Invitrogen

CD34 Monoclonal Antibody (RAM34), Alexa Fluor™ 488, eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (90)

FIGURE: 1 / 91

CD34 Antibody (53-0341-82) in Flow

C57BL/6 mouse bone marrow cells were stained with CD117 (c-Kit) Monoclonal Antibody, PE-eFluor 610 (Product # 61-1171-82), Mouse Hematopoietic Lineage Antibody Cocktail, eFluor 450 (Product # 88-7772-72), and 0.5 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 488 (Product # 53-4321-80) (left) or 0.5 µg of CD34 Monoclonal Antibody, Alexa Fluor 488 (right). Lineage negative cells were used for analysis.

CD34 Antibody in Immunocytochemistry (ICC/IF)

CD34 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD34 Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD34 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

53-0341-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 12 publications 12 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 7 publications 7 publications

Flow Cytometry (Flow)

1.0 µg/test

View 53 publications 53 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RAM34

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2866440

Product Specific Information

Description: The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin. It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells. CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity. CD34 expressed on endothelial cells is a ligand for CD62L and plays a role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers.

Applications Reported: This RAM34 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RAM34 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Target Information

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression.

International journal of molecular sciences

Li N,Wang Y,Wang A,Zhang J,Jia C,Yu C,Song Z,Wang S,Liu L,Yi J,Bao Y,Huang Y,Sun L

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Fri Feb 03 00:00:00 EST 2023

Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.

Cell

Tarazi S,Aguilera-Castrejon A,Joubran C,Ghanem N,Ashouokhi S,Roncato F,Wildschutz E,Haddad M,Oldak B,Gomez-Cesar E,Livnat N,Viukov S,Lokshtanov D,Naveh-Tassa S,Rose M,Hanna S,Raanan C,Brenner O,Kedmi M,Keren-Shaul H,Lapidot T,Maza I,Novershtern N,Hanna JH

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2022

Multiple benign fibrous histiocytomas of the mandible: A case report and review of the literature.

Experimental and therapeutic medicine

Wang Y,Huang Y,Cai WX,Tao Q

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunohistochemistry

Thu Sep 01 00:00:00 EDT 2022

Glomerular endothelial cell senescence drives age-related kidney disease through PAI-1.

EMBO molecular medicine

Cohen C,Le Goff O,Soysouvanh F,Vasseur F,Tanou M,Nguyen C,Amrouche L,Le Guen J,Saltel-Fulero O,Meunier T,Nguyen-Khoa T,Rabant M,Nochy D,Legendre C,Friedlander G,Childs BG,Baker DJ,Knebelmann B,Anglicheau D,Milliat F,Terzi F

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunocytochemistry

Mon Nov 08 00:00:00 EST 2021

Induction of osteogenic differentiation of bone marrow stromal cells on 3D polyester-based scaffolds solely by subphysiological fluidic stimulation in a laminar flow bioreactor.

Journal of tissue engineering

Yamada S,Yassin MA,Schwarz T,Hansmann J,Mustafa K

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

Functional damage of endothelial progenitor cells is attenuated by 14-3-3-n through inhibition of mitochondrial injury and oxidative stress.

Cell biology international

Li Y,Sun X,Zhang X,Zhou H,Wang D,Xia Y,Li X

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunocytochemistry

Thu Apr 01 00:00:00 EDT 2021

Functional damage of endothelial progenitor cells is attenuated by 14-3-3-n through inhibition of mitochondrial injury and oxidative stress.

Cell biology international

Li Y,Sun X,Zhang X,Zhou H,Wang D,Xia Y,Li X

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunocytochemistry

Thu Apr 01 00:00:00 EDT 2021

Functional Specificity of the Members of the Sos Family of Ras-GEF Activators: Novel Role of Sos2 in Control of Epidermal Stem Cell Homeostasis.

