Invitrogen

IFN gamma Monoclonal Antibody (XMG1.2), Alexa Fluor™ 700, eBioscience™

IFN gamma Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 99

IFN gamma Antibody (56-7311-82) in Flow

Mouse splenocytes were stimulated with Concanavalin A (Product # 00-4978) for 2 days then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975) for 5 hours. Cells were then surface stained with Anti-Mouse CD4 FITC (Product # 11-0042), followed by intracellular staining with 0.03 µg of Anti-Mouse IFN gamma Alexa Fluor 700 using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824) and protocol. Cells in the lymphocyte gate were used for analysis.

IFN gamma Antibody in Immunohistochemistry (IHC)

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Product Details

56-7311-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.06 µg/test

View 87 publications 87 publications

ELISA (ELISA)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

XMG1.2

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2688063

Product Specific Information

Description: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN gamma is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.

Applications Reported: This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This XMG1.2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.6 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

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Cell reports

Cham LB,Torrez Dulgeroff LB,Tal MC,Adomati T,Li F,Bhat H,Huang A,Lang PA,Moreno ME,Rivera JM,Galkina SA,Kosikova G,Stoddart CA,McCune JM,Myers LM,Weissman IL,Lang KS,Hasenkrug KJ

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Apr 14 00:00:00 EDT 2020

FUT1 deficiency elicits immune dysregulation and corneal opacity in steady state and under stress.

Cell death & disease

Kim KW,Ryu JS,Ko JH,Kim JY,Kim HJ,Lee HJ,Oh JH,Chung JH,Oh JY

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Fri Apr 24 00:00:00 EDT 2020

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Nov 05 00:00:00 EST 2020

Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse Model.

International journal of molecular sciences

Yoon CH,Ryu JS,Hwang HS,Kim MK

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Jun 11 00:00:00 EDT 2020

BCG vaccination reduces the mortality of Mycobacterium tuberculosis-infected type 2 diabetes mellitus mice.

JCI insight

Radhakrishnan RK,Thandi RS,Tripathi D,Paidipally P,McAllister MK,Mulik S,Samten B,Vankayalapati R

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Mar 12 00:00:00 EDT 2020

Tumors induce de novo steroid biosynthesis in T cells to evade immunity.

Nature communications

Mahata B,Pramanik J,van der Weyden L,Polanski K,Kar G,Riedel A,Chen X,Fonseca NA,Kundu K,Campos LS,Ryder E,Duddy G,Walczak I,Okkenhaug K,Adams DJ,Shields JD,Teichmann SA

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Fri Jul 17 00:00:00 EDT 2020

TAGAP instructs Th17 differentiation by bridging Dectin activation to EPHB2 signaling in innate antifungal response.

Nature communications

Chen J,He R,Sun W,Gao R,Peng Q,Zhu L,Du Y,Ma X,Guo X,Zhang H,Tan C,Wang J,Zhang W,Weng X,Man J,Bauer H,Wang QK,Martin BN,Zhang CJ,Li X,Wang C

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Apr 20 00:00:00 EDT 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

PCC0208025 (BMS202), a small molecule inhibitor of PD-L1, produces an antitumor effect in B16-F10 melanoma-bearing mice.

PloS one

Hu Z,Yu P,Du G,Wang W,Zhu H,Li N,Zhao H,Dong Z,Ye L,Tian J

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Jun 22 00:00:00 EDT 2020

Bmal1 integrates mitochondrial metabolism and macrophage activation.

eLife

Alexander RK,Liou YH,Knudsen NH,Starost KA,Xu C,Hyde AL,Liu S,Jacobi D,Liao NS,Lee CH

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue May 12 00:00:00 EDT 2020

PPARα Inhibition Overcomes Tumor-Derived Exosomal Lipid-Induced Dendritic Cell Dysfunction.

