Invitrogen

CD117 (c-Kit) Monoclonal Antibody (104D2), PE-Cyanine7, eBioscience™

CD117 (c-Kit) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 6

CD117 (c-Kit) Antibody (25-1178-42) in Flow

Staining of normal human peripheral blood cells with the Human Hematopoietic Lineage APC Cocktail (Product # 22-7776-72), Anti-Human CD34 FITC (Product # 11-0349-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD117 (c-kit) PE-Cyanine7 (right). Lineage negative/low cells were used for analysis.

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Product Details

25-1178-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

104D2

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10718535

Product Specific Information

Description: The 104D2 monoclonal antibody reacts with human CD117, also known as c-Kit, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells.

Applications Reported: This 104D2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 104D2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression.

International journal of molecular sciences

Li N,Wang Y,Wang A,Zhang J,Jia C,Yu C,Song Z,Wang S,Liu L,Yi J,Bao Y,Huang Y,Sun L

25-1178-42 was used in Flow cytometry/Cell sorting to suggest BC as one of the small molecule candidates to stimulate HSPC expansion both in vitro and in vivo when needed in either physiologic or pathologic conditions, but also supports STS1/STS2 as potential therapeutic drug targets for HSPC expansion and hematopoietic injury recovery.

Fri Feb 03 00:00:00 EST 2023

STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression.

International journal of molecular sciences

Li N,Wang Y,Wang A,Zhang J,Jia C,Yu C,Song Z,Wang S,Liu L,Yi J,Bao Y,Huang Y,Sun L

Fri Feb 03 00:00:00 EST 2023

KIT D816V Mast Cells Derived from Induced Pluripotent Stem Cells Recapitulate Systemic Mastocytosis Transcriptional Profile.

International journal of molecular sciences

de Toledo MAS,Fu X,Maié T,Buhl EM,Götz K,Schmitz S,Kaiser A,Boor P,Braunschweig T,Chatain N,Costa IG,Brümmendorf TH,Koschmieder S,Zenke M

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1178-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2023

Airway and Lung Organoids from Human-Induced Pluripotent Stem Cells Can Be Used to Assess CFTR Conductance.

International journal of molecular sciences

Demchenko A,Kondrateva E,Tabakov V,Efremova A,Salikhova D,Bukharova T,Goldshtein D,Balyasin M,Bulatenko N,Amelina E,Lavrov A,Smirnikhina S

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1178-42) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2023

R274X-mutated Phf6 increased the self-renewal and skewed T cell differentiation of hematopoietic stem cells.

iScience

Lan Y,Yuan S,Guo T,Hou S,Zhao F,Yang W,Cao Y,Chu Y,Jiang E,Yuan W,Wang X

25-1178-42 was used in Flow Cytometry to demonstrate that Phf6R274X plays a critical role in fine-tuning T cells and HSC homeostasis.

Fri Jun 16 00:00:00 EDT 2023

Human oral mucosa cell atlas reveals a stromal-neutrophil axis regulating tissue immunity.

Cell

Williams DW,Greenwell-Wild T,Brenchley L,Dutzan N,Overmiller A,Sawaya AP,Webb S,Martin D,Hajishengallis G,Divaris K,Morasso M,Haniffa M,Moutsopoulos NM

25-1178-42 was used in Flow cytometry/Cell sorting to link exaggerated stromal cell responsiveness with enhanced neutrophil and leukocyte infiltration in periodontitis.

Thu Jul 22 00:00:00 EDT 2021

Coordinated alterations in RNA splicing and epigenetic regulation drive leukaemogenesis.

Nature

Yoshimi A,Lin KT,Wiseman DH,Rahman MA,Pastore A,Wang B,Lee SC,Micol JB,Zhang XJ,de Botton S,Penard-Lacronique V,Stein EM,Cho H,Miles RE,Inoue D,Albrecht TR,Somervaille TCP,Batta K,Amaral F,Simeoni F,Wilks DP,Cargo C,Intlekofer AM,Levine RL,Dvinge H,Bradley RK,Wagner EJ,Krainer AR,Abdel-Wahab O

25-1178 was used in Flow cytometry/Cell sorting to study the mutations in IDH2 and SRSF2 that together promote leukaemogenesis through coordinated effects on the epigenome and RNA splicing.

