Invitrogen

CD117 (c-Kit) Monoclonal Antibody (2B8), PE-Cyanine5, eBioscience™

CD117 (c-Kit) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 73

CD117 (c-Kit) Antibody (15-1171-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2b Isotype Control PE-Cyanine5 (Product # 15-4031-82) (left) or 0.06 µg of Anti-Mouse CD117 (c-Kit) PE-Cyanine5 (right).Total viable cells were used for analysis.

Product Image Gallery

Product Details

15-1171-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 72 publications 72 publications

Product Specifications

Species Reactivity

Mouse, Pig

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2B8

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468787

Product Specific Information

Description: The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor, and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145-kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1 low) and negative for lineage markers. The interaction of the mouse c-Kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells.

Applications Reported: The 2B8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2B8 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Calcineurin Aα Contributes to IgE-Dependent Mast-Cell Mediator Secretion in Allergic Inflammation.

Journal of innate immunity

Leong E,Pang Z,Stadnyk AW,Lin TJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Jul 07 00:00:00 EDT 2022

Gastruloids as in vitro models of embryonic blood development with spatial and temporal resolution.

Scientific reports

Rossi G,Giger S,Hübscher T,Lutolf MP

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Aug 04 00:00:00 EDT 2022

Gastruloids as in vitro models of embryonic blood development with spatial and temporal resolution.

Scientific reports

Rossi G,Giger S,Hübscher T,Lutolf MP

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Aug 04 00:00:00 EDT 2022

Specific overexpression of SIRT1 in mesenchymal stem cells rescues hematopoiesis niche in BMI1 knockout mice through promoting CXCL12 expression.

International journal of biological sciences

Li J,Li X,Sun W,Zhang J,Yan Q,Wu J,Jin J,Lu R,Miao D

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2022

Preventive Effects of a Human Hematopoietic Mesenchymal Stem Cell (hHMSC) Therapy in Ovalbumin-Induced Food Allergy.

Biomedicines

Lee DG,Lee YJ,Park SH,Park HR,Kang H,Kim JE

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Mon Feb 21 00:00:00 EST 2022

Inaccessible LCG Promoters Act as Safeguards to Restrict T Cell Development to Appropriate Notch Signaling Environments.

Stem cell reports

Furuyama S,Wu QV,Varnum-Finney B,Sandstrom R,Meuleman W,Stamatoyannopoulos JA,Bernstein ID

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Inaccessible LCG Promoters Act as Safeguards to Restrict T Cell Development to Appropriate Notch Signaling Environments.

Stem cell reports

Furuyama S,Wu QV,Varnum-Finney B,Sandstrom R,Meuleman W,Stamatoyannopoulos JA,Bernstein ID

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Mitochondrial transcription factor A in RORγt⁺ lymphocytes regulate small intestine homeostasis and metabolism.

Nature communications

Fu Z,Dean JW,Xiong L,Dougherty MW,Oliff KN,Chen ZE,Jobin C,Garrett TJ,Zhou L

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Jul 22 00:00:00 EDT 2021

SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis.

The Journal of biological chemistry

Cardenas RA,Gonzalez R,Sanchez E,Ramos MA,Cardenas EI,Rodarte AI,Alcazar-Felix RJ,Isaza A,Burns AR,Heidelberger R,Adachi R

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

mMCP7, a Mouse Ortholog of δ Tryptase, Mediates Pelvic Tactile Allodynia in a Model of Chronic Pelvic Pain.

Frontiers in pain research (Lausanne, Switzerland)

Pattabiraman G,Liu Z,Paul M,Schaeffer AJ,Thumbikat P

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

Analysis of Radiation-Induced Changes in Cell Cycle and DNA Damage of Murine Hematopoietic Stem Cells by Multi-Color Flow Cytometry.

Current protocols

Majumdar D,Pietras EM,Pawar SA

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

XIAOPI formula inhibits the pre-metastatic niche formation in breast cancer via suppressing TAMs/CXCL1 signaling.

Cell communication and signaling : CCS

Zheng Y,Wang N,Wang S,Yang B,Situ H,Zhong L,Lin Y,Wang Z

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Mar 26 00:00:00 EDT 2020

USP15 Deubiquitinase Safeguards Hematopoiesis and Genome Integrity in Hematopoietic Stem Cells and Leukemia Cells.

