Invitrogen

IGF1R alpha Monoclonal Antibody (24-31), Biotin

1 Published Figure

21 References

View all (15) IGF1R alpha antibodies

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

IGF1R alpha Antibody in Immunocytochemistry (ICC/IF)

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IGF1R alpha Antibody (MA5-13799) in ICC/IF

Immunofluorescent analysis of IGF-1 Receptor alpha (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an IGF-1 Receptor alpha monoclonal antibody (Product # MA... View More

IGF1R alpha Antibody in Immunohistochemistry (IHC)

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Product Details

MA5-13799

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 18 publications 18 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

2 µg/mL

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

24-31

Immunogen

IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10981576

Product Specific Information

MA5-13799 targets IGF-1 Receptor alpha in ELISA, ICC/IF, IHC (P) and immunoprecipitation applications and shows reactivity with Human samples.

The MA5-13799 immunogen is iGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA.

Target Information

IGF1R (Insulin-like Growth Factor1 Receptor) is a receptor tyrosine kinase that mediates actions of insulin-like growth factor 1 (IFG1). It binds IGF1 with high affinity and IGF2/insulin with lower affinity. Activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survial of malignant cells. Ligand binding activates the receptor kinase and leads to receptor autophosphorylation and tyrosines phosphorylation of multiple substrates that function as signaling adapter proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Multiplexed Exchange-PAINT imaging reveals ligand-dependent EGFR and Met interactions in the plasma membrane.

Scientific reports

Werbin JL,Avendaño MS,Becker V,Jungmann R,Yin P,Danuser G,Sorger PK

MA5-13799 was used in Immunocytochemistry-immunoflourescence to measure the precise distributions of five receptor tyrosine kinases from the ErbB, IGF-1R and Met families in breast cancer cells.

Fri Sep 22 00:00:00 EDT 2017

Insulin-like growth factor 1 receptor (IGF1R) expression and survival in operable squamous-cell laryngeal cancer.

PloS one

Mountzios G,Kostopoulos I,Kotoula V,Sfakianaki I,Fountzilas E,Markou K,Karasmanis I,Leva S,Angouridakis N,Vlachtsis K,Nikolaou A,Konstantinidis I,Fountzilas G

MA5-13799 was used in immunohistochemistry to study the prognostic value of IGF-1R expression in operable squamous cell laryngeal cancer

Mon Jul 08 00:00:00 EDT 2013

Does insulin-like growth factor 1 receptor (IGF-1R) targeting provide new treatment options for chordomas? A retrospective clinical and immunohistochemical study.

Histopathology

Scheipl S,Froehlich EV,Leithner A,Beham A,Quehenberger F,Mokry M,Stammberger H,Varga PP,Lazáry A,Windhager R,Gattenloehner S,Liegl B

MA5-13799 was used in immunohistochemistry to study the therapeutic potential of targeting IGF-1R in chordoma

Tue May 01 00:00:00 EDT 2012

Utility of insulin-like growth factor receptor-1 expression in gefitinib-treated patients with non-small cell lung cancer.

Anticancer research

Fidler MJ,Basu S,Buckingham L,Walters K,McCormack S,Batus M,Coon J,Bonomi P

MA5-13799 was used in immunohistochemistry to study whether IGF1R expression in non-small cell lung cancer patients is linked to gefitinib resistance

Tue May 01 00:00:00 EDT 2012

Phase II study of preoperative systemic treatment with the combination of docetaxel and trastuzumab in patients with locally advanced HER-2-overexpressing breast cancer.

Breast (Edinburgh, Scotland)

Sawaki M,Iwata H,Sato Y,Wada M,Toyama T,Sasaki E,Yatabe Y,Imai T,Ohashi Y

MA5-13799 was used in immunohistochemistry to investigate the efficacy of docetaxel and trastuzumab against locally advanced HER-2-overexpressing breast cancer

Fri Oct 01 00:00:00 EDT 2010

High coexpression of both insulin-like growth factor receptor-1 (IGFR-1) and epidermal growth factor receptor (EGFR) is associated with shorter disease-free survival in resected non-small-cell lung cancer patients.

Annals of oncology : official journal of the European Society for Medical Oncology

Ludovini V,Bellezza G,Pistola L,Bianconi F,Di Carlo L,Sidoni A,Semeraro A,Del Sordo R,Tofanetti FR,Mameli MG,Daddi G,Cavaliere A,Tonato M,Crinò L

MA5-13799 was used in immunohistochemistry to investigate the prognostic significance of IGF-1 receptor and EGF receptor expression in resected non-small-cell lung cancer patients

Fri May 01 00:00:00 EDT 2009

Relationships of insulin-like growth factor-1 receptor and epidermal growth factor receptor expression to clinical outcomes in patients with colorectal cancer.

