Proteintech

ATG14/Barkor (N-terminal) Polyclonal Antibody

ATG14/Barkor (N-terminal) Antibody in Immunocytochemistry (ICC/IF)

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ATG14/Barkor (N-terminal) Antibody (19491-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using (Product # 19491-1-AP) (ATG14/Barkor (N-terminal) antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ATG14/Barkor (N-terminal) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATG14/Barkor (N-terminal) Antibody in Western Blot (WB)

ATG14/Barkor (N-terminal) Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

19491-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 20 publications 20 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Bovine, Dog, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to KIAA0831 (Barkor).

Target Information

ATG14 (Autophagy related protein homolog 14) is encoded by the ATG14 gene and is located on chromosome 14 in humans. It is also known as ATG14L or BARKOR (Beclin 1-associated autophagy-related key regulator). Autophagy is an evolutionarily conserved process that involves recycling of misfolded cellular proteins and degradation of dysfunctional organelles. Autophagy is induced as a cellular response to nutrient stress which includes the formation of autophagosomes, fusion of these with the lysosome and formation of the autophagolysosome. The Class III PI3-Kinase, Vps34 and its interacting partner Beclin 1 have been shown to form a complex which specifically includes ATG 14 (type 1) or UVRAG (type 2). Atg 14 guides the type 1 complex to the preautophagosomal structure (PAS) and is critical for the initiation of autophagosome formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATG14 autophagy related 14 homolog; Atg14-like protein; Atg14L; Autophagy-related protein 14-like protein; Bakor; Barkor; Beclin 1-associated autophagy-related key regulator; Beclin 1-Interacting protein; VATG14 autophagy related 14 homolog

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Gene Aliases: 4832427M01; ATG14; ATG14L; BARKOR; D14Ertd114e; D14Ertd436e; KIAA0831

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UniProt ID: (Human) Q6ZNE5, (Mouse) Q8CDJ3

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Entrez Gene ID: (Human) 22863, (Mouse) 100504663

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Function(s)

protein binding

Process(es)

autophagosome assembly regulation of protein phosphorylation negative regulation of protein phosphorylation positive regulation of protein phosphorylation endosome to lysosome transport cellular response to starvation positive regulation of autophagy posttranscriptional regulation of gene expression macroautophagy cellular response to glucose starvation positive regulation of phosphatidylinositol 3-kinase activity regulation of protein complex stability regulation of triglyceride metabolic process mitophagy in response to mitochondrial depolarization autophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATG14/Barkor (N-terminal) Polyclonal Antibody

ATG14/Barkor (N-terminal) Antibody (19491-1-AP) in ICC/IF

Product Details

19491-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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