Cell reports

van den Berk P,Lancini C,Company C,Serresi M,Sanchez-Bailon MP,Hulsman D,Pritchard C,Song JY,Schmitt MJ,Tanger E,Popp O,Mertins P,Huijbers IJ,Jacobs H,van Lohuizen M,Gargiulo G,Citterio E

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Tue Dec 29 00:00:00 EST 2020

Authentication of Primary Murine Cell Lines by a Microfluidics-Based Lab-On-Chip System.

Biomedicines

Hong Y,Singh N,Bamopoulos S,Gjerga E,Schmalbrock LK,Balabanian K,Schick M,Keller U,Wirth M

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Wed Dec 09 00:00:00 EST 2020

Type I Interferon α/β Receptor-Mediated Signaling Negatively Regulates Antiviral Cytokine Responses in Murine Bone-Marrow-Derived Mast Cells and Protects the Cells from Virus-Induced Cell Death.

International journal of molecular sciences

Darzianiazizi M,Mehrani Y,Chan L,Mould RC,Kulkarni RR,Sharif S,Bridle BW,Karimi K

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

Remodeling of Bone Marrow Hematopoietic Stem Cell Niches Promotes Myeloid Cell Expansion during Premature or Physiological Aging.

Cell stem cell

Ho YH,Del Toro R,Rivera-Torres J,Rak J,Korn C,García-García A,Macías D,González-Gómez C,Del Monte A,Wittner M,Waller AK,Foster HR,López-Otín C,Johnson RS,Nerlov C,Ghevaert C,Vainchenker W,Louache F,Andrés V,Méndez-Ferrer S

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Sep 05 00:00:00 EDT 2019

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.

Cell reports

Balzano M,De Grandis M,Vu Manh TP,Chasson L,Bardin F,Farina A,Sergé A,Bidaut G,Charbord P,Hérault L,Bailly AL,Cartier-Michaud A,Boned A,Dalod M,Duprez E,Genever P,Coles M,Bajenoff M,Xerri L,Aurrand-Lions M,Schiff C,Mancini SJC

15-1171 was used in Flow cytometry/Cell sorting to study pro-B cells in their support of stroll cells and their interaction network allowing their retention.

Tue Mar 19 00:00:00 EDT 2019

Defective germline reprogramming rewires the spermatogonial transcriptome.

Nature structural & molecular biology

Vasiliauskaitė L,Berrens RV,Ivanova I,Carrieri C,Reik W,Enright AJ,O'Carroll D

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

JAK2 is dispensable for maintenance of JAK2 mutant B-cell acute lymphoblastic leukemias.

Genes & development

Kim SK,Knight DA,Jones LR,Vervoort S,Ng AP,Seymour JF,Bradner JE,Waibel M,Kats L,Johnstone RW

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Antiasthmatic Effects of Sanglong Pingchuan Decoction through Inducing a Balanced Th1/Th2 Immune Response.

Evidence-based complementary and alternative medicine : eCAM

Zhu B,Dong J,Gao X,He Y,Sun H

15-1171 was used in Flow cytometry/Cell sorting to investigate the antiasthmatic effects of Sanglong pingchuan decoction and to explore its mechanisms of action.

Fri Mar 18 00:00:00 EDT 2022

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Wed Jun 20 00:00:00 EDT 2018

A stochastic epigenetic switch controls the dynamics of T-cell lineage commitment.

eLife

Ng KK,Yui MA,Mehta A,Siu S,Irwin B,Pease S,Hirose S,Elowitz MB,Rothenberg EV,Kueh HY

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice.

eLife

Schmidt K,Zhang Q,Tasdogan A,Petzold A,Dahl A,Arneth BM,Slany R,Fehling HJ,Kranz A,Stewart AF,Anastassiadis K

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Tue Jun 19 00:00:00 EDT 2018

Generation of Vascular Endothelial Cells and Hematopoietic Cells by Blastocyst Complementation.

Stem cell reports

Hamanaka S,Umino A,Sato H,Hayama T,Yanagida A,Mizuno N,Kobayashi T,Kasai M,Suchy FP,Yamazaki S,Masaki H,Yamaguchi T,Nakauchi H

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Tue Oct 09 00:00:00 EDT 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Tue Mar 20 00:00:00 EDT 2018

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

Positive and Negative Regulatory Roles of C-Terminal Src Kinase (CSK) in FcεRI-Mediated Mast Cell Activation, Independent of the Transmembrane Adaptor PAG/CSK-Binding Protein.

