Invitrogen

IFN gamma Monoclonal Antibody (XMG1.2), Alexa Fluor™ 700, eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (98)

IFN gamma Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 99

IFN gamma Antibody (56-7311-82) in Flow

Mouse splenocytes were stimulated with Concanavalin A (Product # 00-4978) for 2 days then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975) for 5 hours. Cells were then surface stained with Anti-Mouse CD4 FITC (Product # 11-0042), followed by intracellular staining with 0.03 µg of Anti-Mouse IFN gamma Alexa Fluor 700 using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824) and protocol. Cells in the lymphocyte gate were used for analysis.

IFN gamma Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

56-7311-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.06 µg/test

View 84 publications 84 publications

ELISA (ELISA)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

XMG1.2

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2688063

Product Specific Information

Description: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN gamma is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.

Applications Reported: This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This XMG1.2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.6 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

T cell-derived interleukin-22 drives the expression of CD155 by cancer cells to suppress NK cell function and promote metastasis.

Immunity

Briukhovetska D,Suarez-Gosalvez J,Voigt C,Markota A,Giannou AD,Schübel M,Jobst J,Zhang T,Dörr J,Märkl F,Majed L,Müller PJ,May P,Gottschlich A,Tokarew N,Lücke J,Oner A,Schwerdtfeger M,Andreu-Sanz D,Grünmeier R,Seifert M,Michaelides S,Hristov M,König LM,Cadilha BL,Mikhaylov O,Anders HJ,Rothenfusser S,Flavell RA,Cerezo-Wallis D,Tejedo C,Soengas MS,Bald T,Huber S,Endres S,Kobold S

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Jan 10 00:00:00 EST 2023

Biometabolites of Citrus unshiu Peel Enhance Intestinal Permeability and Alter Gut Commensal Bacteria.

Nutrients

Lee SH,Seo D,Lee KH,Park SJ,Park S,Kim H,Kim T,Joo IH,Park JM,Kang YH,Lim GH,Kim DH,Yang JY

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Jan 09 00:00:00 EST 2023

Alleviating psoriatic skin inflammation through augmentation of Treg cells via CTLA-4 signaling peptide.

Frontiers in immunology

Lee WS,Nam KH,Kim JH,Kim WJ,Kim JE,Shin EC,Kim GR,Choi JM

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11-Mediated IL18 in the Gut Epithelial Cells.

Cellular and molecular gastroenterology and hepatology

Zhang Q,Su X,Zhang C,Chen W,Wang Y,Yang X,Liu D,Zhang Y,Yang R

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Feb 23 00:00:00 EST 2023

Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challenge.

Biomaterials

Sun S,Li E,Zhao G,Tang J,Zuo Q,Cai L,Xu C,Sui C,Ou Y,Liu C,Li H,Ding Y,Li C,Lu D,Zhang W,Luo P,Cheng P,Gao Y,Tu C,Pitard B,Rosenecker J,Wang B,Liu Y,Zou Q,Guan S

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2023

Histone methyltransferase WHSC1 loss dampens MHC-I antigen presentation pathway to impair IFN-γ-stimulated antitumor immunity.

The Journal of clinical investigation

Ren J,Li N,Pei S,Lian Y,Li L,Peng Y,Liu Q,Guo J,Wang X,Han Y,Zhang G,Wang H,Li Y,Jiang J,Li Q,Tan M,Peng J,Hu G,Xiao Y,Li X,Lin M,Qin J

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2022

A peptide encoded by pri-miRNA-31 represses autoimmunity by promoting T_reg differentiation.

EMBO reports

Zhou H,Lou F,Bai J,Sun Y,Cai W,Sun L,Xu Z,Liu Z,Zhang L,Yin Q,Zhang J,Gao Y,Wang Z,Niu L,Cai X,Deng S,Wang H,Xia L,Ginhoux F,Li Q,Wang H

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Wed May 04 00:00:00 EDT 2022

Penfluridol targets acid sphingomyelinase to inhibit TNF signaling and is therapeutic against inflammatory autoimmune diseases.

Arthritis research & therapy

Chen YH,Liu RH,Cui YZ,Hettinghouse A,Fu WY,Zhang L,Zhang C,Liu CJ

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Wed Jan 19 00:00:00 EST 2022

Neutrophil extracellular traps and their histones promote Th17 cell differentiation directly via TLR2.

