Invitrogen

IGF1R alpha Monoclonal Antibody (24-31)

Advanced Verification

IP-mass spectrometry

3 Published Figures

8 References

View all (15) IGF1R alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

IGF1R alpha Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

IGF1R alpha Antibody (AHR0321) in ICC/IF

Immunofluorescent analysis of IGF-1 Receptor alpha (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an IGF-1 Receptor alpha monoclonal antibody (Product # AH... View More

IGF1R alpha Antibody in Immunohistochemistry (IHC)

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Product Details

AHR0321

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

2 µg / mg lysate

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

24-31

Immunogen

IGF-1R/3T3 mouse fibroblasts transfected with human type 1 IGF-receptor cDNA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536352

Product Specific Information

This antibody reacts with an epitope at aa 283-440 (exon 4-6).

Recommended positive controls include placenta or breast tissue. This antibody is not suitable for Western blot. Without BSA, this antibody can be used as a capture antibody in a sandwich ELISA.

Target Information

IGF1R (Insulin-like Growth Factor1 Receptor) is a receptor tyrosine kinase that mediates actions of insulin-like growth factor 1 (IFG1). It binds IGF1 with high affinity and IGF2/insulin with lower affinity. Activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survial of malignant cells. Ligand binding activates the receptor kinase and leads to receptor autophosphorylation and tyrosines phosphorylation of multiple substrates that function as signaling adapter proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities.

Nature

Han K,Pierce SE,Li A,Spees K,Anderson GR,Seoane JA,Lo YH,Dubreuil M,Olivas M,Kamber RA,Wainberg M,Kostyrko K,Kelly MR,Yousefi M,Simpkins SW,Yao D,Lee K,Kuo CJ,Jackson PK,Sweet-Cordero A,Kundaje A,Gentles AJ,Curtis C,Winslow MM,Bassik MC

AHR0321 was used in Immunocytochemistry-Immunofluorescence to reveal key differences between 2D and 3D cancer models, and establish a generalizable strategy for performing CRISPR screens in spheroids to reveal cancer vulnerabilities.

Wed Apr 01 00:00:00 EDT 2020

CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities.

Nature

Wed Apr 01 00:00:00 EDT 2020

Targeting the insulin growth factor-1 receptor with fluorescent antibodies enables high resolution imaging of human pancreatic cancer in orthotopic mouse models.

Oncotarget

Park JY,Lee JY,Zhang Y,Hoffman RM,Bouvet M

AHR0321 was used in immunocytochemistry and immunohistochemistry - frozen section to test if fluorescently labeled insulin-like growth factor-1 receptor antibodies can be used to visualize pancreatic cancer in orthotopic nude mouse models

Tue Apr 05 00:00:00 EDT 2016

Fluorescent-Antibody Targeting of Insulin-Like Growth Factor-1 Receptor Visualizes Metastatic Human Colon Cancer in Orthotopic Mouse Models.

PloS one

Park JY,Murakami T,Lee JY,Zhang Y,Hoffman RM,Bouvet M

AHR0321 was used in immunocytochemistry to test if insulin-like growth factor-1 receptor antibodies enable the visualization of metastatic colon cancer in orthotopic nude mouse models

Thu Jun 30 00:00:00 EDT 2016

Metabolic syndrome and colorectal cancer: is hyperinsulinemia/insulin receptor-mediated angiogenesis a critical process?

Journal of geriatric oncology

Liu JJ,Druta M,Shibata D,Coppola D,Boler I,Elahi A,Reich RR,Siegel E,Extermann M

AHR0321 was used in immunohistochemistry - paraffin section to discuss mechanisms that link metabolic syndrome to colorectal cancer risk

Wed Jan 01 00:00:00 EST 2014

Differential expression of the insulin-like growth factor receptor among early breast cancer subtypes.

PloS one

Mountzios G,Aivazi D,Kostopoulos I,Kourea HP,Kouvatseas G,Timotheadou E,Zebekakis P,Efstratiou I,Gogas H,Vamvouka C,Chrisafi S,Stofas A,Pentheroudakis G,Koutras A,Galani E,Bafaloukos D,Fountzilas G

AHR0321 was used in Immunohistochemistry to determine the level of protein expression of the critical components of the insulin-like growth factor receptor pathway and evaluate their prognostic significance across the different early breast cancer subtypes.

Tue Jan 06 00:00:00 EST 2015

Increased signalling of EGFR and IGF1R, and deregulation of PTEN/PI3K/Akt pathway are related with trastuzumab resistance in HER2 breast carcinomas.

British journal of cancer

Gallardo A,Lerma E,Escuin D,Tibau A,Muñoz J,Ojeda B,Barnadas A,Adrover E,Sánchez-Tejada L,Giner D,Ortiz-Martínez F,Peiró G

AHR0321 was used in immunohistochemistry - paraffin section to investigate the contribution of the PI3K/Akt pathway to trastuzumab resistance

Tue Apr 10 00:00:00 EDT 2012

An ER activity profile including ER, PR, Bcl-2 and IGF-IR may have potential as selection criterion for letrozole or tamoxifen treatment of patients with advanced breast cancer.

Acta oncologica (Stockholm, Sweden)

Henriksen KL,Rasmussen BB,Lykkesfeldt AE,Møller S,Ejlertsen B,Mouridsen HT

AHR0321 was used in Immunohistochemistry to evaluate the association between response at recurrence to letrozole versus tamoxifen and the expression of oestrogen regulated proteins.

Tue May 12 00:00:00 EDT 2009

The pT1a and pT1b category subdivision in renal cell carcinoma: is it reflected by differences in tumour biology?

BJU international

Langner C,Ratschek M,Rehak P,Tsybrovskyy O,Zigeuner R

AHR0321 was used in immunohistochemistry - paraffin section to study the category subdivision of pT1a and pT1b in renal cell carcinoma

Tue Feb 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: CD221; IGF-I receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2

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Gene Aliases: CD221; IGF1R; IGFIR; IGFR; JTK13

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UniProt ID: (Human) P08069

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Entrez Gene ID: (Human) 3480

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Function(s)

protein tyrosine kinase activity insulin-like growth factor-activated receptor activity insulin receptor binding protein binding insulin-like growth factor binding ATP binding insulin-like growth factor I binding identical protein binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding transmembrane signal receptor

Process(es)

immune response signal transduction positive regulation of cell proliferation insulin receptor signaling pathway phosphatidylinositol 3-kinase signaling positive regulation of cell migration peptidyl-tyrosine autophosphorylation negative regulation of apoptotic process positive regulation of DNA replication regulation of JNK cascade protein autophosphorylation insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling protein tetramerization inactivation of MAPKK activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IGF1R alpha Monoclonal Antibody (24-31)

IGF1R alpha Antibody (AHR0321) in ICC/IF

Product Details

AHR0321

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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