Frontiers in immunology

Potuckova L,Draberova L,Halova I,Paulenda T,Draber P

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Mon Oct 07 00:00:00 EDT 2019

Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.

Nature

Hérault A,Binnewies M,Leong S,Calero-Nieto FJ,Zhang SY,Kang YA,Wang X,Pietras EM,Chu SH,Barry-Holson K,Armstrong S,Göttgens B,Passegué E

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Apr 06 00:00:00 EDT 2017

GATA Factor-Regulated Samd14 Enhancer Confers Red Blood Cell Regeneration and Survival in Severe Anemia.

Developmental cell

Hewitt KJ,Katsumura KR,Matson DR,Devadas P,Tanimura N,Hebert AS,Coon JJ,Kim JS,Dewey CN,Keles S,Hao S,Paulson RF,Bresnick EH

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Mon Aug 07 00:00:00 EDT 2017

Targeted genome editing restores T cell differentiation in a humanized X-SCID pluripotent stem cell disease model.

Scientific reports

Alzubi J,Pallant C,Mussolino C,Howe SJ,Thrasher AJ,Cathomen T

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Fri Sep 29 00:00:00 EDT 2017

Sorting protein VPS33B regulates exosomal autocrine signaling to mediate hematopoiesis and leukemogenesis.

The Journal of clinical investigation

Gu H,Chen C,Hao X,Wang C,Zhang X,Li Z,Shao H,Zeng H,Yu Z,Xie L,Xia F,Zhang F,Liu X,Zhang Y,Jiang H,Zhu J,Wan J,Wang C,Weng W,Xie J,Tao M,Zhang CC,Liu J,Chen GQ,Zheng J

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

PAFAH1B1 and the lncRNA NONHSAT073641 maintain an angiogenic phenotype in human endothelial cells.

Acta physiologica (Oxford, England)

Josipovic I,Fork C,Preussner J,Prior KK,Iloska D,Vasconez AE,Labocha S,Angioni C,Thomas D,Ferreirós N,Looso M,Pullamsetti SS,Geisslinger G,Steinhilber D,Brandes RP,Leisegang MS

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Physiological and hypoxic oxygen concentration differentially regulates human c-Kit+ cardiac stem cell proliferation and migration.

American journal of physiology. Heart and circulatory physiology

Bellio MA,Rodrigues CO,Landin AM,Hatzistergos KE,Kuznetsov J,Florea V,Valasaki K,Khan A,Hare JM,Schulman IH

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Arrested Hematopoiesis and Vascular Relaxation Defects in Mice with a Mutation in Dhfr.

Molecular and cellular biology

Thoms JA,Knezevic K,Liu JJ,Glaros EN,Thai T,Qiao Q,Campbell H,Packham D,Huang Y,Papathanasiou P,Tunningley R,Whittle B,Yeung AW,Chandrakanthan V,Hesson L,Chen V,Wong JW,Purton LE,Ward RL,Thomas SR,Pimanda JE

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2016

New Regulatory Roles of Galectin-3 in High-Affinity IgE Receptor Signaling.

Molecular and cellular biology

Bambouskova M,Polakovicova I,Halova I,Goel G,Draberova L,Bugajev V,Doan A,Utekal P,Gardet A,Xavier RJ,Draber P

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

The miR-17∼92 cluster contributes to MLL leukemia through the repression of MEIS1 competitor PKNOX1.

Leukemia research

Mian YA,Zeleznik-Le NJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

PRDM14 promotes RAG-dependent Notch1 driver mutations in mouse T-ALL.

Biology open

Carofino BL,Ayanga B,Tracey LJ,Brooke-Bisschop T,Justice MJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Sun May 15 00:00:00 EDT 2016

Transcriptional regulation of the proto-oncogene Zfp521 by SPI1 (PU.1) and HOXC13.

Genesis (New York, N.Y. : 2000)

Yu M,Al-Dallal S,Al-Haj L,Panjwani S,McCartney AS,Edwards SM,Manjunath P,Walker C,Awgulewitsch A,Hentges KE

15-1171 was used in Flow cytometry/Cell sorting to study the regulatory factors controlling the mouse zinc-finger gene Zfp521; a B-cell proto-oncogene.