Nature communications

Wilson AS,Randall KL,Pettitt JA,Ellyard JI,Blumenthal A,Enders A,Quah BJ,Bopp T,Parish CR,Brüstle A

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Wed Jan 26 00:00:00 EST 2022

Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus Infection.

International journal of molecular sciences

Yang G,Wang J,Wang S,Zhu Y

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Sat Aug 20 00:00:00 EDT 2022

Dendritic cell Piezo1 directs the differentiation of T_H1 and T_reg cells in cancer.

eLife

Wang Y,Yang H,Jia A,Wang Y,Yang Q,Dong Y,Hou Y,Cao Y,Dong L,Bi Y,Liu G

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Aug 22 00:00:00 EDT 2022

Leishmania donovani Impedes Antileishmanial Immunity by Suppressing Dendritic Cells via the TIM-3 Receptor.

mBio

Akhtar MN,Kumar S,Sen P

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Aug 30 00:00:00 EDT 2022

Agr2-associated ER stress promotes adherent-invasive E. coli dysbiosis and triggers CD103⁺ dendritic cell IL-23-dependent ileocolitis.

Cell reports

Viladomiu M,Khounlotham M,Dogan B,Lima SF,Elsaadi A,Cardakli E,Castellanos JG,Ng C,Herzog J,Schoenborn AA,Ellermann M,Liu B,Zhang S,Gulati AS,Sartor RB,Simpson KW,Lipkin SM,Longman RS

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2022

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression.

Nature communications

Qi M,Xia Y,Wu Y,Zhang Z,Wang X,Lu L,Dai C,Song Y,Xu K,Ji W,Zhan L

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Wed Feb 16 00:00:00 EST 2022

Breast cancer cell-derived extracellular vesicles promote CD8⁺ T cell exhaustion via TGF-β type II receptor signaling.

Nature communications

Xie F,Zhou X,Su P,Li H,Tu Y,Du J,Pan C,Wei X,Zheng M,Jin K,Miao L,Wang C,Meng X,van Dam H,Ten Dijke P,Zhang L,Zhou F

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Local and Systemic Injections of Human Cord Blood Myeloid-Derived Suppressor Cells to Prevent Graft Rejection in Corneal Transplantation.

Biomedicines

Lee JY,Sohn HJ,Kim CH,Kim TG,Lee HS

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Dec 12 00:00:00 EST 2022

Transcription factor RORα enforces stability of the Th17 cell effector program by binding to a Rorc cis-regulatory element.

Immunity

Hall JA,Pokrovskii M,Kroehling L,Kim BR,Kim SY,Wu L,Lee JY,Littman DR

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Nov 08 00:00:00 EST 2022

Lactate induces metabolic and epigenetic reprogramming of pro-inflammatory Th17 cells.

EMBO reports

Lopez Krol A,Nehring HP,Krause FF,Wempe A,Raifer H,Nist A,Stiewe T,Bertrams W,Schmeck B,Luu M,Leister H,Chung HR,Bauer UM,Adhikary T,Visekruna A

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Dec 06 00:00:00 EST 2022

Endogenous retroviruses promote homeostatic and inflammatory responses to the microbiota.

Cell

Lima-Junior DS,Krishnamurthy SR,Bouladoux N,Collins N,Han SJ,Chen EY,Constantinides MG,Link VM,Lim AI,Enamorado M,Cataisson C,Gil L,Rao I,Farley TK,Koroleva G,Attig J,Yuspa SH,Fischbach MA,Kassiotis G,Belkaid Y

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Jul 08 00:00:00 EDT 2021

The CD94/NKG2A inhibitory receptor educates uterine NK cells to optimize pregnancy outcomes in humans and mice.

Immunity

Shreeve N,Depierreux D,Hawkes D,Traherne JA,Sovio U,Huhn O,Jayaraman J,Horowitz A,Ghadially H,Perry JRB,Moffett A,Sled JG,Sharkey AM,Colucci F

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13.

Cellular & molecular immunology

Mulas F,Wang X,Song S,Nishanth G,Yi W,Brunn A,Larsen PK,Isermann B,Kalinke U,Barragan A,Naumann M,Deckert M,Schlüter D

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

AIM2 controls microglial inflammation to prevent experimental autoimmune encephalomyelitis.

