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ATG14 Antibody (701670) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa Serum starved (Lane2), HeLa serum starved and treated with Rapamycin (100nM/4 hours) (Lane3), A549 (Lane 4), A549 serum starved (Lane5), A549 serum starved treated with Rapamycin (100nM/ 4 hours) (Lane 6), U-87MG (Lane 7), U-87MG serum starved (Lane 8) and U-87MG serum starved and treated with Rapamycin (100nM/4 hours) (Lane 9). The blots were probed with Anti- ATG14 Recombinant Rabbit Monoclonal Antibody (Product # 701670, 1-2 µg/mL) and detec... View More
Product Details
701670
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
10H20L14
Immunogen
Multiple peptides corresponding to Human ATG14 (aa 63-79, 162-178, 277-293)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532500
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Mouse, Pig, Bovine, Rabbit and Dog.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
ATG14 (Autophagy related protein homolog 14) is encoded by the ATG14 gene and is located on chromosome 14 in humans. It is also known as ATG14L or BARKOR (Beclin 1-associated autophagy-related key regulator). Autophagy is an evolutionarily conserved process that involves recycling of misfolded cellular proteins and degradation of dysfunctional organelles. Autophagy is induced as a cellular response to nutrient stress which includes the formation of autophagosomes, fusion of these with the lysosome and formation of the autophagolysosome. The Class III PI3-Kinase, Vps34 and its interacting partner Beclin 1 have been shown to form a complex which specifically includes ATG 14 (type 1) or UVRAG (type 2). Atg 14 guides the type 1 complex to the preautophagosomal structure (PAS) and is critical for the initiation of autophagosome formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATG14 autophagy related 14 homolog; Atg14L; Autophagy-related protein 14-like protein; Bakor; Barkor; Beclin 1-associated autophagy-related key regulator; Beclin 1-Interacting protein
Gene Aliases: ATG14; ATG14L; BARKOR; KIAA0831
UniProt ID: (Human) Q6ZNE5
Entrez Gene ID: (Human) 22863
autophagosome assembly
regulation of protein phosphorylation
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
endosome to lysosome transport
cellular response to starvation
positive regulation of autophagy
posttranscriptional regulation of gene expression
macroautophagy
cellular response to glucose starvation
positive regulation of phosphatidylinositol 3-kinase activity
regulation of protein complex stability
regulation of triglyceride metabolic process
mitophagy in response to mitochondrial depolarization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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