Sat Oct 01 00:00:00 EDT 2016

BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo.

Stem cell reports

Crisan M,Solaimani Kartalaei P,Neagu A,Karkanpouna S,Yamada-Inagawa T,Purini C,Vink CS,van der Linden R,van Ijcken W,Chuva de Sousa Lopes SM,Monteiro R,Mummery C,Dzierzak E

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2016

LTβR controls thymic portal endothelial cells for haematopoietic progenitor cell homing and T-cell regeneration.

Nature communications

Shi Y,Wu W,Chai Q,Li Q,Hou Y,Xia H,Ren B,Xu H,Guo X,Jin C,Lv M,Wang Z,Fu YX,Zhu M

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Fri Aug 05 00:00:00 EDT 2016

Deep imaging of bone marrow shows non-dividing stem cells are mainly perisinusoidal.

Nature

Acar M,Kocherlakota KS,Murphy MM,Peyer JG,Oguro H,Inra CN,Jaiyeola C,Zhao Z,Luby-Phelps K,Morrison SJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

Mast cells mediate malignant pleural effusion formation.

The Journal of clinical investigation

Giannou AD,Marazioti A,Spella M,Kanellakis NI,Apostolopoulou H,Psallidas I,Prijovich ZM,Vreka M,Zazara DE,Lilis I,Papaleonidopoulos V,Kairi CA,Patmanidi AL,Giopanou I,Spiropoulou N,Harokopos V,Aidinis V,Spyratos D,Teliousi S,Papadaki H,Taraviras S,Snyder LA,Eickelberg O,Kardamakis D,Iwakura Y,Feyerabend TB,Rodewald HR,Kalomenidis I,Blackwell TS,Agalioti T,Stathopoulos GT

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Cis-regulatory mechanisms governing stem and progenitor cell transitions.

Science advances

Johnson KD,Kong G,Gao X,Chang YI,Hewitt KJ,Sanalkumar R,Prathibha R,Ranheim EA,Dewey CN,Zhang J,Bresnick EH

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Merlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation.

PloS one

Zoch A,Mayerl S,Schulz A,Greither T,Frappart L,Rübsam J,Heuer H,Giovannini M,Morrison H

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Mon May 23 00:00:00 EDT 2016

Abi3bp regulates cardiac progenitor cell proliferation and differentiation.

Circulation research

Hodgkinson CP,Gomez JA,Payne AJ,Zhang L,Wang X,Dal-Pra S,Pratt RE,Dzau VJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Fri Dec 05 00:00:00 EST 2014

WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

The Journal of experimental medicine

Xia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Mon Sep 22 00:00:00 EDT 2014

A crucial role for the ubiquitously expressed transcription factor Sp1 at early stages of hematopoietic specification.

Development (Cambridge, England)

Gilmour J,Assi SA,Jaegle U,Kulu D,van de Werken H,Clarke D,Westhead DR,Philipsen S,Bonifer C

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Sun Jun 01 00:00:00 EDT 2014

Identification of hematopoietic-specific regulatory elements from the CD45 gene and use for lentiviral tracking of transplanted cells.

Experimental hematology

Duong KL,Das S,Yu S,Barr JY,Jena S,Kim E,Zavazava N,Colgan JD,Xue HH,Levasseur DN

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2014

F-box protein FBXL16 binds PP2A-B55α and regulates differentiation of embryonic stem cells along the FLK1+ lineage.

Molecular & cellular proteomics : MCP

Honarpour N,Rose CM,Brumbaugh J,Anderson J,Graham RL,Sweredoski MJ,Hess S,Coon JJ,Deshaies RJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Sat Mar 01 00:00:00 EST 2014

miR-126 and miR-126* repress recruitment of mesenchymal stem cells and inflammatory monocytes to inhibit breast cancer metastasis.

Nature cell biology

Zhang Y,Yang P,Sun T,Li D,Xu X,Rui Y,Li C,Chong M,Ibrahim T,Mercatali L,Amadori D,Lu X,Xie D,Li QJ,Wang XF

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

Pten loss in the bone marrow leads to G-CSF-mediated HSC mobilization.