The Journal of experimental medicine

Ma C,Li S,Hu Y,Ma Y,Wu Y,Wu C,Liu X,Wang B,Hu G,Zhou J,Yang S

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon May 03 00:00:00 EDT 2021

Mapping and role of T cell response in SARS-CoV-2-infected mice.

The Journal of experimental medicine

Zhuang Z,Lai X,Sun J,Chen Z,Zhang Z,Dai J,Liu D,Li Y,Li F,Wang Y,Zhu A,Wang J,Yang W,Huang J,Li X,Hu L,Wen L,Zhuo J,Zhang Y,Chen D,Li S,Huang S,Shi Y,Zheng K,Zhong N,Zhao J,Zhou D,Zhao J

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Apr 05 00:00:00 EDT 2021

Low immunogenicity of malaria pre-erythrocytic stages can be overcome by vaccination.

EMBO molecular medicine

Müller K,Gibbins MP,Roberts M,Reyes-Sandoval A,Hill AVS,Draper SJ,Matuschewski K,Silvie O,Hafalla JCR

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Fri Apr 09 00:00:00 EDT 2021

High Ambient Temperature Aggravates Experimental Autoimmune Uveitis Symptoms.

Frontiers in cell and developmental biology

Pan S,Tan H,Chang R,Wang Q,Zhu Y,Chen L,Li H,Su G,Zhou C,Cao Q,Kijlstra A,Yang P

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Senescent T Cell Induces Brown Adipose Tissue "Whitening" Via Secreting IFN-γ.

Frontiers in cell and developmental biology

Pan XX,Yao KL,Yang YF,Ge Q,Zhang R,Gao PJ,Ruan CC,Wu F

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Mar 23 00:00:00 EDT 2021

Trichinella spiralis Paramyosin Induces Colonic Regulatory T Cells to Mitigate Inflammatory Bowel Disease.

Frontiers in cell and developmental biology

Hao C,Wang W,Zhan B,Wang Z,Huang J,Sun X,Zhu X

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Aug 03 00:00:00 EDT 2021

Prevention of EAE by tolerogenic vaccination with PEGylated antigenic peptides.

Therapeutic advances in chronic disease

Pfeil J,Simonetti M,Lauer U,von Thülen B,Durek P,Poulsen C,Pawlowska J,Kröger M,Krähmer R,Leenders F,Hoffmann U,Hamann A

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Nov 07 00:00:00 EST 2023

Generation of a Novel Oncolytic Vaccinia Virus Using the IHD-W Strain.

Human gene therapy

Shin J,Hong SO,Kim M,Lee H,Choi H,Kim J,Hong J,Kang H,Lee E,Lee S,Kong B,Kim M,Choi H,Kim S

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Identification of CTL Epitopes on Efflux Pumps of the ATP-Binding Cassette and the Major Facilitator Superfamily of Mycobacterium tuberculosis.

Journal of immunology research

Lin Y,Dong Y,Gao Y,Shi R,Li Y,Zhou X,Liu W,Li G,Qi Y,Wu Y

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Sep 20 00:00:00 EDT 2021

Fine particulate matter induces airway inflammation by disturbing the balance between Th1/Th2 and regulation of GATA3 and Runx3 expression in BALB/c mice.

Molecular medicine reports

Pang L,Yu P,Liu X,Fan Y,Shi Y,Zou S

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells.

Oncology letters

Chen J,Ding Y,Huang F,Lan R,Wang Z,Huang W,Chen R,Wu B,Fu L,Yang Y,Liu J,Hong J,Zhang W,Zhang L

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Features of T-cell subset composition in a D-galactose-induced senescence mouse model.

Experimental animals

Kawata K,Suzuki T,Ozawa K,Sekiguchi M

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Fri Aug 06 00:00:00 EDT 2021

Knockout of the KH-Type Splicing Regulatory Protein Drives Glomerulonephritis in MRL-Fas^lpr Mice.

Cells

Schmidtke L,Meineck M,Saurin S,Otten S,Gather F,Schrick K,Käfer R,Roth W,Kleinert H,Weinmann-Menke J,Pautz A

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Immunohistochemistry

Sun Nov 14 00:00:00 EST 2021

T cell-derived tumor necrosis factor induces cytotoxicity by activating RIPK1-dependent target cell death.

