Invitrogen

CD140a (PDGFRA) Monoclonal Antibody (APA5), Super Bright™ 702, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

37 Published Figures

33 References

View all (78) PDGFRA (CD140a) antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

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CD140a (PDGFRA) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 39

CD140a (PDGFRA) Antibody (67-1401-82) in Flow

Staining of NIH/3T3 cells with 0.5 µg of Rat IgG2a K Isotype Control, Super Bright 702 (Product # 67-4321-82) (blue histogram) or 0.5 µg of CD140a (PDGF Receptor a) Monoclonal Antibody, Super Bright 702 (purple histogram). Total viable cells were used for analysis.

CD140a (PDGFRA) Antibody in Immunocytochemistry (ICC/IF)

CD140a (PDGFRA) Antibody in Immunohistochemistry (IHC)

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Product Details

67-1401-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

1.0 µg/test

View 23 publications 23 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 702, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

APA5

Conjugate

Super Bright™ 702 Super Bright™ 702 Super Bright™ 702

Excitation/Emission Max

413/702 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2717165

Product Specific Information

Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.

Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Distinct niche structures and intrinsic programs of fallopian tube and ovarian surface epithelial cells.

iScience

Qin G,Park ES,Chen X,Han S,Xiang D,Ren F,Liu G,Chen H,Yuan GC,Li Z

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Fri Jan 20 00:00:00 EST 2023

The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency.

Nature communications

Athanasouli P,Balli M,De Jaime-Soguero A,Boel A,Papanikolaou S,van der Veer BK,Janiszewski A,Vanhessche T,Francis A,El Laithy Y,Nigro AL,Aulicino F,Koh KP,Pasque V,Cosma MP,Verfaillie C,Zwijsen A,Heindryckx B,Nikolaou C,Lluis F

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Fri Mar 03 00:00:00 EST 2023

An estrogen-sensitive fibroblast population drives abdominal muscle fibrosis in an inguinal hernia mouse model.

JCI insight

Potluri T,Taylor MJ,Stulberg JJ,Lieber RL,Zhao H,Bulun SE

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Tue Apr 19 00:00:00 EDT 2022

Hematopoietic-Mesenchymal Signals Regulate the Properties of Mesenchymal Stem Cells.

International journal of molecular sciences

Kanazawa S,Okada H,Riu D,Mabuchi Y,Akazawa C,Iwata J,Hoshi K,Hikita A

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Tue Jul 26 00:00:00 EDT 2022

PEAR1 regulates expansion of activated fibroblasts and deposition of extracellular matrix in pulmonary fibrosis.

Nature communications

Geng Y,Li L,Yan J,Liu K,Yang A,Zhang L,Shen Y,Gao H,Wu X,Noth I,Huang Y,Liu J,Fan X

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Sat Nov 19 00:00:00 EST 2022

Lamin A/C-dependent chromatin architecture safeguards naïve pluripotency to prevent aberrant cardiovascular cell fate and function.

Nature communications

Wang Y,Elsherbiny A,Kessler L,Cordero J,Shi H,Serke H,Lityagina O,Trogisch FA,Mohammadi MM,El-Battrawy I,Backs J,Wieland T,Heineke J,Dobreva G

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Fri Nov 04 00:00:00 EDT 2022

Placental endocrine function shapes cerebellar development and social behavior.

Nature neuroscience

Vacher CM,Lacaille H,O'Reilly JJ,Salzbank J,Bakalar D,Sebaoui S,Liere P,Clarkson-Paredes C,Sasaki T,Sathyanesan A,Kratimenos P,Ellegood J,Lerch JP,Imamura Y,Popratiloff A,Hashimoto-Torii K,Gallo V,Schumacher M,Penn AA

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Immunohistochemistry

Fri Oct 01 00:00:00 EDT 2021

IGF1R controls mechanosignaling in myofibroblasts required for pulmonary alveologenesis.

JCI insight

He H,Snowball J,Sun F,Na CL,Whitsett JA

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Mon Mar 22 00:00:00 EDT 2021

Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer.