Cell reports

Yin X,Zeng W,Wu B,Wang L,Wang Z,Tian H,Wang L,Jiang Y,Clay R,Wei X,Qin Y,Zhang F,Zhang C,Jin L,Liang W

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in ELISA

Tue Oct 20 00:00:00 EDT 2020

Tolerogenic Immunomodulation by PEGylated Antigenic Peptides.

Frontiers in immunology

Pfeil J,Simonetti M,Lauer U,Volkmer R,von Thülen B,Durek P,Krähmer R,Leenders F,Hamann A,Hoffmann U

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Apr 29 00:00:00 EDT 2021

Bixin Attenuates Experimental Autoimmune Encephalomyelitis by Suppressing TXNIP/NLRP3 Inflammasome Activity and Activating NRF2 Signaling.

Frontiers in immunology

Yu Y,Wu DM,Li J,Deng SH,Liu T,Zhang T,He M,Zhao YY,Xu Y

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Jun 21 00:00:00 EDT 2021

Specific targeting of CD163⁺ TAMs mobilizes inflammatory monocytes and promotes T cell-mediated tumor regression.

The Journal of experimental medicine

Etzerodt A,Tsalkitzi K,Maniecki M,Damsky W,Delfini M,Baudoin E,Moulin M,Bosenberg M,Graversen JH,Auphan-Anezin N,Moestrup SK,Lawrence T

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Oct 07 00:00:00 EDT 2019

BTK signaling drives CD1d^hiCD5⁺ regulatory B-cell differentiation to promote pancreatic carcinogenesis.

Oncogene

Das S,Bar-Sagi D

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

The ROP16III-dependent early immune response determines the subacute CNS immune response and type III Toxoplasma gondii survival.

PLoS pathogens

Tuladhar S,Kochanowsky JA,Bhaskara A,Ghotmi Y,Chandrasekaran S,Koshy AA

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

CD8⁺ T cells promote cytokine responses to stress.

Cytokine

Clark SM,Song C,Li X,Keegan AD,Tonelli LH

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

IL-31 plays dual roles in lung inflammation in an OVA-induced murine asthma model.

Biology open

Huang J,Yue H,Jiang T,Gao J,Shi Y,Shi B,Wu X,Gou X

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Jan 15 00:00:00 EST 2019

An attenuated Zika virus NS4B protein mutant is a potent inducer of antiviral immune responses.

NPJ vaccines

Li G,Adam A,Luo H,Shan C,Cao Z,Fontes-Garfias CR,Sarathy VV,Teleki C,Winkelmann ER,Liang Y,Sun J,Bourne N,Barrett ADT,Shi PY,Wang T

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression.

Nature communications

Cao Y,Trillo-Tinoco J,Sierra RA,Anadon C,Dai W,Mohamed E,Cen L,Costich TL,Magliocco A,Marchion D,Klar R,Michel S,Jaschinski F,Reich RR,Mehrotra S,Cubillos-Ruiz JR,Munn DH,Conejo-Garcia JR,Rodriguez PC

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Wed Mar 20 00:00:00 EDT 2019

Innate-Like Lymphocytes Are Immediate Participants in the Hyper-Acute Immune Response to Trauma and Hemorrhagic Shock.

Frontiers in immunology

Manson J,Hoffman R,Chen S,Ramadan MH,Billiar TR

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Oct 13 00:00:00 EDT 2020

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Jun 28 00:00:00 EDT 2018

Hippo Kinases Mst1 and Mst2 Sense and Amplify IL-2R-STAT5 Signaling in Regulatory T Cells to Establish Stable Regulatory Activity.

Immunity

Shi H,Liu C,Tan H,Li Y,Nguyen TM,Dhungana Y,Guy C,Vogel P,Neale G,Rankin S,Feng Y,Peng J,Tao W,Chi H

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

SYK-CARD9 Signaling Axis Promotes Gut Fungi-Mediated Inflammasome Activation to Restrict Colitis and Colon Cancer.

