion.

Tue Nov 21 00:00:00 EST 2017

Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 ^iCreTbx21 ^fl/fl mice.

Wellcome open research

Cuff AO,Male V

Published figure using CD335 (NKp46) monoclonal antibody (Product # 50-3351-82) in Flow Cytometry

Sat Jan 09 00:00:00 EST 2021

Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis.

Nature communications

Krzywinska E,Kantari-Mimoun C,Kerdiles Y,Sobecki M,Isagawa T,Gotthardt D,Castells M,Haubold J,Millien C,Viel T,Tavitian B,Takeda N,Fandrey J,Vivier E,Sexl V,Stockmann C

Published figure using CD335 (NKp46) monoclonal antibody (Product # 50-3351-82) in Flow Cytometry

Fri Nov 17 00:00:00 EST 2017

Inhibition of arginase by CB-1158 blocks myeloid cell-mediated immune suppression in the tumor microenvironment.

Journal for immunotherapy of cancer

Steggerda SM,Bennett MK,Chen J,Emberley E,Huang T,Janes JR,Li W,MacKinnon AL,Makkouk A,Marguier G,Murray PJ,Neou S,Pan A,Parlati F,Rodriguez MLM,Van de Velde LA,Wang T,Works M,Zhang J,Zhang W,Gross MI

50-3351-82 was used in Flow Cytometry to demonstrate key role of arginase 1 (Arg1), characteristically expressed among myeloid cells in tumors, as a mediator of immune suppression; and explore the therapeutic potential of CB-1158 as a potent inhibitor of Arg1.

Tue Dec 19 00:00:00 EST 2017

Type I IFN and not TNF, is Essential for Cyclic Di-nucleotide-elicited CTL by a Cytosolic Cross-presentation Pathway.

EBioMedicine

Lirussi D,Ebensen T,Schulze K,Trittel S,Duran V,Liebich I,Kalinke U,Guzmán CA

50-3351 was used in Immunocytochemistry-immunoflourescence to demonstrate that stimulation of interferon-γ-secreting CD8+ cytotoxic T lymphocytes is significantly decreased after vaccination in the absence of type I interferon signalling.

Tue Aug 01 00:00:00 EDT 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD335 (NKp46) monoclonal antibody (Product # 50-3351-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Flt3 Ligand Regulates the Development of Innate Lymphoid Cells in Fetal and Adult Mice.

Journal of immunology (Baltimore, Md. : 1950)

Baerenwaldt A,von Burg N,Kreuzaler M,Sitte S,Horvath E,Peter A,Voehringer D,Rolink AG,Finke D

50-3351 was used in Flow cytometry/Cell sorting to investigate the role of the Flt3 ligand in the development and regulation of innate lymphoid cells in fetal and adult mice.

Tue Mar 15 00:00:00 EDT 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

Published figure using CD335 (NKp46) monoclonal antibody (Product # 50-3351-82) in Flow Cytometry

Wed Sep 07 00:00:00 EDT 2016

Activated NKT cells imprint NK-cell differentiation, functionality and education.

European journal of immunology

Riese P,Trittel S,May T,Cicin-Sain L,Chambers BJ,Guzmán CA

50-3351 was used in Flow cytometry/Cell sorting to study the cellular cross-talk between natural killer T cells and natural killer cells.

Mon Jun 01 00:00:00 EDT 2015

Diverse roles for T-bet in the effector responses required for resistance to infection.

Journal of immunology (Baltimore, Md. : 1950)

Harms Pritchard G,Hall AO,Christian DA,Wagage S,Fang Q,Muallem G,John B,Glatman Zaretsky A,Dunn WG,Perrigoue J,Reiner SL,Hunter CA

50-3351 was used in Flow cytometry/Cell sorting to highlight T-bet-independent pathways to IFN-γ production and reveal a novel role for this transcription factor in coordinating the T cell responses necessary to control infection in peripheral tissues.

Sun Feb 01 00:00:00 EST 2015

mTORC1-dependent metabolic reprogramming is a prerequisite for NK cell effector function.

Journal of immunology (Baltimore, Md. : 1950)

Donnelly RP,Loftus RM,Keating SE,Liou KT,Biron CA,Gardiner CM,Finlay DK

50-3351 was used in Flow cytometry/Cell sorting to investigate whether mTORC1-controlled metabolism underpins normal natural killer cell proinflammatory functions.

Sat Nov 01 00:00:00 EDT 2014

Neonatal rhinovirus infection induces mucous metaplasia and airways hyperresponsiveness.

Journal of immunology (Baltimore, Md. : 1950)

Schneider D,Hong JY,Popova AP,Bowman ER,Linn MJ,McLean AM,Zhao Y,Sonstein J,Bentley JK,Weinberg JB,Lukacs NW,Curtis JL,Sajjan US,Hershenson MB

50-3351 was used in Flow cytometry/Cell sorting to conclude that neonatal RV1B infection leads to persistent airway inflammation, in part mediated by IL-13.

Thu Mar 15 00:00:00 EDT 2012

IL-22 bridges the lymphotoxin pathway with the maintenance of colonic lymphoid structures during infection with Citrobacter rodentium.

Nature immunology

Ota N,Wong K,Valdez PA,Zheng Y,Crellin NK,Diehl L,Ouyang W

Sun Aug 28 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 1-Mar; Activating receptor 1; CD335; hNKp46; Ly-94; Lymphocyte antigen 94; mAR-1; MAR1 (mouse activating receptor 1); mNKp46; Natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; natural killer cell receptor; NK-p46

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Gene Aliases: Ly94; Ncr1; NKp46

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UniProt ID: (Mouse) Q8C567

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Entrez Gene ID: (Mouse) 17086

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Function(s)

immunoglobulin receptor superfamily

Process(es)

detection of virus defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD335 (NKp46) Monoclonal Antibody (29A1.4), eFluor™ 660, eBioscience™

CD335 (NKp46) Antibody (50-3351-82) in Flow

Product Details

50-3351-82

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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