Cancers

Baltanás FC,Mucientes-Valdivieso C,Lorenzo-Martín LF,Fernández-Parejo N,García-Navas R,Segrelles C,Calzada N,Fuentes-Mateos R,Paramio JM,Bustelo XR,Santos E

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Apr 29 00:00:00 EDT 2021

Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration.

PLoS genetics

Flora P,Li MY,Galbo PM,Astorkia M,Zheng D,Ezhkova E

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunohistochemistry

Wed Dec 01 00:00:00 EST 2021

Therapeutic Potential of Mesenchymal Stem Cells in a Pre-Clinical Model of Diabetic Kidney Disease and Obesity.

International journal of molecular sciences

Sávio-Silva C,Soinski-Sousa PE,Simplício-Filho A,Bastos RMC,Beyerstedt S,Rangel ÉB

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2021

Mesenchymal stem cells ameliorate renal fibrosis by galectin-3/Akt/GSK3β/Snail signaling pathway in adenine-induced nephropathy rat.

Stem cell research & therapy

Tang H,Zhang P,Zeng L,Zhao Y,Xie L,Chen B

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

Glutamylation of deubiquitinase BAP1 controls self-renewal of hematopoietic stem cells and hematopoiesis.

The Journal of experimental medicine

Xiong Z,Xia P,Zhu X,Geng J,Wang S,Ye B,Qin X,Qu Y,He L,Fan D,Du Y,Tian Y,Fan Z

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Mon Feb 03 00:00:00 EST 2020

SIRT7 activates quiescent hair follicle stem cells to ensure hair growth in mice.

The EMBO journal

Li G,Tang X,Zhang S,Jin M,Wang M,Deng Z,Liu Z,Qian M,Shi W,Wang Z,Xie H,Li J,Liu B

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunohistochemistry

Tue Sep 15 00:00:00 EDT 2020

A smooth muscle-derived, Braf-driven mouse model of gastrointestinal stromal tumor (GIST): evidence for an alternative GIST cell-of-origin.

The Journal of pathology

Kondo J,Huh WJ,Franklin JL,Heinrich MC,Rubin BP,Coffey RJ

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunocytochemistry

Tue Dec 01 00:00:00 EST 2020

Sema3A and HIF1α co-overexpressed iPSC-MSCs/HA scaffold facilitates the repair of calvarial defect in a mouse model.

Journal of cellular physiology

Li J,Wang T,Li C,Wang Z,Wang P,Zheng L

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Allogeneic adipose-derived stem cells promote ischemic muscle repair by inducing M2 macrophage polarization via the HIF-1α/IL-10 pathway.

Stem cells (Dayton, Ohio)

Liu J,Qiu P,Qin J,Wu X,Wang X,Yang X,Li B,Zhang W,Ye K,Peng Z,Lu X

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Explant culture: A relevant tool for the study of telocytes.

Cell biology international

Sanches BDA,Maldarine JDS,Tamarindo GH,Da Silva ADT,Lima MLD,Rahal P,Góes RM,Taboga SR,Carvalho HF

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunocytochemistry

Tue Dec 01 00:00:00 EST 2020

Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinase.

Nature communications

Wilkes MC,Siva K,Chen J,Varetti G,Youn MY,Chae H,Ek F,Olsson R,Lundbäck T,Dever DP,Nishimura T,Narla A,Glader B,Nakauchi H,Porteus MH,Repellin CE,Gazda HT,Lin S,Serrano M,Flygare J,Sakamoto KM

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Fri Jul 03 00:00:00 EDT 2020

Histone H3 K4/9/27 Trimethylation Levels Affect Wound Healing and Stem Cell Dynamics in Adult Skin.

Stem cell reports

Kang S,Long K,Wang S,Sada A,Tumbar T

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

Small GTPase RAB6 deficiency promotes alveolar progenitor cell renewal and attenuates PM2.5-induced lung injury and fibrosis.

Cell death & disease

Yang L,Liu G,Li X,Xia Z,Wang Y,Lin W,Zhang W,Zhang W,Li X

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Sun Oct 04 00:00:00 EDT 2020

In Silico Analysis of the Age-Dependent Evolution of the Transcriptome of Mouse Skin Stem Cells.