Immunity

Malik A,Sharma D,Malireddi RKS,Guy CS,Chang TC,Olsen SR,Neale G,Vogel P,Kanneganti TD

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Sep 18 00:00:00 EDT 2018

Critical Role for the Microbiota in CX₃CR1⁺ Intestinal Mononuclear Phagocyte Regulation of Intestinal T Cell Responses.

Immunity

Kim M,Galan C,Hill AA,Wu WJ,Fehlner-Peach H,Song HW,Schady D,Bettini ML,Simpson KW,Longman RS,Littman DR,Diehl GE

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in ELISA

Tue Jul 17 00:00:00 EDT 2018

MicroRNA-126 deficiency enhanced the activation and function of CD4⁺ T cells by elevating IRS-1 pathway.

Clinical and experimental immunology

Chu F,Hu Y,Zhou Y,Guo M,Lu J,Zheng W,Xu H,Zhao J,Xu L

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

mTOR Modulates CD8+ T Cell Differentiation in Mice with Invasive Pulmonary Aspergillosis.

Open life sciences

Wang H,Xiao Y,Su L,Cui N,Liu D

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Microbial, metabolomic, and immunologic dynamics in a relapsing genetic mouse model of colitis induced by T-synthase deficiency.

Gut microbes

Jacobs JP,Lin L,Goudarzi M,Ruegger P,McGovern DP,Fornace AJ,Borneman J,Xia L,Braun J

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Jan 02 00:00:00 EST 2017

NLRC3 is an inhibitory sensor of PI3K-mTOR pathways in cancer.

Nature

Karki R,Man SM,Malireddi RKS,Kesavardhana S,Zhu Q,Burton AR,Sharma BR,Qi X,Pelletier S,Vogel P,Rosenstiel P,Kanneganti TD

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Dec 22 00:00:00 EST 2016

MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice.

Proceedings of the National Academy of Sciences of the United States of America

Wu L,Li N,Zhang M,Xue SL,Cassady K,Lin Q,Riggs AD,Zeng D

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Dec 29 00:00:00 EST 2015

Enhanced T-cell activation and differentiation in lymphocytes from transgenic mice expressing ubiquitination-resistant 2KR LAT molecules.

Gene therapy

Rodriguez-Peña AB,Gomez-Rodriguez J,Kortum RL,Palmer DC,Yu Z,Guittard GC,Wohlfert EA,Silver PB,Misplon JA,Sommers CL,Feigenbaum L,Epstein SL,Caspi RR,Belkaid Y,Restifo NP,Samelson LE,Balagopalan L

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

The Nuclear Orphan Receptor NR2F6 Is a Central Checkpoint for Cancer Immune Surveillance.

Cell reports

Hermann-Kleiter N,Klepsch V,Wallner S,Siegmund K,Klepsch S,Tuzlak S,Villunger A,Kaminski S,Pfeifhofer-Obermair C,Gruber T,Wolf D,Baier G

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2015

Treatment with targeted vesicular stomatitis virus generates therapeutic multifunctional anti-tumor memory CD4 T cells.

Cancer gene therapy

Gao Y,Whitaker-Dowling P,Griffin JA,Bergman I

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Inhibition of SOCS1-/- lethal autoinflammatory disease correlated to enhanced peripheral Foxp3+ regulatory T cell homeostasis.

Journal of immunology (Baltimore, Md. : 1950)

Collins EL,Jager LD,Dabelic R,Benitez P,Holdstein K,Lau K,Haider MI,Johnson HM,Larkin J

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in ELISA

Thu Sep 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

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Gene Aliases: Ifg; IFN-g; Ifng

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UniProt ID: (Mouse) P01580

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Entrez Gene ID: (Mouse) 15978

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Function(s)

cytokine activity interferon-gamma receptor binding protein binding cytokine interferon superfamily intercellular signal molecule

Process(es)

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IFN gamma Monoclonal Antibody (XMG1.2), Alexa Fluor™ 700, eBioscience™

IFN gamma Antibody (56-7311-82) in Flow

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56-7311-82

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