Invitrogen

BrdU Monoclonal Antibody (MoBU-1)

BrdU Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

BrdU Antibody (B35141) in ICC/IF

Development (Cambridge, England) 2022 - Fig. 5. HH signaling regulates HBE cell proliferation. (A) Timeline for EdU, BrdU and cyclopamine administration. (B) Immunostaining for Ki67 (violet), BrdU (green) and EdU fluorescence (red) in HBE cells at the ALI after ethanol ( n =6) or 10 muM cyclopamine ( n =6) treatment at day 3. (C) Immunostaining for Ki67 (violet), BrdU (green) and EdU fluorescence (red) in HBE cells at the ALI after ethanol ( n =6... View More

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Product Details

B35141

Applications

Tested Dilution

Publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-dependent

View 7 publications 7 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Mouse

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

MoBU-1

Immunogen

BrdU

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2536441

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Reorganization of the DNA replication landscape during adipogenesis is closely linked with adipogenic gene expression.

Journal of cell science

Hayakawa T,Yamamoto A,Yoneda T,Hori S,Okochi N,Kagotani K,Okumura K,Takebayashi SI

B35141 was used in Immunocytochemistry to perform genome-wide mapping of replication timing (RT) in mouse 3T3-L1 cells before and during MCE, and identify a number of chromosomal regions shifting toward either earlier or later replication through two rounds of replication.

Sun Jan 15 00:00:00 EST 2023

An essential function for autocrine hedgehog signaling in epithelial proliferation and differentiation in the trachea.

Development (Cambridge, England)

Yin W,Liontos A,Koepke J,Ghoul M,Mazzocchi L,Liu X,Lu C,Wu H,Fysikopoulos A,Sountoulidis A,Seeger W,Ruppert C,Günther A,Stainier DYR,Samakovlis C

B35141 was used in Immunocytochemistry to investigate the effects of hedgehog signalling on epithelial tracheal cells.

Tue Feb 01 00:00:00 EST 2022

An essential function for autocrine hedgehog signaling in epithelial proliferation and differentiation in the trachea.

Development (Cambridge, England)

Yin W,Liontos A,Koepke J,Ghoul M,Mazzocchi L,Liu X,Lu C,Wu H,Fysikopoulos A,Sountoulidis A,Seeger W,Ruppert C,Günther A,Stainier DYR,Samakovlis C

B35141 was used in Immunocytochemistry to investigate the effects of hedgehog signalling on epithelial tracheal cells.

Tue Feb 01 00:00:00 EST 2022

Alzheimer's disease like neuropathology in Down syndrome cortical organoids.

Frontiers in cellular neuroscience

Zhao HH,Haddad GG

B35141 was used in flow cytometry to use induced pluripotent stem cell (iPSC) and iPSC-derived 3D cortical organoids to model Alzheimer's disease in Down syndrome and explore the earliest cellular and molecular changes during DS fetal brain development.

Tue Jan 03 00:00:00 EST 2023

The 6-4 photoproduct is the trigger of UV-induced replication blockage and ATR activation.

Proceedings of the National Academy of Sciences of the United States of America

Hung KF,Sidorova JM,Nghiem P,Kawasumi M

B35141 was used in Flow cytometry/Cell sorting to couple multiparameter flow cytometry with lesion-specific photolyases that eliminate either CPDs or 6-4PPs and determined their respective contributions to DNA damage responses.

Tue Jun 09 00:00:00 EDT 2020

The 6-4 photoproduct is the trigger of UV-induced replication blockage and ATR activation.

Proceedings of the National Academy of Sciences of the United States of America

Hung KF,Sidorova JM,Nghiem P,Kawasumi M

Tue Jun 09 00:00:00 EDT 2020

Multiplex Cell Fate Tracking by Flow Cytometry.