The Journal of experimental medicine

Tesio M,Oser GM,Baccelli I,Blanco-Bose W,Wu H,Göthert JR,Kogan SC,Trumpp A

15-1171 was used in Flow cytometry/Cell sorting to investigate the effect of PTEN deletion in bone marrow, showing that it leads to G-CSF-mediated hematopoietic stem cell mobilisation.

Mon Oct 21 00:00:00 EDT 2013

PLC-γ and PI3K link cytokines to ERK activation in hematopoietic cells with normal and oncogenic Kras.

Science signaling

Diaz-Flores E,Goldschmidt H,Depeille P,Ng V,Akutagawa J,Krisman K,Crone M,Burgess MR,Williams O,Houseman B,Shokat K,Sampath D,Bollag G,Roose JP,Braun BS,Shannon K

15-1171 was used in Flow cytometry/Cell sorting to demonstrate cooperativity between PLC-γ and PI3K in linking GM-CSF receptor activation to Ras and ERK signaling in primary hematopoietic cells.

Tue Dec 03 00:00:00 EST 2013

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

A non-redundant function of cyclin E1 in hematopoietic stem cells.

Cell cycle (Georgetown, Tex.)

Campaner S,Viale A,De Fazio S,Doni M,De Franco F,D'Artista L,Sardella D,Pelicci PG,Amati B

15-1171 was used in Flow cytometry/Cell sorting to support a role for cyclin E1 in controlling the exit from quiescence in hematopoietic stem cells.

Sun Dec 01 00:00:00 EST 2013

Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo.

PloS one

Kavanagh DP,Yemm AI,Zhao Y,Frampton J,Kalia N

15-1171 was used in Flow cytometry/Cell sorting to study the mechanisms of haematopoietic stem cell adhesion to gut and muscle and the relationship between adhesion and leukocyte recruitment.

Tue Sep 24 00:00:00 EDT 2013

Ionizing radiation-induced expression of INK4a/ARF in murine bone marrow-derived stromal cell populations interferes with bone marrow homeostasis.

Blood

Carbonneau CL,Despars G,Rojas-Sutterlin S,Fortin A,Le O,Hoang T,Beauséjour CM

15-1171 was used in Flow cytometry/Cell sorting to investigate the effect of ionizing radiation on murine bone marrow-derived stromal cell populations, and overall bone marrow homeostasis.

Thu Jan 19 00:00:00 EST 2012

Transcription factor E3, a major regulator of mast cell-mediated allergic response.

The Journal of allergy and clinical immunology

Yagil Z,Hadad Erlich T,Ofir-Birin Y,Tshori S,Kay G,Yekhtin Z,Fisher DE,Cheng C,Wong WS,Hartmann K,Razin E,Nechushtan H

15-1171 was used in Flow cytometry/Cell sorting to explore the role played by TFE3 in mast cell function.

Tue May 01 00:00:00 EDT 2012

CTC1 deletion results in defective telomere replication, leading to catastrophic telomere loss and stem cell exhaustion.

The EMBO journal

Gu P,Min JN,Wang Y,Huang C,Peng T,Chai W,Chang S

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Wed May 16 00:00:00 EDT 2012

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Partial characterization of the Sox2+ cell population in an adult murine model of digit amputation.

Tissue engineering. Part A

Agrawal V,Siu BF,Chao H,Hirschi KK,Raborn E,Johnson SA,Tottey S,Hurley KB,Medberry CJ,Badylak SF

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2012

RNAi screen identifies Brd4 as a therapeutic target in acute myeloid leukaemia.

Nature

Zuber J,Shi J,Wang E,Rappaport AR,Herrmann H,Sison EA,Magoon D,Qi J,Blatt K,Wunderlich M,Taylor MJ,Johns C,Chicas A,Mulloy JC,Kogan SC,Brown P,Valent P,Bradner JE,Lowe SW,Vakoc CR

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Wed Aug 03 00:00:00 EDT 2011

Essential roles for Pot1b in HSC self-renewal and survival.

Blood

Wang Y,Shen MF,Chang S

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment.

Blood

Cook BD,Liu S,Evans T

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Jun 16 00:00:00 EDT 2011

Intramuscular VEGF activates an SDF1-dependent progenitor cell cascade and an SDF1-independent muscle paracrine cascade for cardiac repair.

