Invitrogen

CD14 Monoclonal Antibody (61D3), Super Bright™ 780, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

16 Published Figures

21 References

View all (206) CD14 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 18

CD14 Antibody (78-0149-42) in Flow

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD16 PE and CD14 Super Bright 780. As expected based on known relative expression patterns, CD14 clone 61D3 stains monocytes (orange) and granulocytes (blue), but not lymphocytes (pink).

CD14 Antibody in Immunocytochemistry (ICC/IF)

CD14 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD14 Antibody — Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD16 PE and CD14 Super Bright 780. As expected based on known relative expression patterns, CD14 clone 61D3 stains monocytes (orange) and granulocytes (blue), but not lymphocytes (pink). {RE}

Product Details

78-0149-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 17 publications 17 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

61D3

Conjugate

Super Bright™ 780 Super Bright™ 780 Super Bright™ 780

Excitation/Emission Max

413/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2734878

Product Specific Information

Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.

Applications Reported: This 61D3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 61D3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497).

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Saharan dust induces NLRP3-dependent inflammatory cytokines in an alveolar air-liquid interface co-culture model.

Particle and fibre toxicology

Bredeck G,Dobner J,Stahlmecke B,Fomba KW,Herrmann H,Rossi A,Schins RPF

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

LAL deficiency induced myeloid-derived suppressor cells as targets and biomarkers for lung cancer.

Journal for immunotherapy of cancer

Zhao T,Liu S,Hanna NH,Jalal S,Ding X,Wan J,Yan C,Du H

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Spontaneous Osteoclastogenesis, a risk factor for bone metastasis in advanced luminal A-type breast cancer patients.

Frontiers in oncology

Fernández Vallone V,Borzone FR,Martinez LM,Giorello MB,Choi H,Dimase F,Feldman L,Bordenave RH,Chudzinski-Tavassi AM,Batagelj E,Chasseing NA

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Fri Mar 10 00:00:00 EST 2023

SARS-CoV-2 Delta (B.1.617.2) variant replicates and induces syncytia formation in human induced pluripotent stem cell-derived macrophages.

PeerJ

Thaweerattanasinp T,Wanitchang A,Saenboonrueng J,Srisutthisamphan K,Wanasen N,Sungsuwan S,Jongkaewwattana A,Chailangkarn T

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2023

Heliangin acts as a covalent ligand of RPS2 that disrupts pre-rRNA metabolic processes in NPM1-mutated acute myeloid leukemia.

Acta pharmaceutica Sinica. B

Feng Y,Han Y,Hu A,Qu Y,Hu Y,Wu H,Wang X,He L

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2023

Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling.

Nature communications

Orliaguet L,Ejlalmanesh T,Humbert A,Ballaire R,Diedisheim M,Julla JB,Chokr D,Cuenco J,Michieletto J,Charbit J,Lindén D,Boucher J,Potier C,Hamimi A,Lemoine S,Blugeon C,Legoix P,Lameiras S,Baudrin LG,Baulande S,Soprani A,Castelli FA,Fenaille F,Riveline JP,Dalmas E,Rieusset J,Gautier JF,Venteclef N,Alzaid F

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Immunocytochemistry

Tue Aug 30 00:00:00 EDT 2022

A Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Assay Identifies Nilotinib as an Inhibitor of Inflammation in Acute Myeloid Leukemia.

Journal of medicinal chemistry

Marín-Rubio JL,Peltier-Heap RE,Dueñas ME,Heunis T,Dannoura A,Inns J,Scott J,Simpson AJ,Blair HJ,Heidenreich O,Allan JM,Watt JE,Martin MP,Saxty B,Trost M

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Thu Sep 22 00:00:00 EDT 2022

Decoding the pathogenesis of Diamond-Blackfan anemia using single-cell RNA-seq.

Cell discovery

Wang B,Wang C,Wan Y,Gao J,Ma Y,Zhang Y,Tong J,Zhang Y,Liu J,Chang L,Xu C,Shen B,Chen Y,Jiang E,Kurita R,Nakamura Y,Lim KC,Engel JD,Zhou J,Cheng T,Zhu X,Zhu P,Shi L

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Tue May 10 00:00:00 EDT 2022

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Exposure of a specific pleioform of multifunctional glyceraldehyde 3-phosphate dehydrogenase initiates CD14-dependent clearance of apoptotic cells.

Cell death & disease

Chaudhary S,Patidar A,Dhiman A,Chaubey GK,Dilawari R,Talukdar S,Modanwal R,Raje M

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Neutralization

Thu Sep 30 00:00:00 EDT 2021

The long non-coding RNA Cancer Susceptibility 15 (CASC15) is induced by isocitrate dehydrogenase (IDH) mutations and maintains an immature phenotype in adult acute myeloid leukemia.