Cells

Lorenzo-Martín LF,Bustelo XR

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Jan 09 00:00:00 EST 2020

Novel CD11b⁺Gr-1⁺Sca-1⁺ myeloid cells drive mortality in bacterial infection.

Science advances

Park MY,Kim HS,Lee HY,Zabel BA,Bae YS

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation.

Nature

Wilkinson AC,Ishida R,Kikuchi M,Sudo K,Morita M,Crisostomo RV,Yamamoto R,Loh KM,Nakamura Y,Watanabe M,Nakauchi H,Yamazaki S

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

Single-nucleotide human disease mutation inactivates a blood-regenerative GATA2 enhancer.

The Journal of clinical investigation

Soukup AA,Zheng Y,Mehta C,Wu J,Liu P,Cao M,Hofmann I,Zhou Y,Zhang J,Johnson KD,Choi K,Keles S,Bresnick EH

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Mesenchymal VEGFA induces aberrant differentiation in heterotopic ossification.

Bone research

Hwang C,Marini S,Huber AK,Stepien DM,Sorkin M,Loder S,Pagani CA,Li J,Visser ND,Vasquez K,Garada MA,Li S,Xu J,Hsu CY,Yu PB,James AW,Mishina Y,Agarwal S,Li J,Levi B

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Sun Oct 17 00:00:00 EDT 2021

Transcriptional Analysis of Sepsis-Induced Activation and Damage of the Adrenal Endothelial Microvascular Cells.

Frontiers in endocrinology

Chen LS,Singh SP,Müller G,Bornstein SR,Kanczkowski W

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunocytochemistry

Fri Feb 14 00:00:00 EST 2020

Dietary calories and lipids synergistically shape adipose tissue cellularity during postnatal growth.

Molecular metabolism

Meln I,Wolff G,Gajek T,Koddebusch J,Lerch S,Harbrecht L,Hong W,Bayindir-Buchhalter I,Krunic D,Augustin HG,Vegiopoulos A

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Tue Mar 05 00:00:00 EST 2019

p110α of PI3K is necessary and sufficient for quiescence exit in adult muscle satellite cells.

The EMBO journal

Wang G,Zhu H,Situ C,Han L,Yu Y,Cheung TH,Liu K,Wu Z

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunocytochemistry

Fri Apr 13 00:00:00 EDT 2018

Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.

Tissue engineering. Part C, Methods

Blackshear CP,Borrelli MR,Shen EZ,Ransom RC,Chung NN,Vistnes SM,Irizarry D,Nazerali R,Momeni A,Longaker MT,Wan DC

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunocytochemistry

Mon Oct 01 00:00:00 EDT 2018

Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation of Cx37, Cx40 and Cx43.

Experimental and therapeutic medicine

Li Z,Zhang S,Cao L,Li W,Ye YC,Shi ZX,Wang ZR,Sun LX,Wang JW,Jia LT,Wang W

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

JunB defines functional and structural integrity of the epidermo-pilosebaceous unit in the skin.

Nature communications

Singh K,Camera E,Krug L,Basu A,Pandey RK,Munir S,Wlaschek M,Kochanek S,Schorpp-Kistner M,Picardo M,Angel P,Niemann C,Maity P,Scharffetter-Kochanek K

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Fri Aug 24 00:00:00 EDT 2018

Age-dependent increase in angiopoietin-like protein 2 accelerates skeletal muscle loss in mice.

The Journal of biological chemistry

Zhao J,Tian Z,Kadomatsu T,Xie P,Miyata K,Sugizaki T,Endo M,Zhu S,Fan H,Horiguchi H,Morinaga J,Terada K,Yoshizawa T,Yamagata K,Oike Y

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunohistochemistry

Fri Feb 02 00:00:00 EST 2018

RhoA controls retinoid signaling by ROCK dependent regulation of retinol metabolism.

