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AF6 Antibody (A302-199A-T) in WB
Detection of human and mouse AF6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AF6 antibody A302-199A-M (lot A302-199A-M-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A302-199A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Between 1475 and 1525
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
AFDN belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Afadin; Afadin adherens junction formation factor; ALL1-fused gene from chromosome 6 protein; FLJ34371; myeloid/lymphoid or mixed lineage-leukemia translocation to 4 homolog; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4; myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4; Protein AF-6; RP3-431P23.3; RP3-470B24.4
Gene Aliases: 5033403D15Rik; Af-6; AF6; Afadin; AFDN; Gm314; I-afadin; MLL-AF6; MLLT4; S-afadin
UniProt ID: (Human) P55196, (Mouse) Q9QZQ1
Entrez Gene ID: (Human) 4301, (Mouse) 17356
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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