American journal of physiology. Heart and circulatory physiology

Zisa D,Shabbir A,Mastri M,Taylor T,Aleksic I,McDaniel M,Suzuki G,Lee T

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Sox3 functions in a cell-autonomous manner to regulate spermatogonial differentiation in mice.

Endocrinology

Laronda MM,Jameson JL

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2011

A high omega-3 fatty acid diet has different effects on early and late stage myeloid progenitors.

Lipids

Varney ME,Buchanan JT,Dementieva Y,Hardman WE,Sollars VE

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2011

Activating transcription factor 4 regulates osteoclast differentiation in mice.

The Journal of clinical investigation

Cao H,Yu S,Yao Z,Galson DL,Jiang Y,Zhang X,Fan J,Lu B,Guan Y,Luo M,Lai Y,Zhu Y,Kurihara N,Patrene K,Roodman GD,Xiao G

15-1171 was used in Flow cytometry/Cell sorting to illustrate the potential of ATF4 as a therapeutic target for treating bone diseases associated with increased OCL activity.

Sun Aug 01 00:00:00 EDT 2010

CBFbeta is critical for AML1-ETO and TEL-AML1 activity.

Blood

Roudaia L,Cheney MD,Manuylova E,Chen W,Morrow M,Park S,Lee CT,Kaur P,Williams O,Bushweller JH,Speck NA

15-1171 was used in Flow cytometry/Cell sorting to validate the AML1-ETO and TEL-AML1 chimeric protein Runt domain/CBFbeta interaction as a therapeutic target in core binding factor leukemias.

Thu Mar 26 00:00:00 EDT 2009

Heart failure therapy mediated by the trophic activities of bone marrow mesenchymal stem cells: a noninvasive therapeutic regimen.

American journal of physiology. Heart and circulatory physiology

Shabbir A,Zisa D,Suzuki G,Lee T

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2009

E47 controls the developmental integrity and cell cycle quiescence of multipotential hematopoietic progenitors.

Journal of immunology (Baltimore, Md. : 1950)

Yang Q,Kardava L,St Leger A,Martincic K,Varnum-Finney B,Bernstein ID,Milcarek C,Borghesi L

15-1171 was used in Flow cytometry/Cell sorting to show E47 controls the developmental integrity and cell cycle quiescence of hematopoietic progenitors.

Sat Nov 01 00:00:00 EDT 2008

Gfi1b:green fluorescent protein knock-in mice reveal a dynamic expression pattern of Gfi1b during hematopoiesis that is largely complementary to Gfi1.

Blood

Vassen L,Okayama T,Möröy T

15-1171 was used in Flow cytometry/Cell sorting to observe the dynamic and complementary expression pattern of Gfi1b and Gfi1 during hematopoiesis in a GFP knock-in reporter mouse strain.

Thu Mar 15 00:00:00 EDT 2007

K-RasG12D expression induces hyperproliferation and aberrant signaling in primary hematopoietic stem/progenitor cells.

Blood

Van Meter ME,Díaz-Flores E,Archard JA,Passegué E,Irish JM,Kotecha N,Nolan GP,Shannon K,Braun BS

15-1171 was used in Flow cytometry/Cell sorting to investigate the effects of an oncogenic KrasG12D allele on phosphorylated signaling molecules in primary c-kitlin/low hematopoietic progenitor cells.

Tue May 01 00:00:00 EDT 2007

Essential role of phospholipase C gamma 2 in early B-cell development and Myc-mediated lymphomagenesis.

Molecular and cellular biology

Wen R,Chen Y,Bai L,Fu G,Schuman J,Dai X,Zeng H,Yang C,Stephan RP,Cleveland JL,Wang D

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 15-1171-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: belly-spot; c-kit; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit

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Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; MGF; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W

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UniProt ID: (Pig) Q2HWD6, (Mouse) P05532

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Entrez Gene ID: (Pig) 396810, (Mouse) 16590

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Function(s)

transmembrane receptor protein tyrosine kinase activity ATP binding cytokine binding metal ion binding nucleotide binding protease binding protein kinase activity protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity stem cell factor receptor activity protein binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein homodimerization activity transmembrane signal receptor

Process(es)

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CD117 (c-Kit) Monoclonal Antibody (2B8), PE-Cyanine5, eBioscience™

CD117 (c-Kit) Antibody (15-1171-82) in Flow

Product Details

15-1171-82

Flow Cytometry (Flow)

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