Invitrogen

TNF alpha Monoclonal Antibody (MP6-XT22), PE-eFluor™ 610, eBioscience™

63 Published Figures
53 References
View all (210) TNF alpha antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

61-7321-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.25 µg/test
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Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MP6-XT22

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574666

Product Specific Information

Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Applications Reported: This MP6-XT22 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MP6-XT22 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) iintracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

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Gene Aliases: DIF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2

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UniProt ID: (Mouse) P06804

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Entrez Gene ID: (Mouse) 21926

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Function(s)
protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding
Process(es)
protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPKKK activity activation of MAPK activity cell activation positive regulation of cytokine production regulation of protein phosphorylation positive regulation of protein phosphorylation negative regulation of L-glutamate transport chronic inflammatory response to antigenic stimulus negative regulation of cytokine secretion involved in immune response positive regulation of chronic inflammatory response to antigenic stimulus positive regulation of humoral immune response mediated by circulating immunoglobulin glucose metabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process obsolete transformed cell apoptotic process defense response inflammatory response immune response humoral immune response signal transduction JNK cascade multicellular organism development cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to virus organ morphogenesis positive regulation of gene expression negative regulation of gene expression negative regulation of alkaline phosphatase activity calcium-mediated signaling extracellular matrix organization osteoclast differentiation sequestering of triglyceride positive regulation of protein complex assembly positive regulation of fever generation lipopolysaccharide-mediated signaling pathway negative regulation of interleukin-6 production positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-18 production positive regulation of interleukin-6 production receptor biosynthetic process positive regulation of peptidyl-serine phosphorylation tumor necrosis factor-mediated signaling pathway positive regulation of heterotypic cell-cell adhesion regulation of cell proliferation positive regulation of NF-kappaB import into nucleus defense response to bacterium positive regulation of apoptotic process positive regulation of programmed cell death regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of protein complex disassembly positive regulation of protein complex disassembly positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity protein kinase B signaling positive regulation of JUN kinase activity positive regulation of neuron apoptotic process negative regulation of growth of symbiont in host negative regulation of viral genome replication positive regulation of chemokine biosynthetic process cellular extravasation positive regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process negative regulation of myoblast differentiation negative regulation of osteoblast differentiation regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of action potential positive regulation of cell adhesion positive regulation of mitotic nuclear division positive regulation of protein kinase activity negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation by iron negative regulation of glucose import positive regulation of JNK cascade positive regulation of smooth muscle cell proliferation regulation of protein secretion positive regulation of cytokine secretion positive regulation of inflammatory response positive regulation of phagocytosis regulation of insulin secretion positive regulation of synaptic transmission defense response to Gram-positive bacterium leukocyte migration leukocyte tethering or rolling detection of mechanical stimulus involved in sensory perception of pain negative regulation of lipid catabolic process regulation of immunoglobulin secretion positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of protein transport response to glucocorticoid positive regulation of NFAT protein import into nucleus positive regulation of hair follicle development positive regulation of protein kinase B signaling positive regulation of vitamin D biosynthetic process positive regulation of calcidiol 1-monooxygenase activity epithelial cell proliferation involved in salivary gland morphogenesis regulation of branching involved in salivary gland morphogenesis negative regulation of branching involved in lung morphogenesis cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to nicotine cellular response to organic cyclic compound positive regulation of podosome assembly apoptotic signaling pathway extrinsic apoptotic signaling pathway necroptotic signaling pathway positive regulation of protein localization to cell surface positive regulation of chemokine (C-X-C motif) ligand 2 production regulation of reactive oxygen species metabolic process negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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