JCI insight

Chun N,Ang RL,Chan M,Fairchild RL,Baldwin WM,Horwitz JK,Gelles JD,Chipuk JE,Kelliher MA,Pavlov VI,Li Y,Homann D,Heeger PS,Ting AT

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Wed Dec 22 00:00:00 EST 2021

Antitumor efficacy of a neoantigen cancer vaccine delivered by electroporation is influenced by microbiota composition.

Oncoimmunology

Lione L,Salvatori E,Petrazzuolo A,Massacci A,Maggio R,Confroti A,Compagnone M,Aurisicchio L,Ciliberto G,Palombo F

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Mar 23 00:00:00 EDT 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Expansion of an Unusual Virtual Memory CD8⁺ Subpopulation Bearing Vα3.2 TCR in Themis-Deficient Mice.

Frontiers in immunology

Prasad M,Wojciech L,Brzostek J,Hu J,Chua YL,Tung DWH,Yap J,Rybakin V,Gascoigne NRJ

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Sep 23 00:00:00 EDT 2021

Type 1 Cytotoxic T Cells Increase in Placenta after Intrauterine Inflammation.

Frontiers in immunology

Liu J,Liu Y,Panda S,Liu A,Lei J,Burd I

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Macrophages Compensate for Loss of Protein Tyrosine Phosphatase N₂ in Dendritic Cells to Protect from Elevated Colitis.

International journal of molecular sciences

Hering L,Katkeviciute E,Schwarzfischer M,Niechcial A,Riggs JB,Wawrzyniak M,Atrott K,van de Sande M,Lang S,Becher B,Rogler G,Scharl M,Spalinger MR

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Immunohistochemistry

Fri Jun 25 00:00:00 EDT 2021

Exosome-mediated mRNA delivery in vivo is safe and can be used to induce SARS-CoV-2 immunity.

The Journal of biological chemistry

Tsai SJ,Atai NA,Cacciottolo M,Nice J,Salehi A,Guo C,Sedgwick A,Kanagavelu S,Gould SJ

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer.

Nature communications

Huang Y,Guan Z,Dai X,Shen Y,Wei Q,Ren L,Jiang J,Xiao Z,Jiang Y,Liu D,Huang Z,Xu X,Luo Y,Zhao C

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Mechanisms that allow vaccination against an oncolytic vesicular stomatitis virus-encoded transgene to enhance safety without abrogating oncolysis.

Scientific reports

AuYeung AWK,Mould RC,Stegelmeier AA,van Vloten JP,Karimi K,Woods JP,Petrik JJ,Wood GA,Bridle BW

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

YY1 regulation by miR-124-3p promotes Th17 cell pathogenicity through interaction with T-bet in rheumatoid arthritis.

JCI insight

Lin J,Tang J,Lin J,He Y,Yu Z,Jiang R,Yang B,Ou Q

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Nov 22 00:00:00 EST 2021

Small Molecule Inhibitors Targeting Nuclear Factor κB Activation Markedly Reduce Expression of Interleukin-2, but Not Interferon-γ, Induced by Phorbol Esters and Calcium Ionophores.

International journal of molecular sciences

Tanaka Y,Nakao A,Miyake Y,Higashi Y,Tanigaki R,Kataoka T

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Fri Dec 03 00:00:00 EST 2021

SPARC regulation of PMN clearance protects from pristane-induced lupus and rheumatoid arthritis.

iScience

Sangaletti S,Botti L,Gulino A,Lecis D,Bassani B,Portararo P,Milani M,Cancila V,De Cecco L,Dugo M,Tripodo C,Colombo MP

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

Non-redundant activity of GSK-3α and GSK-3β in T cell-mediated tumor rejection.

iScience

Steele L,Mannion AJ,Shaw G,Maclennan KA,Cook GP,Rudd CE,Taylor A

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry.

STAR protocols

Skordos I,Demeyer A,Beyaert R

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade.