Small GTPases

García-Mariscal A,Peyrollier K,Basse A,Pedersen E,Rühl R,van Hengel J,Brakebusch C

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Mon Sep 03 00:00:00 EDT 2018

Proteinase 3 Limits the Number of Hematopoietic Stem and Progenitor Cells in Murine Bone Marrow.

Stem cell reports

Karatepe K,Zhu H,Zhang X,Guo R,Kambara H,Loison F,Liu P,Yu H,Ren Q,Luo X,Manis J,Cheng T,Ma F,Xu Y,Luo HR

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Tue Nov 13 00:00:00 EST 2018

Inflammatory memory sensitizes skin epithelial stem cells to tissue damage.

Nature

Naik S,Larsen SB,Gomez NC,Alaverdyan K,Sendoel A,Yuan S,Polak L,Kulukian A,Chai S,Fuchs E

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Oct 26 00:00:00 EDT 2017

Lactate dehydrogenase activity drives hair follicle stem cell activation.

Nature cell biology

Flores A,Schell J,Krall AS,Jelinek D,Miranda M,Grigorian M,Braas D,White AC,Zhou JL,Graham NA,Graeber T,Seth P,Evseenko D,Coller HA,Rutter J,Christofk HR,Lowry WE

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

14-3-3ζ loss impedes oncogene-induced mammary tumorigenesis and metastasis by attenuating oncogenic signaling.

American journal of cancer research

Joshi S,Yang J,Wang Q,Li P,Wang H,Zhang Q,Xiong Y,Pickering BF,Parker-Thornburg J,Behringer RR,Yu D

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunohistochemistry

Sun Jan 03 00:00:00 EST 2021

A new scaffold containing small intestinal submucosa and mesenchymal stem cells improves pancreatic islet function and survival in vitro and in vivo.

International journal of molecular medicine

Wang D,Ding X,Xue W,Zheng J,Tian X,Li Y,Wang X,Song H,Liu H,Luo X

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

The role of TGFβ‑HGF‑Smad4 axis in regulating the proliferation of mouse airway progenitor cells.

Molecular medicine reports

Li X,Yang L,Sun X,Wu J,Li Y,Zhang Q,Zhang Y,Li K,Wu Q,Chen H

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Tissue-engineered sling with adipose-derived stem cells under static mechanical strain.

Experimental and therapeutic medicine

Wang Y,Wang W,Wang X,Wang Y,Wang J,Fu Q,Shi G

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Zoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB.

PloS one

Gao SY,Zheng GS,Wang L,Liang YJ,Zhang SE,Lao XM,Li K,Liao GQ

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Mon Sep 18 00:00:00 EDT 2017

14-3-3ζ loss leads to neonatal lethality by microRNA-126 downregulation-mediated developmental defects in lung vasculature.

Cell & bioscience

Yang J,Joshi S,Wang Q,Li P,Wang H,Xiong Y,Xiao Y,Wang J,Parker-Thornburg J,Behringer RR,Yu D

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunohistochemistry

Thu Mar 10 00:00:00 EST 2022

Stem cell function and stress response are controlled by protein synthesis.

Nature

Blanco S,Bandiera R,Popis M,Hussain S,Lombard P,Aleksic J,Sajini A,Tanna H,Cortés-Garrido R,Gkatza N,Dietmann S,Frye M

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Jun 16 00:00:00 EDT 2016

Sorting protein VPS33B regulates exosomal autocrine signaling to mediate hematopoiesis and leukemogenesis.

The Journal of clinical investigation

Gu H,Chen C,Hao X,Wang C,Zhang X,Li Z,Shao H,Zeng H,Yu Z,Xie L,Xia F,Zhang F,Liu X,Zhang Y,Jiang H,Zhu J,Wan J,Wang C,Weng W,Xie J,Tao M,Zhang CC,Liu J,Chen GQ,Zheng J

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Serially Transplanted Nonpericytic CD146(-) Adipose Stromal/Stem Cells in Silk Bioscaffolds Regenerate Adipose Tissue In Vivo.