Nature communications

Nishina T,Deguchi Y,Ohshima D,Takeda W,Ohtsuka M,Shichino S,Ueha S,Yamazaki S,Kawauchi M,Nakamura E,Nishiyama C,Kojima Y,Adachi-Akahane S,Hasegawa M,Nakayama M,Oshima M,Yagita H,Shibuya K,Mikami T,Inohara N,Matsushima K,Tada N,Nakano H

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Fri Apr 16 00:00:00 EDT 2021

Zbtb16 regulates social cognitive behaviors and neocortical development.

Translational psychiatry

Usui N,Berto S,Konishi A,Kondo M,Konopka G,Matsuzaki H,Shimada S

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Immunohistochemistry

Sat Apr 24 00:00:00 EDT 2021

Gain-of-function p53 protein transferred via small extracellular vesicles promotes conversion of fibroblasts to a cancer-associated phenotype.

Cell reports

Ma S,McGuire MH,Mangala LS,Lee S,Stur E,Hu W,Bayraktar E,Villar-Prados A,Ivan C,Wu SY,Yokoi A,Dasari SK,Jennings NB,Liu J,Lopez-Berestein G,Ram P,Sood AK

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Tue Feb 09 00:00:00 EST 2021

Dlx5-augmentation in neural crest cells reveals early development and differentiation potential of mouse apical head mesenchyme.

Scientific reports

Vu TH,Takechi M,Shimizu M,Kitazawa T,Higashiyama H,Iwase A,Kurihara H,Iseki S

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Immunohistochemistry

Fri Jan 22 00:00:00 EST 2021

Extracellular vesicles synchronize cellular phenotypes of differentiating cells.

Journal of extracellular vesicles

Minakawa T,Matoba T,Ishidate F,Fujiwara TK,Takehana S,Tabata Y,Yamashita JK

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Immunocytochemistry

Wed Sep 01 00:00:00 EDT 2021

Extracellular vesicles synchronize cellular phenotypes of differentiating cells.

Journal of extracellular vesicles

Minakawa T,Matoba T,Ishidate F,Fujiwara TK,Takehana S,Tabata Y,Yamashita JK

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Immunocytochemistry

Wed Sep 01 00:00:00 EDT 2021

Mesothelial cells are not a source of adipocytes in mice.

Cell reports

Westcott GP,Emont MP,Li J,Jacobs C,Tsai L,Rosen ED

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Tue Jul 13 00:00:00 EDT 2021

Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung.

Developmental cell

Vila Ellis L,Cain MP,Hutchison V,Flodby P,Crandall ED,Borok Z,Zhou B,Ostrin EJ,Wythe JD,Chen J

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Immunocytochemistry

Mon Mar 09 00:00:00 EDT 2020

A smooth muscle-derived, Braf-driven mouse model of gastrointestinal stromal tumor (GIST): evidence for an alternative GIST cell-of-origin.

The Journal of pathology

Kondo J,Huh WJ,Franklin JL,Heinrich MC,Rubin BP,Coffey RJ

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Immunocytochemistry

Tue Dec 01 00:00:00 EST 2020

Hyaluronan Degradation by Cemip Regulates Host Defense against Staphylococcus aureus Skin Infection.

Cell reports

Dokoshi T,Zhang LJ,Li F,Nakatsuji T,Butcher A,Yoshida H,Shimoda M,Okada Y,Gallo RL

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Tue Jan 07 00:00:00 EST 2020

Tissue-Resident PDGFRα⁺ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner.

Cell reports

Santini MP,Malide D,Hoffman G,Pandey G,D'Escamard V,Nomura-Kitabayashi A,Rovira I,Kataoka H,Ochando J,Harvey RP,Finkel T,Kovacic JC

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

Loss of Fas signaling in fibroblasts impairs homeostatic fibrosis resolution and promotes persistent pulmonary fibrosis.

JCI insight

Redente EF,Chakraborty S,Sajuthi S,Black BP,Edelman BL,Seibold MA,Riches DW

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2020

Adipocyte Piezo1 mediates obesogenic adipogenesis through the FGF1/FGFR1 signaling pathway in mice.

Nature communications

Wang S,Cao S,Arhatte M,Li D,Shi Y,Kurz S,Hu J,Wang L,Shao J,Atzberger A,Wang Z,Wang C,Zang W,Fleming I,Wettschureck N,Honoré E,Offermanns S

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Fri May 08 00:00:00 EDT 2020

Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells.