Immunity

Bonavita E,Bromley CP,Jonsson G,Pelly VS,Sahoo S,Walwyn-Brown K,Mensurado S,Moeini A,Flanagan E,Bell CR,Chiang SC,Chikkanna-Gowda CP,Rogers N,Silva-Santos B,Jaillon S,Mantovani A,Reis e Sousa C,Guerra N,Davis DM,Zelenay S

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2020

PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2.

The Journal of cell biology

Xu X,Hou B,Fulzele A,Masubuchi T,Zhao Y,Wu Z,Hu Y,Jiang Y,Ma Y,Wang H,Bennett EJ,Fu G,Hui E

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Runx proteins mediate protective immunity against Leishmania donovani infection by promoting CD40 expression on dendritic cells.

PLoS pathogens

Akhtar MN,Mishra M,Yadav V,Yadav M,Gujar R,Lal S,Kumar R,Khatri N,Sen P

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Isolation and functional characterisation of lamina propria leukocytes from helminth-infected, murine small intestine.

Journal of immunological methods

Webster HC,Andrusaite AT,Shergold AL,Milling SWF,Perona-Wright G

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Lysosomal Acid Lipase Is Required for Donor T Cells to Induce Graft-versus-Host Disease.

Cell reports

Nguyen HD,Ticer T,Bastian D,Kuril S,Li H,Du H,Yan C,Yu XZ

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Oct 27 00:00:00 EDT 2020

C5aR1 Activation Drives Early IFN-γ Production to Control Experimental Toxoplasma gondii Infection.

Frontiers in immunology

Briukhovetska D,Ohm B,Mey FT,Aliberti J,Kleingarn M,Huber-Lang M,Karsten CM,Köhl J

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Apr 05 00:00:00 EDT 2021

Immunotherapeutic Blockade of CD47 Inhibitory Signaling Enhances Innate and Adaptive Immune Responses to Viral Infection.

Cell reports

Cham LB,Torrez Dulgeroff LB,Tal MC,Adomati T,Li F,Bhat H,Huang A,Lang PA,Moreno ME,Rivera JM,Galkina SA,Kosikova G,Stoddart CA,McCune JM,Myers LM,Weissman IL,Lang KS,Hasenkrug KJ

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Apr 14 00:00:00 EDT 2020

FUT1 deficiency elicits immune dysregulation and corneal opacity in steady state and under stress.

Cell death & disease

Kim KW,Ryu JS,Ko JH,Kim JY,Kim HJ,Lee HJ,Oh JH,Chung JH,Oh JY

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Fri Apr 24 00:00:00 EDT 2020

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Nov 05 00:00:00 EST 2020

Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse Model.

International journal of molecular sciences

Yoon CH,Ryu JS,Hwang HS,Kim MK

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Jun 11 00:00:00 EDT 2020

BCG vaccination reduces the mortality of Mycobacterium tuberculosis-infected type 2 diabetes mellitus mice.

JCI insight

Radhakrishnan RK,Thandi RS,Tripathi D,Paidipally P,McAllister MK,Mulik S,Samten B,Vankayalapati R

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Mar 12 00:00:00 EDT 2020

Tumors induce de novo steroid biosynthesis in T cells to evade immunity.

Nature communications

Mahata B,Pramanik J,van der Weyden L,Polanski K,Kar G,Riedel A,Chen X,Fonseca NA,Kundu K,Campos LS,Ryder E,Duddy G,Walczak I,Okkenhaug K,Adams DJ,Shields JD,Teichmann SA

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Fri Jul 17 00:00:00 EDT 2020

TAGAP instructs Th17 differentiation by bridging Dectin activation to EPHB2 signaling in innate antifungal response.

Nature communications

Chen J,He R,Sun W,Gao R,Peng Q,Zhu L,Du Y,Ma X,Guo X,Zhang H,Tan C,Wang J,Zhang W,Weng X,Man J,Bauer H,Wang QK,Martin BN,Zhang CJ,Li X,Wang C

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Apr 20 00:00:00 EDT 2020

PPARα Inhibition Overcomes Tumor-Derived Exosomal Lipid-Induced Dendritic Cell Dysfunction.

Cell reports

Yin X,Zeng W,Wu B,Wang L,Wang Z,Tian H,Wang L,Jiang Y,Clay R,Wei X,Qin Y,Zhang F,Zhang C,Jin L,Liang W

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in ELISA

Tue Oct 20 00:00:00 EDT 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

PCC0208025 (BMS202), a small molecule inhibitor of PD-L1, produces an antitumor effect in B16-F10 melanoma-bearing mice.