Stem cells (Dayton, Ohio)

Frazier TP,Bowles A,Lee S,Abbott R,Tucker HA,Kaplan D,Wang M,Strong A,Brown Q,He J,Bunnell BA,Gimble JM

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2016

Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification.

Stem cells (Dayton, Ohio)

Agarwal S,Loder SJ,Sorkin M,Li S,Shrestha S,Zhao B,Mishina Y,James AW,Levi B

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Macrophages form functional vascular mimicry channels in vivo.

Scientific reports

Barnett FH,Rosenfeld M,Wood M,Kiosses WB,Usui Y,Marchetti V,Aguilar E,Friedlander M

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Fri Nov 11 00:00:00 EST 2016

Niche-induced cell death and epithelial phagocytosis regulate hair follicle stem cell pool.

Nature

Mesa KR,Rompolas P,Zito G,Myung P,Sun TY,Brown S,Gonzalez DG,Blagoev KB,Haberman AM,Greco V

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Jun 04 00:00:00 EDT 2015

Self-renewing diploid Axin2(+) cells fuel homeostatic renewal of the liver.

Nature

Wang B,Zhao L,Fish M,Logan CY,Nusse R

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Aug 13 00:00:00 EDT 2015

Life-Long Transgene Expression in Skeletal Muscle Without Transduction of Satellite Cells Following Embryonic Myogenic Progenitor Transduction by Lentivirus Administered in Utero.

Stem cells and development

Stitelman DH,Brazelton TR,Endo M,Bora A,Traas J,Zoltick PW,Flake AW

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Sat Aug 15 00:00:00 EDT 2015

Sleep disruption impairs haematopoietic stem cell transplantation in mice.

Nature communications

Rolls A,Pang WW,Ibarra I,Colas D,Bonnavion P,Korin B,Heller HC,Weissman IL,de Lecea L

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Wed Oct 14 00:00:00 EDT 2015

Rejuvenation of the muscle stem cell population restores strength to injured aged muscles.

Nature medicine

Cosgrove BD,Gilbert PM,Porpiglia E,Mourkioti F,Lee SP,Corbel SY,Llewellyn ME,Delp SL,Blau HM

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Sat Mar 01 00:00:00 EST 2014

c-kit+ cells minimally contribute cardiomyocytes to the heart.

Nature

van Berlo JH,Kanisicak O,Maillet M,Vagnozzi RJ,Karch J,Lin SC,Middleton RC,Marbán E,Molkentin JD

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunohistochemistry

Thu May 15 00:00:00 EDT 2014

Mesenchymal-endothelial transition contributes to cardiac neovascularization.

Nature

Ubil E,Duan J,Pillai IC,Rosa-Garrido M,Wu Y,Bargiacchi F,Lu Y,Stanbouly S,Huang J,Rojas M,Vondriska TM,Stefani E,Deb A

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Oct 30 00:00:00 EDT 2014

Atm deletion with dual recombinase technology preferentially radiosensitizes tumor endothelium.

The Journal of clinical investigation

Moding EJ,Lee CL,Castle KD,Oh P,Mao L,Zha S,Min HD,Ma Y,Das S,Kirsch DG

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2014

WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

The Journal of experimental medicine

Xia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Mon Sep 22 00:00:00 EDT 2014

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Dec 11 00:00:00 EST 2014

Myeloid malignancies with chromosome 5q deletions acquire a dependency on an intrachromosomal NF-κB gene network.

Cell reports

Fang J,Barker B,Bolanos L,Liu X,Jerez A,Makishima H,Christie S,Chen X,Rao DS,Grimes HL,Komurov K,Weirauch MT,Cancelas JA,Maciejewski JP,Starczynowski DT

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Sep 11 00:00:00 EDT 2014

Developmental stage determines efficiency of gene transfer to muscle satellite cells by in utero delivery of adeno-associated virus vector serotype 2/9.