Oncology

Takahari D,Yamada Y,Okita NT,Honda T,Hirashima Y,Matsubara J,Takashima A,Kato K,Hamaguchi T,Shirao K,Shimada Y,Shimoda T

MA5-13799 was used in immunohistochemistry to investigate the prognostic significance of IGF-1 receptor and EGF receptor expression in colorectal cancer

Mon Jan 26 00:00:00 EST 2009

Impact of insulin-like growth factor type 1 receptor, epidermal growth factor receptor, and HER2 expressions on outcomes of patients with gastric cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Matsubara J,Yamada Y,Hirashima Y,Takahari D,Okita NT,Kato K,Hamaguchi T,Shirao K,Shimada Y,Shimoda T

MA5-13799 was used in immunohistochemistry to study the prognostic significance of IGF-1 receptor, HER2, and EGF receptor in gastric cancer

Thu May 15 00:00:00 EDT 2008

HoxA9 induces insulin-like growth factor-1 receptor expression in B-lineage acute lymphoblastic leukemia.

Leukemia

Whelan JT,Ludwig DL,Bertrand FE

MA5-13799 was used in immunoprecipitation and western blot to study the role of HoxA9 in inducing IGF-1R expression in B-lineage acute lymphoblastic leukemia

Sun Jun 01 00:00:00 EDT 2008

Predictors of resistance to preoperative trastuzumab and vinorelbine for HER2-positive early breast cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Harris LN,You F,Schnitt SJ,Witkiewicz A,Lu X,Sgroi D,Ryan PD,Come SE,Burstein HJ,Lesnikoski BA,Kamma M,Friedman PN,Gelman R,Iglehart JD,Winer EP

MA5-13799 was used in immunohistochemistry to study predictors of response to pre-operative trastuzumab and vinorelbine for HER2-positive early breast cancer

Thu Feb 15 00:00:00 EST 2007

Intratumoral IGF-I protein expression is selectively upregulated in breast cancer patients with BRCA1/2 mutations.

Endocrine-related cancer

Hudelist G,Wagner T,Rosner M,Fink-Retter A,Gschwantler-Kaulich D,Czerwenka K,Kroiss R,Tea M,Pischinger K,Köstler WJ,Attems J,Mueller R,Blaukopf C,Kubista E,Hengstschläger M,Singer CF

MA5-13799 was used in immunohistochemistry to investigate the expression of intratumoral IGF-I protein in breast cancer patients with BRCA1/2 mutations

Sat Dec 01 00:00:00 EST 2007

Semi-quantitative scoring of potentially predictive markers for endocrine treatment of breast cancer: a comparison between whole sections and tissue microarrays.

Journal of clinical pathology

Henriksen KL,Rasmussen BB,Lykkesfeldt AE,Møller S,Ejlertsen B,Mouridsen HT

MA5-13799 was used in immunohistochemistry to study the use of tissue microarrays for the semi-quantitative scoring of potentially predictive markers for endocrine treatment of breast cancer

Sun Apr 01 00:00:00 EDT 2007

Semi-quantitative scoring of potentially predictive markers for endocrine treatment of breast cancer: a comparison between whole sections and tissue microarrays.

Journal of clinical pathology

Henriksen KL,Rasmussen BB,Lykkesfeldt AE,Møller S,Ejlertsen B,Mouridsen HT

MA5-13799 was used in immunohistochemistry to study the use of tissue microarrays for the semi-quantitative scoring of potentially predictive markers for endocrine treatment of breast cancer

Sun Apr 01 00:00:00 EDT 2007

The p53 pathway and outcome among patients with T1G3 bladder tumors.

Clinical cancer research : an official journal of the American Association for Cancer Research

López-Knowles E,Hernández S,Kogevinas M,Lloreta J,Amorós A,Tardón A,Carrato A,Kishore S,Serra C,Malats N,Real FX

MA5-13799 was used in immunohistochemistry to study the prognostic value of p53 pathway expression in patients with T1G3 bladder tumors

Sun Oct 15 00:00:00 EDT 2006

Multiple bronchioloalveolar carcinomas in acromegaly: a potential role of insulin-like growth factor I in carcinogenesis.