PloS one

Hu Z,Yu P,Du G,Wang W,Zhu H,Li N,Zhao H,Dong Z,Ye L,Tian J

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Jun 22 00:00:00 EDT 2020

Bmal1 integrates mitochondrial metabolism and macrophage activation.

eLife

Alexander RK,Liou YH,Knudsen NH,Starost KA,Xu C,Hyde AL,Liu S,Jacobi D,Liao NS,Lee CH

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue May 12 00:00:00 EDT 2020

Tolerogenic Immunomodulation by PEGylated Antigenic Peptides.

Frontiers in immunology

Pfeil J,Simonetti M,Lauer U,Volkmer R,von Thülen B,Durek P,Krähmer R,Leenders F,Hamann A,Hoffmann U

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Apr 29 00:00:00 EDT 2021

Bixin Attenuates Experimental Autoimmune Encephalomyelitis by Suppressing TXNIP/NLRP3 Inflammasome Activity and Activating NRF2 Signaling.

Frontiers in immunology

Yu Y,Wu DM,Li J,Deng SH,Liu T,Zhang T,He M,Zhao YY,Xu Y

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Jun 21 00:00:00 EDT 2021

Specific targeting of CD163⁺ TAMs mobilizes inflammatory monocytes and promotes T cell-mediated tumor regression.

The Journal of experimental medicine

Etzerodt A,Tsalkitzi K,Maniecki M,Damsky W,Delfini M,Baudoin E,Moulin M,Bosenberg M,Graversen JH,Auphan-Anezin N,Moestrup SK,Lawrence T

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Oct 07 00:00:00 EDT 2019

BTK signaling drives CD1d^hiCD5⁺ regulatory B-cell differentiation to promote pancreatic carcinogenesis.

Oncogene

Das S,Bar-Sagi D

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

The ROP16III-dependent early immune response determines the subacute CNS immune response and type III Toxoplasma gondii survival.

PLoS pathogens

Tuladhar S,Kochanowsky JA,Bhaskara A,Ghotmi Y,Chandrasekaran S,Koshy AA

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

CD8⁺ T cells promote cytokine responses to stress.

Cytokine

Clark SM,Song C,Li X,Keegan AD,Tonelli LH

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

IL-31 plays dual roles in lung inflammation in an OVA-induced murine asthma model.

Biology open

Huang J,Yue H,Jiang T,Gao J,Shi Y,Shi B,Wu X,Gou X

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Jan 15 00:00:00 EST 2019

An attenuated Zika virus NS4B protein mutant is a potent inducer of antiviral immune responses.

NPJ vaccines

Li G,Adam A,Luo H,Shan C,Cao Z,Fontes-Garfias CR,Sarathy VV,Teleki C,Winkelmann ER,Liang Y,Sun J,Bourne N,Barrett ADT,Shi PY,Wang T

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression.

Nature communications

Cao Y,Trillo-Tinoco J,Sierra RA,Anadon C,Dai W,Mohamed E,Cen L,Costich TL,Magliocco A,Marchion D,Klar R,Michel S,Jaschinski F,Reich RR,Mehrotra S,Cubillos-Ruiz JR,Munn DH,Conejo-Garcia JR,Rodriguez PC

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Wed Mar 20 00:00:00 EDT 2019

Innate-Like Lymphocytes Are Immediate Participants in the Hyper-Acute Immune Response to Trauma and Hemorrhagic Shock.

Frontiers in immunology

Manson J,Hoffman R,Chen S,Ramadan MH,Billiar TR

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Oct 13 00:00:00 EDT 2020

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Jun 28 00:00:00 EDT 2018

Hippo Kinases Mst1 and Mst2 Sense and Amplify IL-2R-STAT5 Signaling in Regulatory T Cells to Establish Stable Regulatory Activity.

Immunity

Shi H,Liu C,Tan H,Li Y,Nguyen TM,Dhungana Y,Guy C,Vogel P,Neale G,Rankin S,Feng Y,Peng J,Tao W,Chi H

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

SYK-CARD9 Signaling Axis Promotes Gut Fungi-Mediated Inflammasome Activation to Restrict Colitis and Colon Cancer.