Methods and protocols

Rodríguez-Martínez M,Hills SA,Diffley JFX,Svejstrup JQ

B35141 was used in Flow cytometry/Cell sorting to describe a method to analyze cell proliferation and cell cycle progression by double staining with thymidine analogues in combination with fluorescent cell barcoding, which allows one to multiplex the study and reduces the variability due to individual sample staining, reducing also the cost of the experiment.

Fri Jul 17 00:00:00 EDT 2020

Multiplex Cell Fate Tracking by Flow Cytometry.

Methods and protocols

Rodríguez-Martínez M,Hills SA,Diffley JFX,Svejstrup JQ

Fri Jul 17 00:00:00 EDT 2020

Adaptation to Endoplasmic Reticulum Stress Enhances Resistance of Oral Cancer Cells to Cisplatin by Up-Regulating Polymerase η and Increasing DNA Repair Efficiency.

International journal of molecular sciences

Chen CY,Kawasumi M,Lan TY,Poon CL,Lin YS,Wu PJ,Chen YC,Chen BH,Wu CH,Lo JF,Weng RR,Sun YC,Hung KF

B35141 was used in Flow cytometry/Cell sorting to uncover that adaptation to ER stress enhances DNA repair and damage tolerance, with which stressed cells gain resistance to chemotherapeutics.

Thu Dec 31 00:00:00 EST 2020

Cell surface glycoengineering improves selectin-mediated adhesion of mesenchymal stem cells (MSCs) and cardiosphere-derived cells (CDCs): Pilot validation in porcine ischemia-reperfusion model.

Biomaterials

Lo CY,Weil BR,Palka BA,Momeni A,Canty JM,Neelamegham S

B35141 was used in flow cytometry to discuss and investigate methods of cellular glycoengineering.

Fri Jan 01 00:00:00 EST 2016

Cyclin G2 promotes cell cycle arrest in breast cancer cells responding to fulvestrant and metformin and correlates with patient survival.

Cell cycle (Georgetown, Tex.)

Zimmermann M,Arachchige-Don AP,Donaldson MS,Patriarchi T,Horne MC

Published figure using BrdU monoclonal antibody (Product # B35141) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

The Periodontal Pathogen Porphyromonas gingivalis Preferentially Interacts with Oral Epithelial Cells in S Phase of the Cell Cycle.

Infection and immunity

Al-Taweel FB,Douglas CWI,Whawell SA

B35141 was used in immunocytochemistry to investigate the effect of oral keratinocyte cell cycle on Porphyromonas gingivalis infection

Fri Jul 01 00:00:00 EDT 2016

MERIT40 cooperates with BRCA2 to resolve DNA interstrand cross-links.

Genes & development

Jiang Q,Paramasivam M,Aressy B,Wu J,Bellani M,Tong W,Seidman MM,Greenberg RA

B35141 was used in flow cytometry to study resolution of DNA interstrand cross-links by cooperation of BRCA2 and MERIT40

Tue Sep 15 00:00:00 EDT 2015

Tumor suppressor and deubiquitinase BAP1 promotes DNA double-strand break repair.

Proceedings of the National Academy of Sciences of the United States of America

Yu H,Pak H,Hammond-Martel I,Ghram M,Rodrigue A,Daou S,Barbour H,Corbeil L,Hébert J,Drobetsky E,Masson JY,Di Noia JM,Affar el B

B35141 was used in flow cytometry to determine deubiquitinases required to assemble homologous recombination factors BRCA1 and RAD51

Tue Jan 07 00:00:00 EST 2014

Notch3 signaling gates cell cycle entry and limits neural stem cell amplification in the adult pallium.

Development (Cambridge, England)

Alunni A,Krecsmarik M,Bosco A,Galant S,Pan L,Moens CB,Bally-Cuif L

B35141 was used in immunohistochemistry (free floating) to examine the role of Notch3 in neuronal stem cells.

Thu Aug 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine

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Performance Guarantee

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BrdU Monoclonal Antibody (MoBU-1)

BrdU Antibody (B35141) in ICC/IF

Product Details

B35141

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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