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Invitrogen
CD134 (OX40) Monoclonal Antibody (OX-86), Super Bright™ 780, eBioscience™
FIGURE: 1 / 2
CD134 (OX40) Antibody (78-1341-82) in Flow
Staining of unstimulated (left) or 3-day Con A (00-4978-93)-stimulated C57Bl/6 mouse splenocytes (right) with 1.0 µg of Rat IgG1 kappa Isotype Control, Super Bright 780 (Product # 78-4301-82) (blue histogram) or 1.0 µg of CD134 (OX40) Monoclonal Antibody, Super Bright 780 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.
Product Details
78-1341-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
OX-86
Conjugate
Excitation/Emission Max
413/780 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2734876
Product Specific Information
Description: The OX-86 monoclonal antibody reacts with mouse CD134, also known as OX40. A member of the TNF receptor superfamily, CD134 is a 50 kDa type I membrane glycoprotein expressed by activated mouse T lymphocytes. Rat CD134 was initially identified as an activation marker only on activated CD4+ T cells. In contrast, mouse CD134 is expressed by both activated CD4+ and CD8+ T cells. The interaction of CD134 with CD252 (OX40 ligand) has been implicated in T cell-dependent humoral responses, regulation of primary T cell expansion, survival of T cells, size of the memory T cell pool, and regulation of tolerance in the CD4+ T cell compartment.
Applications Reported: The OX-86 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The OX-86 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497).
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
OX40 is a protein receptor found on the surface of T cells, a type of white blood cell that plays a crucial role in the immune system. When activated, OX40 promotes the proliferation and survival of T cells, leading to a stronger immune response against cancer cells and infectious agents. OX40 agonists, which are drugs that activate OX40, have shown promising results in preclinical and clinical studies as a potential immunotherapy for cancer. They can enhance the efficacy of other cancer treatments such as chemotherapy and checkpoint inhibitors, and may also have applications in autoimmune diseases and allergies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD134; OX40 antigen; OX40L receptor; tax-transcriptionally activated glycoprotein 1; Tumor necrosis factor receptor superfamily member 4
Gene Aliases: ACT35; CD134; Ly-70; Ox40; Tnfrsf4; Txgp1; TXGP1L
UniProt ID: (Mouse) P47741
Entrez Gene ID: (Mouse) 22163
inflammatory response
cellular defense response
programmed cell death
positive regulation of B cell proliferation
tumor necrosis factor-mediated signaling pathway
T cell proliferation
regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
regulation of protein kinase activity
negative regulation of transcription, DNA-templated
negative regulation of cytokine secretion
positive regulation of immunoglobulin secretion
negative regulation of activation-induced cell death of T cells
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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