Immunity

Malik A,Sharma D,Malireddi RKS,Guy CS,Chang TC,Olsen SR,Neale G,Vogel P,Kanneganti TD

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Sep 18 00:00:00 EDT 2018

Critical Role for the Microbiota in CX₃CR1⁺ Intestinal Mononuclear Phagocyte Regulation of Intestinal T Cell Responses.

Immunity

Kim M,Galan C,Hill AA,Wu WJ,Fehlner-Peach H,Song HW,Schady D,Bettini ML,Simpson KW,Longman RS,Littman DR,Diehl GE

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in ELISA

Tue Jul 17 00:00:00 EDT 2018

MicroRNA-126 deficiency enhanced the activation and function of CD4⁺ T cells by elevating IRS-1 pathway.

Clinical and experimental immunology

Chu F,Hu Y,Zhou Y,Guo M,Lu J,Zheng W,Xu H,Zhao J,Xu L

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

mTOR Modulates CD8+ T Cell Differentiation in Mice with Invasive Pulmonary Aspergillosis.

Open life sciences

Wang H,Xiao Y,Su L,Cui N,Liu D

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Microbial, metabolomic, and immunologic dynamics in a relapsing genetic mouse model of colitis induced by T-synthase deficiency.

Gut microbes

Jacobs JP,Lin L,Goudarzi M,Ruegger P,McGovern DP,Fornace AJ,Borneman J,Xia L,Braun J

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Mon Jan 02 00:00:00 EST 2017

NLRC3 is an inhibitory sensor of PI3K-mTOR pathways in cancer.

Nature

Karki R,Man SM,Malireddi RKS,Kesavardhana S,Zhu Q,Burton AR,Sharma BR,Qi X,Pelletier S,Vogel P,Rosenstiel P,Kanneganti TD

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Dec 22 00:00:00 EST 2016

MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice.

Proceedings of the National Academy of Sciences of the United States of America

Wu L,Li N,Zhang M,Xue SL,Cassady K,Lin Q,Riggs AD,Zeng D

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Dec 29 00:00:00 EST 2015

Enhanced T-cell activation and differentiation in lymphocytes from transgenic mice expressing ubiquitination-resistant 2KR LAT molecules.

Gene therapy

Rodriguez-Peña AB,Gomez-Rodriguez J,Kortum RL,Palmer DC,Yu Z,Guittard GC,Wohlfert EA,Silver PB,Misplon JA,Sommers CL,Feigenbaum L,Epstein SL,Caspi RR,Belkaid Y,Restifo NP,Samelson LE,Balagopalan L

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

The Nuclear Orphan Receptor NR2F6 Is a Central Checkpoint for Cancer Immune Surveillance.

Cell reports

Hermann-Kleiter N,Klepsch V,Wallner S,Siegmund K,Klepsch S,Tuzlak S,Villunger A,Kaminski S,Pfeifhofer-Obermair C,Gruber T,Wolf D,Baier G

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2015

Treatment with targeted vesicular stomatitis virus generates therapeutic multifunctional anti-tumor memory CD4 T cells.

Cancer gene therapy

Gao Y,Whitaker-Dowling P,Griffin JA,Bergman I

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Inhibition of SOCS1-/- lethal autoinflammatory disease correlated to enhanced peripheral Foxp3+ regulatory T cell homeostasis.

Journal of immunology (Baltimore, Md. : 1950)

Collins EL,Jager LD,Dabelic R,Benitez P,Holdstein K,Lau K,Haider MI,Johnson HM,Larkin J

Published figure using IFN gamma monoclonal antibody (Product # 56-7311-82) in ELISA

Thu Sep 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

View more View less

Gene Aliases: Ifg; IFN-g; Ifng

View more View less

UniProt ID: (Mouse) P01580

View more View less

Entrez Gene ID: (Mouse) 15978

View more View less

Function(s)

cytokine activity interferon-gamma receptor binding protein binding cytokine interferon superfamily intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IFN gamma Monoclonal Antibody (XMG1.2), Alexa Fluor™ 700, eBioscience™

IFN gamma Antibody (56-7311-82) in Flow

Product Details

56-7311-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!