Haematologica

Grasedieck S,Ruess C,Krowiorz K,Lux S,Pochert N,Schwarzer A,Klusmann JH,Jongen-Lavrencic M,Herold T,Bullinger L,Pollack JR,Rouhi A,Kuchenbauer F

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation.

PLoS pathogens

Oliveira AER,Pereira MCA,Belew AT,Ferreira LRP,Pereira LMN,Neves EGA,Nunes MDCP,Burleigh BA,Dutra WO,El-Sayed NM,Gazzinelli RT,Teixeira SMR

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

A functional antibody cross-reactive to both human and murine cytotoxic T-lymphocyte-associated protein 4 via binding to an N-glycosylation epitope.

mAbs

Li D,Li J,Chu H,Wang Z

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Fri Feb 12 00:00:00 EST 2021

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

MiR-103 protects from recurrent spontaneous abortion via inhibiting STAT1 mediated M1 macrophage polarization.

International journal of biological sciences

Zhu X,Liu H,Zhang Z,Wei R,Zhou X,Wang Z,Zhao L,Guo Q,Zhang Y,Chu C,Wang L,Li X

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Fri Nov 12 00:00:00 EST 2021

Patient iPSC-Derived Macrophages to Study Inborn Errors of the IFN-γ Responsive Pathway.

Cells

Haake K,Neehus AL,Buchegger T,Kühnel MP,Blank P,Philipp F,Oleaga-Quintas C,Schulz A,Grimley M,Goethe R,Jonigk D,Kalinke U,Boisson-Dupuis S,Casanova JL,Bustamante J,Lachmann N

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Wed Feb 19 00:00:00 EST 2020

Chemerin enhances the adhesion and migration of human endothelial progenitor cells and increases lipid accumulation in mice with atherosclerosis.

Lipids in health and disease

Jia J,Yu F,Xiong Y,Wei W,Ma H,Nisi F,Song X,Yang L,Wang D,Yuan G,Zhou H

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Sun Sep 20 00:00:00 EDT 2020

NLRP3 Inflammasome Activation in Hemodialysis and Hypertensive Patients with Intact Kidney Function.

Toxins

Ulrich C,Wildgrube S,Fiedler R,Seibert E,Kneser L,Fick S,Schäfer C,Markau S,Trojanowicz B,Girndt M

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Mon Oct 26 00:00:00 EDT 2020

LILRB3 (ILT5) is a myeloid cell checkpoint that elicits profound immunomodulation.

JCI insight

Yeboah M,Papagregoriou C,Jones DC,Chan HTC,Hu G,McPartlan JS,Schiött T,Mattson U,Mockridge CI,Tornberg UC,Hambe B,Ljungars A,Mattsson M,Tews I,Glennie MJ,Thirdborough SM,Trowsdale J,Frendeus B,Chen J,Cragg MS,Roghanian A

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Thrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.

Scientific reports

Tsai CS,Lin YW,Huang CY,Shih CM,Tsai YT,Tsao NW,Lin CS,Shih CC,Jeng H,Lin FY

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Immunocytochemistry

Fri Dec 02 00:00:00 EST 2016

Biologically active polymers from spontaneous carotenoid oxidation: a new frontier in carotenoid activity.

PloS one

Johnston JB,Nickerson JG,Daroszewski J,Mogg TJ,Burton GW

Published figure using CD14 monoclonal antibody (Product # 78-0149-42) in Immunohistochemistry

Thu Jun 25 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

View more View less

Gene Aliases: CD14

View more View less

UniProt ID: (Human) P08571

View more View less

Entrez Gene ID: (Human) 929

View more View less

Function(s)

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding transmembrane signal receptor

Process(es)

MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway receptor-mediated endocytosis phagocytosis apoptotic process inflammatory response cell surface receptor signaling pathway I-kappaB kinase/NF-kappaB signaling response to heat lipopolysaccharide-mediated signaling pathway response to magnesium ion positive regulation of type I interferon production positive regulation of interferon-gamma production positive regulation of tumor necrosis factor production negative regulation of MyD88-independent toll-like receptor signaling pathway toll-like receptor 4 signaling pathway response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway innate immune response response to ethanol positive regulation of endocytosis positive regulation of cytokine secretion response to electrical stimulus necroptotic process cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD14 Monoclonal Antibody (61D3), Super Bright™ 780, eBioscience™

CD14 Antibody (78-0149-42) in Flow

Product Details

78-0149-42

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!