Invitrogen

BrdU Monoclonal Antibody (MoBU-1), Pacific Blue™

FIGURE: 1 / 5

BrdU Antibody (B35129) in Flow

Jurkat T-cell leukemia cells were treated with 10µM BrdU for 1.5 hours. The cells were then fixed in ETOH and stored overnight at ≤-20°C. An acid denaturation method was used to prepare the cells before labeling with BrdU mouse monoclonal antibody (Clone MoBU-1) Pacific™ Blue *for flow cytometry* *100 tests* to detect the incorporated BrdU, each using 5 µL antibody conjugate labeling 1 x 10^6 cells. Proliferating cells are clearly distinguished from non-proliferating cells.

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Product Details

B35129

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 12 publications 12 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

MoBU-1

Immunogen

BrdU

Conjugate

Pacific Blue™ Pacific Blue™ Pacific Blue™

Excitation/Emission Max

404/455 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2536433

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Partial loss-of-function mutations in GINS4 lead to NK cell deficiency with neutropenia.

JCI insight

Conte MI,Poli MC,Taglialatela A,Leuzzi G,Chinn IK,Salinas SA,Rey-Jurado E,Olivares N,Veramendi-Espinoza L,Ciccia A,Lupski JR,Aldave Becerra JC,Mace EM,Orange JS

B35129 was used in Flow cytometry/Cell sorting to demonstrate biallelic partial loss-of-function mutations in GINS4, defining a potentially novel disease-causing gene underlying NKD with neutropenia.

Tue Nov 08 00:00:00 EST 2022

RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation.

Nature communications

Li T,Wang X,Ju E,da Silva SR,Chen L,Zhang X,Wei S,Gao SJ

B35129 was used in Flow Cytometry to study K29-linked polyubiquitination and degradation of CASTOR1 by E3 ubiquitin ligase RNF167.

Tue Feb 16 00:00:00 EST 2021

Kaposi sarcoma-associated herpesvirus miRNAs suppress CASTOR1-mediated mTORC1 inhibition to promote tumorigenesis.

The Journal of clinical investigation

Li T,Ju E,Gao SJ

B35129 was used in Western Blotting to define the mechanism of KSHV activation of the mTORC1 pathway and establish the scientific basis for targeting this pathway to treat KSHV-associated cancers.

Mon Jul 15 00:00:00 EDT 2019

CRISPR-Cas9 Screening of Kaposi's Sarcoma-Associated Herpesvirus-Transformed Cells Identifies XPO1 as a Vulnerable Target of Cancer Cells.

mBio

Gruffaz M,Yuan H,Meng W,Liu H,Bae S,Kim JS,Lu C,Huang Y,Gao SJ

B35129 was used in Flow cytometry/Cell sorting to elucidate the role of XPO1 in cell proliferation and growth transformation of KSHV-transformed cells and in cell lines of other cancers, including gastric cancer and liver cancer.

Tue May 14 00:00:00 EDT 2019

Non-lytic clearance of influenza B virus from infected cells preserves epithelial barrier function.

Nature communications

Dumm RE,Fiege JK,Waring BM,Kuo CT,Langlois RA,Heaton NS

B35129 was used in Flow cytometry/Cell sorting to determine if any cells can survive direct influenza B virus infection.

Fri Feb 15 00:00:00 EST 2019

Germinal Center B Cells Replace Their Antigen Receptors in Dark Zones and Fail Light Zone Entry when Immunoglobulin Gene Mutations are Damaging.

Immunity

Stewart I,Radtke D,Phillips B,McGowan SJ,Bannard O

B35129 was used in Flow cytometry/Cell sorting to study whether the full replacement of surface B cell receptors following somatic hypermutation occurs before entry to the light zone.

Tue Sep 18 00:00:00 EDT 2018

Repurposing Cytarabine for Treating Primary Effusion Lymphoma by Targeting Kaposi's Sarcoma-Associated Herpesvirus Latent and Lytic Replications.

mBio

Gruffaz M,Zhou S,Vasan K,Rushing T,Michael QL,Lu C,Jung JU,Gao SJ

Published figure using BrdU monoclonal antibody (Product # B35129) in Flow Cytometry

Tue May 08 00:00:00 EDT 2018

SIRT1 and AMPK pathways are essential for the proliferation and survival of primary effusion lymphoma cells.

The Journal of pathology

He M,Tan B,Vasan K,Yuan H,Cheng F,Ramos da Silva S,Lu C,Gao SJ

B35129 was used in flow cytometry to determine that SIRT1 is functionally required for sustaining the proliferation and survival of primary effusion lymphoma cells

Sat Jul 01 00:00:00 EDT 2017

Tenovin-6 inhibits proliferation and survival of diffuse large B-cell lymphoma cells by blocking autophagy.

Oncotarget

Yuan H,He M,Cheng F,Bai R,da Silva SR,Aguiar RC,Gao SJ

B35129 was used in Flow Cytometry to study the mechanisms by which tenovin-6 inhibits the proliferation and survival of diffuse large B-cell lymphoma cells.

Tue Feb 28 00:00:00 EST 2017

An Oncogenic Virus Promotes Cell Survival and Cellular Transformation by Suppressing Glycolysis.

PLoS pathogens

Zhu Y,Ramos da Silva S,He M,Liang Q,Lu C,Feng P,Jung JU,Gao SJ

Sun May 01 00:00:00 EDT 2016

SIRT1-mediated downregulation of p27Kip1 is essential for overcoming contact inhibition of Kaposi's sarcoma-associated herpesvirus transformed cells.

Oncotarget

He M,Yuan H,Tan B,Bai R,Kim HS,Bae S,Che L,Kim JS,Gao SJ

Published figure using BrdU monoclonal antibody (Product # B35129) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2016

BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.

PloS one

Funakoshi S,Shimizu T,Numata O,Ato M,Melchers F,Ohnishi K

B35129 was used in Flow cytometry/Cell sorting to study how CDH17 plays a role in the long-term survival of memory B cells via an 'MBC niche'.

Wed Sep 16 00:00:00 EDT 2015

Cavin-3 dictates the balance between ERK and Akt signaling.

eLife

Hernandez VJ,Weng J,Ly P,Pompey S,Dong H,Mishra L,Schwarz M,Anderson RG,Michaely P

B35129 was used in Flow cytometry/Cell sorting to elucidate the importance of cavin-3 in dictating the balance between ERK and Akt signalling.

Tue Sep 24 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine

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