Invitrogen

AIRE Monoclonal Antibody (5H12), eFluor™ 660, eBioscience™

19 Published Figures

20 References

View all (25) AIRE antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (19)

AIRE Antibody in Immunohistochemistry (Frozen) (IHC (F))

FIGURE: 1 / 20

AIRE Antibody (50-5934-82) in IHC (F)

Immunohistochemistry on frozen 5-week-old mouse thymus using 10 µg/mL of Anti-Mouse AIRE eFluor® 660. Nuclei are counterstained with DAPI.

AIRE Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

AIRE Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

AIRE Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

50-5934-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

Immunocytochemistry (ICC/IF)

10 µg/mL

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-Dependent

View 9 publications 9 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2c, kappa

Class

Monoclonal

Type

Antibody

Clone

5H12

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574257

Product Specific Information

Description: This 5H12 monoclonal antibody reacts with mouse Autoimmune Regulator (AIRE). This 58-kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs). However, expression of AIRE protein in the periphery remains unclear despite the detection of AIRE mRNA in the lymph node, lymphoid stromal cells, monocytes, and dendritic cells. Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection. Other biological functions for AIRE include chemokine expression and antigen presentation.

The 5H12 antibody recognizes mouse AIRE isoforms 1 and 2.

Applications Reported: This 5H12 antibody has been reported for use in intracellular flow cytometric analysis and immunohistochemical staining of frozen tissue sections.

Applications Tested: This 5H12 antibody has been tested by immunohistochemistry on frozen 5-week-old mouse thymus and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

AIRE is a transcriptional regulator protein that binds to DNA as a dimer and tetramer, but not as a monomer, and may be involved in the regulation of gene expression. A nuclear protein, AIRE is a 545 amino acid protein with an HSR domain, two PHD type zinc fingers, and a SAND domain, and may be present in soluble high-molecular-weight complexes. It may form homodimer, homotetramer and also nuclear bodies and interacts with the transcriptional coactivator CBP and CREBBP. Three isoforms of AIRE are reported and one of them is predicted to be a candidate for nuclear-mediated decay (NMD). AIRE is involved in immune regulation and is known to mediate E3 ubiquitin ligase activity. Defects in AIRE are responsible for the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Embryonic keratin19⁺ progenitors generate multiple functionally distinct progeny to maintain epithelial diversity in the adult thymus medulla.

Nature communications

Lucas B,White AJ,Klein F,Veiga-Villauriz C,Handel A,Bacon A,Cosway EJ,James KD,Parnell SM,Ohigashi I,Takahama Y,Jenkinson WE,Hollander GA,Lu WY,Anderson G

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Flow Cytometry

Wed Apr 12 00:00:00 EDT 2023

Fine-tuning of β-catenin in mouse thymic epithelial cells is required for postnatal T-cell development.

eLife

Fujimori S,Ohigashi I,Abe H,Matsushita Y,Katagiri T,Taketo MM,Takahama Y,Takada S

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Immunohistochemistry

Wed Jan 19 00:00:00 EST 2022

Mechanistic dissection of dominant AIRE mutations in mouse models reveals AIRE autoregulation.

The Journal of experimental medicine

Goldfarb Y,Givony T,Kadouri N,Dobeš J,Peligero-Cruz C,Zalayat I,Damari G,Dassa B,Ben-Dor S,Gruper Y,Oftedal BE,Bratland E,Erichsen MM,Berger A,Avin A,Nevo S,Haljasorg U,Kuperman Y,Ulman A,Haffner-Krausz R,Porat Z,Atasoy U,Leshkowitz D,Husebye ES,Abramson J

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Sirt6 Regulates the Development of Medullary Thymic Epithelial Cells and Contributes to the Establishment of Central Immune Tolerance.

Frontiers in cell and developmental biology

Zhang Q,Liang Z,Zhang J,Lei T,Dong X,Su H,Chen Y,Zhang Z,Tan L,Zhao Y

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Flow Cytometry

Wed Apr 21 00:00:00 EDT 2021

Defective dystrophic thymus determines degenerative changes in skeletal muscle.

Nature communications

Farini A,Sitzia C,Villa C,Cassani B,Tripodi L,Legato M,Belicchi M,Bella P,Lonati C,Gatti S,Cerletti M,Torrente Y

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Immunocytochemistry

Thu Apr 08 00:00:00 EDT 2021

Defective dystrophic thymus determines degenerative changes in skeletal muscle.

Nature communications

Farini A,Sitzia C,Villa C,Cassani B,Tripodi L,Legato M,Belicchi M,Bella P,Lonati C,Gatti S,Cerletti M,Torrente Y

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Immunocytochemistry

Thu Apr 08 00:00:00 EDT 2021

ILC3s control airway inflammation by limiting T cell responses to allergens and microbes.

Cell reports

Teng F,Tachó-Piñot R,Sung B,Farber DL,Worgall S,Hammad H,Lambrecht BN,Hepworth MR,Sonnenberg GF

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

Retracing the evolutionary emergence of thymopoiesis.

Science advances

Swann JB,Nusser A,Morimoto R,Nagakubo D,Boehm T

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Immunohistochemistry

Sun Nov 01 00:00:00 EDT 2020

Trans-omics Impact of Thymoproteasome in Cortical Thymic Epithelial Cells.