Molecular therapy. Methods & clinical development

Stitelman DH,Brazelton T,Bora A,Traas J,Merianos D,Limberis M,Davey M,Flake AW

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Wed May 27 00:00:00 EDT 2015

Usp16 contributes to somatic stem-cell defects in Down's syndrome.

Nature

Adorno M,Sikandar S,Mitra SS,Kuo A,Nicolis Di Robilant B,Haro-Acosta V,Ouadah Y,Quarta M,Rodriguez J,Qian D,Reddy VM,Cheshier S,Garner CC,Clarke MF

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Sep 19 00:00:00 EDT 2013

Sonic hedgehog signals to multiple prostate stromal stem cells that replenish distinct stromal subtypes during regeneration.

Proceedings of the National Academy of Sciences of the United States of America

Peng YC,Levine CM,Zahid S,Wilson EL,Joyner AL

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunohistochemistry (Frozen)

Tue Dec 17 00:00:00 EST 2013

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Microfluidic enrichment of mouse epidermal stem cells and validation of stem cell proliferation in vitro.

Tissue engineering. Part C, Methods

Zhu B,Smith J,Yarmush ML,Nahmias Y,Kirby BJ,Murthy SK

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

Targeted disruption of heparan sulfate interaction with hepatocyte and vascular endothelial growth factors blocks normal and oncogenic signaling.

Cancer cell

Cecchi F,Pajalunga D,Fowler CA,Uren A,Rabe DC,Peruzzi B,Macdonald NJ,Blackman DK,Stahl SJ,Byrd RA,Bottaro DP

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunohistochemistry

Tue Aug 14 00:00:00 EDT 2012

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

Endothelial-derived angiocrine signals induce and sustain regenerative lung alveolarization.

Cell

Ding BS,Nolan DJ,Guo P,Babazadeh AO,Cao Z,Rosenwaks Z,Crystal RG,Simons M,Sato TN,Worgall S,Shido K,Rabbany SY,Rafii S

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Fri Oct 28 00:00:00 EDT 2011

CD34 promotes satellite cell motility and entry into proliferation to facilitate efficient skeletal muscle regeneration.

Stem cells (Dayton, Ohio)

Alfaro LA,Dick SA,Siegel AL,Anonuevo AS,McNagny KM,Megeney LA,Cornelison DD,Rossi FM

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

CD34 promotes satellite cell motility and entry into proliferation to facilitate efficient skeletal muscle regeneration.

Stem cells (Dayton, Ohio)

Alfaro LA,Dick SA,Siegel AL,Anonuevo AS,McNagny KM,Megeney LA,Cornelison DD,Rossi FM

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Improved intratumoral oxygenation through vascular normalization increases glioma sensitivity to ionizing radiation.

International journal of radiation oncology, biology, physics

McGee MC,Hamner JB,Williams RF,Rosati SF,Sims TL,Ng CY,Gaber MW,Calabrese C,Wu J,Nathwani AC,Duntsch C,Merchant TE,Davidoff AM

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunohistochemistry

Thu Apr 01 00:00:00 EDT 2010

Progenitors of skeletal muscle satellite cells express the muscle determination gene, MyoD.

Developmental biology

Kanisicak O,Mendez JJ,Yamamoto S,Yamamoto M,Goldhamer DJ

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunohistochemistry

Sat Aug 01 00:00:00 EDT 2009

Cardiovascular and craniofacial defects in Crk-null mice.

Molecular and cellular biology

Park TJ,Boyd K,Curran T

Published figure using CD34 monoclonal antibody (Product # 53-0341-82) in Immunohistochemistry

Tue Aug 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: CD34; cluster designation 34; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2

View more View less

Gene Aliases: AU040960; Cd34

View more View less

UniProt ID: (Mouse) Q64314

View more View less

Entrez Gene ID: (Mouse) 12490

View more View less

Function(s)

transcription factor binding carbohydrate binding sulfate binding cell adhesion molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD34 Monoclonal Antibody (RAM34), Alexa Fluor™ 488, eBioscience™

CD34 Antibody (53-0341-82) in Flow

Product Details

53-0341-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!