Invitrogen

BrdU Monoclonal Antibody (MoBU-1), Alexa Fluor™ 647

Product Details

B35140

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 6 publications 6 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

MoBU-1

Immunogen

BrdU

Conjugate

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

Excitation/Emission Max

650/671 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2536440

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Limited access to antigen drives generation of early B cell memory while restraining the plasmablast response.

Immunity

Glaros V,Rauschmeier R,Artemov AV,Reinhardt A,Ols S,Emmanouilidi A,Gustafsson C,You Y,Mirabello C,Björklund ÅK,Perez L,King NP,Månsson R,Angeletti D,Loré K,Adameyko I,Busslinger M,Kreslavsky T

B35140 was used in Flow cytometry/Cell sorting to examine the early B cell response to T-dependent antigen in mice by single-cell RNA sequencing.

Tue Sep 14 00:00:00 EDT 2021

CD73⁺ extracellular vesicles inhibit angiogenesis through adenosine A_2B receptor signalling.

Journal of extracellular vesicles

Angioni R,Liboni C,Herkenne S,Sánchez-Rodríguez R,Borile G,Marcuzzi E,Calì B,Muraca M,Viola A

B35140 was used in Flow cytometry/Cell sorting to identify novel pathways involved in the crosstalk between endothelial and stromal cell and suggest new therapeutic strategies to target pathological angiogenesis.

Mon Sep 28 00:00:00 EDT 2020

Autoreactive, Low-Affinity T Cells Preferentially Drive Differentiation of Short-Lived Memory B Cells at the Expense of Germinal Center Maintenance.

Cell reports

Jain RW,Parham KA,Tesfagiorgis Y,Craig HC,Romanchik E,Kerfoot SM

B35140 was used in Flow cytometry/Cell sorting to characterise germinal centre kinetics and B cell fate choices in a response to the autoantigen myelin oligodendrocyte glycoprotein (MOG).

Tue Dec 18 00:00:00 EST 2018

Roles of human POLD1 and POLD3 in genome stability.

Scientific reports

Tumini E,Barroso S,-Calero CP,Aguilera A

B35140 was used in Flow cytometry/Cell sorting to demonstrate a key role of POLD1 and POLD3 in genome stability and S-phase progression.

Thu Dec 15 00:00:00 EST 2016

Intratracheal cell transfer demonstrates the profibrotic potential of resident fibroblasts in pulmonary fibrosis.

The American journal of pathology

Tsukui T,Ueha S,Shichino S,Inagaki Y,Matsushima K

B35140 was used in flow cytometry to identify the origin of activated fibroblasts

Sun Nov 01 00:00:00 EDT 2015

Antagonism of Ang-Tie2 and Dll4-Notch signaling has opposing effects on tumor endothelial cell proliferation, evidenced by a new flow cytometry method.

Laboratory investigation; a journal of technical methods and pathology

Payton M,Jun T,Wayne W,Yu D,Manoukian R,Chung G,Zhang N,Sun JR,Kaplan-Lefko P,Scully S,Van G,Radinsky R,Kendall R,Oliner J,Coxon A

B35140 was used in Flow cytometry/Cell sorting to assess the in vivo proliferative effects of angiogenesis-targeted agents on both the tumor and the associated vasculature.

Sat Nov 01 00:00:00 EDT 2014

Dual-pulse labeling using 5-ethynyl-2'-deoxyuridine (EdU) and 5-bromo-2'-deoxyuridine (BrdU) in flow cytometry.

Current protocols in cytometry

Bradford JA,Clarke ST

B35140 was used in flow cytometry to describe a dual-pulse method to label DNA using the thymidine analog 5-ethyl-2'-deoxyuridine and 5-bromo-2'-deoxyuridine.

Sat Jan 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine

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