Cell reports

Ohigashi I,Tanaka Y,Kondo K,Fujimori S,Kondo H,Palin AC,Hoffmann V,Kozai M,Matsushita Y,Uda S,Motosugi R,Hamazaki J,Kubota H,Murata S,Tanaka K,Katagiri T,Kosako H,Takahama Y

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Immunohistochemistry

Tue Nov 26 00:00:00 EST 2019

MTOR signaling is essential for the development of thymic epithelial cells and the induction of central immune tolerance.

Autophagy

Liang Z,Zhang L,Su H,Luan R,Na N,Sun L,Zhao Y,Zhang X,Zhang Q,Li J,Zhang L,Zhao Y

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Flow Cytometry

Fri Mar 22 00:00:00 EDT 2019

CCR7 defines a precursor for murine iNKT cells in thymus and periphery.

eLife

Wang H,Hogquist KA

50-5934 was used in Flow cytometry/Cell sorting to demonstrate that the thymus is both a source of iNKT progenitors and a site of tissue dependent effector cell differentiation.

Mon Aug 13 00:00:00 EDT 2018

The transcriptional regulator Aire binds to and activates super-enhancers.

Nature immunology

Bansal K,Yoshida H,Benoist C,Mathis D

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Western Blot

Wed Mar 01 00:00:00 EST 2017

The transcriptional regulator Aire binds to and activates super-enhancers.

Nature immunology

Bansal K,Yoshida H,Benoist C,Mathis D

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Western Blot

Wed Mar 01 00:00:00 EST 2017

Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma.

The Journal of experimental medicine

Takahashi T,Asano Y,Sugawara K,Yamashita T,Nakamura K,Saigusa R,Ichimura Y,Toyama T,Taniguchi T,Akamata K,Noda S,Yoshizaki A,Tsuruta D,Trojanowska M,Sato S

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Immunohistochemistry

Mon Apr 03 00:00:00 EDT 2017

A human PSMB11 variant affects thymoproteasome processing and CD8+ T cell production.

JCI insight

Ohigashi I,Ohte Y,Setoh K,Nakase H,Maekawa A,Kiyonari H,Hamazaki Y,Sekai M,Sudo T,Tabara Y,Sawai H,Omae Y,Yuliwulandari R,Tanaka Y,Mizokami M,Inoue H,Kasahara M,Minato N,Tokunaga K,Tanaka K,Matsuda F,Murata S,Takahama Y

50-5934 was used in Immunofluorescence to show that a human genomic PSMB11 variation alters the amino acid sequence of β5t which affects the processing of catalytically active protein.

Thu May 18 00:00:00 EDT 2017

Thymic involution perturbs negative selection leading to autoreactive T cells that induce chronic inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Coder BD,Wang H,Ruan L,Su DM

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

Osteoprotegerin-Mediated Homeostasis of Rank+ Thymic Epithelial Cells Does Not Limit Foxp3+ Regulatory T Cell Development.

Journal of immunology (Baltimore, Md. : 1950)

McCarthy NI,Cowan JE,Nakamura K,Bacon A,Baik S,White AJ,Parnell SM,Jenkinson EJ,Jenkinson WE,Anderson G

50-5934 was used in Flow cytometry/Cell sorting to elucidate the conditions controlling medullary thymic epithelial cell (mTEC) homeostasis and the production of mTEC-dependent Foxp3(+) Tregs.

Tue Sep 15 00:00:00 EDT 2015

The transcriptional regulator Aire coopts the repressive ATF7ip-MBD1 complex for the induction of immunotolerance.

Nature immunology

Waterfield M,Khan IS,Cortez JT,Fan U,Metzger T,Greer A,Fasano K,Martinez-Llordella M,Pollack JL,Erle DJ,Su M,Anderson MS

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Immunofluorescence

Sat Mar 01 00:00:00 EST 2014

The transcriptional regulator Aire coopts the repressive ATF7ip-MBD1 complex for the induction of immunotolerance.

Nature immunology

Waterfield M,Khan IS,Cortez JT,Fan U,Metzger T,Greer A,Fasano K,Martinez-Llordella M,Pollack JL,Erle DJ,Su M,Anderson MS

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Immunofluorescence

Sat Mar 01 00:00:00 EST 2014

RANK signals from CD4(+)3(-) inducer cells regulate development of Aire-expressing epithelial cells in the thymic medulla.

The Journal of experimental medicine

Rossi SW,Kim MY,Leibbrandt A,Parnell SM,Jenkinson WE,Glanville SH,McConnell FM,Scott HS,Penninger JM,Jenkinson EJ,Lane PJ,Anderson G

Published figure using AIRE monoclonal antibody (Product # 50-5934-82) in Immunofluorescence

Mon Jun 11 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: AIRE protein; APECED protein homolog; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog; Autoimmune regulator; autoimmune regulator AIRE1a; OTTHUMP00000109529

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Gene Aliases: Aire

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UniProt ID: (Mouse) Q9Z0E3

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Entrez Gene ID: (Mouse) 11634

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding DNA binding chromatin binding zinc ion binding histone binding identical protein binding transcription regulatory region DNA binding translation regulator activity metal ion binding

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated humoral immune response positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AIRE Monoclonal Antibody (5H12), eFluor™ 660, eBioscience™

AIRE Antibody (50-5934-82) in IHC (F)

Product Details

50-5934-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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