Tue Oct 01 00:00:00 EDT 2019

Tracking of epigenetic changes during hematopoietic differentiation of induced pluripotent stem cells.

Clinical epigenetics

Cypris O,Frobel J,Rai S,Franzen J,Sontag S,Goetzke R,Szymanski de Toledo MA,Zenke M,Wagner W

25-1178-42 was used in Flow Cytometry to compare genome-wide DNA methylation (DNAm) patterns of iHPCs with various different hematopoietic subsets.

Mon Feb 04 00:00:00 EST 2019

CD62L Is a Functional and Phenotypic Marker for Circulating Innate Lymphoid Cell Precursors.

Journal of immunology (Baltimore, Md. : 1950)

Bar-Ephraim YE,Koning JJ,Burniol Ruiz E,Konijn T,Mourits VP,Lakeman KA,Boon L,Bögels M,van Maanen JP,Den Haan JMM,van Egmond M,Bouma G,Reijmers RM,Mebius RE

25-1178-42 was used in Flow Cytometry to demonstrate that CD62L not only serves as a functional marker of ILCP, but has potential to be used in the clinic as a diagnostic marker in inflammatory disorders.

Tue Jan 01 00:00:00 EST 2019

Chemically-Defined, Xeno-Free, Scalable Production of hPSC-Derived Definitive Endoderm Aggregates with Multi-Lineage Differentiation Potential.

Cells

Sahabian A,Sgodda M,Naujok O,Dettmer R,Dahlmann J,Manstein F,Cantz T,Zweigerdt R,Martin U,Olmer R

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1178-42) in Flow Cytometry

Wed Dec 04 00:00:00 EST 2019

Modelling IRF8 Deficient Human Hematopoiesis and Dendritic Cell Development with Engineered iPS Cells.

Stem cells (Dayton, Ohio)

Sontag S,Förster M,Qin J,Wanek P,Mitzka S,Schüler HM,Koschmieder S,Rose-John S,Seré K,Zenke M

25-1178 was used in Flow cytometry/Cell sorting to engineer a human IRF8 knockout model that allows studying molecular mechanisms of human immunodeficiencies in vitro, including the pathophysiology of IRF8 deficient DC.

Sat Apr 01 00:00:00 EDT 2017

Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

Nature communications

Huang NJ,Pishesha N,Mukherjee J,Zhang S,Deshycka R,Sudaryo V,Dong M,Shoemaker CB,Lodish HF

25-1178 was used in Flow cytometry/Cell sorting to engineer mouse and human red blood cells expressing surface single-domain antibodies against botulinum neurotoxin A, conferring long lasting protection.

Mon Sep 04 00:00:00 EDT 2017

Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells.

Oncoimmunology

Seebauer CT,Brunner S,Glockzin G,Piso P,Ruemmele P,Schlitt HJ,Geissler EK,Fichtner-Feigl S,Kesselring R

25-1178 was used in Flow cytometry/Cell sorting to characterise the immunological tumour microenvironment in proliferation, senescence and neovascularization.

Tue Jan 12 00:00:00 EST 2021

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1178-42) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

CD52 is a molecular target in advanced systemic mastocytosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hoermann G,Blatt K,Greiner G,Putz EM,Berger A,Herrmann H,Cerny-Reiterer S,Gleixner KV,Walz C,Hoetzenecker K,Müllauer L,Reiter A,Sotlar K,Sexl V,Valent P,Mayerhofer M

25-1178 was used in Flow cytometry/Cell sorting to show that CD52 is a novel marker and potential therapeutic target in neoplastic mast cells in patients with advanced systemic mastocytosis.

Fri Aug 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD117; ckit; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Gene Aliases: C-Kit; CD117; KIT; PBT; SCFR

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UniProt ID: (Human) P10721

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Entrez Gene ID: (Human) 3815

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Function(s)

protease binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity stem cell factor receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding cytokine binding protein homodimerization activity metal ion binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD117 (c-Kit) Monoclonal Antibody (104D2), PE-Cyanine7, eBioscience™

CD117 (c-Kit) Antibody (25-1178-42) in Flow

Product Details

25-1178-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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