PloS one

Falero-Perez J,Sheibani N,Sorenson CM

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Fri Jul 31 00:00:00 EDT 2020

Cardiac fibroblast proliferation rates and collagen expression mature early and are unaltered with advancing age.

JCI insight

Wu R,Ma F,Tosevska A,Farrell C,Pellegrini M,Deb A

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Thu Dec 17 00:00:00 EST 2020

Phosphatase SHP1 impedes mesenchymal stromal cell immunosuppressive capacity modulated by JAK1/STAT3 and P38 signals.

Cell & bioscience

Jiang M,Ye J,Wang X,Li N,Wang Y,Shi Y

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Thu Apr 14 00:00:00 EDT 2022

Interstitial cells of Cajal in W^sh/W^sh c-kit mutant mice.

Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi

Iino S,Horiguchi S,Horiguchi K,Hashimoto T

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Immunohistochemistry

Fri Jul 16 00:00:00 EDT 2021

Expression Profiling of Fibroblasts in Chronic and Acute Disease Models Reveals Novel Pathways in Kidney Fibrosis.

Journal of the American Society of Nephrology : JASN

Higashi AY,Aronow BJ,Dressler GR

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Immunohistochemistry

Tue Jan 01 00:00:00 EST 2019

Dual lineage tracing identifies intermediate mesenchymal stage for endocardial contribution to fibroblasts, coronary mural cells, and adipocytes.

The Journal of biological chemistry

Huang X,Feng T,Jiang Z,Meng J,Kou S,Lu Z,Chen W,Lin CP,Zhou B,Zhang H

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Fri May 31 00:00:00 EDT 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Tue Mar 05 00:00:00 EST 2019

A Brain-Melanocortin-Vagus Axis Mediates Adipose Tissue Expansion Independently of Energy Intake.

Cell reports

Holland J,Sorrell J,Yates E,Smith K,Arbabi S,Arnold M,Rivir M,Morano R,Chen J,Zhang X,Dimarchi R,Woods SC,Sanchez-Gurmaches J,Wohleb E,Perez-Tilve D

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Tue May 21 00:00:00 EDT 2019

Bone marrow-derived fibroblasts are a functionally distinct stromal cell population in breast cancer.

The Journal of experimental medicine

Raz Y,Cohen N,Shani O,Bell RE,Novitskiy SV,Abramovitz L,Levy C,Milyavsky M,Leider-Trejo L,Moses HL,Grisaru D,Erez N

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Immunohistochemistry

Mon Dec 03 00:00:00 EST 2018

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Tue Dec 11 00:00:00 EST 2018

Attenuated PDGF signaling drives alveolar and microvascular defects in neonatal chronic lung disease.

EMBO molecular medicine

Oak P,Pritzke T,Thiel I,Koschlig M,Mous DS,Windhorst A,Jain N,Eickelberg O,Foerster K,Schulze A,Goepel W,Reicherzer T,Ehrhardt H,Rottier RJ,Ahnert P,Gortner L,Desai TJ,Hilgendorff A

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Immunocytochemistry

Wed Nov 01 00:00:00 EDT 2017

The Isl1/Ldb1 Complex Orchestrates Genome-wide Chromatin Organization to Instruct Differentiation of Multipotent Cardiac Progenitors.

Cell stem cell

Caputo L,Witzel HR,Kolovos P,Cheedipudi S,Looso M,Mylona A,van IJcken WF,Laugwitz KL,Evans SM,Braun T,Soler E,Grosveld F,Dobreva G

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 67-1401-82) in Flow Cytometry

Thu Sep 03 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Alpha platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF alpha chain; PDGF Receptor alpha; PDGF-R-alpha; pdgfr-a; PDGFR-alpha; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor alpha

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Gene Aliases: AI115593; CD140a; Pdgfr-2; Pdgfra

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UniProt ID: (Mouse) P26618

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Entrez Gene ID: (Mouse) 18595

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity platelet-derived growth factor alpha-receptor activity vascular endothelial growth factor-activated receptor activity platelet-derived growth factor receptor binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding vascular endothelial growth factor binding protein homodimerization activity phosphatidylinositol 3-kinase binding platelet-derived growth factor binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD140a (PDGFRA) Monoclonal Antibody (APA5), Super Bright™ 702, eBioscience™

CD140a (PDGFRA) Antibody (67-1401-82) in Flow

Product Details

67-1401-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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