Lung cancer (Amsterdam, Netherlands)

Motoi N,Kishi K,Fujii T,Tsuboi E,Ohashi K,Yoshimura K

MA5-13799 was used in immunohistochemistry to investigate the novel role of insulin-like growth factor I in carcinogenesis

Wed Nov 01 00:00:00 EST 2006

Increased hepatic expression of insulin-like growth factor-I receptor in chronic hepatitis C.

World journal of gastroenterology

Stefano JT,Correa-Giannella ML,Ribeiro CM,Alves VA,Massarollo PC,Machado MC,Giannella-Neto D

MA5-13799 was used in immunohistochemistry to investigate expression of insulin-like growth factor-I receptor and insulin-like growth factor-I in livers from patients with chronic hepatitis C

Wed Jun 28 00:00:00 EDT 2006

Insulin-like growth factor-1 receptor (IGF-1R) expression does not predict for resistance to trastuzumab-based treatment in patients with Her-2/neu overexpressing metastatic breast cancer.

Journal of cancer research and clinical oncology

Köstler WJ,Hudelist G,Rabitsch W,Czerwenka K,Müller R,Singer CF,Zielinski CC

MA5-13799 was used in immunohistochemistry to evaluate the clinical relevance of insulin-like growth factor-1 receptor expression within the context of trastuzumab-based therapy

Sun Jan 01 00:00:00 EST 2006

Retinoblastoma susceptibility gene product pRB activates hypoxia-inducible factor-1 (HIF-1).

Oncogene

Budde A,Schneiderhan-Marra N,Petersen G,Brüne B

MA5-13799 was used in ChIP assay to study the role of retinoblastoma susceptibility gene product I in hypoxia-inducible factor-1 induction

Thu Mar 03 00:00:00 EST 2005

The pT1a and pT1b category subdivision in renal cell carcinoma: is it reflected by differences in tumour biology?

BJU international

Langner C,Ratschek M,Rehak P,Tsybrovskyy O,Zigeuner R

MA5-13799 was used in immunohistochemistry to study the category subdivision of pT1a and pT1b in renal cell carcinoma

Tue Feb 01 00:00:00 EST 2005

Expression of tyrosine kinases in human malignancies as potential targets for kinase-specific inhibitors.

Endocrine-related cancer

Singer CF,Hudelist G,Lamm W,Mueller R,Czerwenka K,Kubista E

MA5-13799 was used in immunohistochemistry to study the expression of tyrosine kinases in human malignancies as potential targets for kinase-specific inhibitors

Wed Dec 01 00:00:00 EST 2004

Analysis of insulin-like growth factors and insulin-like growth factor I receptor expression in renal cell carcinoma.

American journal of clinical pathology

Schips L,Zigeuner R,Ratschek M,Rehak P,Rüschoff J,Langner C

MA5-13799 was used in immunohistochemistry to study the expression of IGF-IR and IGF I and II in renal cell carcinoma

Wed Dec 01 00:00:00 EST 2004

The role of beta-catenin, TGF beta 3, NGF2, FGF2, IGFR2, and BMP4 in the pathogenesis of mesenteric sclerosis and angiopathy in midgut carcinoids.

Human pathology

Zhang PJ,Furth EE,Cai X,Goldblum JR,Pasha TL,Min KW

MA5-13799 was used in immunohistochemistry to study the role of several fibrosing-related growth factor in the pathogenesis of mesenteric sclerosis and angiopathy in midgut carcinoids

Tue Jun 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: CD221; IGF-I receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2

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Gene Aliases: CD221; IGF1R; IGFIR; IGFR; JTK13

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UniProt ID: (Human) P08069

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Entrez Gene ID: (Human) 3480

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Function(s)

protein tyrosine kinase activity insulin-like growth factor-activated receptor activity insulin receptor binding protein binding insulin-like growth factor binding ATP binding insulin-like growth factor I binding identical protein binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding transmembrane signal receptor

Process(es)

immune response signal transduction positive regulation of cell proliferation insulin receptor signaling pathway phosphatidylinositol 3-kinase signaling positive regulation of cell migration peptidyl-tyrosine autophosphorylation negative regulation of apoptotic process positive regulation of DNA replication regulation of JNK cascade protein autophosphorylation insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling protein tetramerization inactivation of MAPKK activity

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IGF1R alpha Monoclonal Antibody (24-31), Biotin

IGF1R alpha Antibody (MA5-13799) in ICC/IF

Product Details

MA5-13799

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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